Abstract:
:Plodia interpunctella (Hübner) is an important stored grain insect pest worldwide, and the first lepidopteran with reported resistance to Bacillus thuringiensis (Bt) toxins. Since gut bacteria may affect Bt insecticidal activity, we evaluated whether P. interpunctella lacking gut bacteria had differences in immune responses and susceptibility to the Bt formulation, Bactospeine. In order to clear gut bacteria, third instar larvae were reared on artificial diet containing antibiotics, or were obtained from sterilized eggs and reared under sterile conditions, and larvae were fed diets with or without Bt. Mortality was significantly lower (p<0.05) in bacteria-free larvae treated with Bt, compared with Bt-treated larvae with unaffected gut bacteria. The number of hemocytes was lower in control and Bt-treated larvae, but was significantly higher (p<0.001) in larvae treated with antibiotics and Bt, and larvae from presterilized eggs and reared on sterile diet had the highest number of hemocytes. Phenoloxidase activity was significantly lower (p<0.05) in Bt-treated larvae from presterilized eggs reared on antibiotics for 24h or in larvae reared on antibiotic-treated diets prior to Bt introduction compared with those fed control diet. Hemolin gene expression was reduced in larvae fed Bt diets compared with control and was not detected in larvae treated with antibiotics. Larvae from sterilized eggs and fed sterile diet never reached the pupal stage. Therefore, the loss of gut bacteria in P. interpunctella larvae affected the host immune response and expression of the hemolin gene, and significantly reduced susceptibility to Bt.
journal_name
J Insect Physioljournal_title
Journal of insect physiologyauthors
Orozco-Flores AA,Valadez-Lira JA,Oppert B,Gomez-Flores R,Tamez-Guerra R,Rodríguez-Padilla C,Tamez-Guerra Pdoi
10.1016/j.jinsphys.2017.01.020subject
Has Abstractpub_date
2017-04-01 00:00:00pages
275-283eissn
0022-1910issn
1879-1611pii
S0022-1910(16)30308-0journal_volume
98pub_type
杂志文章abstract::Mating in moths is generally mediated by female-produced sex pheromones. Mating activity, female pheromone production/release and male pheromone responsiveness all show diurnal variations in many species. We found that the response of the male Egyptian cotton leafworm, Spodoptera littoralis, to sex pheromone gland ext...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2004.11.013
更新日期:2005-03-01 00:00:00
abstract::Larvae of Syrphus ribesii collected from overwintering sites in the U.K. are strongly freeze tolerant with 70% survival at -35 degrees C. The cold tolerance of laboratory reared insects increased with increasing periods of acclimation at 0 degrees C, with a concurrent rise in the supercooling point (SCP) from -6.8+/-0...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(97)00106-6
更新日期:1997-11-01 00:00:00
abstract::Classical studies have described in detail the complex and regionalized morphology of the Malpighian tubule (MT) in larval Lepidoptera. Recent studies revealed unusual aspects of ion transport in the Malpighian tubules of the larva of the cabbage looper, Trichoplusia ni. These included: cation reabsorption via seconda...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2018.06.005
更新日期:2018-01-01 00:00:00
abstract::It has been recently suggested that female mate choice, based on sexually selected ornaments, is an important component of social wasps' reproductive biology. The correlates of male ornaments that could be of a female's interest, however, remain to be investigated. Males of the Neotropical paper wasp Polistes simillim...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2018.06.002
更新日期:2018-01-01 00:00:00
abstract::We investigated if Brindley's and metasternal glands are involved in the sexual behavior of Triatoma infestans. In laboratory assays, we analyzed the effect of selective occlusion of Brindley's and metasternal glands of the female (separately and together) on the behavior of males. Control assays without occlusion of ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2007.03.014
更新日期:2007-07-01 00:00:00
abstract::First generation hybrid males from crosses between the Asian corn borer (ACB), Ostrinia furnacalis, and the "univoltine Z-strain" European corn borer (ECB), Ostrinia nubilalis, were examined with respect to behavioral and physiological responses to ACB and ECB pheromones. The hybrid males often flew to the pheromone o...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2009.09.005
更新日期:2010-01-01 00:00:00
abstract::We found that in the flesh fly Neobellieria bullata, vitellogenesis can be inhibited in a dose-dependent way by two injections of 60 microg MK-801/g body mass. In the desert locust Schistocerca gregaria, vitellogenesis can also be fully inhibited but only by repeated injections of 200-400 microg/g body mass. In this s...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2004.07.003
更新日期:2004-10-01 00:00:00
abstract::The quantity and composition of the six major mandibular gland components of young queenless workers of the Cape honeybee (Apis mellifera capensis) were determined. The total amount of the six components increased with age. The relative quantities in the mandibular gland secretion of queenless caged workers were found...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(00)00167-0
更新日期:2001-07-01 00:00:00
abstract::We studied behavioral responses of the cricket, Gryllus bimaculatus, to mechanical stimulation of the hindwing tip using three different kinds of stimuli: touching, bending and pinching. The most characteristic was a sequence of initial jump-like movements and subsequent running steps, that is referred to as escape be...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(00)00058-5
更新日期:2000-10-01 00:00:00
abstract::Egg-laying in Locusta migratoria involves the control of a variety of complex behavioural patterns including those that regulate digging of the oviposition hole and retention of eggs during digging. These two behavioural patterns are under the control of central pattern generators (CPGs). The digging and egg-retention...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2014.02.002
更新日期:2014-04-01 00:00:00
abstract::Haemocytes play crucial roles in immune responses and survival in insects. Specific cell markers have proven effective in clarifying the function and haematopoiesis of haemocytes. The silkworm Bombyx mori is a good model for studying insect haemocytes; however, little is known about haemocyte-specific markers or their...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2014.10.007
更新日期:2014-12-01 00:00:00
abstract::Juvenile hormone (JH) regulates diverse physiological processes in insects during entire developmental stages. Especially, the identification of Methoprene-tolerant (Met), a JH nuclear receptor, allows us to better understand molecular actions of JH to control gene expressions related with metamorphosis. However, seve...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2014.02.008
更新日期:2014-04-01 00:00:00
abstract::Juvenile hormones (JH) I, II, and III were monitored in hemolymph of virgin and mated females of various ages in Heliothis virescens. JH I was the predominant homologue followed by JH II, but JH II was present at a higher level in young virgin females. JH III was detectable only at a low level. In virgin females, hemo...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(97)00117-0
更新日期:1998-11-01 00:00:00
abstract::The oviposition of female locusts is a complex behaviour that includes a dramatic extension of the abdomen. The role of internal pressure during oviposition was investigated by monitoring the intra-tracheal pressure and the activity of selected longitudinal muscles, while movements of the abdomen were visualised with ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(99)00103-1
更新日期:2000-01-01 00:00:00
abstract::The juvenile hormone (JH) analog methoprene reduces the amount of time it takes laboratory-reared Anastrepha suspensa (Caribbean fruit fly) males to reach sexual maturity by almost half. Here, we examined if methoprene exerted a similar effect on four other tropical Anastrepha species (Anastrepha ludens, Anastrepha ob...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2008.11.013
更新日期:2009-03-01 00:00:00
abstract::Body size and shape affect thermoregulatory properties of organisms, and in turn are believed to have shaped macroevolutionary patterns of morphological diversity across many taxa. However, it is less clear whether thermoregulation plays a role in shaping intraspecific morphological diversity such as sexual dimorphism...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2007.12.007
更新日期:2008-03-01 00:00:00
abstract::Antisera against the pigment-dispersing factor (PDF) and corazonin (Crz) reacted with distinct sets of neurons in the cephalic ganglia of termites. The locations of immunoreactive cells were similar but their numbers differed among the eight species examined: PDF-ir occurred in 0-6 cells in each optic lobe and 1-2 pai...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2008.11.009
更新日期:2009-05-01 00:00:00
abstract::Prey nutrient quality determines predator performance. Polyphagous predators can address nutritional challenges by targeting prey with specific nutrient composition, but prey-specialised predators (e.g., ant-eaters), must obtain all nutrients from limited array of prey. Analysis of published data on prey specificity o...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2014.01.008
更新日期:2014-03-01 00:00:00
abstract::Force feeding of RH-5992 (Tebufenozide), a non-steroidal ecdysone agonist to newly moulted sixth instar larvae of the spruce budworm, Choristoneura fumiferana, (Lepidoptera: Tortricidae) initiates a precocious, incomplete moult. Within 6h post treatment (pt) the larva stops feeding and remains quiescent. Around 12hpt,...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(96)00062-5
更新日期:1997-02-19 00:00:00
abstract::Diapause is an alternate developmental pathway that is regulated by the neuroendocrine system in insects. To date, much of the information that has been published regarding the possible molecular events associated with diapause have been at the level of transcription. However, since transcription and translation are n...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2011.03.022
更新日期:2011-05-01 00:00:00
abstract::After the emergence of the Ceratitis capitata imago, the pale and folded wings are expanded and sclerotized to acquire the definitive form and to stabilize the cuticle. The wings of this fly show a specific pattern of brownish and black spots. Black spots are pigmented by melanin, whereas there was scarce information ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2018.04.010
更新日期:2018-01-01 00:00:00
abstract::The effect of plant nitrogen (N) status on the content and distribution of free amino acids in the bodies and honeydew of silverleaf whiteflies Bemisia tabaci (Gennadius) Biotype B (= B. argentifolii Bellows and Perring) was determined. Whiteflies fed for 4 days on cotton leaves that received high or low N fertility. ...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/s0022-1910(01)00140-8
更新日期:2002-01-01 00:00:00
abstract::Social environments can have a major impact on ageing profiles in many animals. However, such patterns in variation in ageing and their underlying mechanisms are not well understood, particularly because both social contact and isolation can be stressful. Here, we use Drosophila melanogaster fruitflies to examine sex-...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2017.08.008
更新日期:2017-10-01 00:00:00
abstract::This study gives the first electrophysiological evidence of hygroreceptors in carabids. Extracellular recordings from the antennal dome-shaped sensilla of the carabid beetle Pterostichus oblolongopunctatus (Coleoptera, Carabidae) clearly show the presence of moist and dry neuron antagonistically responding to humidity...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2010.06.017
更新日期:2010-11-01 00:00:00
abstract::Larvae of endoparasitoids undergo extensive morphological changes and often have special features to allow their development inside the host. We present the first detailed study on the development of the anal vesicle and the gut. The analyses reveal that the anal vesicle is first seen on the dorsal side of the abdomen...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2005.11.006
更新日期:2006-03-01 00:00:00
abstract::To understand the inhibitory effects of high temperature on insect growth at the cellular level, we investigated the influence of high temperature on the proliferation and division of larval hemocytes in the silkworm, Bombyx mori. Although the total number of hemocytes in the larval body increased enormously over time...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2007.11.004
更新日期:2008-02-01 00:00:00
abstract::A mechanistic understanding of the butterfly wing color-pattern determination can be facilitated by experimental pattern changes. Here I review physiologically induced color-pattern changes in nymphalid butterflies and their mechanistic and evolutionary implications. A type of color-pattern change can be elicited by e...
journal_title:Journal of insect physiology
pub_type: 杂志文章,评审
doi:10.1016/j.jinsphys.2008.05.006
更新日期:2008-07-01 00:00:00
abstract::The linden bug Pyrrhocoris apterus exhibits a robust diapause response to photoperiod. Photoperiod strongly affected basal levels of circadian gene transcripts in the gut, via the neuroendocrine system. Cryptochrome 2 (cry2) mRNA level was much higher in diapause promoting short days (SD) than in reproduction promotin...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2013.06.004
更新日期:2013-09-01 00:00:00
abstract::Female mosquitoes require blood meals to complete oogenesis, or egg development. Current methods of maintaining laboratory colonies of mosquitoes generally rely on the use of whole blood to feed females. Blood feeding protocols require special handling techniques, impart numerous potential health hazards, involve sign...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2014.02.012
更新日期:2014-05-01 00:00:00
abstract::In the honeybee Apis mellifera, a sting pheromone produced by sting glands plays an important role in coordinating defensive behavior. This pheromone is a blend constituted by several components. Little is known about the neural substrates underlying sting pheromone processing in the bee brain. Here, we investigated t...
journal_title:Journal of insect physiology
pub_type: 杂志文章
doi:10.1016/j.jinsphys.2008.03.004
更新日期:2008-05-01 00:00:00