Abstract：
:Angiogenesis after intracerebral hemorrhage (ICH) injury can effectively alleviate brain damage and improve neurological function. Hypoxia-inducible factor 2α (HIF-2α) is an important angiogenic regulator and exhibits protective effects in several neurological diseases; however, its role in ICH has not yet been reported. Hence, in the present study, we explored whether HIF-2α reduces ICH injury by promoting angiogenesis. In addition, we explored the role of the vascular endothelial growth factor (VEGF)/Notch pathway in HIF-2α-mediated angiogenesis. We injected 50 μL of autologous blood taken from the femoral artery into the right striatum of healthy male adult Sprague-Dawley rats to create an autologous-blood-induced rat model of ICH. Lentiviral vectors were injected to both overexpress and knock down HIF-2α expression. VEGF receptor 2 (VEGFR2) and Notch-specific inhibitors were injected intraperitoneally to block VEGFR2- and Notch-mediated signaling after lentiviral injections. Our data showed that HIF-2α overexpression reduced neurological-damage scores and brain-water content, suggesting it had a protective effect on ICH injury. In addition, overexpression of HIF-2α promoted angiogenesis, increased focal cerebral blood flow (CBF), and reduced neuronal damage, whereas HIF-2α knockdown resulted in the opposite effects. Furthermore, we found that HIF-2α-mediated angiogenesis was blocked by a Notch-specific inhibitor. Likewise, the HIF-2α-mediated increase in phospho-VEGFR-2, cleaved-Notch1 and Notch1 expression was reversed via a VEGFR2-specific inhibitor. Taken together, our results indicate that HIF-2α promotes angiogenesis via the VEGF/Notch pathway to attenuate ICH injury. Moreover, our findings may contribute to the development of a novel strategy for alleviating ICH injury via HIF-2α-mediated upregulation of angiogenesis.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Chen H,Xiao H,Gan H,Zhang L,Wang L,Li S,Wang D,Li T,Zhai X,Zhao Jdoi
10.1016/j.neuroscience.2020.07.010subject
Has Abstractpub_date
2020-11-10 00:00:00pages
206-218eissn
0306-4522issn
1873-7544pii
S0306-4522(20)30444-9journal_volume
448pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:We have examined the relationship between brain-derived neurotrophic factor gene expression in the hippocampus and memory retention as well as long-term potentiation of rats. One-way inhibitory avoidance learning was adopted as the behavioural paradigm. Results revealed that brain-derived neurotrophic factor messenger...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00325-4
更新日期：1998-02-01 00:00:00
abstract：:We have observed that in rat caudate-putamen nuclei, neurons immunolabeled for choline acetyltransferase were sometimes in direct apposition to unlabeled perikarya and dendrites [Pickel V. M. and Chan J. (1990) J. Neurosci. Res. 25, 263-280]. Similar juxtapositions between plasmalemmas of nerve cells each receiving in...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90341-k
更新日期：1991-01-01 00:00:00
abstract：:The experiments described herein present a method for tracking diffusion of the glucocorticoid receptor agonist RU28362 in brain following i.c.v. drug administration. A useful property of glucocorticoid receptor is that it is primarily cytoplasmic when unbound and rapidly translocates to the nucleus when bound by liga...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.04.067
更新日期：2006-09-01 00:00:00
abstract：:Neurosteroids are synthesized de novo in the brain and the cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated that the rat Purkinje cell actively produces progesterone de novo from cholesterol only during neonatal life and progesterone promotes dendritic growth, spinogenesis and ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.04.003
更新日期：2004-01-01 00:00:00
abstract：:The distribution of wheatgerm agglutinin-horseradish peroxidase-labelled mossy fibre terminals of internal and external cuneate projections to the cerebellar anterior and posterior lobes were quantitatively analysed in adult rats. Computer-based image analysis mapped the spatial distribution of labelled cuneocerebella...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00081-x
更新日期：1997-09-01 00:00:00
abstract：:The aged dog brain accumulates beta-amyloid in the form of diffuse senile plaques, which provides a potentially useful in vivo model system for studying the events surrounding the deposition of beta-amyloid. We used postembedding immunocytochemistry at the electron microscopic level to determine the subcellular distri...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00568-0
更新日期：2000-01-01 00:00:00
abstract：:The present research explored the cortical correlates of emotional memories in response to words and pictures. Subjects' performance (Accuracy Index, AI; response times, RTs; RTs/AI) was considered when a repetitive Transcranial Magnetic Stimulation (rTMS) was applied on the left dorsolateral prefrontal cortex (LDLPFC...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2014.12.015
更新日期：2015-02-26 00:00:00
abstract：:The NMDA receptor (NMDAr) hypofunction theory of schizophrenia suggests that aberrant signaling through NMDAr underlies the pathophysiology of this disease. This is commonly modeled in rodents via treatment with NMDAr antagonists, which causes a range of behavioral effects that represent endophenotypes related to schi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.06.039
更新日期：2017-09-01 00:00:00
abstract：:Antisera raised against protein-glutaraldehyde-amino acid conjugates were used to study the light and electron microscopic distribution of GABA, glutamate, glutamine and taurine in organotypic slice cultures of rat hippocampi. In the stratum oriens and radiatum, glutamate-like immunoreactivity was particularly concent...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90187-7
更新日期：1992-01-01 00:00:00
abstract：:The posterior parahippocampal gyrus (PPHG) of the non-human primate brain has a distinct dual role in cortical neural systems. On the one hand, it is a critical link in providing the entorhinal cortex and hippocampal formation with cortical input, while on the other hand it receives output from these structures and pr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2008.03.077
更新日期：2008-06-23 00:00:00
abstract：:Production of tissue inhibitors of matrix metalloproteinases (TIMPs), a family of secreted proteins with inhibitory actions on matrix metalloproteinases (MMPs), is up-regulated following nerve injuries and is suggested to have protective effects against MMP-mediated tissue damages. To clarify the extracellular signals...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2007.04.047
更新日期：2007-07-13 00:00:00
abstract：:Peripheral neuropathy represents an important complication of diabetes involving a spectrum of structural, functional and biochemical alterations in peripheral nerves. Recent observations obtained in our laboratory have shown that the levels of neuroactive steroids present in the sciatic nerve of rat raised diabetic b...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.08.005
更新日期：2009-12-01 00:00:00
abstract：:The bladder and distal colon are innervated by lumbar splanchnic (LSN) and pelvic nerves (PN) whose axons arise from dorsal root ganglia (DRG) neurons at thoracolumbar (TL) and lumbosacral (LS) spinal levels, respectively. In an attempt to understand the molecular basis of differences between LSN and PN mechanosensiti...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.04.049
更新日期：2011-07-14 00:00:00
abstract：:The development of the catecholaminergic system in the hypothalamus and in the septal region was studied in rats from the 12th fetal day until the 9th postnatal day. Catecholaminergic structures were visualized with pre-embedding immunocytochemistry using antiserum to tyrosine hydroxylase. An intensification of diamin...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(89)90338-2
更新日期：1989-01-01 00:00:00
abstract：:Resident microglia are involved in immune responses of the central nervous system and may contribute to neuronal degeneration and death. Here, we tested in adult rats whether injection of bacterial lipopolysaccharide (which causes inflammation and microglial activation) just above the substantia nigra, results in the ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00033-6
更新日期：2000-01-01 00:00:00
abstract：:Neurones in the lumbosacral spinal cord are known to play a significant role in ejaculation. In these same areas neurones containing nitric oxide synthase (NOS) have been described. This raised the question as to whether there is a physiological role for nitrous oxide (NO) in the spinal cord in sexual behavior. We fir...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2007.09.010
更新日期：2007-12-05 00:00:00
abstract：:Interactions between neurotransmitters and the immune system represent new prospects for understanding neuroinflammation and associated neurological disease. GABA is the chief inhibitory neurotransmitter but its actions on immune pathways in the brain are unclear. In the present study, we investigated GABAergic transp...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2014.04.037
更新日期：2014-07-25 00:00:00
abstract：:During postnatal development, clusters of cells associated with the mononuclear phagocytic system appear within the white matter of rodents and cats. We studied the distribution and morphology of these cells in the hamster's brain during the first 2 weeks after birth. In animals of different ages, horseradish peroxida...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(85)90263-5
更新日期：1985-08-01 00:00:00
abstract：:Adolescent alcohol exposure (AAE) may exert long-term effects on the adult brain. Here, we tested the hypothesis that the brain regions affected include the rat hypothalamic-pituitary-adrenal (HPA) axis. Specifically, we examined the consequences of AAE [postnatal days (PND) 28-42] on the HPA axis-related brain circui...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.03.003
更新日期：2011-05-19 00:00:00
abstract：:The present study examined the antihyperalgesic effect of a specific inhibitor of Glycogen Synthase Kinase 3 (GSK3), AR-A014418, on the partial ligation of the sciatic nerve (PSNL), a neuropathic pain model in mice and investigated some mechanisms of action. AR-A014418 (0.01-1 mg/kg) administered by intraperitoneal ro...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.09.020
更新日期：2012-12-13 00:00:00
abstract：:The distribution and staining pattern of gamma-aminobutyric acid immunoreactivity have been examined by both light and electron microscopy in the dorsal part of the lateral geniculate nucleus of three reptilian species: the turtle Chinemys reevesi, the lizard Ophisaurus apodus and the snake Vipera aspis. After perfusi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90254-y
更新日期：1992-01-01 00:00:00
abstract：:Using arsenazo III as an intracellular indicator to monitor the calcium transients elicited by voltage-clamp depolarizing pulse, the effect of prolonged in vitro lithium treatment on excitation--contraction coupling in frog twitch muscle fibres was investigated. Incubation in 10 mM Li+ Ringer's solution for 2 days cau...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(90)90240-5
更新日期：1990-01-01 00:00:00
abstract：:Interleukin-1beta is a relatively small and abundant polypeptide that plays diverse roles in the central nervous system. In the present study, patterns of interleukin-1beta expression were observed in the olfactory bulbs of rats that had either undergone unilateral closure of the external naris or sham surgery on post...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00093-7
更新日期：1999-01-01 00:00:00
abstract：:Pituitary adenylate-cyclase activating polypeptide (PACAP) has been implicated in the (patho)physiology of stress-adaptation. PACAP deficient (PACAP(-/-)) mice show altered anxiety levels and depression-like behavior, but little is known about the underlying mechanisms in stress-related brain areas. Therefore, we aime...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.11.046
更新日期：2012-01-27 00:00:00
abstract：:The pedunculopontine nucleus (PPN) shows altered electrophysiological and anatomic characteristics in Parkinson's disease (PD), but little is known about the effect of 6-hydroxydopamine (6-OHDA) lesion and levodopa (L-DOPA) therapy on the relationship between spike and local field potential (LFP) activities in the PPN...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.01.044
更新日期：2019-04-15 00:00:00
abstract：:Using indirect immunofluorescence technique combined with retrograde tracing as well as surgical lesions, a system of spinothalamic neurons containing both galanin- and cholecystokinin-like immunoreactivity has been defined. The cell bodies are located in the lumbar segments L1-L5 with a preferential localization dors...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(87)90103-5
更新日期：1987-02-01 00:00:00
abstract：:Nitric oxide synthase-like immunoreactivity was found in a subpopulation of sympathetic postganglionic neurons in the cat stellate and lower lumbar ganglia. In the ganglia of other segments such cells were rare. Double staining for tyrosine hydroxylase-like immunoreactivity and nitric oxide synthase-like immunoreactiv...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00143-7
更新日期：1995-09-01 00:00:00
abstract：:Spreading depolarizations are accompanied by transient changes in cerebral blood flow (CBF). In a post hoc analysis of previously studied control rats we analyzed CBF time courses after middle cerebral artery occlusion in the rat in order to test whether intra-ischemic flow, reperfusion, and different parameters of pe...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.08.049
更新日期：2012-12-13 00:00:00
abstract：:Neural injury triggers changes in the expression of a large number of gene families. Particularly interesting are those encoding proteins involved in the generation, propagation or restoration of electric potentials. The expression of the Na+, K+-ATPase subunit isoforms (alpha, beta and gamma) was studied in dorsal ro...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.08.041
更新日期：2004-01-01 00:00:00
abstract：:The distribution of D1 dopamine receptors was studied autoradiographically in the basal ganglia of the cat, monkey and human. These receptor binding sites were labeled directly with the D1-selective antagonist [3H]SCH 23390, and ligand-binding assays were performed concurrently. Serial- or same-action analysis permitt...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(88)90130-3
更新日期：1988-07-01 00:00:00
