A simple electroelution method for rapid protein purification: isolation and antibody production of alpha toxin from Clostridium septicum.

abstract：:The Somali Sengi or Somali Elephant-shrew ('Elephantulus' revoilii, Macroscelidea, Mammalia) has been considered a "lost species" and is primarily known from about 39 museum specimens, with no new vouchered occurrence records since the early 1970s. The scientific literature contains no data concerning living Somali Se...

journal_title：PeerJ

authors： Heritage S,Rayaleh H,Awaleh DG,Rathbun GB

更新日期：2020-08-18 00:00:00

abstract：:Soil microbial communities are continuously exposed to H2 diffusing into the soil from the atmosphere. N2-fixing nodules represent a peculiar microniche in soil where H2 can reach concentrations up to 20,000 fold higher than in the global atmosphere (0.530 ppmv). In this study, we investigated the impact of H2 exposur...

journal_title：PeerJ

authors： Piché-Choquette S,Tremblay J,Tringe SG,Constant P

更新日期：2016-03-10 00:00:00

abstract：:Synechococcus is among the most important contributors to global primary productivity. The genomes of several strains of this taxon have been previously sequenced in an effort to understand the physiology and ecology of these highly diverse microorganisms. Here we present a comparative study of Synechococcus genomes. ...

journal_title：PeerJ

authors： Coutinho F,Tschoeke DA,Thompson F,Thompson C

更新日期：2016-01-14 00:00:00

abstract：:Apis cerana cerana relies on its sensitive olfactory system to perform foraging activities in the surrounding environment. Olfactory receptors (ORs) are a primary requirement for odorant recognition and coding. However, the molecular recognition of volatile compounds with ORs in A. cerana cerana is still not clear. He...

journal_title：PeerJ

authors： Guo L,Zhao H,Jiang Y

更新日期：2018-06-11 00:00:00

abstract：:Consumer-grade GPS units are a staple of modern field ecology, but the relatively large error radii reported by manufacturers (up to 10 m) ostensibly precludes their utility in measuring fine-scale movement of small animals such as insects. Here we demonstrate that for data collected at fine spatio-temporal scales, th...

journal_title：PeerJ

authors： Breed GA,Severns PM

更新日期：2015-08-20 00:00:00

abstract：OBJECTIVES:Compare the immediate effects of a Neurodynamic Mobilization (NM) treatment or foam roller (FR) treatment after DOMS. DESIGN:Double blind randomised clinical trial. SETTING:The participants performed 100 drop jumps (5 sets of 20 repetitions, separated by 2 min rests) from a 0.5-m high box in a University b...

journal_title：PeerJ

authors： Romero-Moraleda B,La Touche R,Lerma-Lara S,Ferrer-Peña R,Paredes V,Peinado AB,Muñoz-García D

更新日期：2017-10-13 00:00:00

abstract：:Chemical immobilisation is an integral component for the conservation of wild animals and can be stressful if proper protocols are not administered. References on the immobilisation of Arabian striped hyaena (Hyaena hyaena sultana) are scarce. The current study was designed to evaluate the physiological and clinical r...

journal_title：PeerJ

authors： Mehmood A,Abid S,Hejcmanová P,Asadi MA,Kabeer B,Jilani MJ,Bilal S,Ashraf MW

更新日期：2019-07-26 00:00:00

abstract：:In this study, four experimental assays were conducted to evaluate the use of a new silver nanoparticle formulation named Argovit-4, which was prepared with slight modifications to enhance its biological activity against white spot syndrome virus (WSSV) in shrimp culture. The goals of these assays were to (1) determin...

journal_title：PeerJ

authors： Romo-Quiñonez CR,Álvarez-Sánchez AR,Álvarez-Ruiz P,Chávez-Sánchez MC,Bogdanchikova N,Pestryakov A,Mejia-Ruiz CH

更新日期：2020-02-27 00:00:00

abstract：:Background. The ReproQuestionnaire (ReproQ) measures the client's experience with maternity care, following the WHO responsiveness model. In 2015, the ReproQ was appointed as national client experience questionnaire and will be added to the national list of indicators in maternity care. For using the ReproQ in quality...

journal_title：PeerJ

authors： Scheerhagen M,van Stel HF,Tholhuijsen DJ,Birnie E,Franx A,Bonsel GJ

更新日期：2016-07-13 00:00:00

abstract：:Background. The gram-negative Neisseria is associated with two of the most potent human epidemic diseases: meningococcal meningitis and gonorrhoea. In both cases, disease is caused by bacteria colonizing human mucosal membrane surfaces. Overall, the genus shows great diversity and genetic variation mainly due to its a...

journal_title：PeerJ

authors： Zheng W,Mutha NV,Heydari H,Dutta A,Siow CC,Jakubovics NS,Wee WY,Tan SY,Ang MY,Wong GJ,Choo SW

更新日期：2016-03-17 00:00:00

abstract：Background:Although the postural instability accompanying bilateral vestibular loss in human and quadrupeds during lateral head movements are well-known, it is still unclear whether or not lateral head turns would indeed activate the postural control system to maintain balance. This study aimed to examine the kinetic p...

journal_title：PeerJ

authors： Song Y,Wang M,Baker JS,Gu Y

更新日期：2019-12-12 00:00:00

abstract：BACKGROUND:Constant reviews of the caring behavior of nurses and patient satisfaction help to improve the quality of nursing. The aim of our research was to explore relationships between the level of nursing education, the perception of nurses and nursing assistants of Watson's carative factors, and patient satisfactio...

journal_title：PeerJ

authors： Pajnkihar M,Štiglic G,Vrbnjak D

更新日期：2017-02-07 00:00:00

abstract：:The goal of this study was to assess the diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis. Blood samples were collected from 56 sheep (36 naturally infected with Pasteurella multocida and 20 healthy controls) belonging to one farm in Eastern region, Saudi...

journal_title：PeerJ

authors： El-Deeb WM,Elmoslemany AM

更新日期：2016-07-19 00:00:00

abstract：BACKGROUND:Self-contained tests estimate and test the association between a phenotype and mean expression level in a gene set defined a priori. Many self-contained gene set analysis methods have been developed but the performance of these methods for phenotypes that are continuous rather than discrete and with multiple...

journal_title：PeerJ

authors： Walker JA

更新日期：2016-10-11 00:00:00

abstract：:For the past several centuries, millipede taxonomists have used the morphology of male copulatory structures (modified legs called gonopods), which are strongly variable and suggestive of species-level differences, as a source to understand taxon relationships. Millipedes in the family Xystodesmidae are blind, dispers...

journal_title：PeerJ

authors： Means JC,Marek PE

更新日期：2017-10-12 00:00:00

abstract：:Mutations that cause an error in the splicing of a messenger RNA (mRNA) can lead to diseases in humans. Various computational models have been developed to recognize the sequence pattern of the splice sites. In recent studies, Convolutional Neural Network (CNN) architectures were shown to outperform other existing mod...

journal_title：PeerJ

authors： Thanapattheerakul T,Engchuan W,Chan JH

更新日期：2020-07-06 00:00:00

abstract：Background:The spread of maize cultivation to the highlands of central Mexico was accompanied by substantial introgression from the endemic wild teosinte Zea mays ssp. mexicana, prompting the hypothesis that the transfer of beneficial variation facilitated local adaptation. Methods:We used whole-genome sequence data t...

journal_title：PeerJ

authors： Gonzalez-Segovia E,Pérez-Limon S,Cíntora-Martínez GC,Guerrero-Zavala A,Janzen GM,Hufford MB,Ross-Ibarra J,Sawers RJH

更新日期：2019-05-03 00:00:00

abstract：Background:Much of the shape variation found in animals is based on allometry and heterochrony. Horses represent an excellent model to investigate patterns of size-shape variation among breeds that were intentionally bred for extreme small and large sizes. Methods:We tested whether ponies (wither height < 148 cm) have...

journal_title：PeerJ

authors： Heck L,Sanchez-Villagra MR,Stange M

更新日期：2019-09-16 00:00:00

abstract：:The eukaryotic polypyrimidine tract binding protein (PTB) serves primarily as a regulator of alternative splicing of messenger RNA, but is also co-opted to other roles such as RNA localisation and translation initiation from internal ribosome entry sites. The neuronal paralogue of PTB (nPTB) is 75% identical in amino ...

journal_title：PeerJ

authors： Joshi A,Esteve V,Buckroyd AN,Blatter M,Allain FH,Curry S

更新日期：2014-03-13 00:00:00

abstract：:Previous research has shown that odorants consistently evoke associations with textures and their tactile properties like smoothness and roughness. Also, it has been observed that olfaction can modulate tactile perception. We therefore hypothesized that tactile roughness perception may be biased towards the somatosens...

journal_title：PeerJ

authors： Koijck LA,Toet A,Van Erp JB

更新日期：2015-05-12 00:00:00

abstract：Background:The fecal microbiota from obese individuals can induce obesity in animal models. In addition, studies in humans, animal models and dogs have revealed that the fecal microbiota of subjects with obesity is different from that of lean subjects and changes after weight loss. However, the impact of weight loss on...

journal_title：PeerJ

authors： Bermudez Sanchez S,Pilla R,Sarawichitr B,Gramenzi A,Marsilio F,Steiner JM,Lidbury JA,Woods GRT,German AJ,Suchodolski JS

更新日期：2020-10-05 00:00:00

abstract：:In a number of recent studies we used computer modeling to investigate the evolution of muscle leverage (moment arms) and function in extant and extinct archosaur lineages (crocodilians, dinosaurs including birds and pterosaurs). These studies sought to quantify the level of disparity and convergence in muscle moment ...

journal_title：PeerJ

authors： Bates K,Maidment SC,Schachner ER,Barrett PM

更新日期：2015-10-08 00:00:00

abstract：Background:The coral genus Acropora contains more than 150 species with very high morphological diversity. This high diversity may have been caused by repeated hybridization via mass spawning. However, we have little information whether hybrids are formed in these corals. Identifying morphological differences between h...

journal_title：PeerJ

authors： Fukami H,Iwao K,Kumagai NH,Morita M,Isomura N

更新日期：2019-02-19 00:00:00

abstract：:Structural variants (SVs) are an important class of genetic variation implicated in a wide array of genetic diseases including cancer. Despite the advances in whole genome sequencing, comprehensive and accurate detection of SVs in short-read data still poses some practical and computational challenges. We present sv-c...

journal_title：PeerJ

authors： Kuzniar A,Maassen J,Verhoeven S,Santuari L,Shneider C,Kloosterman WP,de Ridder J

更新日期：2020-01-06 00:00:00

abstract：:We point out complications inherent in biodiversity inventory metrics when applied to large-scale datasets. The number of units of inventory effort (e.g., days of inventory effort) in which a species is detected saturates, such that crucial numbers of detections of rare species approach zero. Any rare errors can then ...

journal_title：PeerJ

authors： Khalighifar A,Jiménez L,Nuñez-Penichet C,Freeman B,Ingenloff K,Jiménez-García D,Peterson T

更新日期：2020-05-13 00:00:00

abstract：:Insect pollinators provide an essential ecosystem service by transferring pollen to crops and native vegetation. The extent to which pollinator communities vary both spatially and temporally has important implications for ecology, conservation and agricultural production. However, understanding the complex interaction...

journal_title：PeerJ

authors： Willcox BK,Robson AJ,Howlett BG,Rader R

更新日期：2018-10-19 00:00:00

abstract：Background:Recently, SARS-CoV-2 virus with the D614G mutation has become a public concern due to rapid dissemination of this variant across many countries. Our study aims were (1) to report full-length genome sequences of SARS-CoV-2 collected from four COVID-19 patients in the Special Region of Yogyakarta and Central J...

journal_title：PeerJ

authors： Gunadi,Wibawa H,Marcellus,Hakim MS,Daniwijaya EW,Rizki LP,Supriyati E,Nugrahaningsih DAA,Afiahayati,Siswanto,Iskandar K,Anggorowati N,Kalim AS,Puspitarani DA,Athollah K,Arguni E,Nuryastuti T,Wibawa T

更新日期：2020-12-21 00:00:00

abstract：:In this paper, we propose a method for automatically detecting the times during which game players exhibit specific behavior, such as when players commonly show excitement, concentration, immersion, and surprise. The proposed method detects such outlying behavior based on the game players' characteristics. These chara...

journal_title：PeerJ

authors： Kim YB,Kang SJ,Lee SH,Jung JY,Kam HR,Lee J,Kim YS,Lee J,Kim CH

更新日期：2015-12-10 00:00:00

abstract：:The number of people suffering from eye fatigue induced by visual display terminal (VDT) viewing is expected to increase in the modern world. Eye dysfunction is suggested to induce a decrease in cognitive function, at least in the long term. Furthermore, considering other previous findings, it may be reasonable to thi...

journal_title：PeerJ

authors： Akagi R,Tonotsuka M,Horie R,Hirata K,Ando S

更新日期：2019-10-29 00:00:00

abstract：:The Yazoo Darter, Etheostoma raneyi (Percidae), is an imperiled freshwater fish species endemic to tributaries of the Yocona and Little Tallahatchie rivers of the upper Yazoo River basin, in northern Mississippi, USA. The two populations are allopatric, isolated by unsuitable lowland habitat between the two river drai...

journal_title：PeerJ

authors： Sterling KA,Nielsen SV,Brown AJ,Warren ML Jr,Noonan BP

更新日期：2020-05-04 00:00:00