Reconstructing the Phylogeny of Corynebacteriales while Accounting for Horizontal Gene Transfer.

Abstract:

:Horizontal gene transfer is a common mechanism in Bacteria that has contributed to the genomic content of existing organisms. Traditional methods for estimating bacterial phylogeny, however, assume only vertical inheritance in the evolution of homologous genes, which may result in errors in the estimated phylogenies. We present a new method for estimating bacterial phylogeny that accounts for the presence of genes acquired by horizontal gene transfer between genomes. The method identifies and corrects putative transferred genes in gene families, before applying a gene tree-based summary method to estimate bacterial species trees. The method was applied to estimate the phylogeny of the order Corynebacteriales, which is the largest clade in the phylum Actinobacteria. We report a collection of 14 phylogenetic trees on 360 Corynebacteriales genomes. All estimated trees display each genus as a monophyletic clade. The trees also display several relationships proposed by past studies, as well as new relevant relationships between and within the main genera of Corynebacteriales: Corynebacterium, Mycobacterium, Nocardia, Rhodococcus, and Gordonia. An implementation of the method in Python is available on GitHub at https://github.com/UdeS-CoBIUS/EXECT (last accessed April 2, 2020).

journal_name

Genome Biol Evol

authors

Coimbra NDR,Goes-Neto A,Azevedo V,Ouangraoua A

doi

10.1093/gbe/evaa058

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

381-395

issue

4

issn

1759-6653

pii

5809532

journal_volume

12

pub_type

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