Abstract:
:Allele-specific expression is traditionally studied by bulk RNA sequencing, which measures average expression across cells. Single-cell RNA sequencing allows the comparison of expression distribution between the two alleles of a diploid organism and the characterization of allele-specific bursting. Here, we propose SCALE to analyze genome-wide allele-specific bursting, with adjustment of technical variability. SCALE detects genes exhibiting allelic differences in bursting parameters and genes whose alleles burst non-independently. We apply SCALE to mouse blastocyst and human fibroblast cells and find that cis control in gene expression overwhelmingly manifests as differences in burst frequency.
journal_name
Genome Bioljournal_title
Genome biologyauthors
Jiang Y,Zhang NR,Li Mdoi
10.1186/s13059-017-1200-8subject
Has Abstractpub_date
2017-04-26 00:00:00pages
74issue
1eissn
1474-7596issn
1474-760Xpii
10.1186/s13059-017-1200-8journal_volume
18pub_type
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