Abstract:
:Melamine detection has attracted much attention since the discovery of the damage of melamine to human health. Herein, we have developed a sensitive homogeneous electroanalytical platform for melamine detection, which is relied on the formation of triplex molecular beacon integrated with exonuclease III (Exo III)-mediated signal amplification. The formation of triplex molecular beacon was triggered by the recognition and incorporation of melamine to the abasic (AP) site contained in the triplex stem. The stem of the triplex molecular beacon was designed to have a protruding double-strand DNA, which can be recognized and hydrolyzed by Exo III for releasing methylene blue (MB)-labeled mononucleotide. These released MB molecules exhibit high diffusivity toward indium tin oxide electrode with negative charge, thus producing a significantly increased electrochemical response. Taking advantages of the high binding affinity of the DNA triplex structure containing AP sites towards melamine and the unique features of Exo III, this sensing platform is capable for sensitive and selective melamine assay with a detection limit as low as 8.7 nM. Furthermore, this strategy shows good applicability for melamine assay in real samples. Therefore, this strategy broadens the application of triplex DNA and presents a new method for sensitive detection of melamine.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Liu X,Song M,Li Fdoi
10.1038/s41598-017-04812-4subject
Has Abstractpub_date
2017-07-03 00:00:00pages
4490issue
1issn
2045-2322pii
10.1038/s41598-017-04812-4journal_volume
7pub_type
杂志文章abstract::Surface plasmon resonators can drastically redistribute incident light over different output wave vectors and polarizations. This can lead for instance to sub-diffraction sized nanoapertures in metal films that beam and to nanoparticle antennas that enable efficient conversion of photons between spatial modes, or heli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09966
更新日期:2015-04-30 00:00:00
abstract::CIC-DUX4 sarcoma (CDS) is a group of rare, mesenchymal, small round cell tumours that harbour the unique CIC-DUX4 translocation, which causes aberrant gene expression. CDS exhibits an aggressive course and poor clinical outcome, thus novel therapeutic approaches are needed for CDS treatment. Although patient-derived c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04967-0
更新日期:2017-07-05 00:00:00
abstract::Blast-induced traumatic brain injury has been associated with neurodegenerative and neuropsychiatric disorders. To date, although damage due to oxidative stress appears to be important, the specific mechanistic causes of such disorders remain elusive. Here, to determine the mechanical variables governing the tissue da...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28271-7
更新日期:2018-07-06 00:00:00
abstract::Myocardial ischemia/reperfusion (I/R) injury detrimentally alters the prognosis of patients undergoing revascularization after acute myocardial infarction. Our previous study demonstrated that NF-κB-induced autophagy plays a detrimental role in cardiac I/R injury using a rabbit myocardial I/R model. In this study, we ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37448
更新日期:2016-11-18 00:00:00
abstract::The adaptation of herbivorous insects to various host plants facilitates the spread and outbreak of many important invasive pests, however, the molecular mechanisms that underneath this process are poorly understood. In the past three decades, two species of the whitefly Bemisia tabaci complex, Middle East-Asia Minor ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12387-3
更新日期:2017-09-21 00:00:00
abstract::The implementation of HTS (high-throughput sequencing) approaches is rapidly changing our understanding of the lichen symbiosis, by uncovering high bacterial and fungal diversity, which is often host-specific. Recently, HTS methods revealed the presence of multiple photobionts inside a single thallus in several lichen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26947-8
更新日期:2018-06-05 00:00:00
abstract::The aim of the study was to determine damage severity of wood-destroying insects on logs stored in forest depots. The Bevan damage classification (BDC) system, developed in 1987, was utilized to determine damage severity in log depots in 21 locations throughout seven provinces in Turkey. Pheromone traps were placed in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70696-6
更新日期:2020-08-13 00:00:00
abstract::Septic shock is associated with multiple injuries to organs and tissues. These events may induce the regenerative response of adult stem cells. However, little is known about how endogenous stem cells are modulated by sepsis. This study analyzed the circulation of hematopoietic stem cells (HSCs), endothelial progenito...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/s41598-019-39772-4
更新日期:2019-03-01 00:00:00
abstract::Spatially resolved analysis of uranium (U) isotopes in small volumes of actinide-bearing materials is critical for a variety of technical disciplines, including earth and planetary sciences, environmental monitoring, bioremediation, and the nuclear fuel cycle. However, achieving subnanometer-scale spatial resolution f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48479-5
更新日期:2019-08-23 00:00:00
abstract::Decitabine has been found to have anti-metabolic and anti-tumor activities in various tumor cells. Recently, the use of decitabine in combination with other conventional therapies reportedly resulted in improved anti-tumor activity against various tumors. Ionizing radiation (IR) is widely used as a cancer treatment. D...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32470
更新日期:2016-09-27 00:00:00
abstract::Technological advances have made it possible to examine dental resin composites using 3D nanometer resolution. This investigation aims to characterize existing dental nano-hybrid and micro-hybrid resin composites through comparing and contrasting nano-computed tomography (nano-CT) with micro-CT and high-resolution SEM...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72599-y
更新日期:2020-09-23 00:00:00
abstract::Cryo-electron microscopy (cryo-EM) has become a leading technology for determining protein structures. Recent advances in this field have allowed for atomic resolution. However, predicting the backbone trace of a protein has remained a challenge on all but the most pristine density maps (<2.5 Å resolution). Here we in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60598-y
更新日期:2020-03-09 00:00:00
abstract::LiYF4:Eu nanophosphors with a single tetragonal phase are synthesized, and various strategies to enhance the Eu(3+) emission from the nanophosphors are investigated. The optimized Eu(3+) concentration is 35 mol%, and the red emission peaks due to the (5)D0 →(7)FJ (J = 1 and 2) transitions of Eu(3+) ions are further en...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07866
更新日期:2015-01-19 00:00:00
abstract::Abiotic emergence of ordered information stored in the form of RNA is an important unresolved problem concerning the origin of life. A polymer longer than 40-100 nucleotides is necessary to expect a self-replicating activity, but the formation of such a long polymer having a correct nucleotide sequence by random react...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58060-0
更新日期:2020-02-03 00:00:00
abstract::Hand size perceptual distortions characterize adult human cognition. Notwithstanding the importance of uncovering how hand size representation develops in humans, studies in this field are still at a preliminary stage. Indeed, it is yet to be understood whether hand size distortions are present and reliable in early c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62206-5
更新日期:2020-03-25 00:00:00
abstract::We study the spin-orbit interaction of two-dimensional electron/hole gas (2DEGs/2DHGs) on quasi-2D potassium niobates (KNs) via first-principles calculations. The strong surface polarity changes the free surface states from 2DEGs to 2DHGs. The in-plane dipole maintained on 2D models leads to giant Zeeman-type spin spl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60653-8
更新日期:2020-02-28 00:00:00
abstract::Inherited retinal dystrophy (IRD) patients often experience photophobia. However, its mechanism has not been elucidated. This study aimed to investigate the main wavelength of light causing photophobia in IRD and difference among patients with different phenotypes. Forty-seven retinitis pigmentosa (RP) and 22 cone-rod...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71707-2
更新日期:2020-09-09 00:00:00
abstract::Coherent, optically dressed media composed of two-level molecular systems without inversion symmetry are considered as all-optically tunable sources of coherent radiation in the microwave domain. A theoretical model and a numerical toolbox are developed to confirm the main finding: the generation of low-frequency radi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74569-w
更新日期:2020-10-19 00:00:00
abstract::Skin ageing is an inevitable consequence of life and accelerated by exposure to ultraviolet (UV) rays. Senescence is an irreversible growth arrest and senescent cells accumulate in ageing tissues, at sites of age-related pathologies and in pre-neoplastic lesions. Conventionally, senescent cells have been detected by s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15901-9
更新日期:2017-11-15 00:00:00
abstract::How microgravitational space environments affect aging is not well understood. We observed that, in Caenorhabditis elegans, spaceflight suppressed the formation of transgenically expressed polyglutamine aggregates, which normally accumulate with increasing age. Moreover, the inactivation of each of seven genes that we...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00487
更新日期:2012-01-01 00:00:00
abstract::Developing effective treatment strategies for neurodegenerative diseases require an understanding of the underlying cellular pathways that lead to neuronal vulnerability and progressive degeneration. To date, numerous mutations in 147 distinct genes are identified to be "associated" with, "modifier" or "causative" of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32902-4
更新日期:2018-10-03 00:00:00
abstract::Crosslinking is an effective way to fabricate high-selective CO2 separation membranes because of its unique crosslinking framework. Thus, it is essentially significant to study the influence of crosslinking degree on the permeation selectivities of CO2. Herein, we report a successful and facile synthesis of a series o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40082
更新日期:2017-01-04 00:00:00
abstract::Footprint evidence of human-megafauna interactions remains extremely rare in the archaeological and palaeontological records. Recent work suggests ancient playa environments may hold such evidence, though the prints may not be visible. These so-called "ghost tracks" comprise a rich archive of biomechanical and behavio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52996-8
更新日期:2019-11-11 00:00:00
abstract::AMPA subtype ionotropic glutamate receptors (iGluRs) mediate the majority of fast neurotransmission across excitatory synapses in the central nervous system. Each AMPA receptor is composed of four multi-domain subunits that are organized into layers of two amino-terminal domain (ATD) dimers, two ligand-binding domain ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19082
更新日期:2016-01-07 00:00:00
abstract::Although more than 1,000 androgen receptor (AR) mutations have been identified and these mutants are pathologically important, few theoretical studies have investigated the role of AR protein folding stability in disease and its relationship with the phenotype of the patients. Here, we extracted AR variant data from f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68731-7
更新日期:2020-07-21 00:00:00
abstract::Prestin in the lateral membrane of outer hair cells, is responsible for electromotility (EM) and a corresponding nonlinear capacitance (NLC). Prestin's voltage sensitivity is influenced by intracellular chloride. A regulator of intracellular chloride is a stretch-sensitive, non-selective conductance within the lateral...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46619
更新日期:2017-04-19 00:00:00
abstract::BEST1 is a Ca2+-activated Cl- channel predominantly expressed in retinal pigment epithelium (RPE), and over 250 genetic mutations in the BEST1 gene have been identified to cause retinal degenerative disorders generally known as bestrophinopathies. As most BEST1 mutations are autosomal dominant, it is of great biomedic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54892-7
更新日期:2019-12-13 00:00:00
abstract::Multidrug-resistant enterococcal pathogens, especially vancomycin-resistant enterococci (VRE), are among the pathogens that require new antibiotic innovation. The colonization of the gut represents a major pathway by which VRE can cause infection and spread to other patients. In the current study, auranofin (FDA-appro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26674-0
更新日期:2018-05-29 00:00:00
abstract::A fluorimetric Hg2+ test strip has been developed using a lotus-inspired fabrication method for suppressing the "coffee stains" toward the uniform distribution of probe materials through creating a hydrophobic drying pattern for fast solvent evaporation. The test strips were first loaded with the model probes of fluor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36494
更新日期:2016-11-04 00:00:00
abstract::The present study aimed to evaluate the efficiency, effectiveness, and biocompatibility of two agents used for the chemomechanical removal of carious dentin. Sixty extracted carious human teeth were treated with a conventional bur (CBG) or chemomechanical agents - Papacarie Duo (PG) and Brix 3000 (BG). Treatment effic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65159-x
更新日期:2020-05-15 00:00:00