Abstract:
:Agrobacterium tumefaciens mediated T-DNA integration is a common tool for plant genome manipulation. However, there is controversy regarding whether T-DNA integration is biased towards genes or randomly distributed throughout the genome. In order to address this question, we performed high-throughput mapping of T-DNA-genome junctions obtained in the absence of selection at several time points after infection. T-DNA-genome junctions were detected as early as 6 hours post-infection. T-DNA distribution was apparently uniform throughout the chromosomes, yet local biases toward AT-rich motifs and T-DNA border sequence micro-homology were detected. Analysis of the epigenetic landscape of previously isolated sites of T-DNA integration in Kanamycin-selected transgenic plants showed an association with extremely low methylation and nucleosome occupancy. Conversely, non-selected junctions from this study showed no correlation with methylation and had chromatin marks, such as high nucleosome occupancy and high H3K27me3, that correspond to three-dimensional-interacting heterochromatin islands embedded within euchromatin. Such structures may play a role in capturing and silencing invading T-DNA.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Shilo S,Tripathi P,Melamed-Bessudo C,Tzfadia O,Muth TR,Levy AAdoi
10.1371/journal.pgen.1006875subject
Has Abstractpub_date
2017-07-24 00:00:00pages
e1006875issue
7eissn
1553-7390issn
1553-7404pii
PGENETICS-D-17-00273journal_volume
13pub_type
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