Abstract:
:Solving the global environmental and agricultural problem of chronic low-level cadmium (Cd) exposure requires better mechanistic understanding. Here, soybean (Glycine max) plants were exposed to Cd concentrations ranging from 0.5 nM (background concentration, control) to 3 µM. Plants were cultivated hydroponically under non-nodulating conditions for 10 weeks. Toxicity symptoms, net photosynthetic oxygen production and photosynthesis biophysics (chlorophyll fluorescence: Kautsky and OJIP) were measured in young mature leaves. Cd binding to proteins [metalloproteomics by HPLC-inductively coupled plasma (ICP)-MS] and Cd ligands in light-harvesting complex II (LHCII) [X-ray absorption near edge structure (XANES)], and accumulation of elements, chloropyll, and metabolites were determined in leaves after harvest. A distinct threshold concentration of toxicity onset (140 nM) was apparent in strongly decreased growth, the switch-like pattern for nutrient uptake and metal accumulation, and photosynthetic fluorescence parameters such as Φ RE10 (OJIP) and saturation of the net photosynthetic oxygen release rate. XANES analyses of isolated LHCII revealed that Cd was bound to nitrogen or oxygen (and not sulfur) atoms. Nutrient deficiencies caused by inhibited uptake could be due to transporter blockage by Cd ions. The changes in specific fluorescence kinetic parameters indicate electrons not being transferred from PSII to PSI. Inhibition of photosynthesis combined with inhibition of root function could explain why amino acid and carbohydrate metabolism decreased in favour of molecules involved in Cd stress tolerance (e.g. antioxidative system and detoxifying ligands).
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Andresen E,Lyubenova L,Hubáček T,Bokhari SNH,Matoušková Š,Mijovilovich A,Rohovec J,Küpper Hdoi
10.1093/jxb/erz530subject
Has Abstractpub_date
2020-02-19 00:00:00pages
1628-1644issue
4eissn
0022-0957issn
1460-2431pii
5639660journal_volume
71pub_type
杂志文章abstract::Heterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the G alpha subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated t...
journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp135
更新日期:2009-01-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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更新日期:2013-03-01 00:00:00
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/err241
更新日期:2012-01-01 00:00:00
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jexbot/53.370.979
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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更新日期:2014-11-01 00:00:00
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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更新日期:2017-06-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of experimental botany
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of experimental botany
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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更新日期:2006-01-01 00:00:00