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Analysis of the yeast SPT3 gene and identification of its product, a positive regulator of Ty transcription.

Abstract：

:Previous work has demonstrated that the yeast SPT3 gene is required for transcription from delta sequences, the long terminal repeats that flank yeast Ty elements. In spt3 null mutants, transcription fails to initiate in delta sequences and instead initiates farther downstream. Null mutations in SPT3 cause other mutant phenotypes, including defects in sporulation and diploid formation. In this paper we report further genetic and physical characterization of the SPT3 gene and protein. By extensive linker insertion mutagenesis, we have delimited the region necessary for SPT3 function. From DNA sequence analysis, SPT3 encodes a protein of 337 amino acids. We have identified this protein with an anti-SPT3 antibody. Finally, we show that overproduction of the SPT3 gene product does not alter the level of Ty transcription.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Winston F,Minehart PL

doi

10.1093/nar/14.17.6885

subject

Has Abstract

pub_date

1986-09-11 00:00:00

pages

6885-900

issue

17

eissn

0305-1048

issn

1362-4962

journal_volume

14

pub_type

杂志文章

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