Abstract:
:Sphingolipids (SLs) have been implicated in numerous important cellular biologies; however, their study has been hindered by the complexities of SL metabolism. Furthermore, enzymes of SL metabolism represent a dynamic and interconnected network in which one metabolite can be transformed into other bioactive SLs through further metabolism, resulting in diverse cellular responses. Here we explore the effects of both lethal and sublethal doses of doxorubicin (Dox) in MCF-7 cells. The two concentrations of Dox resulted in the regulation of SLs, including accumulations in sphingosine, sphingosine-1-phosphate, dihydroceramide, and ceramide, as well as reduced levels of hexosylceramide. To further define the effects of Dox on SLs, metabolic flux experiments utilizing a d17 dihydrosphingosine probe were conducted. Results indicated the regulation of ceramidases and sphingomyelin synthase components specifically in response to the cytostatic dose. The results also unexpectedly demonstrated dose-dependent inhibition of dihydroceramide desaturase and glucosylceramide synthase in response to Dox. Taken together, this study uncovers novel targets in the SL network for the action of Dox, and the results reveal the significant complexity of SL response to even a single agent. This approach helps to define the role of specific SL enzymes, their metabolic products, and the resulting biologies in response to chemotherapeutics and other stimuli.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Snider JM,Trayssac M,Clarke CJ,Schwartz N,Snider AJ,Obeid LM,Luberto C,Hannun YAdoi
10.1194/jlr.M089714subject
Has Abstractpub_date
2019-04-01 00:00:00pages
819-831issue
4eissn
0022-2275issn
1539-7262pii
jlr.M089714journal_volume
60pub_type
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
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doi:
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journal_title:Journal of lipid research
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pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
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