Starch granular protein of high-amylose wheat gives innate resistance to amylolysis.

Abstract:

:Granular protein is an important structural feature in determining starch digestibility. High-amylose wheat starch (HAWS) with >80% amylose content contains more granular protein than wild-type starch. As analyzed by mass spectrometry-based proteomics, granular-bound starch synthase (GBSS) is the major granular protein in isolated starch materials. GBSS content increases with amylose content (Spearman's correlation, p < 0.05), whereas the abundance relative to other proteins is similar among starches. Multiple amylase inhibitors were also identified. From Michaelis-Menten analysis, HAWS has a similar Km (Michaelis constant) as wild type, suggesting initial enzymatic binding is similar. After the pre-digestion of proteins, wild type had a greater change in starch digestibility than HAWS, probably due to the latter having 'thicker' granular-protein layers and higher enzymatic resistance of substrate per se. Overall, the study suggests that the greater granular protein content in HAWS is a factor that contributes to slower amylolysis compared to wild type.

journal_name

Food Chem

journal_title

Food chemistry

authors

Li HT,Sartika RS,Kerr ED,Schulz BL,Gidley MJ,Dhital S

doi

10.1016/j.foodchem.2020.127328

subject

Has Abstract

pub_date

2020-11-15 00:00:00

pages

127328

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(20)31190-0

journal_volume

330

pub_type

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