Anti-psoriatic and toxicity evaluation of methotrexate loaded chitin nanogel in imiquimod induced mice model.

Abstract:

:Methotrexate loaded chitin nanogel (MCNG) was formulated for its topical use in psoriasis. MCNG was characterized by DLS, TEM and FTIR. The MCNG particles were spherical in shape with size of 196±14nm, having surface charge of +9.21±0.42 mv. MCNG exhibited greater swelling and drug release at acidic pH than neutral and alkaline conditions. The treatment with MCNG showed significant level of toxicity on HaCaT and THP-1 cells and was taken up well by HaCaT cells as revealed by fluorescent microscopy. MCNGs exhibited significant anti-inflammatory activity as revealed by the inhibitory effects observed on the activity of COX-2 and LOX-5 enzymes expressed in THP-1 cells. Skin permeation studies revealed an increased trasdermal flux of methotrexate from MCNG with loosened stratum corneum and other epidermal layers of skin in comparison with control methotrexate solution treated samples. Significant anti-psoriatic efficacy on imiquimod (IMQ) induced model of psoriasis without any dermal and systemic toxicities suggest that it as an ideal delivery platform for topical delivery of methotrexate in psoriasis. Thus it is expected to become a better alternative for the oral delivery of methotrexate in the selected disease.

journal_name

Int J Biol Macromol

authors

Panonnummal R,Sabitha M

doi

10.1016/j.ijbiomac.2017.10.112

subject

Has Abstract

pub_date

2018-04-15 00:00:00

pages

245-258

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)32969-0

journal_volume

110

pub_type

杂志文章
  • Redox-responsive blend hydrogel films based on carboxymethyl cellulose/chitosan microspheres as dual delivery carrier.

    abstract::A simple method to fabricate redox-responsive composite hydrogel films via incorporating chitosan (CS) microspheres into carboxymethyl cellulose (CMC) hydrogel using a di-sulphide cross-linker cystamine dihydrochloride (CYS) have done in this paper. Dual drugs, an anticancer, 5-Fluorouracil (5-FU) and an antibiotic, T...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.05.049

    authors: Wang F,Zhang Q,Li X,Huang K,Shao W,Yao D,Huang C

    更新日期:2019-08-01 00:00:00

  • Comparative analysis reveals the expansion of mitochondrial DNA control region containing unusually high G-C tandem repeat arrays in Nasonia vitripennis.

    abstract::Insect mitochondrial DNA (mtDNA) ranges from 14 to 19 kbp, and the size difference is attributed to the AT-rich control region. Jewel wasps have a parasitoid lifestyle, which may affect mitochondria function and evolution. We sequenced, assembled, and annotated mitochondrial genomes in Nasonia and outgroup species. Ge...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.11.007

    authors: Lin ZJ,Wang X,Wang J,Tan Y,Tang X,Werren JH,Zhang D,Wang X

    更新日期:2021-01-01 00:00:00

  • Development and characterization of bioactive edible films from spider crab (Maja crispata) chitosan incorporated with Spirulina extract.

    abstract::Active food packaging films based on crab chitosan and Spirulina extract (SE) were developed. The effects of the SE incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.07.046

    authors: Balti R,Mansour MB,Sayari N,Yacoubi L,Rabaoui L,Brodu N,Massé A

    更新日期:2017-12-01 00:00:00

  • Unravelling the mechanism of the interactions of oxazolidine A and E with collagens in ovine skin.

    abstract::Cross-linking agents play an important part in the physical properties of collagen based biomaterials. Oxazolidines are novel aldehydic tanning agents that are widely used to stabilise collagens in the leather industry. The exact mechanism through which they cross-link collagens is, however, not well understood. When ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2006.09.003

    authors: Choudhury SD,DasGupta S,Norris GE

    更新日期:2007-03-10 00:00:00

  • Azobenzene-grafted carboxymethyl cellulose hydrogels with photo-switchable, reduction-responsive and self-healing properties for a controlled drug release system.

    abstract::In this study, multifunctional hydrogels containing host-guest complex formation between azobenzene-grafted carboxymethyl cellulose (CMC-Azo) and β-cyclodextrin (CD) dimers connected by disulfide bonds with agarose for structural support were prepared. The obtained hydrogels exhibited self-healing properties by host-g...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.071

    authors: Kim Y,Jeong D,Shinde VV,Hu Y,Kim C,Jung S

    更新日期:2020-11-15 00:00:00

  • Elongation pattern and fine structure of the sheaths formed by Thiothrix nivea and Thiothrix fructosivorans.

    abstract::Thiothrix strains are filamentous sulfur-oxidizing bacteria common in activated sludge. Some of the members, including Thiothrix nivea and T. fructosivorans, are known to form a microtubular sheath that covers a line of cells. The sheaths are assemblages of [→4)-β-d-GlcN-(1→4)-β-d-Glc-(1→]n modified with unusual deoxy...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.11.025

    authors: Kawasaki Y,Endo T,Fujiwara A,Kondo K,Katahira M,Nittami T,Sato M,Takeda M

    更新日期:2017-02-01 00:00:00

  • Super and selective adsorption of cationic dyes using carboxylate-modified lignosulfonate by environmentally friendly solvent-free esterification.

    abstract::Herein, following the strategy of sustainable, environment protection, circular economy development, carboxylate-modification lignosulfonate polymer (M-LSP) was synthesized from lignosulfonate by solvent-free esterifying with maleic anhydride (MA) by one step, and was used to remove the dyes by adsorption. FT-IR and X...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.05.037

    authors: Tang Y,Lin T,Ai S,Li Y,Zhou R,Peng Y

    更新日期:2020-09-15 00:00:00

  • Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells.

    abstract::In this study, an oxidative stress model was first developed in a mouse macrophage cell line (RAW264.7 cells) by infecting the cells with porcine circovirus type 2 (PCV2). The regulatory effect of Sophora subprosrate polysaccharide (SSP) on PCV2-induced oxidative stress was investigated. The results showed that after ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2013.09.026

    authors: Su ZJ,Wei YY,Yin D,Shuai XH,Zeng Y,Hu TJ

    更新日期:2013-11-01 00:00:00

  • Susceptibilities of bacterial cellulose containing N-acetylglucosamine residues for cellulolytic and chitinolytic enzymes.

    abstract::Detailed characterization of enzyme susceptibility of bacterial cellulose containing N-acetylglucosamine (GlcNAc) residues (N-AcGBC) which possess high susceptibility for cellulase and lysozyme and slight susceptibility for chitinase was studied. Turbidimetric lysozyme assay of N-AcGBC showed that (i) the susceptibili...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/s0141-8130(05)80076-5

    authors: Ogawa R,Miura Y,Tokura S,Koriyama T

    更新日期:1992-12-01 00:00:00

  • CR-LAAO antileukemic effect against Bcr-Abl(+) cells is mediated by apoptosis and hydrogen peroxide.

    abstract::Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of the Bcr-Abl tyrosine kinase protein, which confers resistance to apoptosis in leukemic cells. Tyrosine kinase inhibitors (TKIs) are effectively used to treat CML; however, CML patients in the advanced (CML-AP) and chronic ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.01.069

    authors: Burin SM,Ghisla S,Ouchida AT,Aissa AF,Coelho MG,Costa TR,Marsola AP,Pinto-Simões B,Antunes LM,Curti C,Sampaio SV,de Castro FA

    更新日期:2016-05-01 00:00:00

  • Physicochemical characterisation of Salvia macrosiphon gum based edible films incorporated with various fatty acids.

    abstract::In the present study, the influence of different concentrations of three fatty acids (palmitic, stearic, and oleic acids) on improving the physicochemical, barrier, mechanical, thermal and surface properties of Salvia macrosiphon seed gum (SSG) edible films was investigated. The results revealed that the selected fatt...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.08.034

    authors: Amini AM,Razavi SMA

    更新日期:2020-11-01 00:00:00

  • Biodegradation of polyvinyl alcohol using cross-linked enzyme aggregates of degrading enzymes from Bacillus niacini.

    abstract::In this study, polyvinyl alcohol (PVA)-degrading bacteria were screened from sludge samples using PVA as a sole source of carbon. A novel strain was obtained and identified as Bacillus niacini based on the analysis of a partial 16S rDNA nucleotide sequence and morphological characteristics. PVA-degrading enzyme (PVAas...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.11.204

    authors: Bian H,Cao M,Wen H,Tan Z,Jia S,Cui J

    更新日期:2019-03-01 00:00:00

  • Synthesis and characterization of cross linked enzyme aggregates of serine hydroxyl methyltransferase from Idiomerina leihiensis.

    abstract::A thermo-stable purified serine hydroxymethyltransferase (SHMT; 418 AA) was used for the carrier free immobilization using pectin as a coach molecule and formaldehyde as a cross-linker. The purified protein was cross linked with formaldehyde in the presence of pectin to form stable and active aggregates. The cross-lin...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.04.106

    authors: Kumar A,Wu G,Liu Z

    更新日期:2018-10-01 00:00:00

  • Extracellular degradation of medium chain length poly(beta-hydroxyalkanoates) by Comamonas sp.

    abstract::The PHA-degrading isolate, strain P37C, was enriched from residential compost for its ability to hydrolyze the medium chain length PHA, poly(beta-hydroxyoctanoate) (PHO). It was subsequently found to grow on a wide range of PHAs, including both short chain length and medium chain length PHAs. The isolate was identifie...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/s0141-8130(99)00027-6

    authors: Quinteros R,Goodwin S,Lenz RW,Park WH

    更新日期:1999-06-01 00:00:00

  • Polysaccharide and polypeptide based injectable thermo-sensitive hydrogels for local biomedical applications.

    abstract::Injectable thermo-responsive hydrogels have been studied for various biomedical applications including; drug delivery, cell encapsulation, and tissue repairing. There are various natural and synthetic polymers which exhibit homogenous injectable solution at ambient temperature and form a gel in human body temperature....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2019.04.131

    authors: Darge HF,Andrgie AT,Tsai HC,Lai JY

    更新日期:2019-07-15 00:00:00

  • Circular dichroism of the non-enzymatic browning products of poly-L-lysine and albumin.

    abstract::Poly-L-lysine (PLL) and bovine serum albumin were used as models in the investigation of non-enzymatic glycation and browning reaction by circular dichroism measurements. Different sugars (glucose, glucose-6-P and ribose) induced different extents of reaction and produced different chromophores that absorbed light in ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/0141-8130(90)90009-y

    authors: Liang JN

    更新日期:1990-08-01 00:00:00

  • Evidence of two oxidation states of copper during aggregation of hen egg white lysozyme (HEWL).

    abstract::In vitro fibrillation of hen egg white lysozyme (HEWL) causes complete reduction of Cu(II) to Cu(I) at pH 7. Here in the present article, we have shown the presence of both Cu(II) and Cu(I) at pH 11 during fibrillation of HEWL using electron paramagnetic resonance and Raman spectroscopy. Our results suggest the existe...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.02.014

    authors: Ghosh S,Pandey NK,Bhattacharya S,Roy A,Nagy NV,Dasgupta S

    更新日期:2015-05-01 00:00:00

  • Proteome analysis reveals a strong correlation between olfaction and pollen foraging preference in honeybees.

    abstract::To gain a deeper understanding of the molecular basis of pollen foraging preference, we characterized the proteomes of antennae and brains of bees foraging on pear and rapeseed flowers, and the volatile compounds from nectar, anther, and inflorescence of both plants. Bees foraging on the pollen of the two plants have ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.10.140

    authors: Guo Y,Fu B,Qin G,Song H,Wu W,Shao Y,Altaye SZ,Yu L

    更新日期:2019-01-01 00:00:00

  • Synthesis of gum kondagogu-g-poly(N-vinyl-2-pyrrolidone) and its evaluation as a mucoadhesive polymer.

    abstract::The purpose of the present study was to synthesize gum kondagogu-g-poly(N-vinyl-2-pyrrolidone) and to evaluate its mucoadhesive properties. UV-assisted graft co-polymerization of N-vinyl-2-pyrrolidone on gum kondagogu was carried out employing three-factor, three-level central composite experimental designs. It was ob...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2012.07.009

    authors: Malik S,Kumar A,Ahuja M

    更新日期:2012-12-01 00:00:00

  • Glycation of α-synuclein amplifies the binding with glyceraldehyde-3-phosphate dehydrogenase.

    abstract::α-Synuclein was recently found to interact with moonlighting glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) involved in neurodegenerative diseases development. In the present work, we have analyzed influence of α-synuclein glycation on this interaction, because the literature data suggest relation ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.01.064

    authors: Semenyuk P,Barinova K,Muronetz V

    更新日期:2019-04-15 00:00:00

  • Decolorization affects the structural characteristics and antioxidant activity of polysaccharides from Thesium chinense Turcz: Comparison of activated carbon and hydrogen peroxide decolorization.

    abstract::Different decolorization methods have different effects on the properties of polysaccharides. In this study, polysaccharides extracted from Thesium chinense Turcz (T. chinense) were decolorized using either hydrogen peroxide (H2O2), which has been previously reported, or activated carbon. To begin, the conditions for ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.11.074

    authors: Shao L,Sun Y,Liang J,Li M,Li X

    更新日期:2020-07-15 00:00:00

  • Anti-tumor effects of proteoglycan from Phellinus linteus by immunomodulating and inhibiting Reg IV/EGFR/Akt signaling pathway in colorectal carcinoma.

    abstract::Proteoglycan (P1) purified from Phellinus linteus has been reported to have anti-disease activities. The objectives of our research were to determine the anti-tumor effect and possible mechanisms of P1 on human cancer cells. Cell inhibition assay showed that P1 has an antiproliferative effect on HepG2, HT-29, NCI-H 46...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2011.01.014

    authors: Li YG,Ji DF,Zhong S,Zhu JX,Chen S,Hu GY

    更新日期:2011-04-01 00:00:00

  • Synthesis and characterization of a novel organosilane-functionalized chitosan nanocarrier as an efficient gene delivery system: Expression of green fluorescent protein.

    abstract::Semi-essential arginine amino acid was selected to synthesis an organosilane linker for modifying chitosan biopolymer. The novel organosilane linker which was chemically synthesized by reaction of arginine with 3‑chloropropyl trimethoxy silane, covalently bonded to the chitosan amino group. The chemical structure of r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.11.145

    authors: Kashkouli KI,Torkzadeh-Mahani M,Mosaddegh E

    更新日期:2019-03-15 00:00:00

  • Analysis of hydrodynamic data for denatured globular proteins in terms of the wormlike cylinder model.

    abstract::Hydrodynamic data, i.e. intrinsic viscosity and sedimentation coefficient, for denatured globular proteins in 6 M guanidinium chloride have been re-analysed in terms of the Yamakawa-Fujii theory of the wormlike cylinder model. Molecular parameters thus obtained ((mean value of R2 0/M) infinity, the Kuhn statistical se...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/0141-8130(91)90078-9

    authors: Bohdanecký M,Petrus V

    更新日期:1991-08-01 00:00:00

  • Extraction and characterization of three polysaccharides extracted from Opuntia ficus indica cladodes.

    abstract::The chemical extraction and the characterization of polysaccharides from mucilage (MC), pectin (PC) and total pectic mucilage fraction (TFC) of Opuntia ficus indica cladodes as well as the evaluation of their antioxidant activities was investigated. The FTIR spectroscopic analysis revealed the presence of carboxyl and...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.07.042

    authors: Bayar N,Kriaa M,Kammoun R

    更新日期:2016-11-01 00:00:00

  • Temperature variation of the relaxation time of alpha-dispersion for gamma-irradiated collagen.

    abstract::The effect of gamma irradiation, with doses from 10(2) to 10(3) kGy, on the dielectric relaxation time of solid-state collagen was studied. Temperature measurements of the relaxation time were made over a range of frequency of the electric field from 10(1) to 10(5) Hz and at temperatures from 298 to 480 K. The samples...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/0141-8130(95)93510-5

    authors: Marzec E

    更新日期:1995-02-01 00:00:00

  • Chitin hoops obtained from Ommatoiulus sabulosus (Diplopoda, Julidae) used for bovine serum albumin adsorption.

    abstract::A new hoop shaped three dimensional chitin was obtained successfully from the body segment of a diplopod species (Ommatoiulus sabulosus) by following the procedure decolorization, demineralization and deproteinization. Purity of the hoop shaped three-dimensional chitin was proved by FT-IR analysis and chitinase digest...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.09.103

    authors: Šatkauskienė I,Jarusevičiūtė S,Baublys V,Maheta M,Tubelytė V

    更新日期:2017-01-01 00:00:00

  • The shelf life extension of refrigerated Nemipterus japonicus fillets by chitosan coating incorporated with propolis extract.

    abstract::In this study, chitosan coatings incorporated with propolis extract (PE) were utilized to extend the shelf-life of refrigerated Nemipterus japonicus fillets. Microbiological (total mesophilic bacteria (TMB) and total psychrotrophic count (TPC)), physicochemical (pH, total volatile bases nitrogen (TVB-N), 2-thiobarbitu...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.07.204

    authors: Ebadi Z,Khodanazary A,Hosseini SM,Zanguee N

    更新日期:2019-10-15 00:00:00

  • Cellulosic paper with high antioxidative and barrier properties obtained through incorporation of tannin into kraft pulp fibers.

    abstract::In this work, we prepared novel cellulosic paper by incorporating tannin into the kraft pulp for potential application in active food packaging. The kraft pulp fibers were firstly periodate oxidized to obtain the dialdehyde cellulosic fibers, and then reacted with varied dosages of tannin to incorporate them into the ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.06.101

    authors: Ji Y,Xu Q,Jin L,Fu Y

    更新日期:2020-11-01 00:00:00

  • Osmolyte induced enhancement of expression and solubility of human dihydrofolate reductase: An in vivo study.

    abstract::The process of recombinant protein production in E. coli system is often hampered by the formation of insoluble aggregates. Human Dihydrofolate reductase (hDHFR), an enzyme involved in the synthesis of purine, thymidilate and several other amino acids like glycine, methionine and serine is highly aggregation prone. It...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.05.143

    authors: Rashid N,Thapliyal C,Chaudhuri Chattopadhyay P

    更新日期:2017-10-01 00:00:00