The Golgin protein Coy1 functions in intra-Golgi retrograde transport and interacts with the COG complex and Golgi SNAREs.

abstract：:In pancreatic β-cells, insulin granule membranes are enriched in cholesterol and are both recycled and newly generated. Cholesterol's role in supporting granule membrane formation and function is poorly understood. ATP binding cassette transporters ABCG1 and ABCA1 regulate intracellular cholesterol and are important f...

journal_title：Molecular biology of the cell

authors： Hussain SS,Harris MT,Kreutzberger AJB,Inouye CM,Doyle CA,Castle AM,Arvan P,Castle JD

更新日期：2018-05-15 00:00:00

abstract：:Ubiquitin-mediated proteolysis plays a key role in many pathways inside the cell and is particularly important in regulating cell cycle transitions. SCF (Skp1/Cul1/F-box protein) complexes are modular ubiquitin ligases whose specificity is determined by a substrate-binding F-box protein. Dia2 is a Saccharomyces cerevi...

journal_title：Molecular biology of the cell

authors： Koepp DM,Kile AC,Swaminathan S,Rodriguez-Rivera V

更新日期：2006-04-01 00:00:00

abstract：:The formin mDia2 mediates the formation of lamellipodia and filopodia during cell locomotion. The subcellular localization of activated mDia2 depends on interactions with actin filaments and the plasma membrane. We investigated the poorly understood mechanism of plasma membrane targeting of mDia2 and found that the en...

journal_title：Molecular biology of the cell

authors： Gorelik R,Yang C,Kameswaran V,Dominguez R,Svitkina T

更新日期：2011-01-15 00:00:00

abstract：:Many integrin receptors localize to focal contact sites upon binding their ligand. However, unoccupied integrin receptors do not localize to focal contact sites. Because the integrin beta 1 cytoplasmic domain appears to have a focal contact localization signal, there must be a mechanism by which this domain is kept in...

journal_title：Molecular biology of the cell

authors： Briesewitz R,Kern A,Marcantonio EE

更新日期：1993-06-01 00:00:00

abstract：:Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for epithelial polarity. A fraction of Stx3 is localized to late endosomes/lysosomes, although how it traffics there and its function in these organelles is unknown. Here we report that Stx3 underg...

journal_title：Molecular biology of the cell

authors： Giovannone AJ,Reales E,Bhattaram P,Fraile-Ramos A,Weimbs T

更新日期：2017-10-15 00:00:00

abstract：:To determine how the human cdc25 gene product acts to regulate p34cdc2 at the G2 to M transition, we have overproduced the full-length protein (cdc25Hs) as well as several deletion mutants in bacteria as glutathione-S-transferase fusion proteins. The wild-type cdc25Hs gene product was synthesized as an 80-kDa fusion p...

journal_title：Molecular biology of the cell

authors： Lee MS,Ogg S,Xu M,Parker LL,Donoghue DJ,Maller JL,Piwnica-Worms H

更新日期：1992-01-01 00:00:00

abstract：:Notch and neurotrophins control neuronal shape, but it is not known whether their signaling pathways intersect. Here we report results from hippocampal neuronal cultures that are in support of this possibility. We found that low cell density or blockade of Notch signaling by a soluble Delta-Fc ligand decreased the mRN...

journal_title：Molecular biology of the cell

authors： Salama-Cohen P,Arévalo MA,Meier J,Grantyn R,Rodríguez-Tébar A

更新日期：2005-01-01 00:00:00

abstract：:MCAK belongs to the Kinesin-13 family, whose members depolymerize microtubules rather than translocate along them. We defined the minimal functional unit of MCAK as the catalytic domain plus the class specific neck (MD-MCAK), which is consistent with previous reports. We used steady-state ATPase kinetics, microtubule ...

journal_title：Molecular biology of the cell

authors： Hertzer KM,Ems-McClung SC,Kline-Smith SL,Lipkin TG,Gilbert SP,Walczak CE

更新日期：2006-02-01 00:00:00

abstract：:The cytoskeleton plays an important role in neuronal morphogenesis. We have identified and characterized a novel actin-binding protein, termed Mayven, predominantly expressed in brain. Mayven contains a BTB (broad complex, tramtrack, bric-a-brac)/POZ (poxvirus, zinc finger) domain-like structure in the predicted N ter...

journal_title：Molecular biology of the cell

authors： Soltysik-Espanola M,Rogers RA,Jiang S,Kim TA,Gaedigk R,White RA,Avraham H,Avraham S

更新日期：1999-07-01 00:00:00

abstract：:The skin's epidermis is a multilayered epithelial tissue and the first line of defense against mechanical stress. Its barrier function depends on an integrated assembly and reorganization of cell-matrix and cell-cell junctions in the basal layer and on different intercellular junctions in suprabasal layers. However, h...

journal_title：Molecular biology of the cell

authors： Noethel B,Ramms L,Dreissen G,Hoffmann M,Springer R,Rübsam M,Ziegler WH,Niessen CM,Merkel R,Hoffmann B

更新日期：2018-09-15 00:00:00

abstract：:DSL1 was identified through its genetic interaction with SLY1, which encodes a t-SNARE-interacting protein that functions in endoplasmic reticulum (ER)-to-Golgi traffic. Conditional dsl1 mutants exhibit a block in ER-to-Golgi traffic at the restrictive temperature. Here, we show that dsl1 mutants are defective for ret...

journal_title：Molecular biology of the cell

authors： Reilly BA,Kraynack BA,VanRheenen SM,Waters MG

更新日期：2001-12-01 00:00:00

abstract：:Calcium (Ca(2+)) and calmodulin (CaM) are required for progression of mammalian cells from quiescence into S phase. In multiple cell types, cyclosporin A causes a G(1) cell cycle arrest, implicating the serine/threonine phosphatase calcineurin as one Ca(2+)/CaM-dependent enzyme required for G(1) transit. Here, we show...

journal_title：Molecular biology of the cell

authors： Kahl CR,Means AR

更新日期：2004-04-01 00:00:00

abstract：:The resistance of Candida albicans to many stresses is dependent on the stress-activated protein kinase (SAPK) Hog1. Hence we have explored the role of Hog1 in the regulation of transcriptional responses to stress. DNA microarrays were used to characterize the global transcriptional responses of HOG1 and hog1 cells to...

journal_title：Molecular biology of the cell

authors： Enjalbert B,Smith DA,Cornell MJ,Alam I,Nicholls S,Brown AJ,Quinn J

更新日期：2006-02-01 00:00:00

abstract：:In most eukaryotes, genes encoding ribosomal RNAs (rDNA) are clustered in long tandem head-to-tail repeats. Studies of Saccharomyces cerevisiae have indicated that rDNA copy number is maintained through recombination events associated with site-specific blockage of replication forks (RFs). Here, we describe two Schizo...

journal_title：Molecular biology of the cell

authors： Coulon S,Gaillard PHL,Chahwan C,McDonald WH,Yates JR 3rd,Russell P

更新日期：2004-01-01 00:00:00

abstract：:Candida albicans, the major human fungal pathogen, undergoes a reversible morphological transition from blastospores (round budding cells) to filaments (elongated cells attached end-to-end). This transition, which is induced upon exposure of C. albicans cells to a number of host conditions, including serum and body te...

journal_title：Molecular biology of the cell

authors： Kadosh D,Johnson AD

更新日期：2005-06-01 00:00:00

abstract：:In Saccharomyces cerevisiae, many osmotically inducible genes are regulated by the Sko1p-Ssn6p-Tup1p complex. On osmotic shock, the MAP kinase Hog1p associates with this complex, phosphorylates Sko1p, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. We have found that phospholip...

journal_title：Molecular biology of the cell

authors： Guha N,Desai P,Vancura A

更新日期：2007-07-01 00:00:00

abstract：:SPOP, speckle-type POZ protein is a substrate adaptor protein of the Cullin-3/RING ubiquitin E3 complex. The spop gene is the most commonly point mutated in human primary prostate cancers, but the pathological contribution of the SPOP mutations remains unclear. In this study, we investigated several known factors that...

journal_title：Molecular biology of the cell

authors： Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

更新日期：2020-03-15 00:00:00

abstract：:The exocyst is an evolutionarily conserved octameric protein complex that tethers post-Golgi secretory vesicles at the plasma membrane for exocytosis. To elucidate the mechanism of vesicle tethering, it is important to understand how the exocyst physically associates with the plasma membrane (PM). In this study, we re...

journal_title：Molecular biology of the cell

authors： Liu J,Zuo X,Yue P,Guo W

更新日期：2007-11-01 00:00:00

abstract：:We have studied telomere length in Schizosaccharomyces pombe strains carrying mutations affecting cell cycle checkpoints, DNA repair, and regulation of the Cdc2 protein kinase. Telomere shortening was found in rad1, rad3, rad17, and rad26 mutants. Telomere lengths in previously characterized rad1 mutants paralleled th...

journal_title：Molecular biology of the cell

authors： Dahlen M,Olsson T,Kanter-Smoler G,Ramne A,Sunnerhagen P

更新日期：1998-03-01 00:00:00

abstract：:Trafficking through the Golgi apparatus requires members of the Arf family of GTPases, whose activation is regulated by guanine nucleotide exchange factors (GEFs). Once activated, Arf-GTP recruits effectors such as coat complexes and lipid-modifying enzymes to specific membrane sites, creating a domain competent for c...

journal_title：Molecular biology of the cell

authors： Niu TK,Pfeifer AC,Lippincott-Schwartz J,Jackson CL

更新日期：2005-03-01 00:00:00

abstract：:CWH41, a gene involved in the assembly of cell wall beta-1,6-glucan, has recently been shown to be the structural gene for Saccharomyces cerevisiae glucosidase I that is responsible for initiating the trimming of terminal alpha-1,2-glucose residue in the N-glycan processing pathway. To distinguish between a direct or ...

journal_title：Molecular biology of the cell

authors： Abeijon C,Chen LY

更新日期：1998-10-01 00:00:00

abstract：:We have shown previously that the Swi5 transcription factor regulates the expression of the SIC1 Cdk inhibitor in late mitosis. This suggests that Swi5 might control other genes with roles in ending mitosis. We identified a gene with a Swi5-binding site in the promoter that encoded a protein with high homology to Pcl2...

journal_title：Molecular biology of the cell

authors： Aerne BL,Johnson AL,Toyn JH,Johnston LH

更新日期：1998-04-01 00:00:00

abstract：:Sec1p/Munc18 (SM) family proteins regulate SNARE complex function in membrane fusion through their interactions with syntaxins. In addition to syntaxins, only a few SM protein interacting proteins are known and typically, their binding modes with SM proteins are poorly characterized. We previously identified Mso1p as ...

journal_title：Molecular biology of the cell

authors： Weber M,Chernov K,Turakainen H,Wohlfahrt G,Pajunen M,Savilahti H,Jäntti J

更新日期：2010-04-15 00:00:00

abstract：:The assembly of metazoan Sm-class small nuclear ribonucleoproteins (snRNPs) is an elaborate, step-wise process that takes place in multiple subcellular compartments. The initial steps, including formation of the core RNP, are mediated by the survival motor neuron (SMN) protein complex. Loss-of-function mutations in hu...

journal_title：Molecular biology of the cell

authors： Shpargel KB,Praveen K,Rajendra TK,Matera AG

更新日期：2009-01-01 00:00:00

abstract：:The yeast Golgi membrane protein Rer1p is required for the retrieval of various endoplasmic reticulum (ER) membrane proteins such as Sec12p and Sec71p to the ER. We demonstrate here that the transmembrane domain (TMD) of Sec71p, a type-III membrane protein, contains an ER localization signal, which is required for phy...

journal_title：Molecular biology of the cell

authors： Sato K,Sato M,Nakano A

更新日期：2003-09-01 00:00:00

abstract：:Important aspects of cell cycle regulation are the checkpoints, which respond to a variety of cellular stresses to inhibit cell cycle progression and act as protective mechanisms to ensure genomic integrity. An increasing number of tumor suppressors are being demonstrated to have roles in checkpoint mechanisms, implyi...

journal_title：Molecular biology of the cell

authors： Qiu L,Burgess A,Fairlie DP,Leonard H,Parsons PG,Gabrielli BG

更新日期：2000-06-01 00:00:00

abstract：:The Src homology (SH2 and SH3) domains of v-Src are required for transformation of Rat-2 cells and for wild-type (morphr) transformation of chicken embryo fibroblasts (CEFs). We report herein that the N-terminal domains of v-Src, when expressed in trans, cannot complement the transformation defect of a deletion mutant...

journal_title：Molecular biology of the cell

authors： Tian M,Martin GS

更新日期：1997-07-01 00:00:00

abstract：:Posttranslationally modified forms of tubulin accumulate in the subset of stabilized microtubules (MTs) in cells but are not themselves involved in generating MT stability. We showed previously that stabilized, detyrosinated (Glu) MTs function to localize vimentin intermediate filaments (IFs) in fibroblasts. To determ...

journal_title：Molecular biology of the cell

authors： Kreitzer G,Liao G,Gundersen GG

更新日期：1999-04-01 00:00:00

abstract：:Severe heat stress causes protein denaturation in various cellular compartments. If Saccharomyces cerevisiae cells grown at 24 degrees C are preconditioned at 37 degrees C, proteins denatured by subsequent exposure to 48-50 degrees C can be renatured when the cells are allowed to recover at 24 degrees C. Conformationa...

journal_title：Molecular biology of the cell

authors： Hänninen AL,Simola M,Saris N,Makarow M

更新日期：1999-11-01 00:00:00

abstract：:Tumor necrosis factor α (TNF-α) receptor-associated factor 2 (TRAF2) regulates activation of the c-Jun N-terminal kinase (JNK)/c-Jun and the inhibitor of κB kinase (IKK)/nuclear factor κB (NF-κB) signaling cascades in response to TNF-α stimulation. Gene knockout studies have revealed that TRAF2 inhibits TNF-α-induced ...

journal_title：Molecular biology of the cell

authors： Zhang L,Blackwell K,Altaeva A,Shi Z,Habelhah H

更新日期：2011-01-01 00:00:00