Abstract：
:AMPA subtype ionotropic glutamate receptors (iGluRs) mediate the majority of fast neurotransmission across excitatory synapses in the central nervous system. Each AMPA receptor is composed of four multi-domain subunits that are organized into layers of two amino-terminal domain (ATD) dimers, two ligand-binding domain (LBD) dimers, transmembrane domains and carboxy-terminal domains. We introduced cysteine substitutions at the intersubunit interfaces of AMPA receptor subunit GluA2 and confirmed substituted cysteine crosslink formation by SDS-PAGE. The functional consequence of intersubunit crosslinks was assessed by recording GluA2-mediated currents in reducing and non-reducing conditions. Strong redox-dependent changes in GluA2-mediated currents were observed for cysteine substitutions at the LBD dimer-dimer interface but not at the ATD dimer-dimer interface. We conclude that during gating, LBD dimers undergo significant relative displacement, while ATD dimers either maintain their relative positioning, or their relative displacement has no appreciable effect on AMPA receptor function.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yelshanskaya MV,Saotome K,Singh AK,Sobolevsky AIdoi
10.1038/srep19082subject
Has Abstractpub_date
2016-01-07 00:00:00pages
19082issn
2045-2322pii
srep19082journal_volume
6pub_type
杂志文章abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-42219-5
更新日期：2019-04-16 00:00:00
abstract：:Loss of anorectal resting pressure due to internal anal sphincter (IAS) dysfunctionality causes uncontrolled fecal soiling and leads to passive fecal incontinence (FI). The study is focused on immediate and long-term safety and potential efficacy of bioengineered IAS BioSphincters to treat passive FI in a clinically r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54440-3
更新日期：2019-12-02 00:00:00
abstract：:Remote earthquake triggering is a well-established phenomenon. Triggering is commonly identified from statistically significant increases in earthquake rate coincident with the passage of seismic energy. In establishing rate changes, short duration earthquake catalogs are commonly used, and triggered sequences are not...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60988-2
更新日期：2020-03-05 00:00:00
abstract：:Urothelial carcinoma (UC) is a common disease causing significant morbidity and mortality as well as considerable costs for health systems. Extensive aberrant methylation of DNA is broadly documented in early UC, contributing to genetic instability, altered gene expression and tumor progression. However the triggers i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60796-8
更新日期：2020-03-02 00:00:00
abstract：:We performed a metabolomics study using liquid chromatography-mass spectrometry (LC-MS) combined with multivariate data analysis (MVDA) to discriminate global urine profiles in urine samples from esophageal squamous cell carcinoma (ESCC) patients and healthy controls (NC). Our work evaluated the feasibility of employi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35010
更新日期：2016-10-11 00:00:00
abstract：:Celiac disease (CeD) is a multifactorial disease influenced by both genetic and environmental risk factors. CeD genetic components are mainly due to HLA class II genes, which account for approximately 40% of the disease heritability. The environmental factor is linked to gliadin ingestion. Despite genetic and epigenet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50735-7
更新日期：2019-10-04 00:00:00
abstract：:Fungi play a key role in the functioning of soil in terrestrial ecosystems, and in particular in the remediation of degraded soils. The contribution of fungi to carbon and nutrient cycles, along with their capability to mobilise soil trace elements, is well-known. However, the importance of life history strategy for t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63194-2
更新日期：2020-04-15 00:00:00
abstract：:Passive immunotherapy with monoclonal antibodies (mAbs) is an efficacious treatment for Ebola virus (EBOV) infections in animal models and humans. Understanding what constitutes a protective response is critical for the development of novel therapeutic strategies. We generated an EBOV-glycoprotein-pseudotyped Human im...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45552
更新日期：2017-03-30 00:00:00
abstract：:We report, for the first time, the generation of high-order harmonics in a spectral range between 200 eV and 1 keV with an unusual spectral property: only every 4(th) (4i + 1, i∈ℵ) harmonic line appears, whereas the usual high-harmonic spectra consist of every odd (2i + 1) harmonic. We attribute this unique property t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04234
更新日期：2014-02-28 00:00:00
abstract：:Liquid repellent surfaces typically work by keeping the fouling liquid in a metastable state, with trapped pockets of air between the substrate and the liquid. An alternative method with greater long-term stability utilizes liquid-impregnated surfaces, where the liquid being repelled slides over an immiscible liquid i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06621-1
更新日期：2017-07-20 00:00:00
abstract：:Magnetic vortex is one of the simplest topologically non-trivial textures in condensed matter physics. It is the ground state of submicron magnetic elements (dots) of different shapes: cylindrical, square etc. So far, the vast majority of the vortex dynamics studies were focused on thin dots with thickness 5-50 nm and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13881
更新日期：2015-09-10 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep42627. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/srep46966
更新日期：2018-04-06 00:00:00
abstract：:The function of Forkhead box O 1 (FOXO1) and pSerine256-FOXO1 immunostaining in esophageal cancer is unclear. To clarify the prognostic role of nuclear FOXO1 and cytoplasmic pSerine256-FOXO1 immunostaining, a tissue microarray containing more than 600 esophageal cancers was analyzed. In non-neoplastic esophageal mucos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35459-4
更新日期：2018-11-26 00:00:00
abstract：:The gliotransmitter glutamate released from astrocytes can modulate neuronal firing by activating neuronal N-methyl-D-aspartic acid (NMDA) receptors. This enables astrocytic glutamate(AG) to be involved in neuronal physiological and pathological functions. Based on empirical results and classical neuron-glial "tripart...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32343
更新日期：2016-08-30 00:00:00
abstract：:Within the framework of precision agriculture, the determination of various soil properties is moving into focus, especially the demand for sensors suitable for in-situ measurements. Energy-dispersive X-ray fluorescence (EDXRF) can be a powerful tool for this purpose. In this study a huge diverse soil set (n = 598) fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53426-5
更新日期：2019-11-26 00:00:00
abstract：:We report that misfolded cytosolic proteins can be cleared from mammalian cells by directing them to endoplasmic reticulum (ER). NAT1 R64W and Parkin R42P are naturally occurring misfolded variants of cytosolic enzymes that acetylate arylamines and ubiquitinate proteins, respectively. We demonstrate that proteasome in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12088
更新日期：2015-07-14 00:00:00
abstract：:Caspase-2 is known to be involved in oxidative-stress mediated neuronal cell death. In this study, we demonstrated that rotenone-induced neuronal cell death is mediated by caspase-2 activation via PIDDosome formation. Our newly designed TAT-fused peptides, which contains wild-type helix number3 (H3) from RAIDD and PID...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31198
更新日期：2016-08-09 00:00:00
abstract：:Multi-junction solar cells made by assembling semiconductor materials with different bandgap energies have hold the record conversion efficiencies for many years and are currently approaching 50%. Theoretical efficiency limits make use of optimum designs with the right lattice constant-bandgap energy combination, whic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04321-4
更新日期：2017-06-21 00:00:00
abstract：:The poor bonding strength between biopolymer and bioceramic has remained an unsolved issue. In this study, graphene oxide (GO) was introduced as an interface phase to improve the interfacial bonding between polyetheretherketone (PEEK) and hydroxyapatite (HAP) for tissue engineering scaffolds. On the one hand, the conj...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46604
更新日期：2017-04-20 00:00:00
abstract：:Many remarkable properties of graphene are derived from its large energy window for Dirac-like electronic states and have been explored for applications in electronics and photonics. In addition, strong electron-phonon interaction in graphene has led to efficient photo-thermo energy conversions, which has been harness...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10582
更新日期：2015-06-08 00:00:00
abstract：:Objectum-sexuality (OS) is a sexual orientation which has received little attention in the academic literature. Individuals who identify as OS experience emotional, romantic and/or sexual feelings towards inanimate objects (e.g. a bridge, a statue). We tested 34 OS individuals and 88 controls, and provide the first em...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56449-0
更新日期：2019-12-27 00:00:00
abstract：:Seagrass meadows are threatened by coastal development and global change. In the face of these pressures, molecular techniques such as reverse transcription quantitative real-time PCR (RT-qPCR) have great potential to improve management of these ecosystems by allowing early detection of chronic stress. In RT-qPCR, the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17051
更新日期：2015-11-23 00:00:00
abstract：:The present study investigates the impact of charge variants on bevacizumab's structure, stability, and biological activity. Five basic and one acidic charge variants were separated using semi-preparative cation exchange chromatography using linear pH gradient elution with purity > 85%. Based on the commercial biosimi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79541-2
更新日期：2021-01-29 00:00:00
abstract：:Soluble starch synthase I (SSI) is a key enzyme in the biosynthesis of plant amylopectin. In this study, the gene named IbSSI, was cloned from sweet potato, an important starch crop. A high expression level of IbSSI was detected in the leaves and storage roots of the sweet potato. Its overexpression significantly incr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02481-x
更新日期：2017-05-24 00:00:00
abstract：:The Atacama Desert has been pointed out as one of the places on earth where the highest surface irradiance may occur. This area is characterized by its high altitude, prevalent cloudless conditions and relatively low columns of ozone and water vapor. Aimed at the characterization of the solar spectrum in the Atacama D...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22457
更新日期：2016-03-02 00:00:00
abstract：:In this decade coherent X-ray diffraction imaging has been demonstrated to reveal internal structures of whole biological cells and organelles. However, the spatial resolution is limited to several tens of nanometers due to the poor scattering power of biological samples. The challenge is to recover correct phase info...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08074
更新日期：2015-01-28 00:00:00
abstract：:When one liquid is introduced into another immiscible one, it ultimately fragments due to hydrodynamic instability. In contrast to neck pinch-off without external actuation, the viscous two-fluid system subjected to an oscillatory flow demonstrates higher efficiency in breaking fluid threads. However, the underlying d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31436
更新日期：2016-08-11 00:00:00
abstract：:The freshwater crustacean Daphnia is known for its ability to develop inducible morphological defences that thwart predators. These defences are developed only in the presence of predators and are realized as morphological shape alterations e.g. 'neckteeth' in D. pulex and 'crests' in D. longicephala. Both are discuss...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09649-5
更新日期：2017-08-29 00:00:00
abstract：:Circadian clocks are endogenous oscillators driving daily rhythms in physiology. The cell-autonomous clock is governed by an interlocked network of transcriptional feedback loops. Hundreds of clock-controlled genes (CCGs) regulate tissue specific functions. Transcriptome studies reveal that different organs (e.g. live...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05782
更新日期：2014-07-22 00:00:00
abstract：:Acute heart failure (AHF) emerges either de novo or from worsening of chronic heart failure (CHF). The aim of the present study was to evaluate the association between worsening of CHF and mortality in AHF patients. Out of 152 included AHF patients, 47 (30.9%) were de novo AHF patients and 105 (69%) were AHF patients ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28027-3
更新日期：2018-06-25 00:00:00
