Abstract:
:It has been found that the mutator phenotype of the recA441 and recA730 strains that express the SOS response constitutively is suppressed by pIP1, a high-copy plasmid carrying the dnaQ gene encoding the 3'----5' exonuclease subunit (epsilon) of DNA polymerase III. We have constructed plasmid pIP11, in which the dnaQ gene is fused to the strong tac (trp-lac) promoter. Enhanced synthesis of the epsilon subunit stimulated by isopropyl beta-D-thiogalactopyranoside, the inducer of tac, prevents expression of the mutator phenotype of recA441 and markedly decreases the frequency of UV-induced mutations. These results strongly suggest that a loss of editing capacity by the epsilon subunit of DNA polymerase III holoenzyme plays a crucial role in generation of mutations during the SOS response.
journal_name
Proc Natl Acad Sci U S Aauthors
Jonczyk P,Fijalkowska I,Ciesla Zdoi
10.1073/pnas.85.23.9124subject
Has Abstractpub_date
1988-12-01 00:00:00pages
9124-7issue
23eissn
0027-8424issn
1091-6490journal_volume
85pub_type
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