Abstract：
:In this study, the extraction, identification, and antialgal activity of toxic organic substances from decaying green alga Ulva prolifera, a disaster-causing alga on green tides in the Yellow Sea, were investigated. The toxic organic substances of U. prolifera were isolated by a sequence of procedures, namely, liquid-liquid extraction (LLE), silica gel chromatography column separation (SGCC), C18 solid phase extraction (C18 SPE), and acid-alkali separation (AAS) and their antialgal activities were tested against diatom Skeletonema costatum. The results showed that the 96h-EC50 value in each procedure was 57.00 mg•L-1, 12.14 mg•L-1, 11.70 mg•L-1, and 6.31 mg•L-1 in turn. Additionally, the initial (ethyl acetate extract in LLE) and final (Organic phase A in AAS) yield of toxic organics were 318 mg•kg-1 and 9 mg•kg-1 (the wet weight of U. prolifera). The final active fractions were preliminary identified as five 16- and 18-carbon fatty acids and four enols using GC-MS. The study may further evaluate the effect of bioactive substances on marine life after U. prolifera green tides bloom.
journal_name
Harmful Algaejournal_title
Harmful algaeauthors
Gao H,Su R,Zhou F,Zhang C,Shi Xdoi
10.1016/j.hal.2020.101786subject
Has Abstractpub_date
2020-03-01 00:00:00pages
101786eissn
1568-9883issn
1878-1470pii
S1568-9883(20)30066-4journal_volume
93pub_type
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