Abstract：
:MiR-216a-5p has been acknowledged as an oncogene and is known to be involved in the progression and metastasis of numerous cancer subtypes. However, the potential role of miR-216a-5p in renal cell carcinoma (RCC) remains to be elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction was performed to detect the expression levels of miR-216a-5p in RCC tissues. Cell counting kit-8, MTT, wound scratch, Transwell and flow cytometric assays were performed to establish the biological functions of miR-216a-5p in RCC. Functional experiments demonstrated that the expression of miR-216a-5p was upregulated in RCC (P<0.05) and miR-216a-5p mimics promoted cellular proliferation, viability and motility, and suppressed apoptosis. Conversely, miR-216a-5p inhibitor suppressed cellular proliferation, viability, motility and induced apoptosis. Based on these findings, it was concluded that miR-216a-5p may function as an oncogene in RCC. MiR-216a-5p target genes need to be explored and the potential of miR-216a-5p to be used as a diagnostic or a prognostic biomarker for RCC needs to be validated by future research.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Chen P,Quan J,Jin L,Lin C,Xu W,Xu J,Guan X,Chen Z,Ni L,Yang S,Chen Y,Lai Ydoi
10.3892/etm.2018.5881subject
Has Abstractpub_date
2018-04-01 00:00:00pages
4039-4046issue
4eissn
1792-0981issn
1792-1015pii
ETM-0-0-5881journal_volume
15pub_type
杂志文章abstract：:Atherosclerosis is the leading cause of death from vascular diseases worldwide, and endothelial cell (EC) dysfunction is the key cause of atherosclerosis. miR-155 was found to induce endothelial injury and to trigger atherosclerosis. In addition, brain and muscle ARNT-like protein-1 (Bmal1) has been found to be closel...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9259
更新日期：2020-12-01 00:00:00
abstract：:The therapeutic effect of saikosaponin a (SSa) on hyperlipidemic pancreatitis (HP) is not completely understood. The aim of the present study was to investigate the therapeutic effect and the underlying mechanism of SSa using a rat model of HP. Following successful establishment of the HP rat model, different doses of...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.8324
更新日期：2020-02-01 00:00:00
abstract：:Schwannomas may arise from any peripheral nerve containing Schwann cells. However, sural nerve schwannoma is extremely rare. In this study, a case of solitary schwannoma originating from the sural nerve in a 42-year-old male is presented. Physical examination revealed a 3-cm, elastic-hard, mobile, non-tender mass, whi...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2013.1395
更新日期：2014-01-01 00:00:00
abstract：:Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are an effective treatment for advanced non-small cell lung cancer. The objective of the present study was to compare the efficacy of gefitinib and erlotinib in patients with pulmonary adenocarcinoma, whose tumor EGFR mutation status was known. Pu...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2011.383
更新日期：2012-02-01 00:00:00
abstract：:The aim of the present study was to investigate the effect of Forkhead family transcription factor P3 (Foxp3) knockdown on the function of cluster of differentiation (CD)4+CD25+ regulatory T cell (Tregs) and the tumor growth of a hepatocellular carcinoma (HCC) mouse model. CD4+CD25+ Tregs and CD4+CD25- T cells were so...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5421
更新日期：2018-01-01 00:00:00
abstract：:Sepsis is a serious condition characterized by an infectious process that induces a severe systemic inflammatory response. In this study, the effects of gemfibrozil (GFZ) on the inflammatory response associated with abdominal sepsis were investigated using a rat model of cecal-ligation and puncture (CLP). Male Wistar ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2015.2190
更新日期：2015-03-01 00:00:00
abstract：:The aim of this study was to validate the feasibility of modified frontolateral partial laryngectomy without tracheotomy using animal experiments. The glottic area before and after surgery of 6 excised canine larynges and 10 canine larynges in vivo were compared to observe whether the glottic area following modified f...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2012.838
更新日期：2013-02-01 00:00:00
abstract：:The aim of the present study was to summarize the clinical characteristics of nocardiosis caused by Nocardia otitidiscaviarum in order to improve the knowledge of nocardiosis. A case of dissemination nocardiosis caused by N. otitidiscaviarum in an immunocompetent host is reported and the associated literature reviewed...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.3755
更新日期：2016-11-01 00:00:00
abstract：:The aim of the present study was to evaluate and compare the effects of single- and two-dose low-level laser therapy (LLLT) on the postoperative swelling, trismus and pain of patients undergoing extraction of impacted mandibular third molars. In addition, edema was volumetrically measured with a 3dMD face system. A to...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.5921
更新日期：2018-04-01 00:00:00
abstract：:Multi infarct dementia (MID) is a form of dementia that is preventable and treatable. However, at present, the drugs used in MID treatment were developed for Alzheimer's disease. While only a limited range of drugs is available, the incidence of MID is increasing year on year. The present study aimed to investigate th...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9198
更新日期：2020-11-01 00:00:00
abstract：:The present study evaluated the association between different Mycoplasma pneumoniae (M. pneumonia) genotypes and clinical features of pediatric patients. Subjects were children diagnosed with community-acquired pneumonia at the Children's Hospital of Soochow University (Suzhou, China) from January 2012 to December 201...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.4721
更新日期：2017-09-01 00:00:00
abstract：:To study the effects of multiple cerebral concussion (MCC)-induced chronic traumatic encephalopathy on balance motion behavior learning and memory abilities of rats and its possible mechanism. 4MCC rat models were established by means of striking the head (4MCC group, n=15), while normal Sprague-Dawley (SD) rats were ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6474
更新日期：2018-09-01 00:00:00
abstract：:[This corrects the article DOI: 10.3892/etm.2016.3317.]. ...
journal_title：Experimental and therapeutic medicine
pub_type： 已发布勘误
doi：10.3892/etm.2016.3488
更新日期：2016-09-01 00:00:00
abstract：:Coronary artery disease (CAD) is a leading cause of death, and microRNAs (miRNAs) are widely involved in physiological and pathological processes of CAD. We chose the targetscore method calculated via the variational Bayesian Gaussian mixture model (VB-GMM) as the prediction method of target genes. By observing the de...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2019.7195
更新日期：2019-03-01 00:00:00
abstract：:Embelin is a small-molecule inhibitor extracted from plants of the Myrsinaceae family demonstrating specific inhibition of the X-linked inhibitor of apoptosis protein (XIAP) to affect the proliferation and apoptosis of various types of tumor cells. However, the mechanism of action for this effect remains unclear. The ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2012.637
更新日期：2012-10-01 00:00:00
abstract：:Schwann cell proliferation and differentiation is critical for the remyelination of injured peripheral nerves. Ferulic acid (FA) is a widely used antioxidant agent with neuroprotective properties. However, the potentially beneficial effects of FA on Schwann cells are unknown. Therefore, the present study was designed ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.3525
更新日期：2016-09-01 00:00:00
abstract：:This study aimed to assess the effect on solid tumors of mild temperature hyperthermia (MTH) combined with hexamethylenetetramine (HMTA) or tirapazamine (TPZ). Squamous cell carcinoma (SCC VII) tumor-bearing mice were continuously administered 5-bromo-2'-deoxyuridine (BrdU) to label intratumor proliferating (P) cells....
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm_00000027
更新日期：2010-01-01 00:00:00
abstract：:The complex network of etiological factors, signals and tissue responses involved in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) cannot be successfully targeted by a single therapeutic agent. Multimodal approaches to the therapy of CP/CPPS have been and are currently being tested, as in the frame of com...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2014.2152
更新日期：2015-03-01 00:00:00
abstract：:[This corrects the article DOI: 10.3892/etm.2015.2860.]. ...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章,已发布勘误
doi：10.3892/etm.2019.7267
更新日期：2019-04-01 00:00:00
abstract：:Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida. The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS. The optimal processing conditions were as fo...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.8720
更新日期：2020-07-01 00:00:00
abstract：:Acyclovir has been a frequently used antiviral agent in the clinical treatment of leukemia, acute encephalitis, malignant tumor and herpes simplex. The adverse effects of this drug have been widely described in clinical practice. In the present study, a case of a 35-year-old female patient diagnosed with herpes simple...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2016.3905
更新日期：2016-12-01 00:00:00
abstract：:Serum thymidine kinase 1 (STK1) is a reliable proliferation marker in most solid tumors, including gastric cancer. The aim of this study was to evaluate whether STK1 levels are related to the tumor response to chemotherapy and survival in advanced gastric cancer. The results showed that the average STK1 level in patie...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2011.338
更新日期：2011-11-01 00:00:00
abstract：:Thalidomide (THD) exhibits antitumor effects in several types of cancer. However, the failure of THD to inhibit tumor growth has also been observed in a number of murine models in vivo. The mechanism involved in the therapeutic failure of THD remains unclear. The present study demonstrated that, accompanied by growth-...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.5028
更新日期：2017-11-01 00:00:00
abstract：:Schisandrin B (SchB) is one of the primary active components of Schisandra chinensis (Turcz.) Baill., a traditional Chinese herb that has been used to treat insomnia for hundreds of years. Our previous studies revealed that SchB exerts sedative and hypnotic effects, increasing the content of γ-aminobutyric acid (GABA)...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2020.9271
更新日期：2020-12-01 00:00:00
abstract：:17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) is a water soluble, semisynthetic derivative of endotoxin that has anticancer effects. The aim of the present study was to determine whether 17-DMAG enhances the apoptosis of lymphoma cells in diffuse large B-cell lymphoma. Apoptosis was induced in SU-DHL-4...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2017.4995
更新日期：2017-10-01 00:00:00
abstract：:Cyclic adenosine monophosphate (cAMP) is an important secondary messenger that has long been recognized to control the initiation of meiosis through the activation of protein kinase A (PKA) in mammalian oocytes. However, PKA is not the only target for cAMP. Recent studies on cAMP-dependent and PKA-independent pathways...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.6253
更新日期：2018-08-01 00:00:00
abstract：:Limited data exist concerning the long-term (≥5 year) survival rates of patients with stage IIIB and IV non-small cell lung carcinoma (NSCLC) receiving chemotherapy. We aimed to determine the long-term results of cisplatin plus third-generation (vinorelbine or gemcitabine) cytotoxic chemotherapy in patients with local...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2012.714
更新日期：2012-12-01 00:00:00
abstract：:The functions of telomeric repeat-binding factor 1 (TRF1) and 2 (TRF2) in oral carcinogenesis are largely unexplored. This study examined the relationship between the expression of TRF1 and TRF2 and clinicopathological variables and survival in oral cavity squamous cell carcinoma (OCSCC). Western blotting and immunohi...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2010.168
更新日期：2011-01-01 00:00:00
abstract：:The herbal medicine Yin Huang Mixture (YHHJ; patent no. 200910031240.7) is an aqueous extract composed from various herbs, including Artemisia capillaries Thunb, Hypericum japonicum Thunb, Eucommia ulmoides Oliver, Rheum officinale Baill, Gardenia jasminoides Ellis, Poria cocos Wolf and Dictamnus dasycarpus Turcz. Pre...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2018.5891
更新日期：2018-04-01 00:00:00
abstract：:The present study was designed to evaluate the anti-fatigue activity and the behavioral and biochemical effects of Kai-Xin-San (KXS) extracts on fatigued rats. The rats were randomly divided into six groups: untreated control (UC), running control (RC), RC treated with 13 mg/kg/day modafinil and RC treated with KXS at...
journal_title：Experimental and therapeutic medicine
pub_type： 杂志文章
doi：10.3892/etm.2013.1256
更新日期：2013-10-01 00:00:00
