Abstract：
:Lack of regulated expression and tissue specificity are the major drawbacks of plant and virus-derived constitutive promoters. A precise tissue or site-specific expression, facilitate regulated expression of proteins at the targeted time and site. Publically available microarray data on whitefly and aphid infested Arabidopsis thaliana L. was used to identify whitefly and aphid-inducible genes. The qRT-PCR further validated the inducible behaviour of these genes under artificial infestation. Promoter sequences of genes were retrieved from the Arabidopsis Information Resources database with their corresponding 5'UTR and cloned from the A. thaliana genome. Promoter reporter transcriptional fusions were developed with the beta-glucuronidase (GUS) gusA gene in a binary expression vector to validate the inducible behaviour of these promoters in eight independent transgenic Nicotiana tabaccum lines. Histochemical analysis of the reporter gene in T2 transgenic tobacco lines confirmed promoter driven expression at the sites of aphid and whitefly infestation. The qRT-PCR and GUS expression analysis of transgenic lines revealed that abscisic acid largely influenced the expression of both aphid and whitefly inducible promoters. Further, whitefly-specific promoter respond to salicylic acid and jasmonic acid (JA), whereas aphid-specific promoters to JA and 1-aminocyclopropane carboxylic acid. The response of promoters to phytohormones correlated to the presence of corresponding conserved cis-regulatory elements.
journal_name
J Genetjournal_title
Journal of geneticsauthors
Dubey NK,Mishra DK,Idris A,Nigam D,Singh PK,Sawant SVsubject
Has Abstractpub_date
2018-03-01 00:00:00pages
109-119issue
1eissn
0022-1333issn
0973-7731journal_volume
97pub_type
杂志文章abstract：:Rattans, the spiny climbing palms of Arecaceae (Palmae) family exhibit high endemism to the biodiversity hot spots in India. Of the five rattan genera, Calamus is the only genus found in peninsular India with 15 of 21 species, endemic to the Western Ghats. The extensive utilization of rattans owing to their strength, ...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2019-11-01 00:00:00
abstract：:Cupressus gigantea and C. torulosa are ecologically and economically important endemic species of the conifer family Cupressaceae on the Qinghai-Tibetan plateau. C. gigantea was previously classified as a subspecies of C. torulosa because of their similar morphological characteristics and close distribution. In this s...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2019-03-01 00:00:00
abstract：:Garcinia gummi-gutta (L.) Roxb. (Clusiaceae) is an endemic, semidomesticated, fruit-yielding tree species distributed in the Western Ghats of India and Sri Lanka. Various bioactive phytochemicals, such as garcinol, benzophenones and xanthones are isolated from G. gummi-gutta and have shown antibacterial, antiviral and...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-017-0756-0
更新日期：2017-06-01 00:00:00
abstract：:We studied nucleotide sequence variation at the gene coding for dopa decarboxylase (Ddc) in seven populations of Drosophila melanogaster. Strength and pattern of linkage disequilibrium are somewhat distinct in the extensively sampled Spanish and Raleigh populations. In the Spanish population, a few sites are in strong...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-007-0017-8
更新日期：2007-08-01 00:00:00
abstract：:Cottonseed contains 16% seed oil and 23% seed protein by weight. High levels of palmitic acid provides a degree of stability to the oil, while the presence of bound gossypol in proteins considerably changes their properties, including their biological value. This study uses genetic principles to identify genomic regio...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-015-0489-x
更新日期：2015-03-01 00:00:00
abstract：:The ad-8 gene of Neurospora crassa, in addition to being used for the study of purine biology, has been extensively studied as a model for gene structure, mutagenesis and intralocus recombination. Because of this there is an extensive collection of well-characterized N. crassa ad-8 mutants in the Fungal Genetics Stock...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-012-0175-1
更新日期：2012-08-01 00:00:00
abstract：:Negative supercoiling stimulates transcription of many genes. In contrast, transcription of the genes coding for DNA gyrase is subject to a novel mechanism of autoregulation, wherein relaxation of the template DNA stimulates their transcription. Since DNA gyrase is the sole supercoiling activity in the eubacterial cel...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/BF02717907
更新日期：2001-12-01 00:00:00
abstract：:Multiple experimental evolution studies on Drosophila melanogaster in the 1980s and 1990s indicated that enhanced competitive ability evolved primarily through increased larval tolerance to nitrogenous wastes and increased larval feeding and foraging rate, at the cost of efficiency of food conversion to biomass, and t...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-016-0656-8
更新日期：2016-09-01 00:00:00
abstract：:We present evidence for coexistence of three different Drosophila species by rescheduling their life history traits in a natural population using the same resource, at the same time and same place. D. ananassae has faster larval development time (DT) and faster DT(egg-fly) than other two species thus utilizing the res...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/BF02715798
更新日期：2005-12-01 00:00:00
abstract：:miRNAs are important regulators of plant gene expression. There are few studies on the regulation of miRNAs in Lonicera edulis. We used high-throughput sequencing technology to analyse miRNAs in L. edulis, aiming to identify miRNAs and elucidate their function in L. edulis. In the present study, we employed the high-t...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
abstract：:While much of our understanding of genetic inheritance is based on the genome of the organism, it is becoming clear that there is an ample amount of epigenetic inheritance, which though reversible, escapes erasing process during gametogenesis and goes on to the next generation. Several examples of transgenerational in...
journal_title：Journal of genetics
pub_type： 杂志文章,评审
doi：10.1007/s12041-017-0788-5
更新日期：2017-07-01 00:00:00
abstract：:Proline and glutamine-rich wheat seed endosperm proteins are collectively referred to as prolamins. They are comprised of HMW-GSs, LMW-GSs and gliadins. HMW-GSs are major determinants of gluten elasticity and LMW-GSs considerably affect dough extensibility and maximum dough resistance. The inheritance of glutenin subu...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-010-0025-y
更新日期：2010-08-01 00:00:00
abstract：:Genomewide association study (GWAS) is an efficient tool for the detection of SNPs and candidate genes in quantitative traits. Growth rate is an important trait for increasing the meat production in sheep. A total of 96 Baluchi sheep were genotyped using Illumina Ovine SNP50 BeadChip to run a GWAS for an average daily...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2018-06-01 00:00:00
abstract：:We describe a newborn female with a de novo duplication of chromosomes 2q31.2 and 2q37.3, and a de novo monosomy 9p24.3. The clinical findings of this patient include congenital heart defects, dysmorphic facial features, hypotonia, feeding difficulties and microcephaly. Ultrasonographic prenatal findings were negative...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
abstract：:The development of the DNA theory of inheritance culminated in the publication of the molecular structure of DNA 60 years ago. This paper describes this development, beginning with the discovery of DNA as a chemical substance by Friedrich Miescher in 1869, followed by its basic chemical analysis and demonstration of i...
journal_title：Journal of genetics
pub_type： 历史文章,杂志文章,评审
doi：10.1007/s12041-014-0337-4
更新日期：2014-04-01 00:00:00
abstract：:Association mapping is a powerful high-resolution mapping tool for complex traits. The objective of this study was to identify QTLs for partial resistance to Phytophthora sojae. In this study, we evaluated a total of 214 soybean accessions by the hypocotyl inoculation method, and 175 were susceptible. The 175 suscepti...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-014-0383-y
更新日期：2014-08-01 00:00:00
abstract：:Amphibian secretion is an important source of bioactive molecules that naturally protect the skin against noxious microorganisms. Collectively called antimicrobial peptides (AMPs), these molecules have a wide spectrum of action, targeting viruses, bacteria and fungi. Like many membrane and secreted proteins, AMPs have...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2018-12-01 00:00:00
abstract：:Glutathione transferases (GSTs; EC 2.5.1.18) play important roles in stress tolerance and metabolic detoxification in plants.In higher plants, studies on GSTs have focussed largely on agricultural plants. There is restricted information about molecular characterization of GSTs in gymnosperms. To date, only tau class G...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-015-0538-5
更新日期：2015-09-01 00:00:00
abstract：:To understand the phylogenetic position of Bostrychus sinensis in Eleotridae and the phylogenetic relationships of the family, we determined the nucleotide sequence of the mitochondrial (mt) genome of Bostrychus sinensis. It is the first complete mitochondrial genome sequence of Bostrychus genus. The entire mtDNA sequ...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-013-0259-6
更新日期：2013-01-01 00:00:00
abstract：:Here, we describe a rapid, efficient and noncomplicated method for extracting poultry genomic DNA. This simple method obtains excellent qualities of molecular biology grade DNA in a matter of only about 15 min. A straightforward protocol is followed in this extraction procedure, in which, when the blood cells are plac...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2018-08-24 00:00:00
abstract：:Eucalyptus is an important short rotation pulpy woody plant, grown widely in the tropics. Recently, many genomic programmes are underway leading to the accumulation of voluminous genomic and expressed sequence tag sequences in public databases. These sequences can be utilized for analysis of simple sequence repeats (S...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-008-0003-9
更新日期：2008-04-01 00:00:00
abstract：:The aetiology of developmental dyslexia (DD) is complex; although candidate genes have been suggested, the molecular mechanism and risk factors remain unknown. The KIAA0319 gene is functionally related to neuronal migration and axon growth, and several studies have examined associations between KIAA0319 polymorphisms ...
journal_title：Journal of genetics
pub_type： 杂志文章,meta分析
doi：
更新日期：2019-06-01 00:00:00
abstract：:The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of t...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-014-0351-6
更新日期：2014-04-01 00:00:00
abstract：:The deep divergence of mammalian groups 166 and 190 million years ago (MYA) provide genetic variation to explore the evolution of DNA sequence, gene arrangement and regulation of gene expression in mammals. With encouragement from the founder of the field, Mary Lyon, techniques in cytogenetics and molecular biology we...
journal_title：Journal of genetics
pub_type： 杂志文章,评审
doi：10.1007/s12041-015-0572-3
更新日期：2015-12-01 00:00:00
abstract：:The Drosophila simulans Lhr rescues lethal hybrids from the cross of D. melanogaster and D. simulans. We describe here, the phenotypes of Lhr dependent rescue hybrids and demonstrate the effects of Lhr on functional morphology of the salivary chromosomes in the hybrids. Our results reveal that the phenotypes of the 'L...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-007-0028-5
更新日期：2007-12-01 00:00:00
abstract：:Low temperature stress is one of the major limiting factors affecting rice productivity in higher altitudes. DREB1A and DREB1B, are two transcription factors that have been reported to play key regulatory role in low temperature tolerance. In order to understand whether natural genetic variation in these two loci lead...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-015-0507-z
更新日期：2015-06-01 00:00:00
abstract：:Somatic embryos (SE) of habanero pepper (Capsicum chinense Jacq.) represent persistent deformations in the shoot apical meristem (SAM), which inhibits their capacity to form organs and subsequently plants. In dicotyledonous plants, SAM is formed in the apex, between cotyledons and it plays a central role in postembryo...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：
更新日期：2019-09-01 00:00:00
abstract：:Central Himalayan region of India encompasses varied ecological habitats ranging from near tropics to the mid-elevation forests dominated by cool-temperate taxa. In past, we have reported several new records and novel species from Uttarakhand state of India. Here, we assessed genetic variations in three mitochondrial ...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-016-0646-x
更新日期：2016-06-01 00:00:00
abstract：:It is not clear whether matK evolves under Darwinian selection. In this study, the gymnosperm Taxaceae, Cephalotaxaceae and Pinaceae were used to illustrate the physicochemical evolution, molecular adaptation and evolutionary dynamics of gene divergence in matKs. matK sequences were amplified from 27 Taxaceae and 12 C...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/s12041-010-0014-1
更新日期：2010-04-01 00:00:00
abstract：:Some members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes, the her6 gene, a member of the HES family, is the homologue of hes1 in mammals and chicken. In this study, the her6 gene and its...
journal_title：Journal of genetics
pub_type： 杂志文章
doi：10.1007/BF02935327
更新日期：2006-12-01 00:00:00
