EphA2 chimeric antigen receptor-modified T cells for the immunotherapy of esophageal squamous cell carcinoma.

Abstract:

Background:It is urgent to explore an effective potential therapeutic strategy for ESCC. In recent years, cell-based cancer immunotherapy has become a potentially close for carcinoma therapy. Chimeric antigen receptor (CAR) T cell technology is a kind of adoptive cell therapy technique which has been developed rapidly. We sought to obtain EphA2.CAR-T cell and revealed the ability of EphA2.CAR-T cells to kill esophageal squamous cell carcinoma (ESCC) cells in vitro. Methods:Firstly, the expression and location of EphA2 in ESCC tissues and cells was tested by immunohistochemistry staining and Western blot. Secondly, the second generation of EphA2.CAR was constructed via molecular biology technology, and transduced into T cells to obtain the EphA2.CAR-T cell. The transduction efficacies were assessed using flow cytometry (FCM). Thirdly, the effect of cell killing of EphA2.CAR-T cell on ESCC cells in vitro was detected by co-culture experiments. The productions of cytokines (TNF-α and IFN-γ) by EphA2.CAR-T cell after co-culture with ESCC cells were analyzed by ELISA assay. Results:The expression of EphA2 was significantly upregulated in ESCC tissues and cells (P<0.05). EphA2 was expressed on the membrane of ESCC cells, so it could be served as tumor-associated surface antigens (TAA) of CAR for ESCC treatment. The EphA2.CAR-T cell was obtained successfully, and its' transduction efficacies was 61.4% by FCM. The ability of cell killing of EphA2.CAR-T cell was better than that of T cells (P<0.01), and demonstrated a dose-dependent cell killing. The results of ELISA assay showed that the levels of TNF-α and IFN-γ in EphA2.CAR-T cells were notably raised compared with T cells (P<0.05). Conclusions:We firstly constructed the second generation of EphA2.CAR and established EphA2.CAR-T cells. The EphA2.CAR-T cells showed a dose-dependent cell killing of ESCC cells, and promoted the production of cytokines in vitro. These findings open a new way for treatment of ESCC by immunotherapy in the future.

journal_name

J Thorac Dis

authors

Shi H,Yu F,Mao Y,Ju Q,Wu Y,Bai W,Wang P,Xu R,Jiang M,Shi J

doi

10.21037/jtd.2018.04.91

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

2779-2788

issue

5

eissn

2072-1439

issn

2077-6624

pii

jtd-10-05-2779

journal_volume

10

pub_type

杂志文章
  • Transcatheter aortic valve resection: new mechanical devices.

    abstract:Background:To improve periprocedural outcomes of transcatheter aortic valve implantation (TAVI), transcatheter mechanical resection devices were tested for prior ablation of the aortic cusps. Methods:Three mechanical transcatheter resection devices were tested in a series of native porcine (n=30) and reassembled calci...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd-20-2036

    authors: Dai H,Lutter G,Frank D,Freitag-Wolf S,Topal A,Haneya A,Sathananthan J,Puehler T

    更新日期:2020-11-01 00:00:00

  • Options for temporary mechanical circulatory support.

    abstract::Temporary mechanical circulatory support (MCS) refers to a group of devices generally used for less than 30 days to maintain adequate organ perfusion by compensating for a failure of the pumping mechanism of the heart. The increased availability and rapid adoption of new temporary MCS strategies necessitate physicians...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.3978/j.issn.2072-1439.2015.09.14

    authors: Saffarzadeh A,Bonde P

    更新日期:2015-12-01 00:00:00

  • Transcatheter aortic valve implantation for patients with lung cancer and aortic valve stenosis.

    abstract::Many patients who require lung resection have cardiovascular and cerebrovascular comorbidities. It has been recommended that surgical aortic valve replacement (SAVR) should precede lung resection in patients with severe aortic valve stenosis (AS). However, by first undergoing transcatheter aortic valve implantation (T...

    journal_title:Journal of thoracic disease

    pub_type:

    doi:10.21037/jtd.2018.04.83

    authors: Sakai T,Yahagi K,Miura S,Hoshino T,Yokota T,Tanabe K,Ikeda S

    更新日期:2018-05-01 00:00:00

  • Robot-assisted thymectomy via subxiphoid approach: technical details and early outcomes.

    abstract:Background:Trans-subxiphoid thoracoscopic thymectomy is a promising procedure but technically demanding associated with ergonomic discomfort. In order to facilitate this complex procedure, the authors present a modified trans-subxiphoid thymectomy by the use of the Da Vinci robotic system. Methods:A 2-cm longitudinal ...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2018.03.07

    authors: Zhang H,Chen L,Zheng Y,Wang Z,Geng Y,Wang F,Liu D,He A,Li J,Wang Y

    更新日期:2018-03-01 00:00:00

  • Effectiveness and safety of diagnostic flexi-rigid thoracoscopy in differentiating exudative pleural effusion of unknown etiology: a retrospective study of 215 patients.

    abstract:BACKGROUND:The aim of this study was to evaluate the effectiveness and safety of diagnostic flexi-rigid thoracoscopy in differentiating exudative pleural effusion of unknown etiology. METHODS:A total of 215 patients with undiagnosed exudative pleural effusion were consecutively recruited between January 2011 and Febru...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.3978/j.issn.2072-1439.2014.02.09

    authors: Gao BA,Zhou G,Guan L,Zhang LY,Xiang GM

    更新日期:2014-05-01 00:00:00

  • Gabapentin versus baclofen for treatment of refractory gastroesophageal reflux-induced chronic cough.

    abstract::Refractory gastroesophageal reflux-induced chronic cough (GERC) is a special type of gastroesophageal reflux disease (GERD) with predominant cough resistant to pragmatic standard anti-reflux therapy including antisecretory agents alone or in combination with promotility agents but with a favorable response to intensif...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.21037/jtd-2020-icc-002

    authors: Zhang M,Zhu Y,Dong R,Qiu Z

    更新日期:2020-09-01 00:00:00

  • Risk factors for obstructive sleep apnea-related hypertension in police officers in Southern China.

    abstract:Background:Obstructive sleep apnea (OSA) is an independent risk factor for hypertension. OSA-related hypertension is common and treatment of OSA can reduce blood pressure (BP). However, few studies have been conducted on this type of hypertension in the Chinese occupational population. Methods:We conducted a field inv...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2019.09.83

    authors: Pan M,Ou Q,Chen B,Hong Z,Liu H

    更新日期:2019-10-01 00:00:00

  • Mid-term results of completely portal robotic lobectomy for stage I & II non-small cell lung cancer.

    abstract:Background:Minimally invasive robotic-assisted thoracic surgery is an increasingly popular platform for oncological thoracic resection. The aim of this study is to evaluate the feasibility of completely portal robotic lobectomy for patients with early non-small cell lung cancer (NSCLC), analysing the perioperative and ...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd-20-1915

    authors: Ong K,Fazuludeen AA,Ahmed ADB

    更新日期:2020-10-01 00:00:00

  • Thyroid cancer diagnosis in the era of precision imaging.

    abstract::Thyroid cancer affects 1.3% of the population with increasing rates of incidence over the last decade (approximately 2% per year). Although the overall prognosis is good in the differentiated subtypes, there has been a slow but steady increase in rate of deaths associated with thyroid cancer (approximately 0.7% per ye...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.21037/jtd.2019.08.37

    authors: Bonjoc KJ,Young H,Warner S,Gernon T,Maghami E,Chaudhry A

    更新日期:2020-09-01 00:00:00

  • Efficacy of epidermal growth factor receptor-tyrosine kinase inhibitors for Chinese patients with squamous cell carcinoma of lung harboring EGFR mutation.

    abstract:OBJECTIVE:Epidermal growth factor receptor (EGFR) mutation mostly occurred in lung adenocarcinoma, rarely in squamous cell carcinoma (SQCC). EGFR mutation rate in SQCC varied in previous reports, and the efficacy of EGFR tyrosine kinase inhibitors (TKIs) in SQCC harboring EGFR mutation has not yet been fully evaluated....

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.3978/j.issn.2072-1439.2013.09.15

    authors: Fang W,Zhang J,Liang W,Huang Y,Yan Y,Wu X,Hu Z,Ma Y,Zhao H,Zhao Y,Yang Y,Xue C,Zhang J,Zhang L

    更新日期:2013-10-01 00:00:00

  • Association between blood eosinophils with exacerbation and patient-reported outcomes in chronic obstructive pulmonary disease patients in an endemic area for parasitic infections: a prospective study.

    abstract:Background:Eosinophilic chronic obstructive pulmonary disease (COPD) patients have eosinophilic airway inflammation. No prospective study has reported blood eosinophil counts in an endemic area for parasitic infection. The primary objective was to compare exacerbation rates. The secondary objectives were patient-report...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd-19-4101

    authors: Juthong S,Kaenmuang P

    更新日期:2020-09-01 00:00:00

  • Perioperative change in neutrophil-to-lymphocyte ratio (NLR) is a prognostic factor in patients with completely resected primary pulmonary sarcomatoid carcinoma.

    abstract:Background:There has been controversy regarding prognostic factors for surgically resected primary pulmonary sarcomatoid carcinoma (PSC). Recently, several studies have shown that neutrophil-to-lymphocyte ratio (NLR) was a prognostic factor for various types of cancers from multiple organs. Therefore, we performed this...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2019.02.02

    authors: Seong YW,Han SJ,Jung W,Jeon JH,Cho S,Jheon S,Kim K

    更新日期:2019-03-01 00:00:00

  • Benign esophago-pulmonary fistula complicating achalasia: case report and literature review.

    abstract::The achalasia, a common benign disease of esophagus, plays an important role in esophago-respiratory fistula, which has not yet been well recognized. In present study, a case is reported of a patient with benign esophago-pulmonary fistula secondary to a longstanding achalasia. To our knowledge, this is the fifth patie...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.3978/j.issn.2072-1439.2015.04.03

    authors: Zhu J,Ni Y,Lu Q,Li X,Wang W,Wang H

    更新日期:2015-04-01 00:00:00

  • Treatment choice by patients with obstructive sleep apnea: data from two centers in China.

    abstract:Background:Standard management has been recommended for obstructive sleep apnea (OSA) by several guidelines, but patient choice in the practical setting is unclear. Methods:A survey nested in two prospective cohort studies of OSA (enrollment: 2001-2010) in China. The last interview was conducted between July 2014 and ...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2018.03.135

    authors: Liao WJ,Song LJ,Yi HL,Guan J,Zou JY,Xu HJ,Wang G,Ma F,Zhou LB,Chen YQ,Yan LB,Deng ZC,McNicholas WT,Yin SK,Zhong NS,Zhang XW

    更新日期:2018-03-01 00:00:00

  • Mitral valve prolapse: an underestimated cause of sudden cardiac death-a current review of the literature.

    abstract::Mitral valve prolapse (MVP) is a common valve abnormality in general population. Despite the general belief of a benign disorder, several articles since the 1980s report sudden cardiac death (SCD) in MVP patients, with a substantial percentage of asymptomatic young individuals. The problem is to detect those patients ...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.21037/jtd.2017.11.14

    authors: Spartalis M,Tzatzaki E,Spartalis E,Athanasiou A,Moris D,Damaskos C,Garmpis N,Voudris V

    更新日期:2017-12-01 00:00:00

  • Air pollution, weather variations and primary spontaneous pneumothorax.

    abstract:BACKGROUND:Spontaneous pneumothoraces (SP) tend to occur in clusters which have been related to atmospheric pressure variations and thunderstorm insurgence. We examined the influence of standard meteorological parameter variations and concentrations of the major air pollutants on incidence of spontaneous pneumothorax (...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:

    authors: Bertolaccini L,Alemanno L,Rocco G,Cassardo C

    更新日期:2010-03-01 00:00:00

  • An approach to antibiotic treatment in patients with sepsis.

    abstract::Sepsis is a medical emergency and life-threatening condition due to a dysregulated host response to infection, which is time-dependent and associated with unacceptably high mortality. Thus, when treating suspicious or confirmed cases of sepsis, clinicians must initiate broad-spectrum antimicrobials within the first ho...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.21037/jtd.2020.01.47

    authors: Martínez ML,Plata-Menchaca EP,Ruiz-Rodríguez JC,Ferrer R

    更新日期:2020-03-01 00:00:00

  • Anomalous right coronary artery origin with interarterial pathway-importance of morphological origin assessment and the role of percutaneous interventionism.

    abstract::Anomalies in coronary arteries (CA) are an important issue in cardiology and cardiovascular surgery. Specifically the anomalous origin of the right coronary artery (RCA) is of special importance because it is the most frequent anomaly. Clinical practice guidelines recommend the revascularization treatment in cases of ...

    journal_title:Journal of thoracic disease

    pub_type:

    doi:10.21037/jtd.2017.05.15

    authors: García Iglesias D,Martínez Fernández L,Martín Fernández M,García Suárez L,García Pérez L,Calvo Blanco J,Cigarrán Sexto H,Del Valle Fernández R,Morís de la Tassa C

    更新日期:2017-05-01 00:00:00

  • Bronchial aneurysm secondary to tuberculosis presenting with fatal hemoptysis: a case report and review of the literature.

    abstract::Fatal hemoptysis is a serious complication of pulmonary tuberculosis, which can rarely be due to a bronchial aneurysm. Here, we present a case of fatal hemoptysis due to bronchial aneurysm occurred in a 59-year-old patient of Han ethnicity suffering from active pulmonary tuberculosis. Meanwhile, we perform a literatur...

    journal_title:Journal of thoracic disease

    pub_type:

    doi:10.3978/j.issn.2072-1439.2014.04.07

    authors: Cheng YS,Lu ZW

    更新日期:2014-06-01 00:00:00

  • Bridging to lung transplantation with extracorporeal circulatory support: when or when not?

    abstract::Patients with end-stage lung disease who are candidates for lung transplantation may acutely decompensate before a donor organ becomes available. In this scenario, extracorporeal life support (ECLS) may be considered as a bridge to transplant or as a bridge to decision. In the current chapter, we review the indication...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.21037/jtd.2017.08.117

    authors: Loor G,Simpson L,Parulekar A

    更新日期:2017-09-01 00:00:00

  • Diagnostic value of CD206+CD14+ macrophages in diagnosis of lung cancer originated malignant pleural effusion.

    abstract:Background:Pleural effusions are common complications of various diseases. Patients with malignant pleural effusion (MPE) usually face poor prognosis and short life expectancy. Discriminating between MPE and benign pleural effusion remains to be difficult. The aim of our current study was to evaluate whether CD206+CD14...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2019.06.44

    authors: Pei XB,Wu XZ,Yi FS,Zhai K,Shi HZ

    更新日期:2019-07-01 00:00:00

  • Vitamin C alone does not improve treatment outcomes in mechanically ventilated patients with severe sepsis or septic shock: a retrospective cohort study.

    abstract:Background:Vitamin C has shown several beneficial effects on sepsis in preclinical studies. However, clinical data supporting these reports are scarce. This study aimed to evaluate whether adjunctive intravenous vitamin C therapy could reduce hospital mortality in patients with severe sepsis or septic shock requiring m...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2019.03.03

    authors: Ahn JH,Oh DK,Huh JW,Lim CM,Koh Y,Hong SB

    更新日期:2019-04-01 00:00:00

  • Human embryonic lung fibroblasts treated with artesunate exhibit reduced rates of proliferation and human cytomegalovirus infection in vitro.

    abstract:BACKGROUND:Cytomegalovirus (CMV) pneumonia is a major cause of death in immunosuppressed patients. Despite the effective treatment with ganciclovir (GCV) and other antiviral agents, the mortality rate remains between 30% to 50%. Recently, the anti-malarial drug artesunate (ART) wasfound to exhibit significant anti-vira...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.3978/j.issn.2072-1439.2015.07.05

    authors: Zeng AH,Ou YY,Guo MM,Dai X,Zhou DZ,Chen R

    更新日期:2015-07-01 00:00:00

  • One-stage hybrid surgery for acute Stanford type A aortic dissection with David operation, aortic arch debranching, and endovascular graft: a case report.

    abstract::Acute Stanford type A aortic dissection requires an extremely complex surgical strategy and presents high risk of complications. Although many different procedures were reported to treat this aortic dissection, high mortality rate and incidences of complications still exist. This study presents a 59-year-old lady with...

    journal_title:Journal of thoracic disease

    pub_type:

    doi:10.21037/jtd.2016.12.81

    authors: Liu L,Qin C,Hou J,Zhu D,Zhang B,Ma H,Guo Y

    更新日期:2016-12-01 00:00:00

  • In vitro comparison of everting vs. non-everting suture techniques for the implantation of a supra-annular biological heart valve.

    abstract:Background:The aim of this study was to evaluate the hemodynamic effect of different suturing techniques for aortic valve replacement (AVR) in vitro. Whether or not the applied suturing technique impacts the outflow tract diameter by narrowing the annulus diameter was examined. Methods:The commonly applied non-evertin...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2020.03.55

    authors: Puluca N,Münsterer A,Prinzing A,Sexton ZA,Lange R,Meyer-Saraei R,Scharfschwerdt M

    更新日期:2020-05-01 00:00:00

  • The nationwide program of allergic disease prevention as an implementation of GARD guidelines in Poland.

    abstract:Background:In 2006, the Global Alliance against Chronic Respiratory Diseases (GARD) was established. The GARD strategy is based mainly on activities aimed at implementing preventive tasks at a local and global level. In Poland, the National Health Program (NHP) is the strategic document describing the tasks of governme...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd.2018.08.97

    authors: Raciborski F,Samoliński B,Krzych-Fałta E,Grąbczewska A,Furman F,Bieszczad M,Morkisz P,Witkowska P,Bousquet J,Gujski M

    更新日期:2018-09-01 00:00:00

  • Hybrid esophagectomy.

    abstract::Esophagectomy is a complex surgical procedure associated with high rates of mortality and morbidity, mainly dominated by pulmonary complications. Minimally invasive approaches have been developed in order to decrease postoperative morbidity, including totally minimally invasive esophagectomy (MIE) and hybrid esophagec...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.21037/jtd.2018.12.92

    authors: Voron T,Lintis A,Piessen G

    更新日期:2019-04-01 00:00:00

  • Type 2 diabetes is associated with failure of non-operative treatment for sternoclavicular joint infection.

    abstract:Background:A standardized treatment algorithm for sternoclavicular joint infection management is lacking in the literature. While major risk factors for sternoclavicular joint infection, including immunosuppression, rheumatoid arthritis, type 2 diabetes, indwelling catheters, and intravenous drug use have been identifi...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.21037/jtd-20-1897

    authors: Reddy SB,Mizelle J,Sterbling HM,Lin B,Litle VR,Suzuki K

    更新日期:2020-10-01 00:00:00

  • Effects of bronchoscopic lung volume reduction using transbronchial infusion of autologous blood and thrombin in patients with severe chronic obstructive pulmonary disease.

    abstract:BACKGROUND:Existing medical treatments have limitations in the management of very severe chronic obstructive pulmonary disease (COPD). METHODS:We performed bronchoscopic lung volume reduction (BLVR) using transbronchial infusion of autologous blood and thrombin (BLVR with blood) in three patients with very severe COPD...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章

    doi:10.3978/j.issn.2072-1439.2015.01.10

    authors: Mizumori Y,Mochiduki Y,Nakahara Y,Kawamura T,Sasaki S,Morimoto A,Tsukamoto H

    更新日期:2015-03-01 00:00:00

  • Surgery and pleuro-pulmonary tuberculosis: a scientific literature review.

    abstract::Tuberculosis (TB) is still a major public health concern, mostly affecting resource-constrained settings and marginalized populations. The fight against the disease is hindered by the growing emergence of drug-resistant forms whose management can be rather challenging. Surgery may play an important role to support dia...

    journal_title:Journal of thoracic disease

    pub_type: 杂志文章,评审

    doi:10.21037/jtd.2016.05.59

    authors: Subotic D,Yablonskiy P,Sulis G,Cordos I,Petrov D,Centis R,D'Ambrosio L,Sotgiu G,Migliori GB

    更新日期:2016-07-01 00:00:00