Abstract：
Background:Schistosoma haematobium which causes urogenital schistosomiasis (UGS) is highly prevalent in African countries. Urine microscopy (UM) is the first-line diagnostic method of UGS. Enzyme-linked immunosorbent assay (ELISA) is a common method for screening many parasite infections primarily or alternatively. The present study established an in-house diagnostic system by ELISA and evaluated its diagnostic efficacy in comparison with UM for screening UGS in White Nile State, Republic of Sudan, 2011-2013. Methods:A total of 490 participants were screened by UM or ELISA, and 149 by both. The in-house ELISA system was established employing soluble egg antigen of S. haematobium and the cut-off absorbance was set at 0.270. Results:Of the 149 subjects, 58 participants (38.9%) were positive by UM, 119 (79.9%) were positive by ELISA and 82 (55.0%) showed consistently positive or negative results by both methods. The diagnostic sensitivity of ELISA was 94.8% and specificity was 29.7% based on UM results. The ELISA positive serum samples also cross-reacted with egg antigens of Schistosoma mansoni and Schistosoma japonicum. Conclusion:We have established in-house ELISA for screening serum immunoglobulin (Ig) G antibodies by employing soluble egg antigen of S. haematobium for diagnosis of UGS with 94.8% sensitivity and 29.7% specificity. The ELISA system can supplement the conventional diagnosis by UM.
journal_name
J Korean Med Scijournal_title
Journal of Korean medical scienceauthors
Song HB,Kim J,Jin Y,Lee JS,Jeoung HG,Lee YH,Saeed AAW,Hong STdoi
10.3346/jkms.2018.33.e238subject
Has Abstractpub_date
2018-08-07 00:00:00pages
e238issue
33eissn
1011-8934issn
1598-6357journal_volume
33pub_type
杂志文章abstract：:A prospective multicenter observational study was performed to investigate the epidemiology and outcomes of community-acquired severe sepsis and septic shock. Subjects included 1,192 adult patients admitted to the 22 participating intensive care units (ICUs) of 12 university hospitals in the Korean Sepsis Registry Sys...
journal_title：Journal of Korean medical science
pub_type： 杂志文章,多中心研究
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abstract：:West Nile encephalitis was first identified in 1937, but until now, it was never diagnosed in Korea. A 58-yr-old Korean man was admitted with headache and cognitive dysfunction. The patient had been on a business trip in Guinea. Cerebrospinal fluid (CSF) showed pleocytosis. The patient complained of both leg weakness,...
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pub_type： 已发布勘误
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journal_title：Journal of Korean medical science
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doi：10.3346/jkms.2003.18.3.349
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doi：10.3346/jkms.2018.33.e1
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journal_title：Journal of Korean medical science
pub_type： 杂志文章
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journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2014.29.8.1166
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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