Abstract:
:Osteosarcoma, one of the most common malignant bone tumors, is characterized by a high rate of metastasis, and the survival rate of patients with metastatic osteosarcoma is poor. Previous studies have reported that miRNAs often regulate the occurrence and development of various tumors. In this work, we identified miRNA-542-5p as a critical miRNA in osteosarcoma by overlapping three Gene Expression Omnibus datasets, and then evaluated miRNA-542-5p expression profiles using Gene Expression Omnibus and Sarcoma-microRNA Expression Database. We used MISIM to investigate miRNAs correlated with miR-542 and identified potential target genes of miRNA-542-5p using miRWalk. Functional and pathway enrichment analyses were performed using The Database for Annotation, Visualization and Integrated Discovery. Protein-protein interaction was performed using Search Tool for the Retrieval of Interacting Genes and Cytoscape. We report that the relative level of miRNA-542-5p was significantly higher in osteosarcoma than in healthy bone. Expressions of hsa-miR-330 and hsa-miR-1202 were found to be strongly correlated with that of miR-542-5p. Furthermore, we identified a total of 514 down-regulated genes as possible targets of miR-542-5p. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis demonstrated that the putative target genes of miR-542-5p were most enriched in the cell-cycle process. The differentially expressed genes CDCA5, PARP12 and HSPD1 were found to be hub genes in protein-protein interaction networks. Finally, transfection of the osteosarcoma cell line U2OS with miR-542-5p mimics or inhibitor revealed that miR-542-5p can promote cell proliferation. In conclusion, our results suggest that miR-542-5p may promote osteosarcoma proliferation; thus, this miRNA may have potential as a biomarker for diagnosis and prognosis.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Zhu T,Fan D,Ye K,Liu B,Cui Z,Liu Z,Tian Ydoi
10.1002/2211-5463.12824subject
Has Abstractpub_date
2020-04-01 00:00:00pages
627-636issue
4issn
2211-5463journal_volume
10pub_type
杂志文章相关文献
FEBS Open Bio文献大全abstract::The tumour microenvironment comprises a diverse range of cells, including fibroblasts, immune cells and endothelial cells, along with extracellular matrix. In particular, fibroblasts are of significant interest as these cells are reprogrammed during tumorigenesis to become cancer-associated fibroblasts (CAFs), which i...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12831
更新日期:2020-05-01 00:00:00
abstract::Neural stem cells (NSC) from the adult hippocampus easily lose their activity in vitro. Efficient in vitro expansion of adult hippocampus-derived NSC is important for generation of tools for research and cell therapy. Here, we show that a single copy disruption or pharmacological inhibition of p38α enables successful ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.05.001
更新日期:2015-05-08 00:00:00
abstract::Synthetic biology circuits are often constructed with multiple gene expression units assembled in close proximity, and they can be used to perform complex functions in embryonic stem cells (ESCs). However, mutual interference between transcriptional units has not been well studied in mouse ESCs. To assess the efficien...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12818
更新日期:2020-04-01 00:00:00
abstract::Mutations in the eye lens gap junction protein connexin 50 cause cataract. Earlier we identified a frameshift mutant of connexin 50 (c.670insA; p.Thr203AsnfsX47) in a family with autosomal recessive cataract. The mutant protein is smaller and contains 46 aberrant amino acids at the C-terminus after amino acid 202. Her...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2012.11.005
更新日期:2012-11-27 00:00:00
abstract::Acute lung injury (ALI) is accompanied by overactivation of multiple pro-inflammatory factors. Cytochrome P450 1A1 (CYP1A1) has been shown to aggravate lung injury in response to hyperoxia. However, the relationship between CYP1A1 and lipopolysaccharide (LPS)-induced ALI is unknown. In this study, CYP1A1 was shown to ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12977
更新日期:2020-09-15 00:00:00
abstract::Megakaryoblastic leukemia 1 (MKL1) is a member of the MKL family of serum response factor (SRF) coactivators. Here we have identified three rat MKL1 transcripts: two are homologues of mouse MKL1 transcripts, full-length MKL1 (FLMKL1) and basic, SAP, and coiled-coil domains (BSAC), the third is a novel transcript, MKL1...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.09.001
更新日期:2013-09-07 00:00:00
abstract::Glioma is one of the most common types of malignant primary central nervous system tumor, and prognosis for this disease is poor. As autophagic drugs have been reported to induce glioma cell death, we investigated the potential prognostic role of autophagy-associated long non-coding RNA (lncRNA) in glioma patients. In...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12601
更新日期:2019-03-05 00:00:00
abstract::Dedifferentiation of adipocytes contributes to the generation of a proliferative cell population that could be useful in cellular therapy or tissue engineering. Adipocytes can dedifferentiate into precursor cells to acquire a fibroblast-like phenotype using ceiling culture, in which the buoyancy of fat cells is exploi...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12250
更新日期:2017-07-10 00:00:00
abstract::Identifying conserved binding motifs is an efficient way to study protein-ligand recognition. Most 3D binding motifs only contain information from the protein side, and so motifs that combine information from both protein and ligand sides are desired. Here, we propose an algorithm called LibME (Ligand-binding Motif Ex...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12150
更新日期:2016-11-30 00:00:00
abstract::The extreme halophile Halococcus morrhuae (ATCC® 17082) contains a 108-nucleotide insertion in its 5S rRNA. Large rRNA expansions in Archaea are rare. This one almost doubles the length of the 5S rRNA. In order to understand how such an insertion is accommodated in the ribosome, we obtained a cryo-electron microscopy ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12962
更新日期:2020-10-01 00:00:00
abstract::Shiga toxin (Stx) causes fatal systemic complications. Stx induces apoptosis, but the mechanism of which is unclear. We report that Stx induced rapid reduction of short-lived anti-apoptotic proteins followed by activation of caspase 9 and the progression of apoptosis. Proteasome inhibitors prevented the reduction of a...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.06.005
更新日期:2015-06-12 00:00:00
abstract::Epigallocatechin gallate (EGCG), a major polyphenol in green tea, possesses antioxidant properties and regulates various cell functions. Here, we examined the function of EGCG in inflammatory bone resorption. In calvarial organ cultures, lipopolysaccharide (LPS)-induced bone resorption was clearly suppressed by EGCG. ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.06.003
更新日期:2015-06-12 00:00:00
abstract::Obesity during pregnancy has been shown to increase the risk of metabolic diseases in the offspring. However, the factors within the maternal milieu which affect offspring phenotypes and the underlying mechanisms remain unknown. The adipocyte hormone leptin plays a key role in regulating energy homeostasis and is know...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12757
更新日期:2020-01-01 00:00:00
abstract::The A/B domains of nuclear receptors such as thyroid receptor α (TRα) are considered to be conformationally flexible and can potentially adopt multiple structural conformations. We used intrinsic tryptophan fluorescence quenching and circular dichroism spectroscopy to characterize the unfolding of this A/B domain upon...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12229
更新日期:2017-05-15 00:00:00
abstract::As a small fruit rich in anthocyanins, blueberry (Vaccinium corymbosum × angustifolium) has become a focus of research in recent years for identifying genes related to anthocyanin transport and stress resistance mechanisms based on transcriptome sequencing. However, the lack of validated, stably expressed reference ge...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12903
更新日期:2020-08-01 00:00:00
abstract::Decorin (DCN) is a major member of the small leucine-rich proteoglycan (SLRP) family that is critically involved in tumorigenesis and the development of metastasis of cancers, including glioma. Overexpression of DCN was indicated to suppress glioma cell growth. However, the role of DCN in the migration of glioma cells...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12076
更新日期:2016-05-31 00:00:00
abstract::The proton-coupled folate transporter (PCFT, SLC46A1) transports folic acid across the plasma membrane, together with an excess of protons such that the net charge translocation is positive. We developed 3D structural models of PCFT threaded onto the X-ray structures of major facilitator superfamily (MFS) members that...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12041
更新日期:2016-02-22 00:00:00
abstract::Flavin adenine dinucleotide (FAD), synthesized from riboflavin, is redox cofactor in energy production and plays an important role in cell survival. More recently, riboflavin deficiency has been linked to developmental disorders, but its role in stem cell differentiation remains unclear. Here, we show that FAD treatme...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12331
更新日期:2017-10-17 00:00:00
abstract::Hirschmanniella mucronata is a plant-parasitic nematode that is widespread in rice production areas and causes 10-25% yield losses a year on average. Here, we investigated the mechanism of resistance to this nematode by comparing the transcriptomes of roots from resistant (Jiabali) and susceptible (Bawangbian) varieti...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12737
更新日期:2019-11-01 00:00:00
abstract::Insulin signaling plays a central role in the regulation of facilitative glucose transporters (GLUTs) in humans. To establish Caenorhabditis elegans (C. elegans) as a model to study the mechanism underlying insulin regulation of GLUT, we identified that FGT-1 is most likely the only functional GLUT homolog in C. elega...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12068
更新日期:2016-04-21 00:00:00
abstract::Redox-coupled folding pathways of bovine pancreatic ribonuclease A (RNase A) with four intramolecular disulfide (SS) bonds comprise three phases: (I) SS formation to generate partially oxidized intermediate ensembles with no rigid folded structure; (II) SS rearrangement from the three SS intermediate ensemble (3S) to ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2012.04.001
更新日期:2012-04-16 00:00:00
abstract::Angiotensin converting enzyme (ACE) is a zinc-dependent dipeptidyl carboxypeptidase with an essential role in blood pressure homeostasis in mammals. ACE has long been targeted in the treatment of hypertension through ACE inhibitors, however current inhibitors are known to cause severe side effects. Therefore, there is...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.08.004
更新日期:2015-08-11 00:00:00
abstract::The human MET receptor tyrosine kinase contributes to vertebrate development and cell proliferation. As a proto-oncogene, it is a target in cancer therapies. MET is also relevant for bacterial infection by Listeria monocytogenes and is activated by the bacterial protein internalin B. The processes of ligand binding, r...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12285
更新日期:2017-08-29 00:00:00
abstract::Eukaryotic protein O-mannosyltransferases (Pmts) are divided into three subfamilies (Pmt1, Pmt2, and Pmt4) and activity of Pmts in yeasts and animals requires assembly into complexes. In Saccharomyces cerevisiae, Pmt1 and Pmt2 form a heteromeric complex and Pmt 4 forms a homomeric complex. The filamentous fungus Asper...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.03.006
更新日期:2014-03-19 00:00:00
abstract::Peroxisome proliferator-activated receptor-gamma coactivator-1β (PGC-1β) is a transcriptional regulator whose increased expression activates energy expenditure-related genes in skeletal muscles. However, how PGC-1β is regulated remains largely unclear. Here, we show that PGC-1β gene expression is negatively correlated...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12898
更新日期:2020-07-01 00:00:00
abstract::Tropomyosins, a family of actin-associated proteins, bestow actin filaments with distinct biochemical and physical properties which are important for determining cell shape and regulating many cellular processes in eukaryotic cells. Here, we used RNA-seq to investigate the effect of four tropomyosin isoforms on gene e...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12386
更新日期:2018-02-19 00:00:00
abstract::Oxidized cholesterol (oxysterols) plays an important and multifaceted role in lipid metabolism. Here we examined whether dietary oxysterols accelerate hepatic lipid accumulation and inflammation in nonhuman primates. We also examined the effect of the Niemann-Pick C1-like1 inhibitor, ezetimibe (Ez). Macaca fasciculari...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12107
更新日期:2016-09-20 00:00:00
abstract::The expression of death receptor 6 (DR6) is abnormal in some cancer types, but the function and underlying molecular mechanisms of DR6 in tumor progression are not yet clear. In the present study, our analysis of ovarian cancer RNA sequencing data from The Cancer Genome Atlas revealed that DR6 is upregulated in human ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12492
更新日期:2018-08-07 00:00:00
abstract::Ki-67 (MKI67) is a marker of cellular proliferation of cancer. Here, we show that Ki-67 is post-transcriptionally regulated through alternative polyadenylation (APA) and microRNAs in breast cancer. We show that shortening of the Ki-67 3'UTR results in the loss of the binding sites for the suppressive miRNAs and thus r...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12364
更新日期:2018-01-26 00:00:00
abstract::Soft cheese with white rind lacks essential fatty acids (EFAs), and as a result its long-term consumption may lead to various kinds of cardiovascular and cerebrovascular diseases, such as hyperlipidemia, hypertension, and atherosclerosis. Geotrichum candidum is a dimorphic yeast that plays an important role in the rip...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12553
更新日期:2018-12-06 00:00:00