Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

abstract：:Species distribution models (SDMs) have become an essential tool in ecology, biogeography, evolution and, more recently, in conservation biology. How to generalize species distributions in large undersampled areas, especially with few samples, is a fundamental issue of SDMs. In order to explore this issue, we used the...

journal_title：PeerJ

authors： Mi C,Huettmann F,Guo Y,Han X,Wen L

更新日期：2017-01-12 00:00:00

abstract：Background:Patellofemoral pain (PFP) is often reported as a diffuse pain at the front of the knee during knee-loading activities. A patient's description of pain location and distribution is commonly drawn on paper by clinicians, which is difficult to quantify, report and compare within and between patients. One way of...

journal_title：PeerJ

authors： Matthews M,Rathleff MS,Vicenzino B,Boudreau SA

更新日期：2018-03-08 00:00:00

abstract：Background:Forest ecosystems are considered among the largest terrestrial carbon sinks. The dynamics of forest carbon depend on where the carbon is stored and its responses to environmental factors, as well as the physiology of the trees. Thus, threatened forest regions with high biodiversity have great scientific impo...

journal_title：PeerJ

authors： González-Cásares M,Pompa-García M,Venegas-González A,Domínguez-Calleros P,Hernández-Díaz J,Carrillo-Parra A,González-Tagle M

更新日期：2019-06-11 00:00:00

abstract：Background:The need for read-based phasing arises with advances in sequencing technologies. The minimum error correction (MEC) approach is the primary trend to resolve haplotypes by reducing conflicts in a single nucleotide polymorphism-fragment matrix. However, it is frequently observed that the solution with the opti...

journal_title：PeerJ

authors： Lin YY,Wu PC,Chen PL,Oyang YJ,Chen CY

更新日期：2018-10-30 00:00:00

abstract：Background:Oil spills can cause severe damage within a marine ecosystem. Following a spill, the soluble fraction of polycyclic aromatic hydrocarbons is rapidly released into the water column. These remain dissolved in seawater over an extended period of time, even should the insoluble fraction be removed. The vertical ...

journal_title：PeerJ

authors： Kryzevicius Z,Mickuviene K,Bucas M,Vilkiene M,Zukauskaite A

更新日期：2020-10-27 00:00:00

abstract：Background:Anaplastic thyroid carcinoma (ATC) is a rare malignant tumor with a poor prognosis. However, there is no useful clinical prognostic predictive tool for ATC so far. Our study identified risk factors for survival of ATC and created a reliable nomogram to predict overall survival (OS) and cancer-specific surviv...

journal_title：PeerJ

authors： Gui W,Zhu W,Lu W,Shang C,Zheng F,Lin X,Li H

更新日期：2020-05-21 00:00:00

abstract：:One of the best ex situ conservation strategies for wild germplasm is in vitro conservation of genetic banks. The success of in vitro conservation relies heavily on the micropropagation or performance of the species of interest. In the context of global change, crop production challenges and climate change, we face a ...

journal_title：PeerJ

authors： Hernández-Terán A,Wegier A,Benítez M,Lira R,Sosa Fuentes TG,Escalante AE

更新日期：2019-06-10 00:00:00

abstract：Background:Carbon (C), nitrogen (N) and phosphorus (P) stoichiometric ratios are important indicators of ecosystem function and productivity. However, few studies have assessed the nutrient relationship between plant, litter and soil, and the nutrient stock in different secondary mixed forest types. Methods:We investi...

journal_title：PeerJ

authors： Pang Y,Tian J,Zhao X,Chao Z,Wang Y,Zhang X,Wang D

更新日期：2020-06-03 00:00:00

abstract：:The purpose of the study was to investigate the relationship between different exercise modes and visuospatial working memory in healthy older adults. A cross-sectional design was adopted. A total of 111 healthy older adults were enrolled in the study. They were classified by the exercise-related questionnaire to be i...

journal_title：PeerJ

authors： Guo W,Wang B,Lu Y,Zhu Q,Shi Z,Ren J

更新日期：2016-07-20 00:00:00

abstract：Background:Proper glycemic control is an important goal of critical care medicine, including perioperative patient care that can influence patients' prognosis. Insulin secretion from pancreatic β-cells is generally assumed to play a critical role in glycemic control in response to an elevated blood glucose concentratio...

journal_title：PeerJ

authors： Kusunoki M,Hayashi M,Shoji T,Uba T,Tanaka H,Sumi C,Matsuo Y,Hirota K

更新日期：2019-12-02 00:00:00

abstract：BACKGROUND:The human microbiota plays a key role in health and disease, and bacteriocins, which are small, bacterially produced, antimicrobial peptides, are likely to have an important function in the stability and dynamics of this community. Here we examined the density and distribution of the subclass I lantibiotic m...

journal_title：PeerJ

authors： Walsh CJ,Guinane CM,O' Toole PW,Cotter PD

更新日期：2017-04-27 00:00:00

abstract：:Pico and nanoplankton communities from the Southwest Atlantic Ocean along the Brazilian Bight are poorly described. The hydrography in this region is dominated by a complex system of layered water masses, which includes the warm and oligotrophic Tropical Water (TW), the cold and nutrient rich South Atlantic Central Wa...

journal_title：PeerJ

authors： Gérikas Ribeiro C,Lopes Dos Santos A,Marie D,Helena Pellizari V,Pereira Brandini F,Vaulot D

更新日期：2016-11-10 00:00:00

abstract：:The etiology of cancer includes aberrant cellular homeostasis where a compromised RNA regulatory network is a prominent contributing factor. In particular, noncoding RNAs including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) were recently shown to play important roles in the initiation, progression, and metas...

journal_title：PeerJ

authors： Ning P,Wu Z,Hu A,Li X,He J,Gong X,Xia Y,Shang Y,Bian H

更新日期：2018-01-12 00:00:00

abstract：Background:Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intrac...

journal_title：PeerJ

authors： Bhavsar MB,Cato G,Hauschild A,Leppik L,Costa Oliveira KM,Eischen-Loges MJ,Barker JH

更新日期：2019-02-08 00:00:00

abstract：Background and Purpose:Oxidative stress is involved in normal and pathological functioning of skeletal muscle. Protection of myoblasts from oxidative stress may improve muscle contraction and delay aging. Here we studied the effect of R. coriaria sumac fruit extract on human myoblasts and zebrafish embryos in condition...

journal_title：PeerJ

authors： Najjar F,Rizk F,Carnac G,Nassar R,Jabak S,Sobolev AP,Bou Saada Y,El Sabban M,Hamade A

更新日期：2017-12-12 00:00:00

abstract：:Nitrogen-fixing microorganisms play important roles in N cycling. However, knowledge related to the changes in the diazotrophic community in response to cropping systems is still rudimentary. In this study, the nifH gene was used to reveal the abundance and community compositions of diazotrophs in the cropping systems...

journal_title：PeerJ

authors： Zou J,Yao Q,Liu J,Li Y,Song F,Liu X,Wang G

更新日期：2020-07-15 00:00:00

abstract：:While there have been increasing numbers of reports of human-wolf conflict in China during recent years, little is known about the nature of this conflict. In this study, we used questionnaires and semi-structured interviews to investigate local villagers' perceptions of wolves in Jiuzhaigou County, western China. We ...

journal_title：PeerJ

authors： Xu Y,Yang B,Dou L

更新日期：2015-06-09 00:00:00

abstract：:Pueraria lobata (willd.) Ohwi is a consumable selenium-enriched plant used for medicinal purposes. The molecular response to selenium (Se) stimuli in P. lobata is currently unknown. We used RNA-Seq to identify potential genes involved in selenite metabolism and analyzed their expression profiles. We obtained a total o...

journal_title：PeerJ

authors： Guo K,Yao Y,Yang M,Li Y,Wu B,Lin X

更新日期：2020-03-24 00:00:00

abstract：:Sex reversal is a complex biological phenomenon exhibited by Cynoglossus semilaevis. Some genetic females may irreversibly convert to pseudomales, thus increasing aquaculture costs because males grow much more slowly than females. In this study, an integrative analysis of transcriptome and proteome was performed to co...

journal_title：PeerJ

authors： Ye Z,Wang W,Zhang Y,Wang L,Cui Y,Li H

更新日期：2020-03-19 00:00:00

abstract：:The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most serious citrus pests worldwide due to its role as vector of huanglongbing or citrus greening disease. While some optimal plant species for ACP oviposition and development have been identified, little is known of the influence of host plants ...

journal_title：PeerJ

authors： Paris TM,Allan SA,Hall DG,Hentz MG,Hetesy G,Stansly PA

更新日期：2016-11-03 00:00:00

abstract：:Osteogenesis imperfecta (OI) is a genetic disorder that is usually caused by disturbed production of collagen type I. Depending on its severity in the patient, this disorder may create difficulties and challenges for the dental practitioner. The goal of this article is to provide guidelines based on scientific evidenc...

journal_title：PeerJ

authors： Rousseau M,Retrouvey JM,Members of the Brittle Bone Disease Consortium.

更新日期：2018-08-17 00:00:00

abstract：:As other spiny lobsters, Panulirus argus is supposed to use preferentially proteins and lipids in energy metabolism, while carbohydrates are well digested but poorly utilized. The aim of this study was to evaluate the effect of dietary carbohydrate level on digestion and metabolism in the spiny lobster P. argus. We us...

journal_title：PeerJ

authors： Rodríguez-Viera L,Perera E,Montero-Alejo V,Perdomo-Morales R,García-Galano T,Martínez-Rodríguez G,Mancera JM

更新日期：2017-11-03 00:00:00

abstract：Background:Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Methods:Forty-two patients with periodontitis Stage IV Grade C ...

journal_title：PeerJ

authors： Liu K,Han B,Hou J,Zhang J,Su J,Meng H

更新日期：2021-01-04 00:00:00

abstract：Background:Ontario, Canada is home to eight native species of turtles; all eight are federally listed as Species At Risk, due to anthropogenic threats. However, until recently, reports of infectious disease have been lacking. Ranavirus is seen as an emerging threat for ectotherms globally, with mass die-offs most often...

journal_title：PeerJ

authors： Carstairs SJ

更新日期：2019-05-20 00:00:00

abstract：:Over the last decades several studies have identified that the directional changes in climate induced by anthropogenic emissions of greenhouse gases are affecting the ecology of desert ecosystems. In the Southwest United States, the impacts of climate change to plant abundance and distribution have already been report...

journal_title：PeerJ

authors： Albuquerque F,Benito B,Rodriguez MÁM,Gray C

更新日期：2018-09-19 00:00:00

abstract：:In this study, four experimental assays were conducted to evaluate the use of a new silver nanoparticle formulation named Argovit-4, which was prepared with slight modifications to enhance its biological activity against white spot syndrome virus (WSSV) in shrimp culture. The goals of these assays were to (1) determin...

journal_title：PeerJ

authors： Romo-Quiñonez CR,Álvarez-Sánchez AR,Álvarez-Ruiz P,Chávez-Sánchez MC,Bogdanchikova N,Pestryakov A,Mejia-Ruiz CH

更新日期：2020-02-27 00:00:00

abstract：:The selection and validation of proper distinguishing characters are of crucial importance in taxonomic revisions. The modern classifications of orchids utilize the molecular tools, but still the selection and identification of the material used in these studies is for the most part related to general species morpholo...

journal_title：PeerJ

authors： Jakubska-Busse A,Żołubak E,Łobas Z,Gola EM

更新日期：2017-07-21 00:00:00

abstract：Background:WRKY proteins play a vital role in the plants response to different stresses, growth and development. Studies of WRKY proteins have been mainly focused on model plant Arabidopsis and a few other vegetable plants. However, the systematical study of eggplant WRKY transcription factor superfamily is scarce. Me...

journal_title：PeerJ

authors： Yang Y,Liu J,Zhou X,Liu S,Zhuang Y

更新日期：2020-03-17 00:00:00

abstract：:Stridulatory signals are involved in conspecific interactions between bark beetles (Coleoptera: Curculionidae, Scolytinae). In this study, we compared the qualitative profiles of acoustic signals in three species from the genus Polygraphus Er. Sympatry can be periodically observed in two of them -P. proximus and P. su...

journal_title：PeerJ

authors： Kerchev IA

更新日期：2020-01-02 00:00:00

abstract：Aim:Historically, the distribution of Sandhill Cranes included much of North America and extending in summer into northeast Russia. In recent years, observations of sandhill cranes in Asia during the non-breeding period have been frequently reported. However, the distribution and abundance of sandhill cranes during the...

journal_title：PeerJ

authors： Gao L,Mi C,Guo Y

更新日期：2019-08-30 00:00:00