Abstract：
:How the brain makes trillions of synaptic connections using a genome of only 20,000 genes is a major question in modern neuroscience. Alternative splicing is one mechanism that can increase the number of proteins produced by each gene, but its role in regulating synapse formation is poorly understood. In Drosophila, photoreceptors form a synapse with multiple postsynaptic elements including lamina neurons L1 and L2. L1 and L2 express distinct isoforms of the homophilic repulsive protein Dscam2, and since these isoforms cannot bind to each other, cell-specific expression has been proposed to be necessary for preventing repulsive interactions that could disrupt the synapse. Here, we show that the number of synapses are reduced in flies that express only one isoform, and L1 and L2 dendritic morphology is perturbed. We propose that these defects result from inappropriate interactions between L1 and L2 dendrites. We conclude that regulated Dscam2 alternative splicing is necessary for the proper assembly of photoreceptor synapses.
journal_name
Geneticsjournal_title
Geneticsauthors
Kerwin SK,Li JSS,Noakes PG,Shin GJ,Millard SSdoi
10.1534/genetics.117.300432subject
Has Abstractpub_date
2018-02-01 00:00:00pages
717-728issue
2eissn
0016-6731issn
1943-2631pii
genetics.117.300432journal_volume
208pub_type
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