Interaction between cyanophage MaMV-DC and eight Microcystis strains, revealed by genetic defense systems.

Abstract:

:Cyanophage MaMV-DC is a member of Myoviridae that was reported to specifically infect and lyse Microcystis aeruginosa FACHB-524 among 21 selected cyanobacterial strains. We reidentified the infection specificity of MaMV-DC among seven other Microcystis strains of different species. In our experiments, MaMV-DC infected three Microcystis strains but did not form plaque in Microcystis lawns. This indicated that MaMV-DC is at least a genus- rather than strain-specific virus. Cyanophage MaMV-DC genes were transcribed in M. aeruginosa FACHB-524, M. flos-aquae TF09, M. aeruginosa TA09 and M. wesenbergii DW09, and the growth of these Microcystis strains was inhibited by the addition of MaMV-DC. The predicted defense of eight Microcystis strains by CRISPR-Cas systems has shown mixed consistency with the infection experiment results, suggesting other defense or anti-defense systems play roles during infection process. Restriction-modification (RM) system analysis revealed an abundance of four types of RM proteins that may play roles in defense against cyanophages.

journal_name

Harmful Algae

journal_title

Harmful algae

authors

Wang J,Bai P,Li Q,Lin Y,Huo D,Ke F,Zhang Q,Li T,Zhao J

doi

10.1016/j.hal.2019.101699

subject

Has Abstract

pub_date

2019-05-01 00:00:00

pages

101699

eissn

1568-9883

issn

1878-1470

pii

S1568-9883(19)30172-6

journal_volume

85

pub_type

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