Transplantation of iPS-derived vascular endothelial cells improves white matter ischemic damage.


:White matter infarct induces demyelination and brain dysfunction. We previously reported that transplantation of brain microvascular endothelial cells improved the behavioral outcome and promoted remyelination by increasing the number of oligodendrocyte precursor cells in the rat model of white matter infarct. In this study, we investigated the effects of transplantation of vascular endothelial cells generated from human induced pluripotent stem cells (iPSCs) on the rat model of white matter infarct. Seven days after induction of ischemic demyelinating lesion by injection of endothelin-1 into the internal capsule of a rat brain, iPSC-derived vascular endothelial cells (iVECs) were transplanted into the site of demyelination. The majority of iVECs transplanted into the internal capsule survived for 14 days after transplantation when traced by immunohistochemistry for a human cytoplasmic protein. iVEC transplantation significantly recovered hind limb rotation angle as compared to human iPSC or rat meningeal cell transplantation when evaluated using footprint test. Fourteen days after iVEC transplantation, the infarct area remarkably decreased as compared to that just before the transplantation when evaluated using magnetic resonance imaging or luxol fast blue staining, and remyelination was promoted dramatically in the infarct when assessed using luxol fast blue staining. Transplantation of iVECs increased the number of oligodendrocyte lineage cells and suppressed the inflammatory response and reactive astrocytogenesis. These results suggest that iVEC transplantation may prove useful in treatment for white matter infarct.


J Neurochem


Xu B,Kurachi M,Shimauchi-Ohtaki H,Yoshimoto Y,Ishizaki Y




Has Abstract


2020-06-01 00:00:00












  • Irreversible inhibition of calcium uptake in synaptosomes by quinacrine mustard: relationship to labeling sites of quinacrine mustard.

    abstract::The sites of interaction of quinacrine with synaptic membranes were labeled with quinacrine mustard. Quinacrine mustard had an inhibitory effect on depolarization-induced calcium uptake by synaptosomes similar to that of quinacrine. The inhibition of depolarization-induced calcium uptake by quinacrine was reduced by 7...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ohta A,Baba A,Iwata H

    更新日期:1985-08-01 00:00:00

  • [3H]muscimol binding site on purified benzodiazepine receptor.

    abstract::The presence of a [3H]muscimol binding site on the purified benzodiazepine receptor was demonstrated. The purified protein was apparently homogeneous as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis (stained with silver), with a molecular weight of 60,000 +/- 3000. The benzodiazepine binding sites...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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  • Time-course of mitochondrial gene expressions in mice brains: implications for mitochondrial dysfunction, oxidative damage, and cytochrome c in aging.

    abstract::The study of aging is critical for a better understanding of many age-related diseases. The free radical theory of aging, one of the prominent aging hypotheses, holds that during aging, increasing reactive oxygen species in mitochondria causes mutations in the mitochondrial DNA and damages mitochondrial components, re...

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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  • Mass fragmentographic method for the determination of trace amounts of putative amino acid neurotransmitters and related compounds from brain perfusates collected in vivo.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Meng F,Tsou K

    更新日期:1988-05-01 00:00:00

  • Nitrite and nitrate levels in individual molluscan neurons: single-cell capillary electrophoresis analysis.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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  • Synthesis and translocation of gangliosides and glycoproteins during urethane anesthesia.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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  • Spermidine synthase is prominently expressed in the striatal patch compartment and in putative interneurones of the matrix compartment.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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    更新日期:2006-04-01 00:00:00