Abstract:
:15-Lipoxygenase-1 (15-LO-1) is involved in many pathological processes. The purpose of this study was to determine the potential role of 15-LO-1 in osteoarthritis (OA). The levels of 15-LO-1 expression were measured by western blotting and quantitative real-time PCR in articular cartilage from the OA rat models and OA patients. To further investigate the effects of 15-LO-1 on chondrocyte functions, such as extracellular matrix (ECM) secretion, the release of matrix-degrading enzymes, the production of reactive oxygen species (ROS), cell proliferation and apoptosis, we decreased or increased 15-LO-1 expression in chondrocytes by means of transfecting with siRNA targeting 15-LO-1 and plasmid encoding 15-LO-1, respectively. The results showed that 15-LO-1 expression was obviously increased in articular cartilage from OA rats and OA patients. It was also found that many factor-related OA, such as mechanical loading, ROS, SNP and inflammatory factor, significantly promoted 15-LO-1 expression and activity in chondrocytes. Silencing 15-LO-1 was able to markedly alleviate mechanical loading-induced cartilage ECM secretion, cartilage-degrading enzyme secretion and ROS production. Overexpression of 15-LO-1 could inhibit chondrocyte proliferation and induce chondrocyte apoptosis. In addition, reduction of 15-LO-1 in vivo significantly alleviated OA. Taken together, these results indicate that 15-LO-1 has an important role in the disease progression of OA. Thus 15-LO-1 may be a good target for developing drugs in the treatment of OA.
journal_name
Cell Death Disjournal_title
Cell death & diseaseauthors
Chen K,Yan Y,Li C,Yuan J,Wang F,Huang P,Qian N,Qi J,Zhou H,Zhou Q,Deng L,He C,Guo Ldoi
10.1038/cddis.2017.511subject
Has Abstractpub_date
2017-10-12 00:00:00pages
e3109issue
10issn
2041-4889pii
cddis2017511journal_volume
8pub_type
杂志文章abstract::The anti-apoptotic protein MCL-1 is a key regulator of cancer cell survival and a known resistance factor for small-molecule BCL-2 family inhibitors such as ABT-263 (navitoclax), making it an attractive therapeutic target. However, directly inhibiting this target requires the disruption of high-affinity protein-protei...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.561
更新日期:2015-01-15 00:00:00
abstract::Wallerian degeneration is an active program tightly associated with axonal degeneration, required for axonal regeneration and functional recovery after nerve damage. Here we provide a functional molecular foundation for our undertstanding of the complex non-cell autonomous role of glial cells in the regulation of axon...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2017.489
更新日期:2017-11-09 00:00:00
abstract::Spermidine, a natural polyamine presented widely in mammalian cells, has been implicated to extend the lifespan of several model organisms by inducing autophagy. However, the effect of spermidine against neuronal damage has not yet been fully determined. In this study, neuronal cell injury was induced by treating PC12...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2017.161
更新日期:2017-04-06 00:00:00
abstract::Melanoma is a kind of tumor that originates from melanocytes and is characterized by chemoresistance and distant metastasis. Although the complete pathogenesis of melanoma remains unclear, increasing evidence suggests that circular RNAs (circRNAs) may be involved. In the present study, we identified a circular RNA, ci...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-2444-x
更新日期:2020-04-23 00:00:00
abstract::SHP2 participates in multiple signaling events by mediating T-cell development and function, and regulates cytokine-dependent granulopoiesis. To explore whether and how SHP2 can regulate bone-marrow eosinophil differentiation, we investigate the contribution of SHP2 in the bone-marrow eosinophil development in allergi...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2016.74
更新日期:2016-04-07 00:00:00
abstract::Hyperoxia treatment has been known to induce neuronal and glial death in the developing central nervous system. Retinopathy of prematurity (ROP) is a devastating disease in premature infants and a major cause of childhood vision impairment. Studies indicate that, in addition to vascular injury, retinal neurons are als...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.23
更新日期:2014-02-20 00:00:00
abstract::Esophageal squamous cell carcinoma (ESCC) is one of the deadliest cancers, and it requires novel treatment approaches and effective drugs. In the present study, we found that treatment with plumbagin, a natural compound, reduced proliferation and survival of the KYSE150 and KYSE450 ESCC cell lines in a dose-dependent ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-017-0068-6
更新日期:2018-01-16 00:00:00
abstract::We reported previously that adult (HRAS-/-; NRAS-/-) double knockout (DKO) mice showed no obvious external phenotype although lower-than-expected numbers of weaned DKO animals were consistently tallied after crossing NRAS-KO and HRAS-KO mice kept on mixed genetic backgrounds. Using mouse strains kept on pure C57Bl/6 b...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-2075-2
更新日期:2019-11-04 00:00:00
abstract::Pentose phosphate pathway (PPP) is a major glucose metabolism pathway, which has a fundamental role in cancer growth and metastasis. Even though PPP blockade has been pointed out as a very promising strategy against cancer, effective anti-PPP agents are not still available in the clinical setting. Here we demonstrate ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-0635-5
更新日期:2018-05-01 00:00:00
abstract::We investigated the mechanism underlying the effect of a combination treatment of 125I radioactive seed implantation and lobaplatin (LBP) in hepatocellular carcinoma. The effects of administration of HCC cells and subcutaneous tumor model of mice with different doses of 125I or a sensitizing concentration of LBP alone...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-1918-1
更新日期:2019-10-03 00:00:00
abstract::Senescence is an antiproliferative mechanism that can suppress tumor development and can be induced by oncogenes such as genes of the Ras family. Although studies have implicated bioactive sphingolipids (SL) in senescence, the specific mechanisms remain unclear. Here, using MCF10A mammary epithelial cells, we demonstr...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-03281-4
更新日期:2021-01-04 00:00:00
abstract::Long noncoding RNAs (lncRNAs) have been reported to be involved in a variety of human diseases, including cancers. However, their mechanisms have not yet been fully elucidated. We investigated lncRNA changes that may be associated with pancreatic cancer (PC) by analyzing published microarray data, and identified AGAP2...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-1384-9
更新日期:2019-02-27 00:00:00
abstract::While TRAIL is a promising anticancer agent due to its ability to selectively induce apoptosis in neoplastic cells, many tumors, including pancreatic ductal adenocarcinoma (PDA), display intrinsic resistance, highlighting the need for TRAIL-sensitizing agents. Here we report that TRAIL-induced apoptosis in PDA cell li...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.102
更新日期:2014-03-27 00:00:00
abstract::Lung cancer is the leading cause of cancer-related mortality worldwide. Recently, accumulating data indicate that long noncoding RNAs (LncRNAs) function as novel crucial regulators of diverse biological processes, including proliferation and metastasis, in tumorigenesis. Lnc NONHSAT081507.1 (LINC81507) is associated w...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-1740-9
更新日期:2019-07-11 00:00:00
abstract::The tumor suppressor p53 has critical roles in regulating lipid metabolism, but whether and how p53 regulates cardiolipin (CL) de novo biosynthesis is unknown. Here, we report that p53 physically interacts with histone deacetylase SIRT6 in vitro and in vivo, and this interaction increases following palmitic acid (PA) ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-0984-0
更新日期:2018-09-20 00:00:00
abstract::The subversion of the normal function exerted by the cellular prion protein (PrP(C)) in neurons by pathogenic prions is assumed to have a central role in the pathogenesis of transmissible spongiform encephalopathies. Using two murine models of prion infection, the 1C11 neuronal cell line and neurospheres, we document ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2012.195
更新日期:2013-01-10 00:00:00
abstract::Mediators produced by the airway epithelium control the activation, recruitment, and survival of pulmonary dendritic cells (DC) that present antigen to CD4(+) T cells during the genesis and exacerbation of allergic asthma. The epithelial-derived acute phase protein, serum amyloid A (SAA), induces DC maturation and TH1...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2013.327
更新日期:2013-09-05 00:00:00
abstract::Protein-coding genes account for only ~2% of the human genome, whereas the vast majority of transcripts are non-coding RNAs (ncRNAs) including long ncRNAs (lncRNAs). A growing volume of literature has proposed that lncRNAs are important factors in cancer. Colon cancer-associated transcript-1 (CCAT1), an lncRNA, which ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.541
更新日期:2015-01-08 00:00:00
abstract::Necroptosis is an inflammatory form of programmed cell death requiring receptor-interacting protein kinase 1, 3 (RIPK1, RIPK3) and mixed lineage kinase domain-like protein (MLKL). The kinase of RIPK3 phosphorylates MLKL causing MLKL to form a pore-like structure, allowing intracellular contents to release and cell dea...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2017.20
更新日期:2017-02-02 00:00:00
abstract::We have recently found that primary rat embryonic fibroblasts (REFs) could be immortalized by overexpression of the human mitochondrial ribosomal protein MRPS18-2 (S18-2). A derived cell line, designated 18IM, expressed the embryonic stem cell markers SSEA-1 and Sox2. Upon inoculation into severe combined immunodefici...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2011.138
更新日期:2012-01-19 00:00:00
abstract::The positive results of the apatinib phase III trial have cast new light on treatment for patients with advanced gastric cancer (GC). However, in terms of safety, apatinib toxicities may lead to a dose modification or treatment interruption. Therefore, proper intervention is urgently needed to help patients benefit fr...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-2352-0
更新日期:2020-03-05 00:00:00
abstract::Many cancer therapies are being developed for the induction of durable anti-tumor immunity, especially for malignant tumors. The activation of antigen-presenting cells (APCs), including macrophages and dendritic cells (DCs), can bridge innate and adaptive immune responses against tumors. However, APCs have an immunosu...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-1459-7
更新日期:2019-03-04 00:00:00
abstract::Hereditary pancreatitis (HP) is an autosomal dominant disease that displays the features of both acute and chronic pancreatitis. Mutations in human cationic trypsinogen (PRSS1) are associated with HP and have provided some insight into the pathogenesis of pancreatitis, but mechanisms responsible for the initiation of ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.120
更新日期:2014-04-10 00:00:00
abstract::The WWOX tumor suppressor is a WW domain-containing protein. Its function in the cell has been shown to be mediated, in part, by interacting with its partners through its first WW (WW1) domain. Here, we demonstrated that WWOX via WW1 domain interacts with p53 homolog, ΔNp63α. This protein-protein interaction stabilize...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2013.6
更新日期:2013-01-31 00:00:00
abstract::Aggregatibacter (Actinobacillus) actinomycetemcomitans is a facultative anaerobic gram-negative bacterium associated with severe forms of periodontitis. A leukotoxin, which belongs to the repeats-in-toxin family, is believed to be one of its virulence factors and to have an important role in the bacterium's pathogenic...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2011.6
更新日期:2011-03-10 00:00:00
abstract::The Wilms tumor 1 (WT1)-associated protein (WTAP) is upregulated in many tumors, including, acute myeloid leukemia (AML), where it plays an oncogenic role by interacting with different proteins involved in RNA processing and cell proliferation. In addition, WTAP is also a regulator of the nuclear complex required for ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-0843-z
更新日期:2018-07-23 00:00:00
abstract::Recent studies have documented the diverse role of host immunity in infection by the protozoan parasite, Toxoplasma gondii. However, the contribution of the β-catenin pathway in this process has not been explored. Here, we show that AKT-mediated phosphorylated β-catenin supports T. gondii multiplication which is arres...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-1420-9
更新日期:2019-02-15 00:00:00
abstract::Long noncoding RNAs (lncRNAs) have been identified as potential prognostic tools and therapeutic biomarkers for a variety of human cancers. However, the functional roles and underlying mechanisms of key lncRNAs affecting laryngeal squamous cell carcinomas (LSCCs) are largely unknown. Here, we adopted a novel subpathwa...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-2581-2
更新日期:2020-06-18 00:00:00
abstract::Spermatogonial stem cells (SSCs) are unique male germline stem cells that support spermatogenesis and male fertility. Long non-coding RNAs (lncRNA) have been identified as key regulators of stem cell fate; however, their role in SSCs has not been explored. Here, we report that a novel spermatogonia-specific lncRNA (ln...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2016.24
更新日期:2016-03-10 00:00:00
abstract::α-synuclein (α-syn) accumulation and aggregation is a common pathological factor found in synucleinopathies, a group of neurodegenerative disorders that includes Parkinson´s disease (PD). It has been proposed that lipid dyshomeostasis is responsible for the occurrence of PD-related processes, however, the precise role...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-03254-7
更新日期:2021-01-07 00:00:00