Abstract:
:Relapse and metastasis of nasopharyngeal carcinoma (NPC) are presumably attributed to cancer stem cells (CSCs). In recent years, chimeric antigen receptor (CAR)-modified immune effector cells have been shown to have impressive antitumour efficacy. In this study, we aimed to identify appropriate tumour-associated antigens predominantly expressed on NPC stem cells (NPCSCs) and determine their suitability for CAR-engineered cytokine-induced killer (CIK) cell therapy against NPC. By investigating the expression patterns of potential target antigens (ROR1, 5T4 and CAIX) in NPC, we found that the oncofetal antigen 5T4 was predominately expressed in NPC cell lines and tissues but absent in non-cancerous nasopharyngeal tissues. Moreover, significantly enhanced expression of 5T4 in NPC spheroids revealed its relationship with putative NPCSCs. Hence, we designed a CAR construct (5T4-28Z) specific for 5T4 and generated CAR-transduced CIK cells. Our results showed that the artificial CAR was efficiently expressed on the surface of CIK cells and that no native phenotypes were altered by the gene transduction. Functional assays revealed that 5T4-28Z-CIK cells possessed both CAR-mediated and CAR-independent anti-NPC activity and were capable of efficiently attacking NPC cells, especially NPCSC-like cells in vitro, suggesting that they might serve as an attractive tool for developing efficient therapies against NPC.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Guo X,Zheng H,Luo W,Zhang Q,Liu J,Yao Kdoi
10.1038/s41598-017-04756-9subject
Has Abstractpub_date
2017-07-07 00:00:00pages
4859issue
1issn
2045-2322pii
10.1038/s41598-017-04756-9journal_volume
7pub_type
杂志文章abstract::We aimed to investigate the diagnostic accuracy of contrast-enhanced ultrasound (CEUS) in evaluating blunt abdominal trauma for patients presenting to the emergency department. Electronic search of Scopus and Pubmed was performed from inception to September 2016. Human studies investigating the diagnostic accuracy of ...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-017-04779-2
更新日期:2017-06-30 00:00:00
abstract::LytR-cpsA-Psr (LCP) domain containing proteins fulfil important functions in bacterial cell wall synthesis. In Mycobacterium tuberculosis complex (Mtbc) strains, the causative agents of tuberculosis (TB), the genes Rv3484 and Rv3267 encode for LCP proteins which are putatively involved in arabinogalactan transfer to p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22012-6
更新日期:2018-03-02 00:00:00
abstract::Ambient seismic noise is characterized by randomness incurred by the random position and strength of the noise sources as well as the heterogeneous properties of the medium through which it propagates. Here we use ambient noise data recorded prior to the 1996 Gjálp eruption in Iceland in order to show that a reduction...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37733
更新日期:2016-11-24 00:00:00
abstract::Parasites comprise a huge part of the biodiversity on earth. However, on a local scale, not much is known about their diversity and community structure. Here, we assess the diversity of larval trematode communities in an interconnected freshwater system of the River Ruhr in Germany and analyse how the parasites are sp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59548-5
更新日期:2020-02-14 00:00:00
abstract::During Antarctic isolation personnel are exposed to extreme photoperiods. A frequent observation is a sleep onset phase delay during winter. It is not known if, as a result, daytime sleeping in the form of naps increases. We sought to assess sleep patterns - with focus on daytime sleeping - and alertness in a Latin Am...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46900-7
更新日期:2019-07-26 00:00:00
abstract::A comprehensive understanding of the charge/discharge behaviour of high-capacity anode active materials, e.g., Si and Li, is essential for the design and development of next-generation high-performance Li-based batteries. Here, we demonstrate the in situ scanning electron microscopy (in situ SEM) of Si anodes in a con...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36153
更新日期:2016-10-26 00:00:00
abstract::Maturity stage in harvesting time greatly affects seed vigour. This work aimed to scientific harvesting time of hybrid rice for being high vigour with high & stable seed yield. Field experiments of different harvesting time were conducted in 2013-2014, and germination percentage (GP), vigour index (VI), seed moisture ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29021-5
更新日期:2018-07-23 00:00:00
abstract::We present here a combined theoretical and experimental investigation on effective excitation of microcantilever by using photoacoustic waves. The photoacoustic waves arose from a vibrating Al foil induced by an intensity-modulated laser. We demonstrate that, superior to photothermal excitation, this new configuration...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19935
更新日期:2016-01-27 00:00:00
abstract::Low Haemoglobin concentration (Hb) among women of reproductive age is a severe public health problem in sub-Saharan Africa. This study investigated the effects of putative socio-demographic factors on maternal Hb at different points of the conditional distribution of Hb concentration. We utilised quantile regression t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78617-3
更新日期:2020-12-07 00:00:00
abstract::Glaulactams A-C (1-3), which possess a novel skeleton, as well as the known compound daphmanidin B (4), were isolated from the leaves of Daphniphyllum glaucescens and separated using ion-exchange chromatography aided by NMR fingerprinting. Their structures, including their absolute configurations, were elucidated by s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33748-6
更新日期:2018-10-18 00:00:00
abstract::The growing interest in engineered tumor models prompted us to devise a method for the non-invasive assessment of such models. Here, we report on bioluminescence imaging (BLI) for the assessment of engineered tumor models in the fertilized chicken egg, i.e, chick chorioallantoic membrane (CAM) assay. One prostate canc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04572-1
更新日期:2017-07-10 00:00:00
abstract::Persistent human papillomavirus (HPV) infection is the vital factor driving cervical carcinogenesis; however, other features of the local cervicovaginal microenvironment (CVM) may play a critical role in development of precancerous cervical dysplasia and progression to invasive cervical carcinoma (ICC). Here we invest...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43849-5
更新日期:2019-05-14 00:00:00
abstract::The synthesis and subsequent genomic integration of DNA that is complementary to the genomes of non-retroviral RNA viruses are rarely observed. However, upon infection of various human cell lines and primary fibroblasts with the vesicular stomatitis virus (VSV), we detected DNA complementary to the VSV RNA. The VSV DN...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05074
更新日期:2014-05-30 00:00:00
abstract::The precision and efficiency of the registration of megavolt-level electronic portal imaging devices (EPID) images with the naked eye in the orthogonal window are reduced. This study aims to develop a new registration algorithm with enhanced accuracy and efficiency. Ten setup errors with different translation and rota...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67331-9
更新日期:2020-06-24 00:00:00
abstract::Separation of enantiomers by flows is a promising chiral resolution method using cost-effective microfluidics. Notwithstanding a number of experimental and numerical studies, a fundamental understanding still remains elusive, and an important question as to whether it is possible to specify common physical properties ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35144
更新日期:2016-10-14 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-69834-x
更新日期:2020-07-29 00:00:00
abstract::Platelet-derived growth factor receptor beta (PDGFRβ) affects in numerous human cancers and has been recognized as a promising molecular target for cancer therapies. The overexpression of PDGFRβ could be a biomarker for cancer diagnosis. Radiolabeled ligands having high affinity for the molecular target could be usefu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28529-0
更新日期:2018-07-10 00:00:00
abstract::Bi-phasic or diauxic growth is often observed when microbes are grown in a chemically defined medium containing two sugars (for example glucose and lactose). Typically, the two growth stages are separated by an often lengthy phase of arrested growth, the so-called lag-phase. Diauxic growth is usually interpreted as an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25191
更新日期:2016-04-29 00:00:00
abstract::Silicon nanocrystals offer huge advantages compared to other semi-conductor quantum dots as they are made from an abundant, non-toxic material and are compatible with silicon devices. Besides, among a wealth of extraordinary properties ranging from catalysis to nanomedicine, metal nanoparticles are known to increase t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02672
更新日期:2013-01-01 00:00:00
abstract::Although rare in the U.S., outbreaks due to Neisseria meningitidis do occur. Rapid, early outbreak detection is important for timely public health response. In this study, we characterized U.S. meningococcal isolates (N = 201) from 15 epidemiologically defined outbreaks (2009-2015) along with temporally and geographic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33622-5
更新日期:2018-10-25 00:00:00
abstract::Despite increasing work detailing the presence of foraging specializations across a range of taxa, limited attention so far has been given to the role of spatiotemporal variation in food predictability in shaping individual resource selection. Here, we studied the exploitation of human-provided carrion resources diffe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33564-y
更新日期:2018-10-11 00:00:00
abstract::Nowadays, one of the main challenges is moving towards an eco-sustainable agriculture, able to preserve the food production through a reduced use of pesticides. Current global food sustenance by intensive agriculture is mainly based on economic crop monocultures and drastically reduces the biodiversity, increasing the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76247-3
更新日期:2020-11-05 00:00:00
abstract::A generation of reactive oxygen species (ROS) from TiO2 under solar light has been long sought since the ROS can disinfect organic pollutants. We found that newly developed crystalline/amorphous reduced TiO2 (rTiO2) that has low energy bandgap can effectively generate ROS under solar light and successfully remove a bl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25212
更新日期:2016-04-28 00:00:00
abstract::High performance of organic tandem solar cell is largely dependent on transparent and conductive intermediate layer (IML). The current work reports the design and fabrication of an IML using a simple solution process. The efficiency of a homo-tandem device with poly(3-hexylthiophene):phenyl-C61-butyric acid methyl est...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30327
更新日期:2016-07-25 00:00:00
abstract::Emergence of new molecules acting directly on the hepatitic C virus (HCV) has improved treatment outcomes. However, there is a risk of selecting viral escape mutants, so a new combination is needed using different inhibitors that target different steps of the HCV infectious cycle. Novel single tablet formulations were...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49973-6
更新日期:2019-09-19 00:00:00
abstract::Protoporphyrin IX (PpIX) induced by 5-aminolevulinic acid (5-ALA) is increasingly used as a fluorescent marker for fluorescence-guided resection of malignant gliomas. Understanding how the properties of the excitation light source and PpIX fluorescence interact with the surgical microscope is critical for effective us...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30247-6
更新日期:2018-08-22 00:00:00
abstract::We present an optimized non-thermal atmospheric plasma (NTAP)-based reaction-discharge system that was applied for a continuous-flow treatment of apple juice (AJ). To optimize this system for a high-throughput production of AJ with ameliorated properties, the effect of several parameters was studied using design of ex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78131-6
更新日期:2020-12-03 00:00:00
abstract::The G protein-coupled pituitary adenylate cyclase-activating polypeptide receptor (PAC1R) is a potential therapeutic target for endocrine, metabolic and stress-related disorders. However, many questions regarding the protein structure and dynamics of PAC1R remain largely unanswered. Using microsecond-long simulations,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05815-x
更新日期:2017-07-14 00:00:00
abstract::The objective of this study was to unveil the potential mechanism of focused ultrasound (FUS)-enhanced intranasal (IN) brain drug delivery and assess its feasibility in the delivery of therapeutic molecules. Delivery outcomes of fluorescently-labeled dextrans to mouse brains by IN administration either before or after...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28599
更新日期:2016-06-27 00:00:00
abstract::In response to infestation by herbivores, rice plants rapidly biosynthesize defense compounds by activating a series of defense-related pathways. However, which defensive compounds in rice are effective against herbivores remains largely unknown. We found that the infestation of white-backed planthopper (WBPH) Sogatel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62752-y
更新日期:2020-04-01 00:00:00