Abstract:
PURPOSE:To examine the intra- and inter-individual variability in fatty acid composition of follicular fluid (FF) of 23 patients undergoing assisted reproductive treatment. METHODS:The average coefficient of variation within each patient (CVw) and intra-class correlation coefficient (ICC) values of FF fatty acid composition as well as correlation between the fatty acid composition of individual, pooled or first-punctured follicles, were assessed. RESULTS:The proportions of 16:0, 18:0, cis-9 18:1, 18:2n-6, 20:5n-3, total MUFA and n-3 PUFA showed good reproducibility (CVw < 10%). Although CVw values of 18:3n-3 and 20:3n-6 exceeded 10%, variation between patients exceeded intra-individual variation as indicated by elevated ICC values (0.61 and 0.66, respectively). Nevertheless, 20:4n-6 and 22:6n-3 showed non-negligible intra-patient variation. With the exception of some minor fatty acids (< 0.30 g/100 g), strong relationships were demonstrated between the average proportion in individually analysed follicles and the proportion determined in pooled samples and in the first, largest follicle. CONCLUSION:The CVw and ICC values of proportions of 16:0, 18:0, cis-9 18:1, 18:2n-6, 18:3n-3, 20:5n-3, total MUFA and n-3 PUFA showed limited intra-individual variation and moderate to good reliability. However, this is not the case for some other PUFA, such as 20:4n-6 and 22:6n-3. Nevertheless, for all of these fatty acid(s) (groups), calculated average fatty acid proportions were highly correlated with proportions determined in pooled samples and in the first, largest follicle. This implies that single or pooled follicle aspiration suffices to assess intra-individual variation in the FF of these fatty acids.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Liu YJ,Vlaeminck B,Tilleman K,Chouinard PY,Gervais R,De Sutter P,Fievez Vdoi
10.1007/s10815-019-01669-6subject
Has Abstractpub_date
2020-03-01 00:00:00pages
649-655issue
3eissn
1058-0468issn
1573-7330pii
10.1007/s10815-019-01669-6journal_volume
37pub_type
杂志文章abstract:PURPOSE:To investigate the effect of the cumulative exposure to estradiol (E(2)) during the follicular phase on IVF outcome. METHODS:Patients were stimulated with recombinant FSH after GnRH agonist suppression and had a day 3-embryo transfer. Estrogen exposure was determined as the area under the curve (AUC) for serum...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-006-9101-x
更新日期:2007-04-01 00:00:00
abstract:PURPOSE:To elucidate the appropriateness of current indications for assisted hatching (AH) in cleavage stage human embryos and to confirm our preliminary findings that only young patients (about 67%) benefit from AH. METHODS:Prior to transfer, 2 of 3 embryos selected for ET were subjected to laser assisted hatching (L...
journal_title:Journal of assisted reproduction and genetics
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更新日期:2003-05-01 00:00:00
abstract::The rapid development of gene-editing technologies has led to an exponential rise in both basic and translational research initiatives studying molecular processes and investigating possible clinical applications. Early experiments using genome editing to study human embryo development have contradicted findings in st...
journal_title:Journal of assisted reproduction and genetics
pub_type: 社论
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更新日期:2018-08-01 00:00:00
abstract:PURPOSE:The aim of the present study was to determine whether supplementation of resveratrol, a stilbenoid antioxidant with therapeutic significance, influences goat (Capra hircus) oocyte maturation and subsequent embryonic development and expression of apoptosis and early embryonic development-related genes. METHODS:...
journal_title:Journal of assisted reproduction and genetics
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更新日期:2014-02-01 00:00:00
abstract::Infertility is a major health problem across the world. One of the main reasons for male infertility are defects in sperm. Semen analysis is the most common test utilized to evaluate male fertility and since it suffers from multiple drawbacks, reproduction scientists have tried to find new molecular markers for detect...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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abstract:PURPOSE:To correlate levels of cholesterol (CH), calcium (Ca2+), and mitochondrial activity (MA) with the standard semen parameters and to compare them between fertile and infertile men. METHODS:We studied 151 semen samples from infertile (n = 60) or fertile (n = 91) males. Basic sperm parameters were analyzed. Ca2+ a...
journal_title:Journal of assisted reproduction and genetics
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doi:10.1007/s10815-004-8761-7
更新日期:2004-12-01 00:00:00
abstract:PURPOSE:To study the presence and distribution of genes encoding free radical scavengers in human granulosa cells from primordial and primary ovarian follicles. METHODS:A class comparison study on existing granulosa cell transcriptome from primordial (n = 539 follicles) and primary (n = 261) follicles donated by three...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-018-1240-3
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:The aim was to evaluate the effect of aspirin on pregnancy and implantation rates in an unselected group of patients undergoing intracytoplasmic sperm injection (ICSI). METHODS:Two hundred and seventy-nine patients were randomized to receive 80 mg of aspirin (n = 139) or no treatment (r = 136) starting from th...
journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1023/a:1026491426423
更新日期:2000-11-01 00:00:00
abstract::The understanding of the embryo's nutrient requirements and physiology has led to the development of more physiological culture media, capable of supporting acceptable levels of human blastocyst development in vitro. The success of such media can be attributed to catering to the embryo's changing nutrient requirements...
journal_title:Journal of assisted reproduction and genetics
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abstract::Gender selection by PGD is an appropriate use of medical resources. Children borne through PGD for gender determination would be welcome and would come into a couple's life at a planned, opportune time. If the practice were made more available through insurance coverage, the size and makeup of families could become a ...
journal_title:Journal of assisted reproduction and genetics
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更新日期:2002-09-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1023/a:1021127410300
更新日期:1997-10-01 00:00:00
abstract:PURPOSE:The purpose of this study was to investigate the effect of male and female serum supplementation on the in vitro development of mouse embryos beyond the blastocyst stage until the outgrowth stage since the latter may be related to the nidation of the embryo. We also studied the effect of EGF addition on embryo ...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/BF02066499
更新日期:1996-11-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-010-9504-6
更新日期:2011-03-01 00:00:00
abstract:PURPOSE:To evaluate the association between serum progesterone (P) levels on the day of embryo transfer (ET) and pregnancy rates in fresh donor IVF/ICSI cycles. METHODS:Fresh donor cycles with day 3 ET from 10/2007 to 8/2012 were included (n = 229). Most cycles (93 %) were programmed with a gonadotropin releasing horm...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-014-0199-y
更新日期:2014-05-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-017-0965-8
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journal_title:Journal of assisted reproduction and genetics
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journal_title:Journal of assisted reproduction and genetics
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更新日期:2015-11-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2019-06-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1023/a:1014740906035
更新日期:2002-03-01 00:00:00
abstract:PURPOSE:The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue. METHODS:Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-013-0105-z
更新日期:2013-11-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-012-9868-x
更新日期:2012-11-01 00:00:00
abstract:PURPOSE:This study aimed to induce follicular wave emergence (FWE) using pharmacological (recombinant hCG administration) or mechanical (aspiration of dominant follicle) interventions in infertile women. METHODS:Sixteen infertile women (≤35 years) with indications for in vitro fertilization due to tubal and/or male fa...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,随机对照试验
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更新日期:2015-04-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2017-09-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1023/b:jarg.0000042008.83699.cc
更新日期:2004-07-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00