Abstract：
:To assess the genetic diversity among four canola cultivars (namely, Serw-3, Serw-4, Misser L-16 and Semu 249), random amplified polymorphic DNA (RAPD), simple sequence repeat polymorphism (SSR) and amplified fragment length polymorphism (AFLP) analyses were performed. The data indicated that all of the three molecular markers gave different levels of polymorphism. A total of 118, 31 and 338 markers that show 61, 67.7 and 81 % polymorphism percentages were resulted from the RAPD, SSR and AFLP analyses, respectively. Based on the data obtained the three markers can be used to differentiate between the four canola cultivars. The genotype-specific markers were determined, 18 out of the 72 polymorphic RAPD markers generated were found to be genotype-specific (25 %). The highest number of RAPD specific markers was scored for Semu 249 (15 markers), while Serw-4 scored two markers. On the other hand, Serw-3 scored one marker. The cultivar Semu 249 scored the highest number of unique AFLP markers, giving 57 unique markers, followed by Misser L-16 which was characterized by 40 unique AFLP markers, then Serw-3 giving 31 unique markers. While Serw-4 was characterized by the lowest number producing 14 unique positive markers. The dendrogram built on the basis of combined data from RAPD, SSR and AFLP analysis represents the genetic distances among the four canola cultivars. Understanding the genetic variability among the current canola cultivars opens up a possibility for developing a molecular genetic map that will lead to the application of marker-assisted selection tools in genetic improvement of canola.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Moghaieb RE,Mohammed EH,Youssief SSdoi
10.1007/s13205-013-0165-xsubject
Has Abstractpub_date
2014-08-01 00:00:00pages
403-410issue
4eissn
2190-572Xissn
2190-5738pii
10.1007/s13205-013-0165-xjournal_volume
4pub_type
杂志文章相关文献
3 Biotech文献大全abstract：:An efficient in vitro regeneration system using epicotyl segments was developed and then used for optimizing genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis) variety using phosphinothricin (PPT) resistance as a selectable marker. The maximum regeneration efficiency was achieved after incub...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2097-6
更新日期：2020-03-01 00:00:00
abstract：:A soil bacterium, Bacillus subtilis, isolated from the rhizosphere of Chilli, showed high antagonistic activity against Colletotrichum gloeosporioides OGC1. A clear inhibition zone of 0.5-1 cm was observed in dual plate assay. Microscopic observations showed a clear hyphal lysis and degradation of fungal cell wall. In...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-013-0134-4
更新日期：2014-04-01 00:00:00
abstract：:In this study, the effect of pasteurization and use of starter cultures on physicochemical, microbiological and functional properties of a traditional Iranian semi-hard cheese (Lighvan cheese) was evaluated during stages of ripening (1, 60, 120 days). Profiles of polar metabolites were analyzed by gas-chromatography m...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-2075-z
更新日期：2020-03-01 00:00:00
abstract：:Cassava is an important food for millions of people around the world. However, cassava is deficient in protein, iron, zinc, pro-vitamin A and vitamin E. Cassava biofortified with pro-vitamin A can help reduce Vitamin A Deficiency among the undernourished communities that rely upon it for sustenance. BioCassava Plus pr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-014-0243-8
更新日期：2015-08-01 00:00:00
abstract：:Trehalose is a non-reducing disaccharide sugar that widely exists in a variety of organisms, such as bacteria and eukaryotes except the vertebrates. It plays an important role in a number of critical metabolic functions especially in response to stressful environmental conditions. However, the biosynthetic pathways of...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0983-3
更新日期：2017-10-01 00:00:00
abstract：:Human papilloma virus (HPV) is the primary etiological agent responsible for cervical cancer in women. Although in total 16 high-risk HPV strains have been identified so far. Currently available commercial vaccines are designed by targeting mainly HPV16 and HPV18 viral strains as these are the most common strains asso...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0352-z
更新日期：2016-06-01 00:00:00
abstract：:Gastric cancer is one of the most common cancers in modern societies. Previous studies have shown that the use of nanoparticle complexes is effective in the treatment of cancer. The aim of this study was to investigate the cytotoxicity and anticancer properties of cobalt oxide (Co3O4) nanoparticles (NPs) functionalize...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02230-4
更新日期：2020-05-01 00:00:00
abstract：:This study aimed to explore the expressions of signal transducer and activator of transcription 3 (STAT3) and a gene associated with retinoid-interferon induced mortality (Grim19) in epithelial ovarian cancer (EOC), and to determine their correlations with tumor progression and metastasis as well as the related mechan...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02237-x
更新日期：2020-06-01 00:00:00
abstract：:Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to achieve desirable level of resistance against target insects. In order to achieve desirable level of expression, rice chloroplast trans...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-015-0315-4
更新日期：2016-06-01 00:00:00
abstract：:Statistical experimental designs were used to optimize conditions for recombinant Burkholderia pyrrocinia feruloyl esterase (BpFae) production in bacteria under lactose induction. After optimization by single factor design, Plackett-Burman design, steepest ascent design and the response surface method, the optimal con...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02198-1
更新日期：2020-05-01 00:00:00
abstract：:Our earlier studies have indicated that GA3, being a growth hormone, increases internodal length, in turn increasing sink strength and improving sucrose accumulation in sugarcane. In this study, transcriptomic level analysis was carried out on internodal samples of a high sugar accumulating variety (CoLk 94184) of sug...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1908-0
更新日期：2019-10-01 00:00:00
abstract：:Several actinomycetes strains were isolated from different marine sponges collected from the Red Sea shore in Egypt. The efficiency of their crude extracts to inhibit histone deacetylase (HDAC) enzyme was investigated in the nuclear extract of Hela cell line. The crude extract corresponding to Streptomyces sp. SP9 iso...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1304-1
更新日期：2018-06-01 00:00:00
abstract：:Different agricultural residues were considered in this study for their ability to support cellulolytic enzyme production by Aspergillus niger. A total of eleven agricultural residues including finger millet hulls, sorghum hulls, soybean hulls, groundnut husk, banana peels, corn stalk, cassava peels, sugarcane bagasse...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-015-0294-5
更新日期：2015-12-01 00:00:00
abstract：:Chemically unique environment of endophytes makes them to have various adaptive mechanisms for survival. One of such mechanisms involves the production of pharmacologically significant plant-specific metabolites. In the present study, 26 endophytic fungi were isolated from stem of Bacopa monnieri (L.) Wettst. plants. ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0788-4
更新日期：2017-07-01 00:00:00
abstract：:Current study describes the green, environmental friendly, and cost-effectiveness technique for the preparation of MgO nanoparticles (NPs) via white button mushroom aqueous extract. The synthesized MgO NPs were characterized using equipments such as X-ray diffraction, dynamic light scattering (DLS), scanning electron ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0894-3
更新日期：2017-08-01 00:00:00
abstract：:In the present work, bioethanol was produced by sugar fermentation obtained from water hyacinth using a novelty hybrid method composed of steam explosion and enzymatic hydrolysis, using hydrolytic enzymes produced by solid-state fermentation and water hyacinth as substrate. The highest activity, 42 U for xylanase and ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02426-8
更新日期：2020-10-01 00:00:00
abstract：:2,4-dinitrotoluene (2,4-DNT) is a priority environmental xenobiotic pollutant which has toxic, mutagenic, and carcinogenic properties. Thus, its biodegradation by applying recent approaches such as taking advantage of plant-bacteria interactions is crucial. In this work, the genes from Burkholderia sp. R34, necessary ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-019-1933-z
更新日期：2019-11-01 00:00:00
abstract：:Viral diseases affecting sweetpotato are the most devastating and cause up to 98 % yield loss. In this paper, we report, meristem culture, graft transmission and virus indexing for management of viral pathogens in seven elite sweetpotato cultivars. Plantlets were developed in vitro from the apical meristematic dome wi...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-012-0080-6
更新日期：2013-04-01 00:00:00
abstract：:Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was select...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0764-z
更新日期：2017-06-01 00:00:00
abstract：:Bacillus velezensis LPL-K103, which shows strong antifungal function, was isolated from the surface of lemon in Beijing, China. The complete genome of B. velezensis LPL-K103 contains a circular chromosome of 3,933,292 bp (46.61% G+C content). According to genomic analysis, 4080 protein-coding genes, 113 RNAs (27 rRNAs...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-019-1995-y
更新日期：2020-01-01 00:00:00
abstract：:In fishes, trypsins are adapted to different environmental conditions, and the biochemical and kinetic properties of a broad variety of native isoforms have been studied. Proteolytic enzymes remain in high demand in the detergent, food, and feed industries; however, our analysis of the literature showed that, in the l...
journal_title：3 Biotech
pub_type： 杂志文章,评审
doi：10.1007/s13205-018-1208-0
更新日期：2018-04-01 00:00:00
abstract：:Within a decade, MERS-CoV emerged with nearly four times higher case fatality rate than an earlier outbreak of SARS-CoV and spread out in 27 countries in short span of time. As an emerging virus, combating it requires an in-depth understanding of its molecular machinery. Therefore, conformational characterization stud...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0744-3
更新日期：2017-06-01 00:00:00
abstract：:Several proteins and peptides of microbial origin are reported for their elicitor properties, which play a vital role in the development of local and systemic resistances in plants. In this study, the efficacy of total crude proteins (TCP) extracted from six different Trichoderma spp. (T. asperellum, T. harzianum, T. ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0816-4
更新日期：2017-07-01 00:00:00
abstract：:The current investigation is taken up with the aim of studying repeated batch and continuous degradation of Endosulfan, using Ca-alginate immobilized cells of Pseudomonas aeruginosa isolated from an agricultural soil. The work involves the study of genes and enzymes involved in the degradation of the pesticide and was...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-016-0438-2
更新日期：2016-12-01 00:00:00
abstract：:Parthenium hysterophorus is a noxious weed in America, Asia, Africa and Australia. This weed is considered to be a cause of allergic respiratory problems, contact dermatitis, mutagenicity in human and livestock. Crop production is drastically reduced owing to its allelopathy. Also aggressive dominance of this weed thr...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-011-0007-7
更新日期：2011-07-01 00:00:00
abstract：:The effect of water stress and herbicide treatment on the phenolic compound concentration and photosynthesis rate in transgenic Codonopsis lanceolata plants over-expressing the γ-tmt gene was investigated and compared to that in control non-transgenic C. lanceolata plants. The total phenolic compound content was inves...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-0795-5
更新日期：2017-07-01 00:00:00
abstract：:Isolation of high-quality RNA from weed plants such as Parthenium hysterophorus is a difficult task due to the hindrance caused by numerous secondary metabolites. Such metabolites not only affect the quality and yield of RNA, but also limit the quality of downstream applications. Therefore, the present study was under...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-017-1003-3
更新日期：2017-12-01 00:00:00
abstract：:In this work we evaluated methanolic extracts from different parts (leaves, seeds, fruit peel and pulp) of Chamaerops humilis L. for antioxidant activity and the ability to inhibit enzymes linked with neurodegenerative diseases: acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (TYR). The total ...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-018-1110-9
更新日期：2018-02-01 00:00:00
abstract：:Parasporal crystalline inclusion proteins of some Bacillus spp. are of paramount importance due to their insecticidal, nematocidal, and cancer cell killing capabilities. Here, we present a brief report of the complete genome sequence of Bacillus sp. BD59S, a bacterium that produced HeLa cell-killing parasporal crystal...
journal_title：3 Biotech
pub_type：
doi：10.1007/s13205-019-1849-7
更新日期：2019-09-01 00:00:00
abstract：:The electrochemical DNA biosensor has been developed for the detection of Listeria monocytogenes in raw milk samples. The electrochemical studies of the developed biosensor was recorded by cyclic voltammetry (CV) and electrochemical impedance (EI) using methylene blue (MB) and potassium ferricyanide K3Fe(CN)-6 as redo...
journal_title：3 Biotech
pub_type： 杂志文章
doi：10.1007/s13205-020-02315-0
更新日期：2020-07-01 00:00:00
