A Profile Hidden Markov Model to investigate the distribution and frequency of LanB-encoding lantibiotic modification genes in the human oral and gut microbiome.

Abstract:

BACKGROUND:The human microbiota plays a key role in health and disease, and bacteriocins, which are small, bacterially produced, antimicrobial peptides, are likely to have an important function in the stability and dynamics of this community. Here we examined the density and distribution of the subclass I lantibiotic modification protein, LanB, in human oral and stool microbiome datasets using a specially constructed profile Hidden Markov Model (HMM). METHODS:The model was validated by correctly identifying known lanB genes in the genomes of known bacteriocin producers more effectively than other methods, while being sensitive enough to differentiate between different subclasses of lantibiotic modification proteins. This approach was compared with two existing methods to screen both genomic and metagenomic datasets obtained from the Human Microbiome Project (HMP). RESULTS:Of the methods evaluated, the new profile HMM identified the greatest number of putative LanB proteins in the stool and oral metagenome data while BlastP identified the fewest. In addition, the model identified more LanB proteins than a pre-existing Pfam lanthionine dehydratase model. Searching the gastrointestinal tract subset of the HMP reference genome database with the new HMM identified seven putative subclass I lantibiotic producers, including two members of the Coprobacillus genus. CONCLUSIONS:These findings establish custom profile HMMs as a potentially powerful tool in the search for novel bioactive producers with the power to benefit human health, and reinforce the repertoire of apparent bacteriocin-encoding gene clusters that may have been overlooked by culture-dependent mining efforts to date.

journal_name

PeerJ

journal_title

PeerJ

authors

Walsh CJ,Guinane CM,O' Toole PW,Cotter PD

doi

10.7717/peerj.3254

subject

Has Abstract

pub_date

2017-04-27 00:00:00

pages

e3254

issn

2167-8359

pii

3254

journal_volume

5

pub_type

杂志文章

相关文献

PeerJ文献大全
  • Capturing patient-reported area of knee pain: a concurrent validity study using digital technology in patients with patellofemoral pain.

    abstract:Background:Patellofemoral pain (PFP) is often reported as a diffuse pain at the front of the knee during knee-loading activities. A patient's description of pain location and distribution is commonly drawn on paper by clinicians, which is difficult to quantify, report and compare within and between patients. One way of...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4406

    authors: Matthews M,Rathleff MS,Vicenzino B,Boudreau SA

    更新日期:2018-03-08 00:00:00

  • Assessment of the efficacy of using taurine supplements to improve growth and feed utilization of juvenile starry flounder (Platichthys stellatus) given diets based on soy-protein.

    abstract::A feeding trial was conducted to assess the feasibility of supplementing taurine in soy-based diets for juvenile starry flounder Platichthys stellatus. The basal diet (Crude protein 66.5%, crude lipid 8.5%) was supplemented with 0 (control), 0.5%, 1.0%, 1.5%, 2.0% and 2.5% taurine to formulate six test diets. Each die...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.10597

    authors: Li P,Bu H,Li B,Sun Y,Wang M,Song Z

    更新日期:2021-01-11 00:00:00

  • IOCBIO Sparks detection and analysis software.

    abstract::Analysis of calcium sparks in cardiomyocytes can provide valuable information about functional changes of calcium handling in health and disease. As a part of the calcium sparks analysis, sparks detection and characterization is necessary. Here, we describe a new open-source platform for automatic calcium sparks detec...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6652

    authors: Laasmaa M,Karro N,Birkedal R,Vendelin M

    更新日期:2019-03-29 00:00:00

  • Seasonal variations in leaf and branch trace elements and the influence of a 3-yr 100% rainfall exclusion on Pinus massoniana Lamb.

    abstract:Background:Trace elements are essential for the growth and survival of plants, and their concentrations and distributions in plants are effective reflections of ecological adaptation strategies. However, this aspect has seldom been addressed. Method:Changes in the leaf and branch trace elements of Pinus massoniana Lam...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9935

    authors: Lin T,Zheng X,Zheng H

    更新日期:2020-09-16 00:00:00

  • Crossmodal congruency effect scores decrease with repeat test exposure.

    abstract::The incorporation of feedback into a person's body schema is well established. The crossmodal congruency task (CCT) is used to objectively quantify incorporation without being susceptible to experimenter biases. This visual-tactile interference task is used to calculate the crossmodal congruency effect (CCE) score as ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6976

    authors: Blustein D,Gill S,Wilson A,Sensinger J

    更新日期:2019-05-22 00:00:00

  • Understanding mitochondrial myopathies: a review.

    abstract::Mitochondria are small, energy-producing structures vital to the energy needs of the body. Genetic mutations cause mitochondria to fail to produce the energy needed by cells and organs which can cause severe disease and death. These genetic mutations are likely to be in the mitochondrial DNA (mtDNA), or possibly in th...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4790

    authors: Ahuja AS

    更新日期:2018-05-21 00:00:00

  • Maxent estimation of aquatic Escherichia coli stream impairment.

    abstract:Background:The leading cause of surface water impairment in United States' rivers and streams is pathogen contamination. Although use of fecal indicators has reduced human health risk, current approaches to identify and reduce exposure can be improved. One important knowledge gap within exposure assessment is character...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5610

    authors: Gilfillan D,Joyner TA,Scheuerman P

    更新日期:2018-09-13 00:00:00

  • Are invasive populations characterized by a broader diet than native populations?

    abstract:BACKGROUND:Invasive species are among the most significant threats to biodiversity. The diet of invasive animal populations is a crucial factor that must be considered in the context of biological invasions. A broad dietary spectrum is a frequently cited characteristic of invasive species, allowing them to thrive in a ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3250

    authors: Courant J,Vogt S,Marques R,Measey J,Secondi J,Rebelo R,De Villiers A,Ihlow F,De Busschere C,Backeljau T,Rödder D,Herrel A

    更新日期:2017-05-16 00:00:00

  • Disentangling the link between supplemental feeding, population density, and the prevalence of pathogens in urban stray cats.

    abstract:Background:Supplemental feeding of free-roaming animals, including wildlife and feral or stray animals, is well known to have a substantial impact on various aspects of animal ecology including habitat use, activity patterns, and host-pathogen interactions. Among them, an increased population density (PD) of animals re...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4988

    authors: Hwang J,Gottdenker NL,Oh DH,Nam HW,Lee H,Chun MS

    更新日期:2018-06-25 00:00:00

  • Effects of stream grazers with different functional traits on the spatial heterogeneity of periphyton mats.

    abstract:Background:Grazing activity on periphytic mats determines mat structure and spatial heterogeneity. Spatial complexity in stream periphyton is highly divergent and may depend on the functional traits of the different primary consumers species (i.e., grazers) such as mouthpart morphology, feeding behavior, and feeding ac...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6747

    authors: Katano I,Doi H

    更新日期:2019-06-05 00:00:00

  • Microbiota variations in Culex nigripalpus disease vector mosquito of West Nile virus and Saint Louis Encephalitis from different geographic origins.

    abstract::Although mosquito microbiota are known to influence reproduction, nutrition, disease transmission, and pesticide resistance, the relationship between host-associated microbial community composition and geographical location is poorly understood. To begin addressing this knowledge gap, we characterized microbiota assoc...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6168

    authors: Duguma D,Hall MW,Smartt CT,Debboun M,Neufeld JD

    更新日期:2019-01-09 00:00:00

  • Regional drivers of clutch loss reveal important trade-offs for beach-nesting birds.

    abstract::Coastal birds are critical ecosystem constituents on sandy shores, yet are threatened by depressed reproductive success resulting from direct and indirect anthropogenic and natural pressures. Few studies examine clutch fate across the wide range of environments experienced by birds; instead, most focus at the small si...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2460

    authors: Maslo B,Schlacher TA,Weston MA,Huijbers CM,Anderson C,Gilby BL,Olds AD,Connolly RM,Schoeman DS

    更新日期:2016-09-13 00:00:00

  • Development and validation of a prognostic nomogram to predict overall survival and cancer-specific survival for patients with anaplastic thyroid carcinoma.

    abstract:Background:Anaplastic thyroid carcinoma (ATC) is a rare malignant tumor with a poor prognosis. However, there is no useful clinical prognostic predictive tool for ATC so far. Our study identified risk factors for survival of ATC and created a reliable nomogram to predict overall survival (OS) and cancer-specific surviv...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9173

    authors: Gui W,Zhu W,Lu W,Shang C,Zheng F,Lin X,Li H

    更新日期:2020-05-21 00:00:00

  • Prognostic analysis of histopathological images using pre-trained convolutional neural networks: application to hepatocellular carcinoma.

    abstract::Histopathological images contain rich phenotypic descriptions of the molecular processes underlying disease progression. Convolutional neural networks, state-of-the-art image analysis techniques in computer vision, automatically learn representative features from such images which can be useful for disease diagnosis, ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8668

    authors: Lu L,Daigle BJ Jr

    更新日期:2020-03-12 00:00:00

  • Distribution patterns of dinoflagellate communities along the Songhua River.

    abstract:Background:Dinoflagellates have the potential to pose severe ecological and economic damages to aquatic ecosystems. It is therefore largely needed to understand the causes and consequences of distribution patterns of dinoflagellate communities in order to manage potential environmental problems. However, a majority of ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6733

    authors: Gao Y,Chen Y,Xiong W,Li S,Zhan A

    更新日期:2019-04-09 00:00:00

  • Skin temperature and reproductive condition in wild female chimpanzees.

    abstract::Infrared thermal imaging has emerged as a valuable tool in veterinary medicine, in particular for evaluating reproductive processes. Here, we explored differences in skin temperature of twenty female chimpanzees in Budongo Forest, Uganda, four of which were pregnant during data collection. Based on previous literature...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4116

    authors: Dezecache G,Wilke C,Richi N,Neumann C,Zuberbühler K

    更新日期:2017-12-05 00:00:00

  • The nine ADAMs family members serve as potential biomarkers for immune infiltration in pancreatic adenocarcinoma.

    abstract:Background:The functional significance of ADAMs family members in the immune infiltration of pancreatic adenocarcinoma (PAAD) awaits elucidation. Methods:ADAMs family members with significant expression were identified among differentially expressed genes of PAAD based on The Cancer Genome Atlas (TCGA) database follow...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.9736

    authors: Qi B,Liu H,Dong Y,Shi X,Zhou Q,Zeng F,Bao N,Li Q,Yuan Y,Yao L,Xia S

    更新日期:2020-09-30 00:00:00

  • PKC in motorneurons underlies self-learning, a form of motor learning in Drosophila.

    abstract::Tethering a fly for stationary flight allows for exquisite control of its sensory input, such as visual or olfactory stimuli or a punishing infrared laser beam. A torque meter measures the turning attempts of the tethered fly around its vertical body axis. By punishing, say, left turning attempts (in a homogeneous env...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1971

    authors: Colomb J,Brembs B

    更新日期:2016-04-25 00:00:00

  • Water permeability/impermeability in seeds of 15 species of Caragana (Fabaceae).

    abstract::Majority legumes in the temperate and arctic zones have water-impermeable seeds (physical dormancy, PY). However, various authors have reported that seeds of some Caragana species are water-permeable and thus non-dormant. We (1) tested seeds of 15 species of Caragana matured in the same site in 2014, 2016 and/or 2017 ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.6870

    authors: Chen D,Zhang R,Baskin CC,Hu X

    更新日期:2019-05-09 00:00:00

  • Determinants of seasonal changes in availability of food patches for elephants (Loxodonta africana) in a semi-arid African savanna.

    abstract::Loss of biodiversity caused by impact of elephants (Loxodonta africana) on African woodlands may require a management response, but any action should be based on an understanding of why elephants choose to utilise trees destructively. Comprehension of elephant feeding behaviour requires consideration of the relative v...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3453

    authors: Clegg BW,O'Connor TG

    更新日期:2017-06-20 00:00:00

  • Evaluation of variation within the barcode region of Cytochrome c Oxidase I (COI) for the detection of commercial Callinectes sapidus Rathbun, 1896 (blue crab) products of non-US origin.

    abstract::Callinectes sapidus Rathbun, 1896 is a western Atlantic species with a disjointed natural geographic range from Massachusetts, USA to Venezuela (distribution area 1) and from Alagoas, Brazil to northern Argentina (distribution area 2). It is the only species of portunid crab commercially harvested in the continental U...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.7827

    authors: Windsor AM,Moore MK,Warner KA,Stadig SR,Deeds JR

    更新日期:2019-11-05 00:00:00

  • Coral larvae for restoration and research: a large-scale method for rearing Acropora millepora larvae, inducing settlement, and establishing symbiosis.

    abstract::Here we describe an efficient and effective technique for rearing sexually-derived coral propagules from spawning through larval settlement and symbiont uptake with minimal impact on natural coral populations. We sought to maximize larval survival while minimizing expense and daily husbandry maintenance by experimenta...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3732

    authors: Pollock FJ,Katz SM,van de Water JAJM,Davies SW,Hein M,Torda G,Matz MV,Beltran VH,Buerger P,Puill-Stephan E,Abrego D,Bourne DG,Willis BL

    更新日期:2017-09-06 00:00:00

  • Natural mortality estimates throughout the life history of the sea cucumber Isostichopus Badionotus (Holothuroidea: Aspidochirotida).

    abstract::The Natural Mortality coefficient (M) is a key parameter for stock assessments. The need to establish age-specific natural mortality coefficients is widely recognized because M decreases rapidly over the early stages of the life cycle until it reaches a stable M value around the age-at-maturity. The aim of this study ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5235

    authors: Romero-Gallardo S,Velázquez-Abunader I,López-Rocha JA,Garza-Gisholt E

    更新日期:2018-07-18 00:00:00

  • Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010-2018.

    abstract:Background:Human sapovirus (SaV) is an etiologic agent of acute gastroenteritis (AGE) in all age groups worldwide. Genetic recombination of SaV has been reported from many countries. So far, none of SaV recombinant strain has been reported from Thailand. This study examined the genetic recombination and genotype divers...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.8520

    authors: Kumthip K,Khamrin P,Ushijima H,Chen L,Li S,Maneekarn N

    更新日期:2020-02-06 00:00:00

  • Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates.

    abstract:Background:Bioactive peptides can prevent damage associated with oxidative stress in humans when consumed regularly. Recently, peptides have attracted immense interest because of their beneficial functional properties, safety and little or no side effects when used at high concentration. Most antioxidant peptides are s...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5337

    authors: Sonklin C,Laohakunjit N,Kerdchoechuen O

    更新日期:2018-07-27 00:00:00

  • Comparative transcriptome analysis of isonuclear-alloplasmic lines unmask key transcription factor genes and metabolic pathways involved in sterility of maize CMS-C.

    abstract::Although C-type cytoplasmic male sterility (CMS-C) is one of the most attractive tools for maize hybrid seed production, the detailed regulation network of the male sterility remains unclear. In order to identify the CMS-C sterility associated genes and/or pathways, the comparison of the transcriptomes between the CMS...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.3408

    authors: Li C,Zhao Z,Liu Y,Liang B,Guan S,Lan H,Wang J,Lu Y,Cao M

    更新日期:2017-05-30 00:00:00

  • Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells.

    abstract::Mechanical ventilation (MV) of patients can cause damage to bronchoalveolar epithelium, leading to a sterile inflammatory response, infection and in severe cases sepsis. Limited knowledge is available on the effects of MV on the innate immune defense system in the human lung. In this study, we demonstrate that cyclic ...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.1483

    authors: Karadottir H,Kulkarni NN,Gudjonsson T,Karason S,Gudmundsson GH

    更新日期:2015-12-07 00:00:00

  • Seasonal changes of commercial traits, proximate and fatty acid compositions of the scallop Flexopecten glaber from the Mediterranean Sea (Southern Italy).

    abstract::This study provides information on biological (gonadosomatic index), commercial quality (condition index and meat yield) and biochemical aspects (proximate composition, fatty acids) of the soft tissues of Flexopecten glaber reared in suspended cages in the Ionian Sea. The results showed that condition index (CI) and m...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.5810

    authors: Prato E,Biandolino F,Parlapiano I,Papa L,Denti G,Fanelli G

    更新日期:2019-01-21 00:00:00

  • A framework for smartphone-enabled, patient-generated health data analysis.

    abstract:BACKGROUND:Digital medicine and smartphone-enabled health technologies provide a novel source of human health and human biology data. However, in part due to its intricacies, few methods have been established to analyze and interpret data in this domain. We previously conducted a six-month interventional trial examinin...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.2284

    authors: Gollamudi SS,Topol EJ,Wineinger NE

    更新日期:2016-08-02 00:00:00

  • Effects of landscape features on population genetic variation of a tropical stream fish, Stone lapping minnow, Garra cambodgiensis, in the upper Nan River drainage basin, northern Thailand.

    abstract::Spatial genetic variation of river-dwelling freshwater fishes is typically affected by the historical and contemporary river landscape as well as life-history traits. Tropical river and stream landscapes have endured extended geological change, shaping the existing pattern of genetic diversity, but were not directly a...

    journal_title:PeerJ

    pub_type: 杂志文章

    doi:10.7717/peerj.4487

    authors: Jaisuk C,Senanan W

    更新日期:2018-03-07 00:00:00