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In vitro proinflammatory gene expression predicts in vivo telomere shortening: A preliminary study.

Abstract：

:The chronic psychological stress of caregiving leads to higher risks for many diseases. One of the mechanisms through which caregiving is associated with disease risk is chronic inflammation. Chronic inflammation may accelerate cellular aging via telomere dysfunction and cell senescence, although this has not been examined in human cells from healthy people. We examined peripheral blood mononuclear cells (PBMCs) from 20 healthy mothers of children with autism (caregivers) and 19 mothers of neurotypical children (controls) in an in vitro culture system where PBMCs were stimulated with phytohaemagglutinin (PHA). We measured RNA expression levels of a panel of immune function genes before and after PHA stimulation, as well as telomere length from PBMCs collected from the participants at baseline and 15 months later. Caregivers and controls had similar gene expression profiles in unstimulated PBMCs, but after PHA stimulation, caregivers had increased RNA levels of the master inflammatory regulator NF-κB and its proinflammatory cytokine targets IL-1β, IL-6 and its receptor IL-6R as well as inflammatory chemokines IL-8, CXCL1 and CXCL2. Gene expression analysis suggested caregivers have increased Treg and Th17 T cell differentiation. Additionally, key signaling molecules involved in the upregulation of COX-2, a critical enzyme in the synthesis of the inflammatory mediator prostaglandin, were elevated. When both groups were examined together, higher expression levels of proinflammatory genes were associated with shorter telomere length in PBMCs from blood drawn 15 months later, independent of baseline telomere length. Taken together, these results suggest that chronic stress is associated with an exaggerated inflammatory response in PBMCs, which in turn is associated with shorter telomere length measured from PBMCs collected 15 months later. To our knowledge, this is the first human study that shows increased proinflammatory expression predicts future telomere shortening.

journal_name

Psychoneuroendocrinology

journal_title

Psychoneuroendocrinology

authors

Lin J,Sun J,Wang S,Milush JM,Baker CAR,Coccia M,Effros RB,Puterman E,Blackburn E,Prather AA,Epel E

doi

10.1016/j.psyneuen.2018.06.020

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

179-187

eissn

0306-4530

issn

1873-3360

pii

S0306-4530(18)30115-X

journal_volume

pub_type

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In vitro proinflammatory gene expression predicts in vivo telomere shortening: A preliminary study.