Production and faecal fermentation of pentose oligomers of hemicellulose: Study of variables influencing bioprocess efficiency.

Abstract:

:Almond shell, a by-product obtained from the nut industry, was valorised into low degree of polymerisation xylooligosaccharides using alkaline pretreatment and enzymatic hydrolysis. The effect of particle size on hemicellulose recovery upon pretreatment was studied using 1 and 2 M NaOH. It was observed that particle size significantly influences hemicellulose recovery, as particles below 120 µm resulted in near complete recovery at 2 M NaOH. Enzymatic hydrolysis of hemicellulose was optimised using response surface methodology, to obtain efficient xylooligosaccharides production at low enzyme dose and high substrate concentration. For higher XOS yield, an enzyme dose of 10 U and substrate concentration <2% was optimal. The in-vitro human faecal fermentation study revealed no significant difference in gas and short chain fatty acid level among substrates evaluated. It was observed that short chain oligosaccharides produce higher level of acetate than medium chain oligosaccharides.

journal_name

Food Chem

journal_title

Food chemistry

authors

Singh RD,So D,Yao CK,Gill P,Pillai N,Muir J,Arora A

doi

10.1016/j.foodchem.2019.06.012

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

124945

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(19)31041-6

journal_volume

297

pub_type

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