Abstract:
:Under specific reaction conditions, levansucrase from Bacillus subtilis (SacB) catalyzes the synthesis of a low molecular weight levan through the non-processive elongation of a great number of intermediates. To deepen understanding of the polymer elongation mechanism, we conducted a meticulous examination of the fructooligosaccharide profile evolution during the levan synthesis. As a result, the formation of primary and secondary intermediates series in different reaction stages was observed. The origin of the series was identified through comparison with product profiles obtained in acceptor reactions employing levanbiose, blastose, 1-kestose, 6-kestose, and neo-kestose, and supported with the isolation and NMR analyses of some relevant products, demonstrating that all of them are inherent products during levan formation from sucrose. These results allowed to establish the network of fructosyl transfer reactions involved in the non-processive levan synthesis. Overall, our results reveal how the relaxed acceptor specificity of SacB during the initial steps of the synthesis is responsible for the formation of several levan series, which constitute the final low molecular weight levan distribution.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Raga-Carbajal E,López-Munguía A,Alvarez L,Olvera Cdoi
10.1038/s41598-018-32872-7subject
Has Abstractpub_date
2018-10-09 00:00:00pages
15035issue
1issn
2045-2322pii
10.1038/s41598-018-32872-7journal_volume
8pub_type
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