Abstract:
:The therapeutic effect of citrate on alkali-burned eyes is thought to be the result of inhibition of PMN activities while ascorbate apparently stimulates the production of new collagen by scorbutic fibroblasts. Attachment of PMNs to the vascular endothelium is the initial PMN response to mediators and must occur before diapedesis and chemotaxis can produce a tissue infiltrate. The present study was undertaken to test the effects of citrate or ascorbate on the in vitro adherence of resting and leukotriene B4 (LTB4)-treated PMNs. Citrate preincubation of resting or LTB4-treated PMNs inhibited adherence in a dose-dependent manner. Inhibition of LTB4 augmented adherence by 12 mM citrate was effectively reversed by the addition of Ca2+ and Mg2+ (6 mM each). Ascorbate had no significant effect on the adherence of resting or LTB4 treated PMNs. In addition we wished to test the effectiveness of the breakdown product(s) from alkali burned collagen (Type 1) as an augmentor of adherence because of its effectiveness as a locomotory and respiratory burst stimulant. These break-down product(s) had no effect on the adherence of resting or LTB4-treated PMNs unless bovine serum albumin (BSA) was omitted from the incubation mixture. In the absence of BSA, these breakdown products inhibited both types of adherence in a dose-dependent manner with dilutions from 1:10 to 1:1000. This inhibition is probably not clinically meaningful since PMN adherence takes place in the vasculature where BSA would always be present. As a consequence of citrate inhibition of adherence in vivo, PMNs would be denied access to the injury site, trapping them in a resting state within the circulatory system.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Pfister RR,Haddox JL,Snyder TLsubject
Has Abstractpub_date
1988-06-01 00:00:00pages
869-75issue
6eissn
0146-0404issn
1552-5783journal_volume
29pub_type
杂志文章abstract:PURPOSE:To provide a clinical and genetic description of a patient cohort with Stargardt disease (STGD1) with identifiable foveal sparing. METHODS:Patients with retinal atrophy (defined as an absence of autofluorescence) that surrounded the fovea by at least 180° and did not include the fovea were defined as having fo...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-13825
更新日期:2014-10-16 00:00:00
abstract:PURPOSE:Weak eye muscles can cause ophthalmic disorders, and in particular, strabismus. Exogenous trophic factors such as cardiotrophin (CT)-1 and insulin-like growth factor (IGF) have been shown to increase the contractile force of adult heart and skeletal muscles, respectively. In the current study, the effects of en...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0393
更新日期:2004-10-01 00:00:00
abstract:PURPOSE:Downregulation of melanoma-associated antigens (MAAs), against which natural cytolytic T lymphocytes (CTLs) exist in humans, is one of the mechanisms that aids in evasion of immune surveillance. In view of putative re-expression strategies for MAAs during immunotherapy, this study was conducted to investigate M...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0961
更新日期:2005-01-01 00:00:00
abstract:Purpose:To compare static rod function obtained with and without photobleach in control and intermediate age-related macular degeneration (iAMD) participants with and without subretinal drusenoid deposits (SDD). Methods:In this cross-sectional study, retinal sensitivities within the central 24° retina were obtained tw...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-23970
更新日期:2018-08-01 00:00:00
abstract:PURPOSE:To test the hypothesis that retinal hypoxia is present during vascular development in normal rat pups and in a newborn rat model of retinopathy of prematurity (ROP). METHODS:Preretinal vitreous PO(2) measurements were made during room air breathing using (19)F magnetic resonance spectroscopy (MRS) and a perflu...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-1122
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:The prevalence of cataract increases with age. Conversely, the abundance of native α-crystallin diminishes with age and cataract development. We hypothesize replenishing lens α-crystallin may delay or prevent cataract. Herein we investigated the ability of cell penetration peptides (CPP) to enhance entry of α-c...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-10947
更新日期:2013-01-02 00:00:00
abstract:Purpose:To investigate by optical coherence tomography angiography (OCT-A) the choroidal vascular response to experimentally increased blood pressure in patients with central serous chorioretinopathy (CSCR). Methods:For this multicenter, observational, case-control study, we enrolled 35 patients with an established di...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
doi:10.1167/iovs.18-23995
更新日期:2018-08-01 00:00:00
abstract:PURPOSE:Oxidative stress has been proposed as a major pathogenic factor in age-related macular degeneration (AMD), the leading cause of vision loss among elderly people of western European ancestry. Lutein (LUT) and zeaxanthin (ZEA), major components in macular pigment, are among the retinal antioxidants. Though xantho...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.07-0037
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:To explore the effect of lateral tension as a survival factor for retinal explants in vitro. The central nervous system (CNS) resides in a highly mechanical milieu. However, the importance of biomechanical homeostasis for normal CNS function has not been extensively explored. Diseases in which normal mechanical...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-11420
更新日期:2013-03-13 00:00:00
abstract:Purpose:To investigate the molecular mechanisms of the antiangiogenic activity of UPARANT, an antagonist of the urokinase-type plasminogen activator receptor (uPAR), on primary human retinal endothelial cells (HREC) as a model of in vitro angiogenesis. Methods:The antiangiogenic activity of UPARANT was evaluated on en...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-19909
更新日期:2016-10-01 00:00:00
abstract::Gross correlation coefficient between light-scattering intensity and the optical parameters obtained for 48 sections of eight lenses with nuclear cataracts were evaluated. On the basis of these and other data in the literature, the structural elements, within the lens fiber are identified. These give rise to the optic...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract:PURPOSE:To investigate whether signaling pathways that incorporate protein tyrosine kinases and phosphatases regulate PKC-sensitive, volume-sensitive Cl(-) currents (I(Cl,vol)) in cultured rabbit nonpigmented ciliary epithelial cells. METHODS:Activation of I(Cl,vol) in response to hyposmotic stimulation was recorded w...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract:PURPOSE:Endothelin (ET)-1 is known to inhibit active NaK transport by as much as 50% in kidney tubule and other tissues. The presence of low levels of ET-1 in aqueous humor combined with the potential for release of ET-1 from ciliary processes suggests that the lens could be exposed to ET-1 in vivo. In this study, expe...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract:PURPOSE:We investigated the mechanism of tamoxifen (TAM) retinotoxicity using human retinal pigment epithelial (RPE)-derived (ARPE-19) and photoreceptor-derived (661W) cells. METHODS:Cultured ARPE-19 and 661W cells were treated with 5 to 10 μM TAM, and the resultant cell death was quantified using lactate dehydrogenas...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-9827
更新日期:2012-08-09 00:00:00
abstract:PURPOSE:This was a pilot study conducted to examine the expression of osteopontin in uveal melanoma and to determine whether serum osteopontin can be used in detecting metastatic uveal melanoma. METHODS:Osteopontin mRNA was measured in three uveal melanoma cell lines of various invasive potential by real-time PCR. Tis...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.05-0422
更新日期:2006-03-01 00:00:00
abstract:PURPOSE:To evaluate the contribution that fixation errors make to the overall variability of perimetric responses in patients with glaucoma. METHODS:Frequency of seeing curves were established, with and without fixation error correction, at two locations in each of 14 patients with glaucoma and good visual acuity. One...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract:PURPOSE:To detect the expression of cell adhesion molecules (CAMs), including beta integrins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1, leukocyte-endothelial cell adhesion molecule-2, E-cadherin, and CD44, in cultured lens epithelial cells (LECs) of human cataracts. To show that LEC...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::The cellular and subcellular distribution of S-100 protein was investigated at the ultrastructural level in the rat retina by the immunocytochemical PAP method. S-100 appeared to be localized in the cytoplasm and nucleus of Müller cells, offering conclusive evidence that in the mammalian retina, the protein is confine...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract:PURPOSE:To describe the in vivo evolution of laser-induced choroidal neovascularization (CNV) in mice using spectral domain optical coherence tomography (SD-OCT). METHODS:Laser photocoagulation was applied to the mouse fundus using a 532-nm diode laser (100, 150, and 200 mW; 100-μm diameter, 0.1-second duration). SD-O...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.10-6266
更新日期:2011-06-01 00:00:00
abstract::Recently protease inhibitors were used to treat corneal ulceration and persistent epithelial defect. An organ culture system was used in this study to examine the effect of four protease inhibitors on rat corneal epithelial migration and on incorporation of 3H-leucine into trichloroacetic acid-precipitable material as...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract:PURPOSE:To investigate the dynamic effect of tear lipocalins (TLs), the major lipid-binding protein in tears, at aqueous-cornea and lipid-aqueous interfaces, and their potential contribution to surface tension in the tear film. METHODS:Human apo- and holo-TLs were applied to the aqueous subphase in a Langmuir trough, ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract:PURPOSE:To test whether triamcinolone acetonide (TA) inhibits angiogenesis induced by IL-6 or VEGF and whether this inhibition is through antagonism of the IL-6 or the VEGF receptor 2. METHODS:A rat cornea micropocket assay was used to initiate IL-6- and VEGF-mediated angiogenesis. The ability of TA or neutralizing VE...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.05-1651
更新日期:2006-11-01 00:00:00
abstract:PURPOSE:We report on instantaneous volumetric flow rate of vitreous cutters measured by means of particle image velocimetry (PIV). METHODS:In an in vitro experimental study, vitreous cutters mounting a regular blade (RB) or modified Twedge blade (TB) engineered for higher flow were connected to a console machine equip...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.14-15467
更新日期:2014-11-20 00:00:00
abstract:Purpose:Prospective treatments for age-related macular degeneration and inherited retinal degenerations are commonly evaluated in the Royal College of Surgeons (RCS) rat before translation into clinical application. Historically, retinal thickness obtained through postmortem anatomic assessments has been a key outcome ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-20363
更新日期:2017-03-01 00:00:00
abstract:Purpose:Light-emitting diodes that emit high-intensity blue light are associated with blue-light hazard. Here, we report that blue light disturbs circadian rhythms by interfering with the clock gene in the suprachiasmatic nucleus (SCN) and that suppression of blue light at night ameliorates metabolic abnormalities by c...
journal_title:Investigative ophthalmology & visual science
pub_type: 临床试验,杂志文章
doi:10.1167/iovs.19-27195
更新日期:2019-09-03 00:00:00
abstract:PURPOSE:To determine whether tonometric readings of increases in intraocular pressure (IOP) during the water-drinking test (WDT) are affected by variations in central corneal thickness (CCT) induced by photorefractive keratectomy (PRK). METHODS:Data from 30 randomly selected eyes of 30 patients (18 men and 12 women; m...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-1240
更新日期:2005-09-01 00:00:00
abstract:PURPOSE:The chaperone proteins, α-crystallins, also possess antiapoptotic properties. The purpose of the present study was to investigate whether 19 to 20-mer α-crystallin-derived mini-chaperone peptides (α-crystallin mini-chaperone) are antiapoptotic, and to identify their putative transporters in human fetal RPE (hfR...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-11571
更新日期:2013-04-17 00:00:00
abstract:PURPOSE:To mathematically analyze and to clinically describe specific anatomic foveal configurations predisposing to the formation of macular holes in comparison with normal foveal anatomy. METHODS:In a retrospective observational case-control series, a total of 3882 optical coherence tomography (OCT) foveal thickness...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-8191
更新日期:2011-10-21 00:00:00
abstract:PURPOSE:MRL/MpJ-+/+ (MRL/+) and MRL/MpJ-lpr/lpr (MRL/lpr) mice show spontaneous development of a T-cell-driven lacrimal gland inflammation that is a model for Sjögren syndrome. The lacrimal gland lesions in these mice were evaluated by quantitative RT-PCR for selected cytokine mRNA for the relative contributions of T-h...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
abstract:PURPOSE:Familial exudative vitreoretinopathy (FEVR) is a rare hereditary disorder characterized by the failure of peripheral retinal vascularization. The genes FZD4, LRP5, and TSPAN12 are known to be associated with the autosomal inheritance form of FEVR. In this study, we performed mutation screening for FZD4, LRP5, a...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.14-15680
更新日期:2015-08-01 00:00:00