Submegabase copy number variations arise during cerebral cortical neurogenesis as revealed by single-cell whole-genome sequencing.

abstract：:Replication of the kinetoplast DNA (kDNA) minicircle of trypanosomatids initiates at a conserved 12-nt sequence, 5'-GGGGTTGGTGTA-3', termed the universal minicircle sequence (UMS). A sequence-specific single-stranded DNA-binding protein from Crithidia fasciculata binds the heavy strand of the 12-mer UMS. Whereas this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Avrahami D,Tzfati Y,Shlomai J

更新日期：1995-11-07 00:00:00

abstract：:Photosystem II (PSII), the light-driven water/plastoquinone photooxidoreductase, is of central importance in the planetary energy cycle. The product of the reaction, plastohydroquinone (PQH2), is released into the membrane from the QB site, where it is formed. A plastoquinone (PQ) from the membrane pool then binds int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Causmaecker S,Douglass JS,Fantuzzi A,Nitschke W,Rutherford AW

更新日期：2019-09-24 00:00:00

abstract：:By analogy with receptor-mediated endocytosis, clathrin-coated membrane segments at locations other than at the plasma membrane have been implicated in intracellular membrane transport/sorting. The crucial role of the Golgi apparatus in these processes prompted us to study the distribution at this level of clathrin-im...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orci L,Ravazzola M,Amherdt M,Louvard D,Perrelet A

更新日期：1985-08-01 00:00:00

abstract：:CCAAT displacement protein (CDP), a nuclear protein of 180-190 kDa, contains a triplicated motif, the cut domain, similar (80-90% conserved) to three repeats of 60-65 amino acids first identified in Drosophila cut, a homeo-domain protein involved in cell-fate decisions in development. Cut repeats bind DNA and exhibit ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aufiero B,Neufeld EJ,Orkin SH

更新日期：1994-08-02 00:00:00

abstract：:Secondary (i.e., heterotrophic or animal) production is a main pathway of energy flow through an ecosystem as it makes energy available to consumers, including humans. Its estimation can play a valuable role in the examination of linkages between ecosystem functions and services. We found that oil and gas platforms of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Claisse JT,Pondella DJ 2nd,Love M,Zahn LA,Williams CM,Williams JP,Bull AS

更新日期：2014-10-28 00:00:00

abstract：:Agrobacterium is a unique model system as well as a major biotechnological tool for genetic manipulation of plant cells. It is still unknown, however, whether host cellular factors exist that are limiting for infection, and whether their overexpression in plant cells can increase the efficiency of the infection. Here,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tzfira T,Vaidya M,Citovsky V

更新日期：2002-08-06 00:00:00

abstract：:Experimental systemic lupus erythematosus (SLE) can be induced in naive mice by immunization with a murine monoclonal anti-DNA antibody (mAb), 5G12, that bears a major idiotype designated 16/6 Id. Strain-dependent differences were observed in the proliferative responses of lymph node cells of mice immunized with two p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waisman A,Ruiz PJ,Israeli E,Eilat E,Könen-Waisman S,Zinger H,Dayan M,Mozes E

更新日期：1997-04-29 00:00:00

abstract：:Specific rabbit antisera against purified bovine pituitary carboxypeptidase processing enzyme (which has also been referred to as "enkephalin convertase") have been prepared and characterized. The antisera recognized both the purified soluble and the membrane-bound forms of the enzyme with equal affinity, suggesting t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hook VY,Mezey E,Fricker LD,Pruss RM,Siegel RE,Brownstein MJ

更新日期：1985-07-01 00:00:00

abstract：:The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ihara M,Furutani S,Shigetou S,Shimada S,Niki K,Komori Y,Kamiya M,Koizumi W,Magara L,Hikida M,Noguchi A,Okuhara D,Yoshinari Y,Kondo S,Tanimoto H,Niwa R,Sattelle DB,Matsuda K

更新日期：2020-07-14 00:00:00

abstract：:The role of the intradiscal polypeptide loops in bovine rhodopsin has been investigated by deletions in the N-terminal tail and in loops B-C, D-E, and E-F as well as by single amino acid substitutions in the D-E loop. Mutants with three types of phenotypes were observed. Type I mutants showed a rhodopsin-like chromoph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doi T,Molday RS,Khorana HG

更新日期：1990-07-01 00:00:00

abstract：:Glucose stimulates insulin secretion from β-cells by increasing intracellular Ca(2+). Ca(2+) then binds to synaptotagmin-7 as a major Ca(2+) sensor for exocytosis, triggering secretory granule fusion and insulin secretion. In type-2 diabetes, insulin secretion is impaired; this impairment is ameliorated by glucagon-li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu B,Wei S,Petersen N,Ali Y,Wang X,Bacaj T,Rorsman P,Hong W,Südhof TC,Han W

更新日期：2015-08-11 00:00:00

abstract：:Treatment of the Daudi Burkitt lymphoma-derived cell line with human interferon alpha, which inhibits cell proliferation in this system, induces differentiation of these B-lymphoid cells into cells with a plasmacytoid phenotype. This differentiation, quantified by the appearance of surface antigens characteristic of m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Exley R,Gordon J,Clemens MJ

更新日期：1987-09-01 00:00:00

abstract：:The anticonvulsants phenytoin and carbamazepine interact allosterically with the batrachotoxin binding site of sodium channels. In the present study, we demonstrate an autoradiographic technique to localize the batrachotoxin binding site on sodium channels in rat brain using [3H]batrachotoxinin-A 20-alpha-benzoate (BT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Worley PF,Baraban JM

更新日期：1987-05-01 00:00:00

abstract：:The nucleotide sequence of the recognition site for the restriction-modification enzyme of Escherichia coli B (SB site) has been determined. The recognition site is a 15-nucleotide sequence consisting of the trimer 5'TGA3', followed by an 8-nucleotide domain of variable sequence, which in turn is followed by tetramer ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ravetch JV,Horiuchi K,Zinder ND

更新日期：1978-05-01 00:00:00

abstract：:The mode of action of "allogeneic supernatant" (the culture supernatant of a 24-hr mixedlymphocyte reaction), has been studied. This factor stimulates the response of spleen cell cultures depleted in thymus-derived lymphoid cells (T-cells) to antigens that elicit a thymus-dependent response. We used a limiting dilutio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hunter P,Kettman JR

更新日期：1974-02-01 00:00:00

abstract：:Mechanism of enzymatic conversion of a fatty acid to the corresponding alkane by the loss of the carboxyl carbon was investigated with particulate preparations from Pisum sativum. A heavy particulate preparation (sp. gr., 1.30 g/cm3) isolated by two density-gradient centrifugation steps catalyzed conversion of octadec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheesbrough TM,Kolattukudy PE

更新日期：1984-11-01 00:00:00

abstract：:It has been proposed that extensive wildfires occurred after the Cretaceous-Tertiary (K-T) impact event. An abundance of soot and pyrosynthetic polycyclic aromatic hydrocarbons (pPAHs) in marine K-T boundary impact rocks (BIRs) have been considered support for this hypothesis. However, nonmarine K-T BIRs, from across ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belcher CM,Finch P,Collinson ME,Scott AC,Grassineau NV

更新日期：2009-03-17 00:00:00

abstract：:The polypeptide chain initiation factor 2 (eIF-2) binds phospholipid (PL) and becomes a potent inhibitor of translation in hemin-supplemented reticulocyte lysates. This binding is markedly reduced by prior treatment of eIF-2 with N-ethylmaleimide. Although PL is probably bound by all three eIF-2 subunits, our results ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Haro C,de Herreros AG,Ochoa S

更新日期：1986-09-01 00:00:00

abstract：:Using an antibody highly specific for D-serine conjugated to glutaraldehyde, we have localized endogenous D-serine in rat brain. Highest levels of D-serine immunoreactivity occur in the gray matter of the cerebral cortex, hippocampus, anterior olfactory nucleus, olfactory tubercle, and amygdala. Localizations of D-ser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schell MJ,Molliver ME,Snyder SH

更新日期：1995-04-25 00:00:00

abstract：:Once-abundant aerial insectivores, such as the Tree Swallow (Tachycineta bicolor), have declined steadily in the past several decades, making it imperative to understand all aspects of their ecology. Aerial insectivores forage on a mixture of aquatic and terrestrial insects that differ in fatty acid composition, speci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Twining CW,Brenna JT,Lawrence P,Shipley JR,Tollefson TN,Winkler DW

更新日期：2016-09-27 00:00:00

abstract：:Protein Hal is a human gamma4 heavy-chain disease protein whose molecular weight is reduced from 55,000 to 25,000 in 6 M guanidine due to the lack of disulfide bonds between heavy chains. Studies of aminoacid sequence indicate that it contains a gap of about 240 residues, starting 10 residues from the N-terminal end a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frangione B,Lee L,Haber E,Bloch KJ

更新日期：1973-04-01 00:00:00

abstract：:Chronic wounds represent a substantial public health problem. The development of tools that would enable sophisticated scrutiny of clinical wound tissue material is highly desirable. This work presents evidence enabling rapid specific identification and laser capture of blood vessels from human tissue in a manner whic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy S,Patel D,Khanna S,Gordillo GM,Biswas S,Friedman A,Sen CK

更新日期：2007-09-04 00:00:00

abstract：:The amino acid sequences of a number of closely related proteins ("napin") isolated from Brassica napus were determined by mass spectrometry without prior separation into individual components. Some of these proteins correspond to those previously deduced (napA, BngNAP1, and gNa), chiefly from DNA sequences. Others we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gehrig PM,Krzyzaniak A,Barciszewski J,Biemann K

更新日期：1996-04-16 00:00:00

abstract：:Motilin, [Met]enkephalin, [Leu]enkephalin, somatostatin, taurine, gamma-aminobutyric acid (GABA), and glycine were tested for their effects on Deiters neurons of the lateral vestibular nucleus in rabbits. Iontophoresis was carried out with multibarrelled micropipettes. All four peptides and three amino acids produced ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan-Palay V,Ito M,Tongroach P,Sakurai M,Palay S

更新日期：1982-05-01 00:00:00

abstract：:Malaria merozoite invasion of human erythrocytes depends on recognition of specific erythrocyte surface receptors by parasite ligands. Plasmodium vivax merozoite invasion is totally dependent on the recognition of the Duffy blood group antigen by the parasite ligand Duffy-binding protein (DBP). Receptor recognition by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： VanBuskirk KM,Sevova E,Adams JH

更新日期：2004-11-02 00:00:00

abstract：:Activation of the CB2 receptor is apparently an endogenous protective mechanism. Thus, it restrains inflammation and protects the skeleton against age-related bone loss. However, the endogenous cannabinoids, as well as Δ(9)-tetrahydrocannabinol, the main plant psychoactive constituent, activate both cannabinoid recept...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smoum R,Baraghithy S,Chourasia M,Breuer A,Mussai N,Attar-Namdar M,Kogan NM,Raphael B,Bolognini D,Cascio MG,Marini P,Pertwee RG,Shurki A,Mechoulam R,Bab I

更新日期：2015-07-14 00:00:00

abstract：:The bacterial pathogen Pseudomonas tolaasii severely damages white button mushrooms by secretion of the pore-forming toxin tolaasin, the main virulence factor of brown blotch disease. Yet, fungus-associated helper bacteria of the genus Mycetocola (Mycetocola tolaasinivorans and Mycetocola lacteus) may protect their ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hermenau R,Kugel S,Komor AJ,Hertweck C

更新日期：2020-09-22 00:00:00

abstract：:There is currently no consensus on the importance of climate change in Mesoamerican prehistory. Some invoke drought as a causal factor in major cultural transitions, including the abandonment of many sites at 900 CE, while others conclude that cultural factors were more important. This lack of agreement reflects the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhattacharya T,Byrne R,Böhnel H,Wogau K,Kienel U,Ingram BL,Zimmerman S

更新日期：2015-02-10 00:00:00

abstract：:The role of geographic isolation in marine microbial speciation is hotly debated because of the high dispersal potential and large population sizes of planktonic microorganisms and the apparent lack of strong dispersal barriers in the open sea. Here, we show that gene flow between distant populations of the globally d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casteleyn G,Leliaert F,Backeljau T,Debeer AE,Kotaki Y,Rhodes L,Lundholm N,Sabbe K,Vyverman W

更新日期：2010-07-20 00:00:00

abstract：:The redox potentials of electron transfer proteins vary over a wide range, even when the type of redox center is the same. Rees [Proc. Natl. Acad. Sci. USA (1985) 82, 3082-3085] proposed that this variation of redox potential partly reflects the different net charges of the proteins, and he presented a linear correlat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore GR,Pettigrew GW,Rogers NK

更新日期：1986-07-01 00:00:00