Abstract：
:The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90 (Hsp90) without additional co-chaperone supplementation; intended for high-throughput screening of anti-cancer drugs and prognosis of stress. In this regard, Hsp90 and adenosine 5'-triphosphate (ATP) mediated firefly luciferase (FLuc) kinetics was investigated using apyrase and FLuc as client proteins. Bioluminescent assay containing Hsp90, ATP, and apyrase led to complete loss of luminescence at 50 °C which indicates the protective role of Hsp90 against thermal denaturation. Similarly, the assay sample comprising Hsp90, ATP, and FLuc showed 2 fold increments in luminescence than their counterparts. Introduction of bovine serum albumin (BSA) to the pre-incubated assay mixture led to an initial rise in the luminescence (28%) in comparison to the sample containing Hsp90, ATP and FLuc. Therefore, FLuc based HTS assays are not suitable for clinical samples which may contain stabilizing agents. However, thermally denatured FLuc and apyrase could not regain their active conformation even when Hsp90 and ATP were introduced in the assay system. This observation justifies the role of Hsp90 to be protective rather than a reparation agent when acts without co-chaperones.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kirillova MA,Ranjan R,Esimbekova EN,Kratasyuk VAdoi
10.1016/j.ijbiomac.2019.03.110subject
Has Abstractpub_date
2019-06-15 00:00:00pages
691-696eissn
0141-8130issn
1879-0003pii
S0141-8130(19)30763-9journal_volume
131pub_type
杂志文章abstract：:This work concerned the effects of amylose/amylopectin ratio on the structure and thermal stability of propionylated starches with high degree of substitution (DS). Four starches with different amylose content were used to obtain propionylated starches. Acylation partly disrupted granule morphology of native starches,...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.033
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abstract：:Herein, following the strategy of sustainable, environment protection, circular economy development, carboxylate-modification lignosulfonate polymer (M-LSP) was synthesized from lignosulfonate by solvent-free esterifying with maleic anhydride (MA) by one step, and was used to remove the dyes by adsorption. FT-IR and X...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:Contractile filaments in skeletal muscle are moved by less than 2nm for each ATP used. If just one cross-bridge is attached to each thin filament at any instant then this distance represents the fundamental myosin cross-bridge step size (i.e. the distance one cross-bridge moves a thin filament in one ATP-splitting cyc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:The most common cause of deaths due to cancers nowadays is lung cancer. The objective of this study was to prepare erlotinib loaded chitosan nanoparticles for their anticancer potential. To study the effect of formulation variables on prepared nanoparticles using central composite design. Erlotinib loaded chitosan nan...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:Prephenate dehydrogenase (PDH) is a bacterial enzyme that catalyzes conversion of prephenate to 4-hydroxyphenylpyruvate through the oxidative decarboxylation pathway for tyrosine biosynthesis. This enzymatic pathway exists in prokaryotes but is absent in mammals, indicating that it is a potential target for the develo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2011.07.009
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abstract：:An L-fucose lectin, ANL from the corneal smears of a mycotic keratitis patient was reported earlier. Interaction of ANL with immortalized Human Corneal Epithelial Cells (HCECs) was studied in order to assign the role of ANL in pathogenesis. ANL showed strong binding to HCECs which could be blocked by L-fucose and muci...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:Sodium houttuyfonate analogs (SHAs), CH(3)-(CH(2))(n)-CO-CH(2)-CH(OH)SO(3)Na, (n=6-14) were synthesized and their molecular interactions with renin and angiotensin I converting enzyme (ACE) studied using fluorescence quenching techniques. Unlike renin, inhibition of ACE activity was not directly proportional to the al...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2007.03.004
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(98)00065-8
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-11-15 00:00:00
abstract：:In this study, the preparation of magnetic Fe and Mn oxides (Mag-FMBO) loaded carbon aerogels (CA) based on konjac glucomannan (KGM) and their performance for dyes adsorption were investigated. The prepared magnetic carbon aerogels (Mag-CA) materials were characterized by various methods, including BET surface area an...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.07.106
更新日期：2016-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.006
更新日期：2020-12-01 00:00:00
abstract：:The interaction between biomacromolecules and ligands has attracted great interest because of their biological properties. Calf thymus DNA (ctDNA) can interact with bioactive compounds to form complexes. Here, ctDNA-ligand complexes were studied using fluorescence, absorption, and infrared spectroscopy, circular dichr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.135
更新日期：2021-01-31 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.066
更新日期：2020-10-15 00:00:00
abstract：:In this study, a MT(Al)/calcium alginate [MT(Al)@CA] microsphere structure was prepared using sodium alginate (SA) and MT(Al). In order to achieve [MT(Al)@CA] microspheres with a high stability and chemical resistance, glutaraldehyde was used as the crosslinking agent to graft the microspheres and ethylenediamine (ED)...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.172
更新日期：2019-06-15 00:00:00
abstract：:The aim of this study was to optimize the culture conditions for production, recovery and characterization of polyhydroxyalkanoate (PHA) from potential Bacillus cereus strain BNPI-92. Cost effective carbon sources such as crude oil (CO), rice bran (RB), sugar cane molasses (SCM) and wheat bran (WB) were evaluated for ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.138
更新日期：2020-08-01 00:00:00
abstract：:Here, we investigated the effect of preparation temperature and alginate-coating on L929 fibroblast behavior on lyophilized microporous PLLA/PLGA (95:5, w/w) scaffolds. The lower freezing temperature used during lyophilization (-80 °C) resulted in smaller pores (around 50 μm) and higher compressive modulus (1500 kPa) ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.169
更新日期：2019-03-01 00:00:00
abstract：:Alginate nanocomposite films incorporating sepiolite (Sep) modified with myrtle berries extract (MBE) rich in polyphenols were prepared by solution casting method. The effects of different extract concentrations on the film properties were determined by measuring physicochemical, mechanical and antioxidant properties ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.052
更新日期：2020-12-15 00:00:00
abstract：:No curative treatment is yet available for rheumatoid arthritis (RA), wherein chronic synovitis progresses to cartilage and bone destruction. Considering the recently recognized anti-inflammatory properties of Lycium barbarum polysaccharide (LBP; a derivative of the goji berry), we established the collagen type II-ind...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.04.025
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abstract：:Dextrin and poly (N-vinyl caprolactam) based amphiphilic graft copolymer has recently been developed using RAFT polymerization. The prepared graft copolymer has been characterized in details using FTIR and 1H NMR spectral analyses, GPC, TGA, FESEM, TEM and DLS analyses. GPC analysis results indicate that the polymeriz...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:The structural complexity of lignins necessitates characterization and isolation methodologies for assessing their appropriateness for thermo-chemical systems and material applications. Lignins prepared via two pulping methods (organosolv and soda) were comprehensively investigated by analyzing the properties, includi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-04-29 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.167
更新日期：2020-12-15 00:00:00
abstract：:Effects of sodium dodecyl sulfate, dodecyltrimethylammonium bromide, sodium chloride, sodium sulfate, methanol and ethanol, on the structure and activity of adenosine deaminase (ADA) were investigated by UV-Vis, circular dichroism spectrophotometry and molecular dynamics (MDs) studies. Relative activity, experimental ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2008.04.007
更新日期：2008-08-15 00:00:00
abstract：BACKGROUND:Advanced glycation end products (AGEs) have shown to possess antigenicity. This study analyzes the detrimental effect of non-enzymatic glycation on human serum albumin (HSA) leading to the production of antibodies. METHODS:HSA (20 μM) incubated with d-glucose formed AGEs confirmed by scanning electron micro...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.07.033
更新日期：2018-10-15 00:00:00
abstract：:The neurotoxicity of HIV-1 Tat protein contributes significantly to the pathogenesis of HAND, and hence the attractive therapeutic strategies focusing on Tat-induced neurotoxicity are warranted. Salvia miltiorrhiza have been known to antioxidant property and neuroprotective effects. The Danshen granule is the pharmace...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.146
更新日期：2019-03-01 00:00:00
abstract：:To fight oxidative damage due to reactive oxygen species (ROS), cells are equipped of different enzymes, among which Peroxiredoxins (Prxs) (EC 1.11.1.15) play a key role. Prxs are thiol-based enzymes containing one (1-Cys Prx) or two (2-Cys Prx) catalytic cysteine residues. In 2-Cys Prxs the cysteine residues form a d...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.052
更新日期：2020-06-15 00:00:00
abstract：:Glycol chitosan/fucoidan nanogels loaded with anti-inflammatory peptide KAFAK (GC/Fu@KAFAK NGs) were fabricated based on the electrostatic interaction and genipin cross-linking methods. The prepared NGs had an average size of 286.3 ± 5.0 nm and positive surface charge of 14.0 ± 0.2 mV. The anti-inflammatory and chondr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.158
更新日期：2021-02-15 00:00:00
abstract：:A polyether-urethane based on polytetrahydrofuran containing hydroxypropyl cellulose for biomedical applications was tested for its biocompatibility. Ketoprofen was incorporated (3% and 6%) in the polyurethane matrix as an anti-inflammatory drug. Dynamic vapour sorption method was employed for testing the water sorpti...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.09.026
更新日期：2013-01-01 00:00:00
abstract：:Transdermal drug delivery systems are topically administered medicaments in the form of patches that deliver drugs for systemic effects at a predetermined and controlled rate. It works very simply in which drug is applied inside the patch and it is worn on skin for long period of time. Polymer matrix, drug, permeation...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.05.062
更新日期：2014-08-01 00:00:00
abstract：:In the present study, enhanced pesticidal activity and biocompatibility of chitosan nanocomposite prepared with biocompatible polymer chitosan - insecticidal metabolites derived from potential fungal biopesticidal agent Nomuraea rileyi were studied. Insecticidal metabolites were isolated from the culture filtrate of f...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.130
更新日期：2018-12-01 00:00:00
