Intramolecular recombination of linear DNA catalyzed by the Escherichia coli RecE recombination system.

abstract：:DNA methyltransferases flip their target base out of the DNA helix. Here, we have investigated base flipping by wild-type EcoRV DNA methyltransferase (M.EcoRV) and five M.EcoRV variants (D193A, Y196A, S229A, W231R and Y258A). These variants bind to DNA and S-adenosylmethionine but have a severely reduced catalytic eff...

journal_title：Journal of molecular biology

authors： Jeltsch A,Roth M,Friedrich T

更新日期：1999-01-22 00:00:00

abstract：:The NtrC-family PhhR protein of Pseudomonas putida is involved in the control of the metabolism of aromatic amino acids, and it is a dual regulatory protein. When PhhR acts as an activator, it stimulates transcription from its cognate promoters with RNA polymerase/sigma(70) rather than with sigma(54), as is the case f...

journal_title：Journal of molecular biology

authors： Herrera MC,Krell T,Zhang X,Ramos JL

更新日期：2009-12-04 00:00:00

abstract：:Crystal structures of two complexes between recombinant MS2 capsids and RNA operator fragments have been determined at 2.7 A resolution. The coat protein of the RNA bacteriophage MS2 is bifunctional; it forms the icosahedral virus shell to protect the viral nucleic acid and it acts as a translational repressor by bind...

journal_title：Journal of molecular biology

authors： Valegârd K,Murray JB,Stonehouse NJ,van den Worm S,Stockley PG,Liljas L

更新日期：1997-08-01 00:00:00

abstract：:Autophagosomes are double membrane organelles that are formed during a process referred to as macroautophagy. They serve to deliver cytoplasmic material into the lysosome for degradation. Autophagosomes are formed in a de novo manner and are the result of substantial membrane remodeling processes involving numerous pr...

journal_title：Journal of molecular biology

authors： Martens S,Nakamura S,Yoshimori T

更新日期：2016-10-27 00:00:00

abstract：:Our present understanding of the nature of the transition state for protein folding depends predominantly on studies where individual side-chain contributions are mapped out by mutational analysis (phi value analysis). This approach, although extremely powerful, does not in general provide direct information about the...

journal_title：Journal of molecular biology

authors： Sato S,Raleigh DP

更新日期：2007-07-06 00:00:00

abstract：:Eukaryotic replication origins are activated at different times during the S phase of the cell cycle, following a temporal program that is stably transmitted to daughter cells. Although the mechanisms that control initiation at the level of individual origins are now well understood, much less is known on how cells co...

journal_title：Journal of molecular biology

authors： Yoshida K,Poveda A,Pasero P

更新日期：2013-11-29 00:00:00

abstract：:Pathogenic strains of Helicobacter pylori use a type IV secretion system (T4SS) to deliver the toxin CagA into human host cells. The T4SS, along with the toxin itself, is coded into a genomic insert, which is termed the cag pathogenicity island. The cag pathogenicity island contains about 30 open-reading frames, for m...

journal_title：Journal of molecular biology

authors： Cendron L,Couturier M,Angelini A,Barison N,Stein M,Zanotti G

更新日期：2009-02-13 00:00:00

abstract：:Filamentous bacteriophage (Inovirus) is a simple and well-characterized model system. The phage particle, or virion, is about 60 angstroms in diameter and several thousand angstrom units long. The virions are assembled at the bacterial membrane as they extrude out of the host without killing it, an example of specific...

journal_title：Journal of molecular biology

authors： Marvin DA,Welsh LC,Symmons MF,Scott WR,Straus SK

更新日期：2006-01-13 00:00:00

abstract：:To elucidate the interplay between different parts of dimeric single-stranded DNA-binding proteins we have studied the correlated motions in the protein encoded by filamentous phage Pf3 via the combined use of 15N-NMR relaxation experiments, molecular dynamics simulations and essential dynamics calculations. These stu...

journal_title：Journal of molecular biology

authors： Horstink LM,Abseher R,Nilges M,Hilbers CW

更新日期：1999-04-02 00:00:00

abstract：:The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, ...

journal_title：Journal of molecular biology

authors： Stonehouse NJ,Valegård K,Golmohammadi R,van den Worm S,Walton C,Stockley PG,Liljas L

更新日期：1996-02-23 00:00:00

abstract：:The streptococcal promiscuous plasmid pMV158 can be mobilized between a number of bacterial species by means of three elements: (i) the plasmid-encoded nicking-closing protein MobM, involved in the initiation and termination of the conjugative transfer; (ii) the DNA sequence where the MobM-mediated nick takes place (t...

journal_title：Journal of molecular biology

authors： de Antonio C,Farías ME,de Lacoba MG,Espinosa M

更新日期：2004-01-16 00:00:00

abstract：:Quantitative measurements of local chromatin accessibility to DNase I in 15-day chicken embryo erythrocyte nuclei have been performed using a range of nuclease concentrations and real-time TaqMan PCR to monitor the loss of short ( approximately 80 bp) amplicons. At the beta-globin locus, well-established DNase I hyper...

journal_title：Journal of molecular biology

authors： Sapojnikova N,Thorne A,Myers F,Staynov D,Crane-Robinson C

更新日期：2009-02-20 00:00:00

abstract：:Ribosomes are large nucleoproteins of approximately 3 X 10(6) Mr. In contrast to helical or spherical nucleoproteins (viruses) of similar size (which consist of several hundred small asymmetric units reproduced by symmetry), ribosomes are completely asymmetric; therefore, the amount of structural information (defined ...

journal_title：Journal of molecular biology

authors： Hoppe W,Oettl H,Tietz HR

更新日期：1986-11-20 00:00:00

abstract：:A novel interrupted gene motif has been reported in which a 50-nucleotide insertion into bacteriophage T4 gene 60 appears to be present in the message at the time of translation, yet it is not translated. We present here a dynamic model for how translation may be occurring in the neighborhood of the interruption. The ...

journal_title：Journal of molecular biology

authors： Burke-Agüero DH,Hearst JE

更新日期：1990-05-20 00:00:00

abstract：:The structure of a metastable folding intermediate of human insulin-like growth factor I (IGF-I) and an engineered model are investigated by circular dichroism and two-dimensional 1H NMR spectroscopy. The intermediate, which contains two of three native disulfide bonds, was trapped by acid quenching and isolated by re...

journal_title：Journal of molecular biology

authors： Hua QX,Narhi L,Jia W,Arakawa T,Rosenfeld R,Hawkins N,Miller JA,Weiss MA

更新日期：1996-06-07 00:00:00

abstract：:The human immunodeficiency virus type 1 (HIV-1) genome encodes a highly conserved regulatory gene product, Vpr (96 residues, 14kDa), which is incorporated into virions. In the infected cells, Vpr, expressed late in the virus cycle, is believed to function in the early phases of HIV-1 replication, such as nuclear migra...

journal_title：Journal of molecular biology

authors： Morellet N,Bouaziz S,Petitjean P,Roques BP

更新日期：2003-03-14 00:00:00

abstract：:We show that the three core histones H2A, H3 and H4 can transverse lipid bilayers of large unilamellar vesicles (LUVs) and multilamellar vesicles (MLVs). In contrast, the histone H2B, although able to bind to the liposomes, fails to penetrate the unilamellar and the multilamellar vesicles. Translocation across the lip...

journal_title：Journal of molecular biology

authors： Rosenbluh J,Hariton-Gazal E,Dagan A,Rottem S,Graessmann A,Loyter A

更新日期：2005-01-14 00:00:00

abstract：:Titin is a giant, multidomain muscle protein forming a major component of the sarcomere in vertebrate striated muscle. As for many other multidomain proteins, the properties of titin are often studied by characterisation of the constituent domains in isolation. This raises the question of to what extent the properties...

journal_title：Journal of molecular biology

authors： Scott KA,Steward A,Fowler SB,Clarke J

更新日期：2002-01-25 00:00:00

abstract：:A mutant of bovine pancreatic trypsin inhibitor (BPTI) has been constructed and expressed in Escherichia coli in order to probe the kinetic and structural consequences of truncating the binding loop residues to alanine. In addition to two such mutations (Thr11Ala and Pro13Ala), it has a conservative Lys15Arg substitut...

journal_title：Journal of molecular biology

authors： Czapinska H,Otlewski J,Krzywda S,Sheldrick GM,Jaskólski M

更新日期：2000-02-04 00:00:00

abstract：:The yeast retrotransposon Ty1 has structural and functional similarities to retroviruses. We report here that, as in retroviruses, the plus-strand DNA of Ty1 is synthesized as two segments. A central DNA flap is formed during reverse transcription consecutive to elongation (with strand displacement) of the upstream se...

journal_title：Journal of molecular biology

authors： Heyman T,Wilhelm M,Wilhelm FX

更新日期：2003-08-08 00:00:00

abstract：:Activation of family A G-protein-coupled receptors involves a rearrangement of a conserved interhelical cytoplasmic hydrogen bond network between the E(D)RY motif on transmembrane helix 3 (H3) and residues on H6, which is commonly termed the cytoplasmic "ionic lock." Glu134(3.49) of the E(D)RY motif also forms an intr...

journal_title：Journal of molecular biology

authors： Vogel R,Mahalingam M,Lüdeke S,Huber T,Siebert F,Sakmar TP

更新日期：2008-07-18 00:00:00

abstract：:The int gene of phage lambda encodes a protein involved in site-specific recombination. Its expression is regulated differentially during successive phases of the lambda infective cycle. The gene is transcribed early after infection from one promoter, pL, and later from a second promoter pI. Each transcription event r...

journal_title：Journal of molecular biology

authors： Schmeissner U,McKenney K,Rosenberg M,Court D

更新日期：1984-06-15 00:00:00

abstract：:The anticancer drug daunomycin has been co-crystallized with the hexanucleotide duplex sequences d(TGTACA) and d(TGATCA) and single crystal X-ray diffraction studies of these two complexes have been carried out. Structure solution of the d(TGTACA) and d(TGATCA) complexes to 1.6 and 1.7 Angstrom resolution, respectivel...

journal_title：Journal of molecular biology

authors： Nunn CM,Van Meervelt L,Zhang SD,Moore MH,Kennard O

更新日期：1991-11-20 00:00:00

abstract：:Heat capacity curves as obtained from differential scanning calorimetry are an outstanding source for molecular information on protein folding and ligand-binding energetics. However, deconvolution of C(p) data of proteins in the presence of ligands can be compromised by indeterminacies concerning the correct choice of...

journal_title：Journal of molecular biology

authors： Rösgen J,Hinz HJ

更新日期：2001-03-02 00:00:00

abstract：:Scaffold or matrix-attachment regions (S/MARs) are thought to be involved in the organization of eukaryotic chromosomes and in the regulation of several DNA functions. Their characteristics are conserved between plants and humans, and a variety of biological activities have been associated with them. The identificatio...

journal_title：Journal of molecular biology

authors： Bode J,Winkelmann S,Götze S,Spiker S,Tsutsui K,Bi C,A K P,Benham C

更新日期：2006-04-28 00:00:00

abstract：:Three ribosomal RNA mutations conferring resistance to the antibiotic kasugamycin were isolated using a strain of Escherichia coli in which all of the rRNA is transcribed from a plasmid-encoded rrn operon. The mutations, A794G, G926A, and A1519C, mapped to universally conserved sites in the 16 S RNA gene. Site-directe...

journal_title：Journal of molecular biology

authors： Vila-Sanjurjo A,Squires CL,Dahlberg AE

更新日期：1999-10-15 00:00:00

abstract：:We report studies to determine which bacteriophage genes are required for assembly of phage HK97 proheads and what roles they play. We identify the gene encoding the major capsid protein of phage HK97 and report its DNA sequence, together with the DNA sequences of the two genes immediately upstream from it. When the c...

journal_title：Journal of molecular biology

authors： Duda RL,Martincic K,Hendrix RW

更新日期：1995-04-07 00:00:00

abstract：:To understand the phylogenetic relationships between hominoids, the nucleotide sequences of immunoglobulin-epsilon processed pseudogenes from chimpanzee, gorilla and orangutan were determined. The basic structures of these processed pseudogenes agreed with their human counterpart. Although the degrees of nucleotide di...

journal_title：Journal of molecular biology

authors： Ueda S,Watanabe Y,Saitou N,Omoto K,Hayashida H,Miyata T,Hisajima H,Honjo T

更新日期：1989-01-05 00:00:00

abstract：:S100A4 (metastasin) is a member of the S100 family of calcium-binding proteins that is directly involved in tumorigenesis. Until recently, the only structural information available was the solution NMR structure of the inactive calcium-free form of the protein. Here we report the crystal structure of human S100A4 in t...

journal_title：Journal of molecular biology

authors： Pathuri P,Vogeley L,Luecke H

更新日期：2008-10-31 00:00:00

abstract：:The effect of GT/CA dinucleotide repeat tracts on RecA-dependent homologous recombination was examined in vitro. By measuring the binding of RecA protein to oligonucleotides containing GT or CA repeats using the surface plasmon resonance (BIAcore), we show that the efficiency of RecA protein binding is sequence depend...

journal_title：Journal of molecular biology

authors： Dutreix M

更新日期：1997-10-17 00:00:00