Laccase-mediated crosslinking of gluten-free amadumbe flour improves rheological properties.

Abstract:

:The absence of gluten in gluten-free flours presents a challenge to their application in baking. Enzymatic modification of the protein and polysaccharides may result in a network that mimics gluten. In the current study, the effects of laccase on the rheological properties of amadumbe dough were investigated. Thiol and total phenolic contents of dough decreased by up to 28% and 93%, respectively, as laccase activity was increased (0-3 U/g flour). Both G' and G″ of laccase-treated dough increased significantly due to laccase-catalysed cross-linking of proteins and polysaccharides esterified with phenolics, as demonstrated by relevant model reactions. Tan δ decreased with increase in laccase activity indicating an increase in the elastic character of the dough. The improvement in dough viscoelasticity may enable the retention of adequate carbon dioxide during proofing and production of more acceptable gluten-free bread.

journal_name

Food Chem

journal_title

Food chemistry

authors

Manhivi VE,Amonsou EO,Kudanga T

doi

10.1016/j.foodchem.2018.05.017

subject

Has Abstract

pub_date

2018-10-30 00:00:00

pages

157-163

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(18)30807-0

journal_volume

264

pub_type

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