Abstract:
:Puerarin has attracted increasing attention because of its beneficial effects on anti‑osteoporosis, but the molecular mechanisms underlying its actions on osteoblasts are not fully understood. The current study aimed to investigate the effect of puerarin on the cell viability and differentiation of mouse MC3T3‑E1 osteoblast‑like cells in vitro and its underlying mechanisms. The results indicated that 0.01, 0.1 and 1 mg/ml puerarin significantly promoted the viability of osteoblasts, enhanced alkaline phosphatase (ALP) activity and increased the expression of transforming growth factor‑β1, Smad2, Smad3 and Runt‑related transcription factor (Runx)2. Micro (mi)RNA target prediction programs predicted that miR‑204 may directly target Runx2. Following treatment with 0.1 mg/ml puerarin for 48 h, the expression level of miR‑204 was downregulated. Besides, miR‑204 dramatically repressed the luciferase activity of wildtype Runx2 3'‑UTR transfected cells, but not that of the mutant ones. Overexpression of miR‑204 in osteoblasts significantly decreased the protein expression of Runx2, while inhibition of miR‑204 enhanced Runx2's expression. In addition, overexpression of miR‑204 inhibited the cell viability and ALP activity of osteoblasts, while inhibition of miR‑204 had the opposite effect. The results suggested that puerarin may promote MC3T3‑E1 osteoblast‑like cell viability and differentiation, which may be related to the downregulation of miR‑204 and the following activation of Runx2.
journal_name
Mol Med Repjournal_title
Molecular medicine reportsauthors
Zhan XQ,Zeng XW,Zhang YY,Feng Q,Zhao FM,Jiang ZQ,Sun Cdoi
10.3892/mmr.2017.7386subject
Has Abstractpub_date
2017-11-01 00:00:00pages
6262-6268issue
5eissn
1791-2997issn
1791-3004journal_volume
16pub_type
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