Persistence of antibiotic resistance plasmids in bacterial biofilms.

Abstract:

:The emergence and spread of antibiotic resistance is a crisis in health care today. Antibiotic resistance is often horizontally transferred to susceptible bacteria by means of multidrug resistance plasmids that may or may not persist in the absence of antibiotics. Because bacterial pathogens often grow as biofilms, there is a need to better understand the evolution of plasmid persistence in these environments. Here we compared the evolution of plasmid persistence in the pathogen Acinetobacter baumannii when grown under antibiotic selection in biofilms versus well-mixed liquid cultures. After 4 weeks, clones in which the plasmid was more stably maintained in the absence of antibiotic selection were present in both populations. On average plasmid persistence increased more in liquid batch cultures, but variation in the degree of persistence was greater among biofilm-derived clones. The results of this study show for the first time that the persistence of MDR plasmids improves in biofilms.

journal_name

Evol Appl

authors

Ridenhour BJ,Metzger GA,France M,Gliniewicz K,Millstein J,Forney LJ,Top EM

doi

10.1111/eva.12480

subject

Has Abstract

pub_date

2017-05-08 00:00:00

pages

640-647

issue

6

issn

1752-4571

pii

EVA12480

journal_volume

10

pub_type

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