当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Novel transglycosylation activity of β-N-acetylglucosaminidase of Lecanicillium lecanii produced by submerged culture.

Novel transglycosylation activity of β-N-acetylglucosaminidase of Lecanicillium lecanii produced by submerged culture.

Abstract：

:N-acetylglucosaminidase produced from Lecanicillium lecanii on submerged culture displayed hydrolytic and transglycosylation activities. The highest specific activity for the enzyme was 1.87 U/mg after 120 h of culture. The chromatographic purification for a single protein fraction showed a molecular weight of 50.4 kDa and hydrolytic N-acetylglucosaminidase activity of 17.59 U/mg at 37 °C and pH 6. This enzyme was able to transglycosylate and to synthesize oligosaccharides from 2 to 6 units with a degree of acetylation between 100 and 26% employing glucose, mannose, N-acetyl-D-glucosamine and N-acetyl-D-lactosamine as donor substrates. Optimal conditions of temperature and pH were determined for both types of enzymatic activities.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Rojas-Osnaya J,Rocha-Pino Z,Nájera H,González-Márquez H,Shirai K

doi

10.1016/j.ijbiomac.2019.12.237

subject

Has Abstract

pub_date

2020-02-15 00:00:00

pages

759-767

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)37905-X

journal_volume

145

pub_type

杂志文章

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