Abstract:
PURPOSE:To investigate the difference of in vitro and in vivo grown oocytes, we compared maturation, fertilization, development, and maternal gene expression from both in vitro and in vivo grown mouse oocytes. METHODS:The preantral follicles isolated from 12-day-old mice were cultured on Transwell-COL membrane inserts. After culture, maturation, fertilization, and developmental rates were assessed. RT-PCR (reverse transcription-polymerase chain reaction) was performed to examine the expression of beta-actin, GDF-9, and IGF-II in matured oocytes. RESULTS:No difference in the nuclear maturation was detected between in vitro and in vivo grown oocytes, but the mean oocyte diameter of the in vitro group was smaller than that of the in vivo group. The fertilization rate was significantly lower in the in vitro group than in the in vivo group (p < 0.05). The capacities of in vitro grown oocyte to cleave and develop to blastocysts were significantly lower than those of the in vivo grown oocytes (p < 0.001). Moreover, blastocyst of in vitro group had fewer total cells than those of in vivo group (p < 0.05). In regards to the expression of genes in mature oocytes, growth differentiation factor-9 (GDF-9) expression was similar between the two groups, but beta-actin was significantly reduced in the in vitro group compared to the in vivo group. Particularly, the expression of insulin-like growth factor II (IGF-II) was not found in the in vitro grown oocytes. CONCLUSIONS:These results showed that in vitro grown oocytes did not have the same developmental capacity as in vivo grown oocytes. We assume that the aberrant expression of maternal-derived genes in the in vitro grown oocytes may cause the poor embryo viability.
journal_name
J Assist Reprod Genetjournal_title
Journal of assisted reproduction and geneticsauthors
Kim DH,Ko DS,Lee HC,Lee HJ,Park WI,Kim SS,Park JK,Yang BC,Park SB,Chang WK,Lee HTdoi
10.1023/b:jarg.0000042008.83699.cckeywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
233-40issue
7eissn
1058-0468issn
1573-7330journal_volume
21pub_type
杂志文章abstract:PURPOSE:To evaluate if several genetic loci that are associated with variation in normal menopause age and early menopause can account for a poor response to controlled ovarian stimulation. METHODS:A total of 71 patients age ≤35 years old who were undergoing intracytoplasmic sperm injection were genotyped for four gen...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-012-9735-9
更新日期:2012-05-01 00:00:00
abstract:PURPOSE:This study sought to identify a disease-related gene in a consanguineous Chinese family in which there were two premature ovarian insufficiency (POI) sisters. METHOD:We used whole-exome sequencing and Sanger sequencing to identify the disease-causing gene. Results were verified using an assay of mutant protein...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-019-01675-8
更新日期:2020-02-01 00:00:00
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journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,评审
doi:10.1007/s10815-016-0663-y
更新日期:2016-04-01 00:00:00
abstract:PURPOSE:To elicit patient preferences for social media utilization and content in the infertility clinic. METHODS:This was a cross-sectional survey study conducted in three US fertility practices. Women presenting to the infertility clinic for an initial or return visit were offered an anonymous voluntary social media...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-018-1189-2
更新日期:2018-07-01 00:00:00
abstract:PURPOSE:In a preimplantation genetic diagnosis for aneuploidy (PGD-A) program, the more embryos available for biopsy, consequently increases the chances of obtaining euploid embryos to transfer. The aim was to increase the number of viable euploid blastocysts in patients undergoing PGD-A using fresh oocytes together wi...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-016-0868-0
更新日期:2017-04-01 00:00:00
abstract:PURPOSE:To examine the efficacy of low-dose hCG using a GnRH antagonist protocol. METHODS:Prospective randomized study was performed at the Kyono Ladies Clinic. One hundred ninety-two women (<40 -years old, <3 previous cycles) were randomly assigned to GnRH agonist (buserelin) long protocol (LP, n = 66), GnRH antagoni...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10815-006-9036-2
更新日期:2006-05-01 00:00:00
abstract:PURPOSE:The goals of this study are to analyze the clinical pregnancy rate as a function of the pre-intracytoplasmic sperm injection (ICSI) oocyte spindle angle and determine factors which can be associated with different spindle angles, if clinically relevant. METHODS:Fifty-eight patients, who underwent their first I...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-017-0890-x
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:The aim of this study was to investigate the association between two common single nucleotide polymorphisms (SNPs) in the vascular endothelial growth factor (VEGF) gene (-460C/T and +405G/C) and polycystic ovary syndrome (PCOS) risk in south Indian women. METHODS:This study involves clinically confirmed PCOS p...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-014-0310-4
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. METHODS:We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured ...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-016-0648-x
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:To review the history of experimental embryo culture and how culture media that permitted complete preimplantation development in vitro were first discovered, and the physiological insights gained. METHODS:This article reviews the history of in vitro mammalian embryo culture, in particular the efforts that led...
journal_title:Journal of assisted reproduction and genetics
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s10815-013-0095-x
更新日期:2013-08-01 00:00:00
abstract:PURPOSE:To review the current literature regarding the role of vitamin D status in pregnancy outcomes in women undergoing assisted reproductive technology (ART) and to assess cost-effectiveness of routine vitamin D deficiency screening and repletion prior to initiation of ART. METHODS:A systematic literature review wa...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章,评审
doi:10.1007/s10815-014-0407-9
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:The aim of the study was to assess cytogenetic and embryoscopic characteristics in subsequent miscarriages of spontaneous pregnancy losses (SPL) and recurrent pregnancy losses (RPL). METHODS:A retrospective cohort of 75 women was affected by repeated pregnancy loss. Of those, 34 had SPL, 24 primary RPL, and 17...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-018-1226-1
更新日期:2018-08-01 00:00:00
abstract:PURPOSE:The IGF signaling cascade exerts important regulatory functions in human ovarian folliculogenesis. The scope of this study was to evaluate the transcription profile of insulin-like growth factor (IGF) genes during human ovarian follicle development and to analyze follicle fluid levels of key IGF proteins. METH...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-019-01432-x
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:To assess the efficacy of a protocol involving the discontinuation of the GnRH analogue at the mid-phase of ovarian stimulation for IVF in patients with a previous poor response. METHODS:Prospective case-control evaluation compared with same patient's previous performance. Thirty-six patients enrolled in an IV...
journal_title:Journal of assisted reproduction and genetics
pub_type: 临床试验,杂志文章
doi:10.1023/a:1009476411762
更新日期:2001-04-01 00:00:00
abstract:PURPOSE:To evaluate clinical outcomes of frozen-thawed embryo transfer cycles when one or two blastocysts are transferred. METHODS:Retrospective chart review RESULTS:Two hundred forty-three frozen blastocyst transfer (FBT) cycles were analyzed. Clinical pregnancy rate (50.4% vs. 34.7%), live birth rate (45.8% vs. 30....
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-011-9551-7
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:The basic semen parameters seem to have a limited predictive value in male fertility. Could other objective sperm analyses be helpful in the choice of the most adapted assisted procreation technique? METHODS:This study concerns 78 infertile couples with insemination failures. For each semen, 21 objective param...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/BF02765835
更新日期:1997-07-01 00:00:00
abstract:PURPOSE:To evaluate the effect of the individual physician performing embryo transfer, on clinical pregnancy rates. METHOD:Data from a total of 485 consecutive embryo transfers performed on 485 women aged 23-37 years were prospectively collected for this study. All patients underwent a standard downregulation long pro...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-006-9032-6
更新日期:2006-07-01 00:00:00
abstract:PURPOSE:To explore the effect of group culture on the developmental potential of discarded embryos in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles and establish the human embryonic stem cell lines for future research. METHORDS:Fresh discarded embryos were collected from the IVF/ICSI-ET pro...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-014-0308-y
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:Cell-free mRNAs (cfmRNAs) were quantitatively measured in human seminal plasma and its relationship with semen quality was investigated. METHODS:Herein, a prospectively, controlled investigation was performed to study seminal plasma HSPA2 and uPA cfmRNA alterations between 21 asthenozoospermic patients and 16 ...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-016-0730-4
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:The aim of this study is to evaluate which cryopreservation protocol, freezing before or after swim-up, optimizes cryopreservation outcomes in terms of motile sperm count, motility, morphology, and viability, and also to establish whether sperm viability could be assessed based on sperm motility. METHODS:Fifty...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-017-1050-z
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the feasibility of adjusted mitochondrial DNA quantification in human embryos as a biomarker for implantation potential. DESIGN:Double-blind, observational, prospective analysis of an Asian population in a single university-affiliated in vitro fertilization center. A total of 1617 embryos derived...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-019-01542-6
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:We investigated whether enzymatically fabricated hyaluronan (HA) microcapsules were feasible for use in the cryopreservation of a small number of sperm. METHODS:HA microcapsules were fabricated using a system of water-immiscible fluid under laminar flow. Three sperm were injected into a hollow HA microcapsule ...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-016-0656-x
更新日期:2016-04-01 00:00:00
abstract:PURPOSE:The importance of the surrounding ovarian stromal cells and extracellular matrix in the development and maturation of follicles has recently gained attention. An aberrant extracellular matrix has been described in ovaries of patients with polycystic ovary syndrome where a more rigid structural environment, poss...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-019-01513-x
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To evaluate the efficacy of preimplantation genetic testing (PGT) for α- and β-double thalassemia combined with aneuploidy screening using next-generation sequencing (NGS). METHODS:An NGS-based PGT protocol was performed between 2017 and 2018 for twelve couples, each of which carried both α- and β-thalassemia ...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-020-01732-7
更新日期:2020-03-01 00:00:00
abstract:PROBLEM:Platelet-activating factor (PAF) plays a significant role in fertility. Preimplantation stage embryos produce PAF (ePAF) which is required for development. PAF's mechanism of action is receptor-mediated and its presence has been reported in the developing mouse and human embryo. Exposure of preimplantation stag...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1023/b:jarg.0000043703.73207.25
更新日期:2004-08-01 00:00:00
abstract:PURPOSE:There is no consensus yet in the literature on an optimal luteinizing hormone (LH) level for human chorionic gonadotrophin (hCG) trigger timing in patients undergoing frozen-thawed embryo transfer (FET) with modified natural cycles (mNC). The objective of our study was to compare the clinical results of hCG tri...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
abstract:PURPOSE:In a retrospective study we have reviewed the data of 570 consecutive IVF cycles in which a GnRH agonist (GnRHa) was started in the early follicular phase (long protocol). Cycles were divided in groups according to estradiol levels before HMG administration: A, < 20 pg/ml; B, 20 to 50 pg/ml; C, 51 to 100 pg/ml....
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/BF02212585
更新日期:1995-10-01 00:00:00
abstract:PURPOSE:To evaluate DNA synthesis and epigenetic modification in mouse oocytes during the first cell cycle following the injection of human or hamster sperm. METHODS:Mouse oocytes following the injection of human and hamster sperm and cultured in M16 medium. RESULTS:Male and female pronucleus formation, DNA synthesis...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-010-9509-1
更新日期:2011-04-01 00:00:00
abstract:PURPOSE:A pilot study was performed to test the diagnostic value of in vitro DNA fluorescence in oocytes that failed to fertilize after IVF. Ten patients with a cleavage rate less than 20% after IVF were included. RESULTS:Uncleaved oocytes were observed by fluorescence microscopy after incubation with the DNA fluoresc...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/BF01204255
更新日期:1992-12-01 00:00:00
abstract:PURPOSE:Previous studies reported that patients with endometriosis had excess nitric oxide (NO) in the reproductive tract and poor embryo development in IVF cycles. This study aims to elucidate the effects of NO on early embryo development. METHODS:Zygotes from superovulated B6CBF1 mice were cultured to blastocysts in...
journal_title:Journal of assisted reproduction and genetics
pub_type: 杂志文章
doi:10.1007/s10815-013-0045-7
更新日期:2013-08-01 00:00:00