CTLA-4 methylation regulates the pathogenesis of myasthenia gravis and the expression of related cytokines.

Abstract:

BACKGROUND:Myasthenia gravis (MG) is a progressive autoimmune disease that occurs as a result of the failure of neuromuscular transmission and is characterized by muscle weakness. There has been evidence on the correlations between the genetic predisposition of cytotoxic T lymphocyte and the antigen-4 (CTLA-4) and MG. Thus, the present study was conducted to study is designed to examine the effects of CTLA-4 methylation on the pathogenesis of MG and the expressions of related cytokines. METHODS:The CTLA-4 methylation levels in peripheral blood were quantified in 103 samples collected from MG patients and 86 samples from healthy individiuals. The expression of serum-related cytokines as well as the Treg cell ratio were examined so as to define the contributory role of CTLA-4 methylation in MG and to identify the interaction between CTLA-4 methylation and related factors, the expressions of DNA methyltransferase (DNMT)l, DNMT3A and DNMT3B, CTLA-4, AchR-Ab, Titin-Ab, RyR-Ab, IL-2, IL-10, IFN-γ, and TGF-β, activity of P- acetylcholinesterase (AchE) and E-AchE. RESULTS:The results indicated that the incidence of CTLA-4 methylation was significantly higher in the control group when compared with the MG group, and CTLA-4 methylation was also found to be associated with the thymus status of MG patients. It was also observed from the experiment data that the expressions of DNMTl, DNMT3A, and DNMT3B, along with the expressions of AchR-Ab, Titin-Ab, RyR-Ab, IL-2, IL-10, IFN-γ and TGF-β, and the activity of P-AchE and E-AchE were all higher in the MG group than in the control group, with a reduction of CTLA-4 expression. Another key finding from this study revealed that methylation interference can lead to the suppression in the expression of AchR-Ab, the activity of E-AchE, the expression of IL-2, IL-10, IFN-γ, and TGF-β and the Treg cell ratio in lymphocytes. CONCLUSION:In conclusion, the results obtained from the present study highly indicated that CTLA-4 methylation might play a role in facilitating the occurrence of MG and increasing the expressions of related cytokines through the upregulation of AchR-Ab and E-Ach.

journal_name

Medicine (Baltimore)

journal_title

Medicine

authors

Fang TK,Yan CJ,Du J

doi

10.1097/MD.0000000000010620

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

e0620

issue

18

eissn

0025-7974

issn

1536-5964

pii

00005792-201805040-00038

journal_volume

97

pub_type

杂志文章

相关文献

MEDICINE文献大全
  • Tuberculosis-associated hemophagocytic lymphohistiocytosis with initial presentation of fever of unknown origin in a general hospital: An analysis of 8 clinical cases.

    abstract::The study aimed to investigate the clinical features and prognoses of patients with tuberculosis (TB) who had secondary hemophagocytic lymphohistiocytosis (HLH).Patients first presenting with fever of unknown origin, who were ultimately diagnosed with TB-associated secondary HLH, were assessed retrospectively. We summ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000006575

    authors: Zhang Y,Liang G,Qin H,Li Y,Zeng X

    更新日期:2017-04-01 00:00:00

  • The timing of urinary catheter removal after gynecologic surgery: A meta-analysis of randomized controlled trials.

    abstract::The present study aimed to assess the effect of removing an indwelling urinary catheter at different times on urinary retention and urinary infection in patients undergoing gynecologic surgery.Electronic databases including PubMed, EMbase, the Cochrane Central Register of Controlled Trials, and Ovid from inception to ...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析

    doi:10.1097/MD.0000000000018710

    authors: Huang H,Dong L,Gu L

    更新日期:2020-01-01 00:00:00

  • Effects of periodontal therapy on metabolic control in patients with type 2 diabetes mellitus and periodontal disease: a meta-analysis.

    abstract::Epidemiologic studies have reported increased incidence, prevalence and acuity of periodontitis in adults with diabetes and some have also suggested that treating periodontal disease may improve glycemic control in diabetic patients. This meta-analysis was conducted to evaluate the effects of different periodontal the...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析,评审

    doi:10.1097/MD.0000000000000292

    authors: Wang TF,Jen IA,Chou C,Lei YP

    更新日期:2014-12-01 00:00:00

  • Effect of Probiotics on Blood Lipid Concentrations: A Meta-Analysis of Randomized Controlled Trials.

    abstract::Previous clinical studies have reported mixed results regarding the effect of probiotics on lipid metabolism. Therefore, we conducted a meta-analysis of randomized controlled trials to quantify the direction and magnitude of the potential effect of probiotics on blood lipid concentrations.Eligible studies were randomi...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析

    doi:10.1097/MD.0000000000001714

    authors: Cho YA,Kim J

    更新日期:2015-10-01 00:00:00

  • Effects of early parenteral iron combined erythropoietin in preterm infants: A randomized controlled trial.

    abstract:BACKGROUD:The aim of the study was to evaluate the effect of early parenteral iron supplementation combined erythropoietin for prevention of anemia in preterm infants. METHODS:In total, 96 preterm infants were randomly assigned to 3 groups: a control group receiving standard parenteral nutrition (group 1: n = 31), an ...

    journal_title:Medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/MD.0000000000005795

    authors: Qiao L,Tang Q,Zhu W,Zhang H,Zhu Y,Wang H

    更新日期:2017-03-01 00:00:00

  • A systematic review of randomized controlled trials of the Wenyang Huoxue method in treating diabetic peripheral neuropathy.

    abstract:OBJECTIVE:To assess the efficacy and safety of the Wenyang Huoxue method for patients with diabetic peripheral neuropathy. METHODS:A systematic literature search was performed using 7 databases: PUBMED, EMBASE, the Chinese National Knowledge Infrastructure, Wanfang, Chinese BioMedical, and the VIP Chinese Science and ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000017618

    authors: Deng Z,Wang M,Fan YH,Huang R,Rao Y,Mai X,Liu M

    更新日期:2019-10-01 00:00:00

  • Erratum: Association of Genetic Polymorphisms on VEGFA and VEGFR2 With Risk of Coronary Heart Disease: Erratum.

    abstract::[This corrects the article DOI: 10.1097/MD.0000000000003413.]. ...

    journal_title:Medicine

    pub_type: 已发布勘误

    doi:10.1097/01.md.0000484496.43819.1a

    authors:

    更新日期:2016-08-19 00:00:00

  • Metastatic infectious disease and clinical outcome in Staphylococcus aureus and Streptococcus species bacteremia.

    abstract::Early detection of metastatic infection in patients with Gram-positive bacteremia is important as morbidity and mortality are higher in the presence of these foci, probably due to incomplete eradication of clinically silent foci during initial treatment. We performed a prospective study in 115 patients with Staphyloco...

    journal_title:Medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/MD.0b013e31824d7ed2

    authors: Vos FJ,Kullberg BJ,Sturm PD,Krabbe PFM,van Dijk APJ,Wanten GJA,Oyen WJG,Bleeker-Rovers CP

    更新日期:2012-03-01 00:00:00

  • Primary malignant mesenchymoma of bladder: Case report and review of the literature.

    abstract:RATIONALE:Malignant mesenchymoma (MM) is defined as a heterogeneous malignant soft tissue tumor that consists of 2 or more distinctly different mesenchymal components in addition to fibrosarcomatous elements. Bladder MM was rarely reported in the literature and there are only 5 cases of primary bladder MM documented in...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000007579

    authors: Yang Z,Yang X,Lu X,Gao L,Li G,Zhang X

    更新日期:2017-08-01 00:00:00

  • Experience of the management of coronary artery bypass graft only on moderate ischemic mitral regurgitation: A single-center retrospective study.

    abstract::To summary the impact of off-pump coronary artery bypass grafting (CABG) only on patients with moderate ischemic mitral regurgitation and survival.We retrospectively analyzed 109 patients with coronary artery disease (CAD) complicated by moderate mitral regurgitation, from January, 2008 to December, 2014, in the Depar...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000014969

    authors: Wang W,Li B,Wang Y,Piao H,Zhu Z,Xu R,Li D,Liu K

    更新日期:2019-04-01 00:00:00

  • Jingui Shenqi pill for nocturia due to nocturnal polyuria: A protocol for systematic review and meta-analysis.

    abstract:BACKGROUND:The incidence of nocturia is high and will seriously affect patients' physical and mental health. Nocturnal polyuria is the most critical cause of nocturia, There are few drugs currently used to treat nocturia due to Nocturnal Polyuria (NP). The guide highly recommends only Desmopressin. There is an urgent n...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析

    doi:10.1097/MD.0000000000023742

    authors: Fan Z,Wang Z,Deng J,Jiang Y

    更新日期:2020-12-18 00:00:00

  • Effectiveness and confounding factors of penetrating astigmatic keratotomy in clinical practice: Case report.

    abstract:RATIONALE:Penetrating astigmatic keratotomy (penetrating AK) is a well-known method to correct corneal astigmatism but rarely be performed nowadays. This article reevaluated the clinical effectiveness and confounding factors of penetrating AK. PATIENT CONCERNS:Penetrating AK has been introduced to serve as one alterna...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000009709

    authors: Yen CY,Tseng GL

    更新日期:2018-01-01 00:00:00

  • Serum Phosphorylated Neurofilament-Heavy Chain, a Potential Biomarker, is Associated With Peripheral Neuropathy in Patients With Type 2 Diabetes.

    abstract::Neurofilament (NF), one of the major axonal cytoskeletal proteins, plays a critical role in degenerative diseases in both the central and the peripheral nervous systems. The aim of this study is to explore the relationship between serum phosphorylated neurofilament-heavy chain (pNF-H) and diabetic peripheral neuropath...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000001908

    authors: Qiao X,Zhang S,Zhao W,Ye H,Yang Y,Zhang Z,Miao Q,Hu R,Li Y,Lu B

    更新日期:2015-11-01 00:00:00

  • Recurrent meningococcal meningitis with complement 6 (C6) deficiency: A case report.

    abstract:RATIONALE:Late complement deficiency increases susceptibility to meningococcal disease and recurrent infections. In Korea, 5 case reports have described meningococcal disease with complement deficiency. However, C6 deficiency has not been described previously. PATIENT CONCERNS:A 21-year-old police trainee presented wi...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000020362

    authors: Bae JY,Ham A,Choi HJ,Kim CJ

    更新日期:2020-05-22 00:00:00

  • Accuracy of objective parameters in acute epiglottitis diagnosis: A case-control study.

    abstract::Lateral neck radiography is often used as a screening tool in emergency departments for suspected acute epiglottitis. The qualitative radiographic signs have been mainly used. The aim of this study was to evaluate the accuracy of objective radiographic parameters to aid diagnosis of acute epiglottitis.Patients who wer...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000012256

    authors: Kim KH,Kim YH,Lee JH,Lee DW,Song YG,Cha SY,Hwang SY

    更新日期:2018-09-01 00:00:00

  • The association between age and adverse events due to biologic disease-modifying antirheumatic drugs in patients with rheumatoid arthritis: A retrospective cohort study.

    abstract:ABSTRACT:We examined whether the age of patients with rheumatoid arthritis was associated with adverse events (AEs) caused by biologic disease-modifying antirheumatic drugs (bDMARDs).Patients with rheumatoid arthritis using bDMARDs from Showa University Hospital, Showa University Northern Yokohama Hospital, and Showa U...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000023861

    authors: Ikari Y,Yajima N,Miwa Y

    更新日期:2020-12-24 00:00:00

  • Restrictive allogeneic blood transfusion strategy in patients with extremity bone sarcomas.

    abstract::Allogeneic blood transfusions (ABTs) are common in patients with cancer. The present study investigated the safety of a restrictive ABT strategy in patients with extremity sarcomas.Patients who underwent operations for extremity bone sarcomas between May 2008 and November 2018 were retrospectively reviewed. Clinical o...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000018482

    authors: Park JH,Hong SH,Jang WY

    更新日期:2019-12-01 00:00:00

  • Novel strength test battery to permit evidence-based paralympic classification.

    abstract::Ordinal-scale strength assessment methods currently used in Paralympic athletics classification prevent the development of evidence-based classification systems. This study evaluated a battery of 7, ratio-scale, isometric tests with the aim of facilitating the development of evidence-based methods of classification. T...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000000031

    authors: Beckman EM,Newcombe P,Vanlandewijck Y,Connick MJ,Tweedy SM

    更新日期:2014-06-01 00:00:00

  • Penetrating neck trauma with common carotid artery injury caused by a percussive drill: A case report.

    abstract:RATIONALE:Penetrating neck traumas are dangerous and have a high mortality rate, particularly in patients with common carotid artery injuries. Advances in diagnostic imaging technology have shifted management of penetrating neck injuries from mandatory exploration to selective management. The question is now regarding ...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000015750

    authors: Wang D,Zhao Y,Cha B,Fang P,Liu Y

    更新日期:2019-05-01 00:00:00

  • Association between early Helicobacter pylori eradication and a lower risk of recurrent complicated peptic ulcers in end-stage renal disease patients.

    abstract::End-stage renal disease (ESRD) patients exhibit an increased incidence of peptic ulcer disease. Helicobacter pylori plays a central role in the development of peptic ulcers. The effect of early H pylori eradication on the recurrence of complicated peptic ulcer disease in ESRD patients remains unclear. The aim of the p...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000000370

    authors: Chang SS,Hu HY

    更新日期:2015-01-01 00:00:00

  • Various clinical features of patients with anti-Hu associated paraneoplastic neurological syndromes: An observational study.

    abstract::To describe and analyze the clinical features and prognosis of patients with anti-Hu associated paraneoplastic neurological syndromes (PNS).The symptoms, MRI findings, cerebrospinal fluid (CSF) changes, electroencephalogram (EEG) characteristics and prognoses of 9 well-diagnosed anti-Hu associated PNS patients were an...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000010649

    authors: Li J,Lin W

    更新日期:2018-05-01 00:00:00

  • Primary pulmonary hepatoid adenocarcinoma: A case report and review of the literature.

    abstract:RATIONALE:Hepatoid adenocarcinoma of lung (HAL) is a rare malignant tumor, which can be defined as a primary alpha-fetoprotein (AFP)-producing lung carcinoma. The majority of hepatoid adenocarcinoma (HAC) expressed AFP in tumor cells, but AFP expression is not required for its diagnosis according to the modified diagno...

    journal_title:Medicine

    pub_type: 杂志文章,评审

    doi:10.1097/MD.0000000000015053

    authors: Yang K,Jiang H,Li Q

    更新日期:2019-04-01 00:00:00

  • Perioperative Blood Glucose Levels 

    abstract::Hyperglycemia is common during and after Coronary Artery Bypass Graft Surgery (CABGS) and has been shown to be associated with poor clinical outcomes. In this study, we hypothesized that a moderate perioperative mean blood glucose level of <150 mg/dL improves long-term survival in cardiac surgery patients. We conducte...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000002035

    authors: Mansur A,Popov AF,Hanna AA,Bergmann I,Brandes IF,Beissbarth T,Bauer M,Hinz J

    更新日期:2015-11-01 00:00:00

  • Risk of sexually transmitted infections following depressive disorder: A nationwide population-based cohort study.

    abstract::Depressive disorder is a severe mental disorder associated with functional and cognitive impairment. Numerous papers in the literature investigated associations between sexually transmitted infections (STIs) and psychiatric illnesses. However, the results of these studies are controversial.We explored the relationship...

    journal_title:Medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1097/MD.0000000000012539

    authors: Huang SY,Hung JH,Hu LY,Huang MW,Lee SC,Shen CC

    更新日期:2018-10-01 00:00:00

  • Risk Factors for Diabetes Mellitus in Chronic Pancreatitis: A Cohort of 2,011 Patients.

    abstract::Diabetes mellitus (DM) is a common complication of chronic pancreatitis (CP) and increases the mortality. The identification of risk factors for DM development may contribute to the early detection and potential risk reduction of DM in patients with CP.Patients with CP admitted to Changhai Hospital (Shanghai, China) f...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000003251

    authors: Pan J,Xin L,Wang D,Liao Z,Lin JH,Li BR,Du TT,Ye B,Zou WB,Chen H,Ji JT,Zheng ZH,Hu LH,Li ZS

    更新日期:2016-04-01 00:00:00

  • Correlation between self-perceived burden and self-management behavior in elderly stroke survivors: A longitudinal observational study.

    abstract::The aim of this study was to assess the levels of self-perceived burden (SPB) and self-management behavior in elderly stroke survivors during the first 3 months after acute stroke, and to explore the correlation between them.A total of 203 consecutive hospitalized elderly patients diagnosed with stroke were recruited....

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000022862

    authors: Ren XR,Wei YY,Su XN,Hua Y,Shao P,Xiao T,Wang J,Ni CP,Guo HX

    更新日期:2020-10-30 00:00:00

  • HIV prevalence and sexual behavior among young male conscripts in the Brazilian army, 2016.

    abstract::Since 1996, the Brazilian Department of Sexually Transmitted Infections (STI), AIDS and Viral Hepatitis (Departamento de Vigilância, Prevenção e Controle das IST, do HIV, AIDS e Hepatites Virais, Secretaria de Vigilância em Saúde, Ministério da Saúde) in collaboration with the Brazilian Ministry of Defense has conduct...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000009014

    authors: Sperhacke RD,da Motta LR,Kato SK,Vanni AC,Paganella MP,Oliveira MCP,Pereira GFM,Benzaken AS

    更新日期:2018-05-01 00:00:00

  • Osteonecrosis of the femoral head, nonunion and potential risk factors in Pauwels grade-3 femoral neck fractures: A retrospective cohort study.

    abstract::The present study was to analyze clinical outcome of Pauwels grade-3 femoral neck fractures treated by different surgical techniques. Potential risk factors associated with nonunion and osteonecrosis of the femoral head (ONFH) were investigated as well. The retrospective study comprised of 67 sequential patients treat...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000003706

    authors: Zhang YL,Chen S,Ai ZS,Gao YS,Mei J,Zhang CQ

    更新日期:2016-06-01 00:00:00

  • Milk and yogurt intake and breast cancer risk: A meta-analysis.

    abstract::Different patterns of milk and breast cancer rates across countries suggest that several components of milk could affect breast cancer risk. However, the components of diet are complex including milk that could potentially influence risk. Some milk products such as whole milk and cheese have a high fat content which m...

    journal_title:Medicine

    pub_type: 杂志文章,meta分析

    doi:10.1097/MD.0000000000014900

    authors: Chen L,Li M,Li H

    更新日期:2019-03-01 00:00:00

  • Case report: A non-obstructive azoospermia patient with heat shock factor-2 mutation.

    abstract:RATIONALE:Infertility is a common medical condition that affects nearly 15% of the world population. Non-obstructive azoospermia (NOA) is one of the most severe forms of male infertility. Some common structural variants, single nucleotide polymorphisms (SNPs), and genetic factors were reported to be associated with NOA...

    journal_title:Medicine

    pub_type: 杂志文章

    doi:10.1097/MD.0000000000021107

    authors: Zhao H,Zhang H,Xi Q,Li L,Zhu H,Hu X,Liu R

    更新日期:2020-07-31 00:00:00