Abstract:
:Methotrexate loaded chitin nanogel (MCNG) was formulated for its topical use in psoriasis. MCNG was characterized by DLS, TEM and FTIR. The MCNG particles were spherical in shape with size of 196±14nm, having surface charge of +9.21±0.42 mv. MCNG exhibited greater swelling and drug release at acidic pH than neutral and alkaline conditions. The treatment with MCNG showed significant level of toxicity on HaCaT and THP-1 cells and was taken up well by HaCaT cells as revealed by fluorescent microscopy. MCNGs exhibited significant anti-inflammatory activity as revealed by the inhibitory effects observed on the activity of COX-2 and LOX-5 enzymes expressed in THP-1 cells. Skin permeation studies revealed an increased trasdermal flux of methotrexate from MCNG with loosened stratum corneum and other epidermal layers of skin in comparison with control methotrexate solution treated samples. Significant anti-psoriatic efficacy on imiquimod (IMQ) induced model of psoriasis without any dermal and systemic toxicities suggest that it as an ideal delivery platform for topical delivery of methotrexate in psoriasis. Thus it is expected to become a better alternative for the oral delivery of methotrexate in the selected disease.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Panonnummal R,Sabitha Mdoi
10.1016/j.ijbiomac.2017.10.112subject
Has Abstractpub_date
2018-04-15 00:00:00pages
245-258eissn
0141-8130issn
1879-0003pii
S0141-8130(17)32969-0journal_volume
110pub_type
杂志文章abstract::A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper. Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, T...
journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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doi:10.1016/j.ijbiomac.2018.11.204
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.106
更新日期:2018-10-01 00:00:00
abstract::The PHA-degrading isolate, strain P37C, was enriched from residential compost for its ability to hydrolyze the medium chain length PHA, poly(beta-hydroxyoctanoate) (PHO). It was subsequently found to grow on a wide range of PHAs, including both short chain length and medium chain length PHAs. The isolate was identifie...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/s0141-8130(99)00027-6
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abstract::Injectable thermo-responsive hydrogels have been studied for various biomedical applications including; drug delivery, cell encapsulation, and tissue repairing. There are various natural and synthetic polymers which exhibit homogenous injectable solution at ambient temperature and form a gel in human body temperature....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2019.04.131
更新日期:2019-07-15 00:00:00
abstract::Poly-L-lysine (PLL) and bovine serum albumin were used as models in the investigation of non-enzymatic glycation and browning reaction by circular dichroism measurements. Different sugars (glucose, glucose-6-P and ribose) induced different extents of reaction and produced different chromophores that absorbed light in ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(90)90009-y
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abstract::In vitro fibrillation of hen egg white lysozyme (HEWL) causes complete reduction of Cu(II) to Cu(I) at pH 7. Here in the present article, we have shown the presence of both Cu(II) and Cu(I) at pH 11 during fibrillation of HEWL using electron paramagnetic resonance and Raman spectroscopy. Our results suggest the existe...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.02.014
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abstract::To gain a deeper understanding of the molecular basis of pollen foraging preference, we characterized the proteomes of antennae and brains of bees foraging on pear and rapeseed flowers, and the volatile compounds from nectar, anther, and inflorescence of both plants. Bees foraging on the pollen of the two plants have ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.10.140
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abstract::The purpose of the present study was to synthesize gum kondagogu-g-poly(N-vinyl-2-pyrrolidone) and to evaluate its mucoadhesive properties. UV-assisted graft co-polymerization of N-vinyl-2-pyrrolidone on gum kondagogu was carried out employing three-factor, three-level central composite experimental designs. It was ob...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.07.009
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journal_title:International journal of biological macromolecules
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abstract::Different decolorization methods have different effects on the properties of polysaccharides. In this study, polysaccharides extracted from Thesium chinense Turcz (T. chinense) were decolorized using either hydrogen peroxide (H2O2), which has been previously reported, or activated carbon. To begin, the conditions for ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.11.074
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abstract::Proteoglycan (P1) purified from Phellinus linteus has been reported to have anti-disease activities. The objectives of our research were to determine the anti-tumor effect and possible mechanisms of P1 on human cancer cells. Cell inhibition assay showed that P1 has an antiproliferative effect on HepG2, HT-29, NCI-H 46...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
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abstract::Semi-essential arginine amino acid was selected to synthesis an organosilane linker for modifying chitosan biopolymer. The novel organosilane linker which was chemically synthesized by reaction of arginine with 3‑chloropropyl trimethoxy silane, covalently bonded to the chitosan amino group. The chemical structure of r...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.145
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abstract::Hydrodynamic data, i.e. intrinsic viscosity and sedimentation coefficient, for denatured globular proteins in 6 M guanidinium chloride have been re-analysed in terms of the Yamakawa-Fujii theory of the wormlike cylinder model. Molecular parameters thus obtained ((mean value of R2 0/M) infinity, the Kuhn statistical se...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(91)90078-9
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abstract::The chemical extraction and the characterization of polysaccharides from mucilage (MC), pectin (PC) and total pectic mucilage fraction (TFC) of Opuntia ficus indica cladodes as well as the evaluation of their antioxidant activities was investigated. The FTIR spectroscopic analysis revealed the presence of carboxyl and...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.07.042
更新日期:2016-11-01 00:00:00
abstract::The effect of gamma irradiation, with doses from 10(2) to 10(3) kGy, on the dielectric relaxation time of solid-state collagen was studied. Temperature measurements of the relaxation time were made over a range of frequency of the electric field from 10(1) to 10(5) Hz and at temperatures from 298 to 480 K. The samples...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(95)93510-5
更新日期:1995-02-01 00:00:00
abstract::A new hoop shaped three dimensional chitin was obtained successfully from the body segment of a diplopod species (Ommatoiulus sabulosus) by following the procedure decolorization, demineralization and deproteinization. Purity of the hoop shaped three-dimensional chitin was proved by FT-IR analysis and chitinase digest...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.09.103
更新日期:2017-01-01 00:00:00
abstract::In this study, chitosan coatings incorporated with propolis extract (PE) were utilized to extend the shelf-life of refrigerated Nemipterus japonicus fillets. Microbiological (total mesophilic bacteria (TMB) and total psychrotrophic count (TPC)), physicochemical (pH, total volatile bases nitrogen (TVB-N), 2-thiobarbitu...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.204
更新日期:2019-10-15 00:00:00
abstract::In this work, we prepared novel cellulosic paper by incorporating tannin into the kraft pulp for potential application in active food packaging. The kraft pulp fibers were firstly periodate oxidized to obtain the dialdehyde cellulosic fibers, and then reacted with varied dosages of tannin to incorporate them into the ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.101
更新日期:2020-11-01 00:00:00
abstract::The process of recombinant protein production in E. coli system is often hampered by the formation of insoluble aggregates. Human Dihydrofolate reductase (hDHFR), an enzyme involved in the synthesis of purine, thymidilate and several other amino acids like glycine, methionine and serine is highly aggregation prone. It...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.05.143
更新日期:2017-10-01 00:00:00