当前位置： SCI文献检索 > INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE期刊下所有文献 > Induction of autophagy and cell death by tamoxifen in cultured retinal pigment epithelial and photoreceptor cells.

Induction of autophagy and cell death by tamoxifen in cultured retinal pigment epithelial and photoreceptor cells.

Abstract：

PURPOSE:We investigated the mechanism of tamoxifen (TAM) retinotoxicity using human retinal pigment epithelial (RPE)-derived (ARPE-19) and photoreceptor-derived (661W) cells. METHODS:Cultured ARPE-19 and 661W cells were treated with 5 to 10 μM TAM, and the resultant cell death was quantified using lactate dehydrogenase (LDH) release assay. Cellular oxidative stress was determined by measuring 5-(and-6)-carboxy-2',7'-dichlorohydrofluorescein diacetate (H(2)-DCFDA) fluorescence. Changes in intracellular free zinc levels were monitored using the zinc-specific fluorescent dye, FluoZin-3 AM. Autophagic vacuole formation was assessed morphologically in ARPE-19 and 661W cells transfected with the fluorescent protein-conjugated markers, RFP-LC3 or GFP-LC3. RESULTS:Following exposure to TAM, both ARPE-19 and 661W cells had cytosolic vacuoles within 1 hour and underwent cell death within 18 hours. In both cell types, TAM-induced cell death was accompanied by increased oxidative stress and elevated zinc levels, and was attenuated by the antioxidant N-acetyl-L-cysteine (NAC) or the zinc chelator N,N,N'N'-tetrakis(-)(2-pyridylmethyl)-ethylenediamine (TPEN). The levels of LC3-II as well as the number of autophagic vacuoles (AVs) increased after TAM treatment. Double staining for lysosomes and AVs showed that autolysosome formation proceeded normally. Consistent with this, autophagy flux was increased. Finally, as shown in other cases of autophagic cell death, lysosomal membrane permeabilization (LMP) as well as caspase-dependent apoptosis contributed to TAM-induced cell death. CONCLUSIONS:ARPE-19 and 661W cells were vulnerable similarly to TAM-induced cytotoxicity. Increases in zinc levels and oxidative stress, excessive activation of autophagy flux, and ultimately the occurrence of LMP and consequent caspase activation may contribute to the well-established retinal cytotoxicity of TAM.

journal_name

Invest Ophthalmol Vis Sci

journal_title

Investigative ophthalmology & visual science

authors

Cho KS,Yoon YH,Choi JA,Lee SJ,Koh JY

doi

10.1167/iovs.12-9827

subject

Has Abstract

pub_date

2012-08-09 00:00:00

pages

5344-53

issue

9

eissn

0146-0404

issn

1552-5783

pii

iovs.12-9827

journal_volume

53

pub_type

杂志文章

相关文献

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE文献大全
  • Extracellular nucleotides and interleukin-8 production by ARPE cells: potential role of danger signals in blood-retinal barrier activation.

    abstract：PURPOSE:RPE cell activation is an important feature of autoimmune uveitis. This investigation focused on whether extracellular nucleotides could contribute to this activation, and the effects of ATPgammaS, UTP, and UDP on the production of IL-8 by RPE cells was studied in relation to their expression of functional P2Y ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.08-1902

    authors： Relvas LJ,Bouffioux C,Marcet B,Communi D,Makhoul M,Horckmans M,Blero D,Bruyns C,Caspers L,Boeynaems JM,Willermain F

    更新日期：2009-03-01 00:00:00

  • Characterizing the Natural History of Visual Function in Choroideremia Using Microperimetry and Multimodal Retinal Imaging.

    abstract：Purpose:Centripetal retinal degeneration in choroideremia (CHM) leads to early visual field restriction and late central vision loss. The latter marks an acute decline in quality of life but visual prognostication remains challenging. We investigated visual function in CHM by correlating best-corrected visual acuity (B...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,随机对照试验

    doi：10.1167/iovs.17-22486

    authors： Jolly JK,Xue K,Edwards TL,Groppe M,MacLaren RE

    更新日期：2017-10-01 00:00:00

  • A comprehensive survey of mutations in the OPA1 gene in patients with autosomal dominant optic atrophy.

    abstract：PURPOSE:To characterize the spectrum of mutations in the OPA1 gene in a large international panel of patients with autosomal dominant optic atrophy (adOA), to improve understanding of the range of functional deficits attributable to sequence variants in this gene, and to assess any genotype-phenotype correlations. MET...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究

    doi：

    authors： Thiselton DL,Alexander C,Taanman JW,Brooks S,Rosenberg T,Eiberg H,Andreasson S,Van Regemorter N,Munier FL,Moore AT,Bhattacharya SS,Votruba M

    更新日期：2002-06-01 00:00:00

  • Carbonic Anhydrase Inhibitors for the Treatment of Cystic Macular Lesions in Children With X-Linked Juvenile Retinoschisis.

    abstract：Purpose:Little is known regarding the therapeutic effect of carbonic anhydrase inhibitors (CAIs) in the management of cystic macular lesions in children with X-linked juvenile retinoschisis (XLRS) despite the fact that this disease often manifests during childhood. Therefore, our goal was to determine the efficacy of C...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-20078

    authors： Verbakel SK,van de Ven JP,Le Blanc LM,Groenewoud JM,de Jong EK,Klevering BJ,Hoyng CB

    更新日期：2016-10-01 00:00:00

  • Expression of CD40 and CD40 ligand in the human conjunctival epithelium.

    abstract：PURPOSE:CD40 antigen is a membrane receptor that plays a role in the regulation of immune reactions. The expressions of CD40 and CD40 ligand (CD40L) were investigated ex vivo and in vitro in conjunctival epithelial cells, in correlation with HLA DR class H antigen, previously shown to be upregulated in conjunctival inf...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Bourcier T,De Saint-Jean M,Brignole F,Goguel A,Baudouin C

    更新日期：2000-01-01 00:00:00

  • Increased Indoleamine 2,3-Dioxygenase and Quinolinic Acid Expression in Microglia and Müller Cells of Diabetic Human and Rodent Retina.

    abstract：Purpose:We investigated the relationship between inflammation, neuronal loss, and expression of indoleamine 2, 3-dioxygenase (IDO) and quinolinic acid (QUIN) in the retina of subjects with type 1 diabetes (T1D) and type 2 diabetes (T2D) and in the retina of rats with T1D. Methods:Retinas from T1D (n = 7), T2D (n = 13)...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-21654

    authors： Hu P,Hunt NH,Arfuso F,Shaw LC,Uddin MN,Zhu M,Devasahayam R,Adamson SJ,Benson VL,Chan-Ling T,Grant MB

    更新日期：2017-10-01 00:00:00

  • Outer Retinal Changes Including the Ellipsoid Zone Band in Usher Syndrome 1B due to MYO7A Mutations.

    abstract：PURPOSE:To study transition zones from normal to abnormal retina in Usher syndrome IB (USH1B) caused by myosin 7A (MYO7A) mutations. METHODS:Optical coherence tomography (OCT) scattering layers in outer retina were segmented in patients (n = 16, ages 2-42; eight patients had serial data, average interval 4.5 years) to...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-18860

    authors： Sumaroka A,Matsui R,Cideciyan AV,McGuigan DB 3rd,Sheplock R,Schwartz SB,Jacobson SG

    更新日期：2016-07-01 00:00:00

  • Defects in cortisol-metabolizing enzymes in primary open-angle glaucoma.

    abstract：:Assays of cortisol-metabolizing enzymes in homogenates of human trabecular meshwork cells under optimal conditions revealed two defects in primary open-angle glaucoma (POAG): one is a marked increase in delta 4-reductase and the other is a decrease in 3-oxidoreductase. Experiments indicated that the differences in enz...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Weinstein BI,Munnangi P,Gordon GG,Southren AL

    更新日期：1985-06-01 00:00:00

  • Regulation of paracrine cytokine balance controlling collagenase synthesis by corneal cells.

    abstract：PURPOSE:Classic studies have demonstrated that corneal epithelial cell density in culture can alter the balance of stimulatory and inhibitory cytokines controlling the elaboration of collagenolytic activity by co-cultured stromal cells. The current study attempts to bring the understanding of this mechanism to a molecu...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Strissel KJ,Rinehart WB,Fini ME

    更新日期：1997-02-01 00:00:00

  • Signaling states of rhodopsin in rod disk membranes lacking transducin βγ-complex.

    abstract：PURPOSE:To characterize the possible role of transducin Gtβγ-complex in modulating the signaling properties of photoactivated rhodopsin and its lifetime in rod disc membranes and intact rods. METHODS:Rhodopsin photolysis was studied using UV-visible spectroscopy and rapid scanning spectroscopy in the presence of hydro...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.11-9350

    authors： Lomonosova E,Kolesnikov AV,Kefalov VJ,Kisselev OG

    更新日期：2012-03-09 00:00:00

  • Characteristics of Retinal Nerve Fiber Layer Defect in Nonglaucomatous Eyes With Type II Diabetes.

    abstract：PURPOSE:To investigate the characteristics of retinal nerve fiber layer (RNFL) defects associated with type II diabetes. METHODS:Forty nonglaucomatous eyes with type II diabetes and 54 eyes with early open angle glaucoma that exhibited a localized RNFL defect and 42 eyes from age- and sex-matched nondiabetic, nonglauc...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-19525

    authors： Jeon SJ,Kwon JW,La TY,Park CK,Choi JA

    更新日期：2016-08-01 00:00:00

  • Spasmus nutans. A long-term follow-up.

    abstract：PURPOSE:Nystagmus, head nodding, and anomalous head position are symptoms of spasmus nutans. This disorder appears in early childhood and is thought to be self-limited. However, the visual outcome of patients with spasmus nutans is unclear. The resolution of nystagmus has not been proven with quantitative eye movement ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Gottlob I,Wizov SS,Reinecke RD

    更新日期：1995-12-01 00:00:00

  • The neurologic evaluation of patients with low-tension glaucoma.

    abstract：:One hypothesized cause of low-tension glaucoma is chronic or intermittent ischemia of the optic nerve. Since the optic nerve and brain are both parts of the central nervous system and share a common blood supply, the authors wondered if patients with low-tension glaucoma might also have clinical or radiographic eviden...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Corbett JJ,Phelps CD,Eslinger P,Montague PR

    更新日期：1985-08-01 00:00:00

  • A model for the observer on the Farnsworth-Munsell 100-hue test.

    abstract：PURPOSE:To use a theoretical model of the observer on the Farnsworth-Munsell 100-hue test to estimate the magnitude of random variation in 100-Hue test error scores. METHODS:The model was based upon classical signal detection theory. Results from the model were obtained by computer simulation. RESULTS:There is a fair...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Craven BJ

    更新日期：1993-03-01 00:00:00

  • Changes in matrix metalloproteinase network in a spontaneous autoimmune uveitis model.

    abstract：PURPOSE:Autoimmune uveitis is a sight-threatening disease in which autoreactive T cells cross the blood-retinal barrier. Molecular mechanisms contributing to the loss of eye immune privilege in this autoimmune disease are not well understood. In this study, the authors investigated the changes in the matrix metalloprot...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-6475

    authors： Hofmaier F,Hauck SM,Amann B,Degroote RL,Deeg CA

    更新日期：2011-04-08 00:00:00

  • Effects of silicone hydrogel contact lens wear on ocular surface sensitivity to tactile, pneumatic mechanical, and chemical stimulation.

    abstract：PURPOSE:To determine the effects of silicone hydrogel lens wear and lens-solution interactions on ocular surface sensitivity. METHODS:Forty-eight adapted lens wearers completed the study, which comprised two phases. Phase 1 included habitual lens wear, no lens wear (7 ± 3 days), and balafilcon A lenses (PV; PureVision...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,随机对照试验

    doi：10.1167/iovs.09-4807

    authors： Situ P,Simpson TL,Jones LW,Fonn D

    更新日期：2010-12-01 00:00:00

  • Endogenic regulation of proliferation and zinc transporters by pigment epithelial cells nonvirally transfected with PEDF.

    abstract：PURPOSE:Genetic modification of cells before transplantation may allow the delivery of neuroprotective and other functional molecules to patients with neurodegenerative diseases. To avoid complications associated with virally transfected cells, we have explored the use of nonviral methods to insert genetic material int...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-6178

    authors： Johnen S,Kazanskaya O,Armogan N,Stickelmann C,Stöcker M,Walter P,Thumann G

    更新日期：2011-07-23 00:00:00

  • Voluntary Exercise Suppresses Choroidal Neovascularization in Mice.

    abstract：Purpose:To determine the effect of voluntary exercise on choroidal neovascularization (CNV) in mice. Methods:Age-matched wild-type C57BL/6J mice were housed in cages equipped with or without running wheels. After four weeks of voluntary running or sedentariness, mice were subjected to laser injury to induce CNV. After...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.61.5.52

    authors： Makin RD,Argyle D,Hirahara S,Nagasaka Y,Zhang M,Yan Z,Kerur N,Ambati J,Gelfand BD

    更新日期：2020-05-11 00:00:00

  • Differentiated Expression Patterns and Phagocytic Activities of Type 1 and 2 Microglia.

    abstract：PURPOSE:The purpose of this study was to elucidate the differentiated expression patterns and phagocytic activities of two subtypes of microglia. METHODS:A rat optic nerve crush model was used to identify the expression patterns of two subtypes of microglia. Primary microglia were isolated from rat mixed glial culture...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-18509

    authors： Haga A,Takahashi E,Inomata Y,Kawahara K,Tanihara H

    更新日期：2016-05-01 00:00:00

  • Regional microvascular anatomy of the rabbit ciliary body.

    abstract：:Vascular luminal castings of rabbit eyes were microdissected and studied with the scanning electron microscope to delineate the anatomy of the ciliary body microvasculature. We found regional microvascular differences corresponding to well known gross topographical and ultrastructural differences that may indicate reg...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Morrison JC,DeFrank MP,Van Buskirk EM

    更新日期：1987-08-01 00:00:00

  • Glaucoma Diagnostic Ability of the New Circumpapillary Retinal Nerve Fiber Layer Thickness Analysis Based on Bruch's Membrane Opening.

    abstract：PURPOSE:To compare the diagnostic ability of the new spectral-domain optical coherence tomography (SD-OCT) algorithm for measuring circumpapillary retinal nerve fiber layer (RNFL) thickness centered on Bruch's membrane opening (BMO), with the conventional circumpapillary RNFL thickness measurement centered on the optic...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-19578

    authors： Lee EJ,Lee KM,Kim H,Kim TW

    更新日期：2016-08-01 00:00:00

  • Effect of repeated anterior chamber perfusion on intraocular pressure and total outflow facility in the cynomolgus monkey.

    abstract：:Total outflow facility was determined by two-level constant pressure perfusion of the anterior chamber in surgically virgin, aniridic, and ciliary muscle disinserted cynomolgus monkey eyes 6 to 13 times at 1- to 2-month intervals over periods of 8 to 24 months. Facility decreased approximately 15 to 20% between consec...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Kaufman PL,Erickson KA,Bárány EH

    更新日期：1983-02-01 00:00:00

  • Subretinal neovascularization after naphthalene damage to the rabbit retina.

    abstract：PURPOSE:To examine the retinotoxic effect of naphthalene, a powerful oxidative agent and a well-known cataractogenic agent. METHODS:A 10% solution of naphthalene dissolved in paraffin oil was given every other day by gavage to 31 pigmented rabbits for 5 weeks, at a dose of 1 g/kg body weight. Four rabbits who received...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Orzalesi N,Migliavacca L,Miglior S

    更新日期：1994-02-01 00:00:00

  • Comparison of Visual Field Progression Rates Among the High Tension Glaucoma, Primary Angle Closure Glaucoma, and Normal Tension Glaucoma.

    abstract：Purpose:To compare the visual field (VF) progression among high tension glaucoma (HTG), primary angle closure glaucoma (PACG), and normal tension glaucoma (NTG) subjects in routine clinical care. Methods:All patients had ≥5 VF tests using HFA, 24-2, SITA-standard strategy. We compared the progression between glaucoma ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.18-25421

    authors： Ballae Ganeshrao S,Senthil S,Choudhari N,Sri Durgam S,Garudadri CS

    更新日期：2019-03-01 00:00:00

  • Increased plasma levels of substance P in vernal keratoconjunctivitis.

    abstract：PURPOSE:The increase of nerve growth factor (NGF) plasma levels in vernal keratoconjunctivitis (VKC) patients has been demonstrated previously. Results of numerous studies in vitro and in vivo have shown that NGF modulates the synthesis of substance P (SP), a neuropeptide involved in the pathogenesis of human allergic ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Lambiase A,Bonini S,Micera A,Tirassa P,Magrini L,Bonini S,Aloe L

    更新日期：1997-09-01 00:00:00

  • Enzymatic breakdown of type II collagen in the human vitreous.

    abstract：PURPOSE:To investigate whether enzymatic collagen breakdown is an active process in the human vitreous. METHODS:Human donor eyes were used for immunohistochemistry to detect the possible presence of the matrix metalloproteinase (MMP)-induced type II collagen breakdown product col2-3/4C-short in the vitreous. Western b...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.08-3125

    authors： van Deemter M,Pas HH,Kuijer R,van der Worp RJ,Hooymans JM,Los LI

    更新日期：2009-10-01 00:00:00

  • MRI of Retinal Free Radical Production With Laminar Resolution In Vivo.

    abstract：PURPOSE:Recent studies have suggested the hypothesis that quench-assisted 1/T1 magnetic resonance imaging (MRI) measures free radical production with laminar resolution in vivo without the need of a contrast agent. Here, we test this hypothesis further by examining the spatial and detection sensitivity of quench-assist...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-18972

    authors： Berkowitz BA,Lewin AS,Biswal MR,Bredell BX,Davis C,Roberts R

    更新日期：2016-02-01 00:00:00

  • Performance on the Farnsworth-Munsell 100-Hue Test Is Significantly Related to Nonverbal IQ.

    abstract：PURPOSE:The Farnsworth-Munsell 100-Hue test (FM100) is a standardized measure of chromatic discrimination, based on colored cap-sorting, which has been widely used in both adults and children. Its dependence on seriation ability raises questions as to its universal suitability and accuracy in assessing purely sensory d...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.14-16094

    authors： Cranwell MB,Pearce B,Loveridge C,Hurlbert AC

    更新日期：2015-05-01 00:00:00

  • Pharmacokinetics of pars plana intravitreal injections versus microcannula suprachoroidal injections of bevacizumab in a porcine model.

    abstract：PURPOSE:To compare the pharmacokinetics and tissue response between intravitreal and microcannulation injections into the suprachoroidal space using bevacizumab. METHODS:Sixty-two pigs were studied. Either a pars plana intravitreal bevacizumab or a viscoelastic-enhanced microcannula suprachoroidal injection was perfor...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-6291

    authors： Olsen TW,Feng X,Wabner K,Csaky K,Pambuccian S,Cameron JD

    更新日期：2011-07-01 00:00:00

  • Survival of donor-derived cells in human corneal transplants.

    abstract：PURPOSE:To determine the fate of donor epithelial, stromal, and endothelial cells after corneal transplantation in humans. METHODS:Fifty-two transplanted corneal buttons were explanted over a 2-year period from patients who required regrafting and had received corneas from donors of opposite sex. Fluorescence in situ ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.08-2923

    authors： Lagali N,Stenevi U,Claesson M,Fagerholm P,Hanson C,Weijdegård B,Swedish Society of Corneal Surgeons.

    更新日期：2009-06-01 00:00:00