Abstract：
:Long non-coding RNA MALAT1 was previously revealed to express abnormally in animal and cellular models of stroke, suggesting its indispensable role in stroke. The aims of the present study were to further investigate the functions of MALAT1 and to elucidate the underlying molecular mechanisms. Oxygen glucose deprivation/re-oxygenation (OGD/R) challenge was used in human brain microvascular endothelial cells (HBMECs) to mimic stroke injury in vitro. MALAT1 and miR-205-5p expression levels were evaluated by qRT-PCR. A tube formation assay was employed to verify the angiogenesis of HBMECs. Cell proliferation and apoptosis were evaluated using the ErdU assay and flow cytometry analysis, respectively. The interaction between miR-205-5p and MALAT1 was verified by dual-luciferase reporter assay. MALAT1 and miR-205-5p were both significantly upregulated in the serum of CIS patients and HBMECs under OGD/R, and the tube formation of HBMECs was damaged after OGD/R treatment. Silencing miR-205-5p remarkably promoted HBMEC proliferation and angiogenesis to resist OGD/R injury. Knockdown of MALAT1 markedly inhibited HBMEC proliferation and angiogenesis, and meanwhile promoted apoptosis induced by OGD/R treatment. Most importantly, MALAT1 acted as a competing endogenous RNA (ceRNA) of miR-205-5p via direct bonding with each other in HBMECs under OGD/R damage, indirectly upregulating the downstream targeted gene VEGFA. MALAT1 protected the angiogenesis function of HBMECs under OGD/R conditions by interacting with miR-205-5p/VEGFA pathway.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Gao C,Zhang CC,Yang HX,Hao YNdoi
10.1016/j.neuroscience.2020.03.027subject
Has Abstractpub_date
2020-05-21 00:00:00pages
135-145eissn
0306-4522issn
1873-7544pii
S0306-4522(20)30179-2journal_volume
435pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:We have developed a specific radioimmunoassay for "cerebellin", a 16-amino acid peptide recently isolated from rat cerebellum. In both rat and guinea-pig, cerebellin-like immunoreactivity was highest in the cerebellum but was also present in high concentrations elsewhere in the central nervous system, especially in th...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(88)90262-x
更新日期：1988-05-01 00:00:00
abstract：:Intrathecally-administered endomorphin-1 and endomorphin-2 produce antinociceptive effects which are different from each other. In order to elucidate a cellular basis for this result, we examined the effects of endomorphin-1 and endomorphin-2 on holding currents and spontaneous glutamatergic excitatory transmission in...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.01.010
更新日期：2006-01-01 00:00:00
abstract：:The nicotinic antagonists d-tubocurarine and trimethaphan camsylate competitively inhibit GABA-induced currents. Hexamethonium, mecamylamine and dihydro-beta-erythroidine, other nicotinic antagonists, do not affect GABA-elicited currents. The trimethaphan effect is completely reversed by a putative convulsant receptor...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00011-7
更新日期：1995-07-01 00:00:00
abstract：:Endomorphin 2 (EM2) plays essential roles in regulating nociceptive transmission within the spinal dorsal horn, where EM2-immunopositive (EM2-IP) fibers and terminals are densely encountered. However, the origins of these EM2-IP structures are still obscure. Unilateral primary sensory afferents disruption (lumbar 3-6 ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.05.006
更新日期：2010-08-11 00:00:00
abstract：:Latent inhibition, the process whereby pre-exposure to a conditioned stimulus without consequence impairs subsequent learning of an association between the conditioned stimulus and an unconditioned stimulus, is reportedly disrupted in both amphetamine-treated rats and in acute schizophrenics. This has led to the sugge...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00043-9
更新日期：2000-01-01 00:00:00
abstract：:Neurosteroids such as dehydroepiandrosterone (DHEA), pregnenolone and 17beta-estradiol are synthesized by cytochrome P450s from endogenous cholesterol. We previously reported a new cytochrome P450 enzyme, CYP7B, highly expressed in rat and mouse brain that metabolizes DHEA and related steroids by hydroxylation at the ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(03)00438-x
更新日期：2003-01-01 00:00:00
abstract：:In the latero-anterior hypothalamus (LAH) increased glutamate and vasopressin (AVP) activity facilitate anabolic androgenic steroid (AAS)-induced offensive aggression. In addition, adolescent AAS treatment increases the strength of glutamate-mediated connections between the LAH and the brain nucleus of stria terminali...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.03.056
更新日期：2011-06-30 00:00:00
abstract：:The hippocampus is a multifaceted, complex brain structure considered to be the learning center. The use of primary hippocampal cell cultures has uncovered important cellular mechanisms involved in overall physiological function. Yet, the use of primary culture is inherently difficult, and the lack of immortalized cel...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.06.076
更新日期：2010-09-29 00:00:00
abstract：:Midbrain dopamine neurons are critical in mediating the rewarding effects of opiates in dependent rats, as well as modulating some manifestations of opiate withdrawal. Morphine is known to excite dopamine neurons and thereby facilitate forebrain dopamine transmission through inhibition of GABA neurons. Cholinergic neu...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2005.08.035
更新日期：2005-01-01 00:00:00
abstract：:Receptor-associated protein appears to play an important role in low-density lipoprotein receptor-related protein trafficking. Since ligands for the low-density lipoprotein receptor-related protein have been implicated in Alzheimer's disease and normal functioning of this protein is indispensable for central nervous s...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00272-3
更新日期：1999-01-01 00:00:00
abstract：:The influence of intra-arterial injections of 5-hydroxytryptamine, histamine and prostaglandin E2 on afferent impulse activity of mesenteric nerves of small intestine was studied. In anaesthetized cats 5-hydroxytryptamine (10(-5)-10(-4) M) and histamine (10(-5)-10(-3) M) were shown to increase the impulse activity in ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00479-3
更新日期：1996-04-01 00:00:00
abstract：:Progestins mediate the onset and duration of lordosis, the mating posture of female rodents, through actions in the hypothalamus and ventral tegmental area. In the hypothalamus, progesterone has traditional, "genomic" actions via intracellular progestin receptors. In the ventral tegmental area, 3alpha-hydroxy-5alpha-p...
journal_title：Neuroscience
pub_type： 杂志文章,评审
doi：10.1016/j.neuroscience.2005.06.015
更新日期：2006-01-01 00:00:00
abstract：:The presence of subgroups in vomeronasal sensory neurons has been known in various animals. To elucidate possible functional subdivisions in the guinea-pig accessory olfactory bulb, the combined studies with GTP-binding protein immunohistochemistry, electrophysiological and optical recordings were carried out. Gi2 alp...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00690-2
更新日期：1997-08-01 00:00:00
abstract：:The posterior parahippocampal gyrus (PPHG) of the non-human primate brain has a distinct dual role in cortical neural systems. On the one hand, it is a critical link in providing the entorhinal cortex and hippocampal formation with cortical input, while on the other hand it receives output from these structures and pr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2008.03.077
更新日期：2008-06-23 00:00:00
abstract：:Electric current has been used for epilepsy treatment by targeting specific neural circuitries. Despite its success, direct contact between the electrode and tissue could cause side effects including pain, inflammation, and adverse biological reactions. Magnetic stimulation overcomes these limitations by offering adva...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.02.018
更新日期：2020-04-15 00:00:00
abstract：:We have previously reported that repeated central administration of sub-anxiogenic doses of the corticotropin releasing factor 1 (CRF(1)) agonist Cortagine, termed "priming," elicits a phenotype of increased anxiety-like behaviors in the elevated plus maze (EPM) and open-field test, and enhanced retention of contextua...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.04.012
更新日期：2009-08-04 00:00:00
abstract：:Purinergic signaling through activation of P2X and P2Y receptors is critically important in the chemical senses. In the mouse main olfactory epithelium (MOE), adenosine 5'-triphosphate (ATP) elicits an increase in intracellular calcium ([Ca(2+)](I)) and reduces the responsiveness of olfactory sensory neurons to odoran...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.06.004
更新日期：2012-09-18 00:00:00
abstract：:The goal of the present experiments was to examine the relationships of the zona incerta with two structures associated with visuomotor behavior, the superior colliculus and pretectum. The experiments were carried out in the cat, a species commonly used in studies of visuomotor integration, and utilized wheat germ agg...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00535-0
更新日期：1997-04-01 00:00:00
abstract：:Recent evidence suggests that ischemia/reperfusion (I/R) in an organ may have distance effect on the brain. In this study, the effects of renal I/R, limb I/R or both together on the structural and function of hippocampus were evaluated and compared. Hence, rats were subjected to 2-h bilateral lower limb ischemia, 45-m...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.06.058
更新日期：2017-09-01 00:00:00
abstract：:Noradrenergic neurons from the locus coeruleus innervate several brain regions, such as hippocampus and cortex. The hippocampus exhibits the highest concentration of the neurotrophins nerve growth factor, brain-derived neurotrophic factor and neurotrophin-3 in the brain. To study the role of the noradrenergic system i...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00346-k
更新日期：1996-01-01 00:00:00
abstract：:The changes of electrical polarization of primary afferent terminals in the lumbosacral spinal cord have been investigated on immobilized, decorticated and spinal cats during fictive locomotion. Fictive locomotion was spontaneous or provoked by stimulation of either dorsal root or dorsal funiculi in the lumbar segment...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(82)90253-6
更新日期：1982-06-01 00:00:00
abstract：:Several experimental models of peripheral neuropathy show that a significant upregulation of spinal dynorphin A and its precursor peptide, prodynorphin, is a common consequence of nerve injury. A genetically modified mouse strain lacking prodynorphin does not exhibit sustained neuropathic pain after nerve injury, supp...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2003.08.021
更新日期：2004-01-01 00:00:00
abstract：:The adverse consequences of early-life sleep deprivation on mental health are well recognized, yet many aspects remain unknown, therefore, animal studies can offer useful insights. Male Sprague-Dawley rats at postnatal day (PND) 19 were subjected to sleep deprivation (SD) for 14 days (6-8 hours/day). Control (CON) rat...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.02.021
更新日期：2019-05-15 00:00:00
abstract：:Anatomical and electrophysiological evidence has contributed to the hypothesis that microzones in the mammalian flocculus are organized to reflect control of eye movements in the planes of semicircular canals. Adult male Long-Evans rats received iontophoretic injections of FluoroGold and/or tetramethylrhodamine dextra...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00232-3
更新日期：2000-01-01 00:00:00
abstract：:Hypocretin/orexin (Hcrt/Orx) and melanin concentrating hormone (MCH) are peptides contained in overlapping cell groups of the lateral hypothalamus and commonly involved in regulating sleep-wake states and energy balance, though likely in different ways. To see if these neurons are similarly or differentially modulated...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.10.032
更新日期：2005-01-01 00:00:00
abstract：:Previous investigations of cortical organization in the brain of the turtle have revealed many features typical of mammalian neocortex. Recent evidence suggests that many neocortical neurons contain neuroactive peptides. The possibility that one such peptide, somatostatin, is found in the turtle brain was tested using...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(83)90295-6
更新日期：1983-06-01 00:00:00
abstract：:Extracellular nucleotides exert their actions via two subfamilies of purinoceptors: P2X and P2Y. Eight mammalian P2Y receptor subtypes (P2Y(1,2,4,6,11,12,13,14)) have been identified. In this work, the localization of P2Y(6) was studied in rat retina using double immunofluorescence labeling and confocal scanning micro...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2012.06.032
更新日期：2012-09-18 00:00:00
abstract：:Nitric oxide has been proposed to act as an intercellular messenger in central brainstem circuits controlling gastrointestinal motility. In particular, a subpopulation of preganglionic vagal neurons of the dorsal motor nucleus of the vagus have been shown to be reduced nicotinamide adenine dinucleotide phosphate(NADPH...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00586-7
更新日期：1999-05-01 00:00:00
abstract：:Pain is processed in a large neural network that partially overlaps structures involved in emotion processing. Despite the fact that pain and emotion are known to share neural regions and interact in numerous clinical conditions, relatively little is known about the interaction of pain and emotion at the neural level....
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.02.047
更新日期：2017-05-04 00:00:00
abstract：:In rodents, chronic intermittent ethanol vapor exposure (CIE) produces alcohol dependence, alters the structure and activity of pyramidal neurons and decreases the number of oligodendroglial progenitors in the medial prefrontal cortex (mPFC). In this study, adult Wistar rats were exposed to seven weeks of CIE and were...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.02.043
更新日期：2015-05-07 00:00:00
