Abstract:
:Selective inhibition (SI) has been routinely used to differentiate the contributions of bacteria and fungi to soil ecological processes. SI experiments typically measured rapid responses within hours since the addition of inhibitor, but the long-term effects of selective biocides on microbial community composition and function were largely unknown. In this study, a microcosm experiment was performed with an agricultural soil to explore the effectiveness of two bactericides (bronopol, streptomycin) and two fungicides (cycloheximide, captan), which were applied at two different concentrations (2 and 10 mg g-1). The microcosms were incubated for 6 weeks. A radiolabeled substrate, [1,2,3,4,4a,9a-14C] anthracene, was spiked to all microcosms, and the derived CO2 was monitored during the incubation. The abundance and composition of bacteria and fungi were assessed by qPCR and Miseq sequencing of ribosomal rRNA genes. It was demonstrated that only 2 mg g-1 bronopol and cycloheximide significantly changed the bacteria to fungi ratio without apparent non-target inhibition on the abundances; however, community shifts were observed in all treatments after 6 weeks incubation. The enrichment of specific taxa implicated a selection of resistant or adapted microbes by these biocides. Mineralization of anthracene was continuingly suppressed in all SI microcosms, which may result in biased estimate of bacterial and fungal contributions to pollutant degradation. These findings highlight the risks of long-term application of selective inhibition, and a preliminary assessment of biocide selection and concentration is highly recommended.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Pan Y,Wu Y,Li X,Zeng J,Lin Xdoi
10.1007/s00248-019-01364-0subject
Has Abstractpub_date
2019-11-01 00:00:00pages
927-935issue
4eissn
0095-3628issn
1432-184Xpii
10.1007/s00248-019-01364-0journal_volume
78pub_type
杂志文章abstract::Although phototrophic microbial communities are important components of soils in arid and semi-arid ecosystems around the world, the knowledge of their taxonomic composition and dependency on soil chemistry and vegetation is still fragmentary. We studied the abundance and the diversity of cyanobacteria and eukaryotic ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-011-9878-8
更新日期:2011-08-01 00:00:00
abstract::Rice sheath rot has been mainly associated with the bacterial pathogen Pseudomonas fuscovaginae and in some cases to the fungal pathogen Sarocladium oryzae; it is yet unclear if they are part of a complex disease. The bacterial and fungal community associated with rice sheath rot symptomatic and asymptomatic rice plan...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-020-01529-2
更新日期:2020-10-01 00:00:00
abstract::The marine, free-living Stilbonematinae (Nematoda: Desmodorida) inhabit the oxygen sulfide chemocline in marine sands. They are characterized by an association with ectosymbiotic bacteria. According to their ultrastructure the bacteria are Gram-negative and form morphologically uniform coats that cover the entire body...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF00167789
更新日期:1992-11-01 00:00:00
abstract::Staphylococcus aureus, an opportunistic pathogen member of the nasal and skin microbiota, can also be found in human oral samples and has been linked to infectious diseases of the oral cavity. As the nasal and oral cavities are anatomically connected, it is currently unclear whether S. aureus can colonize the oral cav...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-018-1291-0
更新日期:2019-08-01 00:00:00
abstract::The symbiotic microbes that grow in and on many organisms can play important roles in protecting their hosts from pathogen infection. While species diversity has been shown to influence community function in many other natural systems, the question of how species diversity of host-associated symbiotic microbes contrib...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-016-0916-4
更新日期:2017-07-01 00:00:00
abstract::Propionate is one of the major intermediates in anaerobic digestion of organic waste to CO2 and CH4. In methanogenic environments, propionate is degraded through a mutualistic interaction between symbiotic propionate oxidizers and methanogens. Although temperature heavily influences the microbial ecology and performan...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-020-01485-x
更新日期:2020-07-01 00:00:00
abstract::One of the major challenges in microbial ecology for the future is to establish links between structural and functional biodiversity. This is particularly difficult when one is interested in a phylogenetically diversified function such as denitrification. The data banks are very rich in functional gene sequences (nirS...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-004-0256-7
更新日期:2005-02-01 00:00:00
abstract::Scleractinian corals form the framework of coral reefs and host abundant and diverse microbial communities that are fundamental to their success. A very limited number of studies have examined the co-occurrence of multiple partners within the coral 'holobiont' and their pattern of specificity over different geographic...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-018-1255-4
更新日期:2019-04-01 00:00:00
abstract::Viable bacteria were found in permafrost core samples from the Kolyma-Indigirka lowland of northeast Siberia. The samples were obtained at different depths; the deepest was about 3 million years old. The average temperature of the permafrost is -10 degrees C. Twenty-nine bacterial isolates were characterized by 16S rD...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s002489900019
更新日期:1997-05-01 00:00:00
abstract::We investigated the impact of fish farm biodeposition on benthic bacteria, thraustochytrid protists, and heterotrophic protozoa (nanoflagellates and ciliates) in an oligotrophic area of the Mediterranean Sea. The fish farm impact was investigated both on a seagrass (Posidonia oceanica) bed and on soft bottom sediments...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-004-0117-4
更新日期:2005-08-01 00:00:00
abstract::Bacterial symbionts are increasingly recognised as mediators of ecologically important traits of their animal hosts, with acquisition of new traits possible by uptake of novel symbionts. The entomopathogenic nematode Heterorhabditis downesi associates with two bacterial symbionts, Photorhabdus temperata subsp. tempera...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-016-0829-2
更新日期:2017-01-01 00:00:00
abstract::Assimilatory nitrate reductase gene fragments were isolated from epiphytes and plankton associated with seagrass blades collected from Tampa Bay, Florida, USA. Nitrate reductase genes from diatoms (NR) and heterotrophic bacteria (nasA) were amplified by polymerase chain reaction (PCR) using two sets of degenerate prim...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-006-9175-0
更新日期:2007-11-01 00:00:00
abstract::In recent years, several episodes of mass mortality of sessile epibenthic invertebrates, including sponges, have been recorded worldwide. In the present study, we report a disease event on Ircinia variabilis recorded in September 2009 along the southern Adriatic and Ionian seas (Apulian coast), with the aim to quantif...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-012-0068-0
更新日期:2012-10-01 00:00:00
abstract::To elucidate the degree of microdiversity within the genus Prochlorococcus, novel Prochlorococcus-specific polymerase chain reaction (PCR) primers were developed for the rpoC1 gene, which encodes the ribonucleic acid (RNA) polymerase core subunit. The size of the PCR fragment (925 bp) coupled with high sequence variat...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-007-9259-5
更新日期:2008-01-01 00:00:00
abstract::The main current methods for controlling American Foulbrood (AFB) in honeybees, caused by the bacterial pathogen Paenibacillus larvae, are enforced incineration or prophylactic antibiotic treatment, neither of which is fully satisfactory. This has led to an increased interest in the natural relationships between the p...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-019-01434-3
更新日期:2020-04-01 00:00:00
abstract::Aeromonas species were recovered from over 27% of 183 chlorinated drinking water samples collected during an 18-month period. Sixteen of 20 isolates tested elicited a cytotoxic response by Y-1 mouse adrenal cells. None of the strains was either enterotoxigenic by the rabbit ligated ileal loop assay, exhibited piliatio...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02010672
更新日期:1982-12-01 00:00:00
abstract::Selective isolation, molecular identification and AFLP were used to investigate the distribution of the various species of endophytic and epiphytic Trichoderma associated with banana roots and to compare and contrast their genetic structure. Three specific groups of Trichoderma were observed in the roots of banana. Gr...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-010-9770-y
更新日期:2011-04-01 00:00:00
abstract::Lichens host highly diverse microbial communities, with bacteria being one of the most explored groups in terms of their diversity and functioning. These bacteria could partly originate from symbiotic propagules developed by many lichens and, perhaps more commonly and depending on environmental conditions, from differ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-020-01662-y
更新日期:2021-01-06 00:00:00
abstract::Rice root-associated microbial community play an important role in plant nutrient acquisition, biomass production, and stress tolerance. Herein, root-associated community assembly was investigated under different phosphate input levels in phosphorus (P)-deficient paddy soil. Rice was grown in a long-term P-depleted pa...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-019-01407-6
更新日期:2020-02-01 00:00:00
abstract::Interactions between bacteria and protozoa in soil were studied over 2-week periods in the field and in a pot experiment. Under natural conditions the total biological activity was temporarily synchronized by a large rainfall, and in the laboratory by the addition of water to dried-out soil, with or without plants. In...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/BF02341429
更新日期:1981-12-01 00:00:00
abstract::Saltmarshes are typically dominated by perennial grasses with large underground rhizome systems that can change local sediment conditions and be important in shaping the sediment microbial community. Factors such as salinity that control plant zonation in saltmarshes are also likely to influence the microbial communit...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-015-0651-2
更新日期:2016-02-01 00:00:00
abstract::Foliar chemistry influences leaf decomposition, but little is known about how litter chemistry affects the assemblage of bacterial communities during decomposition. Here we examined relationships between initial litter chemistry and the composition of the bacterial community in a stream ecosystem. We incubated replica...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-016-0735-7
更新日期:2016-05-01 00:00:00
abstract::Two annual Baltic Sea phytoplankton blooms occur in spring and summer. The bloom intensity is determined by nutrient concentrations in the water, while the period depends on weather conditions. During the course of the bloom, dead cells sink to the sediment where their degradation consumes oxygen to create hypoxic zon...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-018-1229-6
更新日期:2019-02-01 00:00:00
abstract::Animal manure from modern animal agriculture constitutes the single largest source of antibiotic resistance (AR) owing to the use of large quantities of antibiotics. After animal manure enters the environment, the AR disseminates into the environment and can pose a potentially serious threat to the health and well-bei...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-010-9634-5
更新日期:2010-10-01 00:00:00
abstract::Large areas of rainforest in Asia have been converted to plantations, with uncertain effects on soil biodiversity. Using standard metagenetic methods, we compared the soil biota of bacteria, fungi, and nematodes at three rainforest sites in Malaysia with two rubber plantation sites with similar soils and geology. We p...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-016-0790-0
更新日期:2016-08-01 00:00:00
abstract::Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants of the environment. But is their microbial degradation equally wide in distribution? We estimated the PAH degradation capacity of 13 soils ranging from pristine locations (total PAHs approximately 0.1 mg kg(-1)) to heavily polluted industrial sites (tot...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-005-0022-5
更新日期:2005-11-01 00:00:00
abstract::Conjugal transfer of a derivative of the RP4 plasmid between Pseudomonas fluorescens AS12 and Serratia plymuthica RF7 was compared in the rhizosphere of pea, wheat, and barley and related to the metabolic activity of the bacteria. To obtain a reliable measure of transfer, which allowed comparison of results between ex...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-001-0001-4
更新日期:2001-10-01 00:00:00
abstract::A growing body of evidence now supports the isolation of microorganisms from ancient materials. However, questions about the stringency of extraction methods and the genetic relatedness of isolated organisms to their closest living relatives continue to challenge the authenticity of these ancient life forms. Previous ...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-003-2016-5
更新日期:2004-07-01 00:00:00
abstract::Bacterioplankton community composition was studied in 12 lakes in three different geographic regions in Scandinavia using denaturing gradient gel electrophoresis (DGGE) and sequencing of 16S rDNA. Area-specific abundant taxa were found in the lakes in two of the regions. In the region of Uppland the lakes had an alpha...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-002-0007-6
更新日期:2002-07-01 00:00:00
abstract::The genus Prochlorococcus is globally abundant and dominates the total phytoplankton biomass and production in the oligotrophic ocean. The single species, Prochlorococcus marinus, comprises six named ecotypes. Our aim was to analyze the taxonomic structure of the genus Prochlorococcus. We analyzed the complete genomes...
journal_title:Microbial ecology
pub_type: 杂志文章
doi:10.1007/s00248-013-0270-8
更新日期:2013-11-01 00:00:00