Abstract:
:Cholesterol precursors and cholesterol levels are reduced in brain regions of Huntington's disease (HD) mice. Here we quantified the rate of in vivo de novo cholesterol biosynthesis in the HD brain. Samples from different brain regions and blood of the heterozygous knock-in mouse model carrying 175 CAG repeats (Q175) at different phenotypic stages were processed independently by two research units to quantify cholesterol synthesis rate by 2H2O labeling and measure the concentrations of lathosterol, cholesterol and its brain-specific cholesterol catabolite 24-hydroxy-cholesterol (24OHC) by isotope dilution mass spectrometry. The daily synthesis rate of cholesterol and the corresponding concentration of lathosterol were significantly reduced in the striatum of heterozygous Q175 mice early in the disease course. We also report that the decrease in lathosterol was inversely correlated with CAG-size at symptomatic stage, as observed in striatal samples from an allelic series of HD mice. There was also a significant correlation between the fractional synthesis rates of total cholesterol and 24OHC in brain of wild-type (WT) and Q175 mice, supporting the evidence that plasma 24OHC may reflect cholesterol synthesis in the adult brain. This comprehensive analysis demonstrates consistent cholesterol biosynthesis defects in HD mouse models and suggests that plasma 24OHC may serve as a biomarker of brain cholesterol metabolism.
journal_name
Neurobiol Disjournal_title
Neurobiology of diseaseauthors
Shankaran M,Di Paolo E,Leoni V,Caccia C,Ferrari Bardile C,Mohammed H,Di Donato S,Kwak S,Marchionini D,Turner S,Cattaneo E,Valenza Mdoi
10.1016/j.nbd.2016.11.013subject
Has Abstractpub_date
2017-02-01 00:00:00pages
66-76eissn
0969-9961issn
1095-953Xpii
S0969-9961(16)30281-9journal_volume
98pub_type
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