Massively parallel nanowell-based single-cell gene expression profiling.

abstract：BACKGROUND:Horses have been strongly selected for speed, strength, and endurance-exercise traits since the onset of domestication. As a result, highly specialized horse breeds have developed with many modern horse breeds often representing closed populations with high phenotypic and genetic uniformity. However, a great...

journal_title：BMC genomics

authors： Velie BD,Lillie M,Fegraeus KJ,Rosengren MK,Solé M,Wiklund M,Ihler CF,Strand E,Lindgren G

更新日期：2019-02-04 00:00:00

abstract：BACKGROUND:Many plant genes have been identified through whole genome and deep transcriptome sequencing and other methods; yet our knowledge on the function of many of these genes remains limited. The integration and analysis of large gene-expression datasets gives researchers the ability to formalize hypotheses concer...

journal_title：BMC genomics

authors： Wilson TJ,Lai L,Ban Y,Ge SX

更新日期：2012-06-13 00:00:00

abstract：BACKGROUND:Although second generation sequencing (2GS) technologies allow re-sequencing of previously gold-standard-sequenced genomes, whole genome shotgun sequencing and de novo assembly of large and complex eukaryotic genomes is still difficult. Availability of a genome-wide physical map is therefore still a prerequi...

journal_title：BMC genomics

authors： Schulte D,Ariyadasa R,Shi B,Fleury D,Saski C,Atkins M,deJong P,Wu CC,Graner A,Langridge P,Stein N

更新日期：2011-05-19 00:00:00

abstract：BACKGROUND:Animals are thought to achieve lignocellulose digestion via symbiotic associations with gut microbes; this view leads to significant focus on bacteria and fungi for lignocellulolytic systems. The presence of biomass conversion systems hardwired into animal genomes has not yet been unequivocally demonstrated....

journal_title：BMC genomics

authors： Chang WH,Lai AG

更新日期：2018-06-20 00:00:00

abstract：BACKGROUND:Calcium-dependent protein kinase (CPK) is one of the main Ca2+ combined protein kinase that play significant roles in plant growth, development and response to multiple stresses. Despite an important member of the stress responsive gene family, little is known about the evolutionary history and expression pa...

journal_title：BMC genomics

authors： Zhang M,Liu Y,He Q,Chai M,Huang Y,Chen F,Wang X,Liu Y,Cai H,Qin Y

更新日期：2020-01-23 00:00:00

abstract：BACKGROUND:Cichlid fishes have evolved remarkably diverse reproductive, social, and feeding behaviors. Cell-to-cell signaling molecules, notably neuropeptides and peptide hormones, are known to regulate these behaviors across vertebrates. This class of signaling molecules derives from prohormone genes that have undergo...

journal_title：BMC genomics

authors： Hu CK,Southey BR,Romanova EV,Maruska KP,Sweedler JV,Fernald RD

更新日期：2016-08-19 00:00:00

abstract：BACKGROUND:Controlling and managing the breeding of bluefin tuna (Thunnus spp.) in captivity is an imperative step towards obtaining a sustainable supply of these fish in aquaculture production systems. Germ cell transplantation (GCT) is an innovative technology for the production of inter-species surrogates, by transp...

journal_title：BMC genomics

authors： Bar I,Cummins S,Elizur A

更新日期：2016-03-10 00:00:00

abstract：BACKGROUND:The measurement of gene expression using microarray technology is a complicated process in which a large number of factors can be varied. Due to the lack of standard calibration samples such as are used in traditional chemical analysis it may be a problem to evaluate whether changes done to the microarray pr...

journal_title：BMC genomics

authors： Bruland T,Anderssen E,Doseth B,Bergum H,Beisvag V,Laegreid A

更新日期：2007-10-18 00:00:00

abstract：BACKGROUND:Meiotic recombination is a fundamental evolutionary process. It not only generates diversity, but influences the efficacy of natural selection and genome evolution. There can be significant heterogeneity in recombination rates within and between species, however this variation is not well understood outside ...

journal_title：BMC genomics

authors： Gion JM,Hudson CJ,Lesur I,Vaillancourt RE,Potts BM,Freeman JS

更新日期：2016-08-09 00:00:00

abstract：BACKGROUND:Atlantic salmon production in Tasmania (Southern Australia) occurs near the upper limits of the species thermal tolerance. Summer water temperatures can average over 19 °C over several weeks and have negative effects on performance and health. Liver tissue exerts important metabolic functions in thermal adap...

journal_title：BMC genomics

authors： Nuez-Ortín WG,Carter CG,Nichols PD,Cooke IR,Wilson R

更新日期：2018-02-12 00:00:00

abstract：BACKGROUND:By assaying hundreds of thousands of single nucleotide polymorphisms, genome wide association studies (GWAS) allow for a powerful, unbiased review of the entire genome to localize common genetic variants that influence health and disease. Although it is widely recognized that some correction for multiple tes...

journal_title：BMC genomics

authors： Duggal P,Gillanders EM,Holmes TN,Bailey-Wilson JE

更新日期：2008-10-31 00:00:00

abstract：BACKGROUND:The identification and quantification of proteins using label-free Liquid Chromatography/Mass Spectrometry (LC/MS) play crucial roles in biological and biomedical research. Increasing evidence has shown that biomarkers are often low abundance proteins. However, LC/MS systems are subject to considerable noise...

journal_title：BMC genomics

authors： Zhang J,Haskins W

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:Many biological systems respond to the presence or absence of gravity. Since experiments performed in space are expensive and can only be undertaken infrequently, Earth-based simulation techniques are used to investigate the biological response to weightlessness. A high gradient magnetic field can be used to...

journal_title：BMC genomics

authors： Herranz R,Larkin OJ,Dijkstra CE,Hill RJ,Anthony P,Davey MR,Eaves L,van Loon JJ,Medina FJ,Marco R

更新日期：2012-02-01 00:00:00

abstract：BACKGROUND:Incilaria (= Meghimatium) fruhstorferi is an air-breathing land slug found in restricted habitats of Japan, Taiwan and selected provinces of South Korea (Jeju, Chuncheon, Busan, and Deokjeokdo). The species is on a decline due to depletion of forest cover, predation by natural enemies, and collection. To fac...

journal_title：BMC genomics

authors： Patnaik BB,Chung JM,Hwang HJ,Sang MK,Park JE,Min HR,Cho HC,Dewangan N,Baliarsingh S,Kang SW,Park SY,Jo YH,Park HS,Kim WJ,Han YS,Lee JS,Lee YS

更新日期：2019-02-26 00:00:00

abstract：BACKGROUND:The ability to identify regions of the genome inherited with a dominant trait in one or more families has become increasingly valuable with the wide availability of high throughput sequencing technology. While a number of methods exist for mapping of homozygous variants segregating with recessive traits in c...

journal_title：BMC genomics

authors： Levine AP,Connor TM,Oygar DD,Neild GH,Segal AW,Maxwell PH,Gale DP

更新日期：2015-03-10 00:00:00

abstract：BACKGROUND:Calcium ion is tightly regulated in body fluids and for euryhaline fish, which are exposed to rapid changes in environmental [Ca2+], homeostasis is especially challenging. The gill is the main organ of active calcium uptake and therefore plays a crucial role in the maintenance of calcium ion homeostasis. To ...

journal_title：BMC genomics

authors： Pinto PI,Matsumura H,Thorne MA,Power DM,Terauchi R,Reinhardt R,Canário AV

更新日期：2010-08-17 00:00:00

abstract：BACKGROUND:Ubiquitination is an important post-translational modification involved in diverse biological processes. Therefore, genomewide representation of the ubiquitination system for a species is important. DESCRIPTION:SCUD is a web-based database for the ubiquitination system in Saccharomyces cerevisiae (Baker's y...

journal_title：BMC genomics

authors： Lee WC,Lee M,Jung JW,Kim KP,Kim D

更新日期：2008-09-24 00:00:00

abstract：BACKGROUND:In recent years, the number of human infections caused by opportunistic pathogens has increased dramatically. Plant rhizospheres are one of the most typical natural reservoirs for these pathogens but they also represent a great source for beneficial microbes with potential for biotechnological applications. ...

journal_title：BMC genomics

authors： Alavi P,Starcher MR,Thallinger GG,Zachow C,Müller H,Berg G

更新日期：2014-06-18 00:00:00

abstract：BACKGROUND:Ecological studies routinely show genotype-genotype interactions between insects and their parasites. The mechanisms behind these interactions are not clearly understood. Using the bumblebee Bombus terrestris/trypanosome Crithidia bombi model system (two bumblebee colonies by two Crithidia strains), we have ...

journal_title：BMC genomics

authors： Riddell CE,Lobaton Garces JD,Adams S,Barribeau SM,Twell D,Mallon EB

更新日期：2014-11-27 00:00:00

abstract：BACKGROUND:Gene ontology analysis using the microarray database generated in a previous study by this laboratory was used to further evaluate how maternal dietary supplementation with pyridoxine combined with different sources of selenium (Se) affected global gene expression of expanded porcine blastocysts. Data were g...

journal_title：BMC genomics

authors： Dalto DB,Tsoi S,Dyck MK,Matte JJ

更新日期：2018-11-21 00:00:00

abstract：BACKGROUND:The use of RNAseq to resolve the transcriptional organization of an organism was established in recent years and also showed the complexity and dynamics of bacterial transcriptomes. The aim of this study was to comprehensively investigate the transcriptome of the industrially relevant amino acid producer and...

journal_title：BMC genomics

authors： Pfeifer-Sancar K,Mentz A,Rückert C,Kalinowski J

更新日期：2013-12-17 00:00:00

abstract：BACKGROUND:The transcriptome of the fruit pulp of the sweet orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening. RESULTS:The transcriptomes of WT and MT were sampled at...

journal_title：BMC genomics

authors： Yu K,Xu Q,Da X,Guo F,Ding Y,Deng X

更新日期：2012-01-10 00:00:00

abstract：BACKGROUND:Heat shock proteins (Hsps) are molecular chaperones that are involved in many normal cellular processes and stress responses, and heat shock factors (Hsfs) are the transcriptional activators of Hsps. Hsfs and Hsps are widely coordinated in various biological processes. Although the roles of Hsfs and Hsps in ...

journal_title：BMC genomics

authors： Zhang J,Liu B,Li J,Zhang L,Wang Y,Zheng H,Lu M,Chen J

更新日期：2015-03-14 00:00:00

abstract：BACKGROUND:Characterizing large genomic variants is essential to expanding the research and clinical applications of genome sequencing. While multiple data types and methods are available to detect these structural variants (SVs), they remain less characterized than smaller variants because of SV diversity, complexity,...

journal_title：BMC genomics

authors： English AC,Salerno WJ,Hampton OA,Gonzaga-Jauregui C,Ambreth S,Ritter DI,Beck CR,Davis CF,Dahdouli M,Ma S,Carroll A,Veeraraghavan N,Bruestle J,Drees B,Hastie A,Lam ET,White S,Mishra P,Wang M,Han Y,Zhang F,Stankie

更新日期：2015-04-11 00:00:00

abstract：BACKGROUND:Litter size in pigs is a major factor affecting the profitability in the pig industry. The peri-implantation window in pigs is characterized by the coordinated interactions between the maternal uterine endometrium and the rapidly elongating conceptuses and represents a period of time during which a large per...

journal_title：BMC genomics

authors： Gu T,Zhu MJ,Schroyen M,Qu L,Nettleton D,Kuhar D,Lunney JK,Ross JW,Zhao SH,Tuggle CK

更新日期：2014-02-24 00:00:00

abstract：BACKGROUND:Our previous study had proved that nigericin could reduce colorectal cancer cell proliferation in dose- and time-dependent manners by targeting Wnt/β-catenin signaling. To better elucidate its potential anti-cancer mechanism, two pancreatic cancer (PC) cell lines were exposed to increasing concentrations of ...

journal_title：BMC genomics

authors： Xu Z,Shen J,Hua S,Wan D,Chen Q,Han Y,Ren R,Liu F,Du Z,Guo X,Shi J,Zhi Q

更新日期：2019-09-18 00:00:00

abstract：BACKGROUND:The steroid hormone environment in healthy horses seems to have a significant impact on the efficiency of their uterine immune response. The objective of this study was to characterize the changes in gene expression in the equine endometrium in response to the introduction of bacterial pathogens and the infl...

journal_title：BMC genomics

authors： Marth CD,Young ND,Glenton LY,Noden DM,Browning GF,Krekeler N

更新日期：2015-11-14 00:00:00

abstract：BACKGROUND:Single cell transcriptome sequencing has become an increasingly valuable technology for dissecting complex biology at a resolution impossible with bulk sequencing. However, the gap between the technical expertise required to effectively work with the resultant high dimensional data and the biological experti...

journal_title：BMC genomics

authors： Feng D,Whitehurst CE,Shan D,Hill JD,Yue YG

更新日期：2019-08-27 00:00:00

abstract：BACKGROUND:The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA se...

journal_title：BMC genomics

authors： Wang WW,Wang J,Zhang HJ,Wu SG,Qi GH

更新日期：2019-12-12 00:00:00

abstract：BACKGROUND:Malaria caused by Plasmodium vivax is an experimentally neglected severe disease with a substantial burden on human health. Because of technical limitations, little is known about the biology of this important human pathogen. Whole genome analysis methods on patient-derived material are thus likely to have a...

journal_title：BMC genomics

authors： Bright AT,Tewhey R,Abeles S,Chuquiyauri R,Llanos-Cuentas A,Ferreira MU,Schork NJ,Vinetz JM,Winzeler EA

更新日期：2012-06-21 00:00:00