Individual cells in the raphe nuclei of the medulla oblongata in rat that contain immunoreactivities for both serotonin and enkephalin project to the spinal cord.

Abstract:

:The ventral medulla oblongata of rats was analyzed with a double-labelling immunofluorescence technique using guinea pig antibodies directed against serotonin (5-HT) and rabbit antisera directed against enkephalin (ENK). Numerous cells in the region of nucleus raphe obscurus, nucleus raphe pallidus and nucleus raphe magnus showed immunostaining for either 5-HT or ENK. A substantial number of cells showed positive immunostaining for both 5-HT and ENK. 5-HT/ENK double-labelled cells were most frequently encountered in an area that extended from the rostral aspect of the inferior olivary nucleus to the pontomedullary border. This region corresponds anatomically to nucleus raphe magnus/nucleus paragigantocellularis. In addition, a number of the 5-HT/ENK-containing cells were retrogradely labelled with Fluoro-Gold dye that had been injected into the thoracic spinal cord several days prior to perfusion. Schematic drawings showing the anatomical distribution of 5-HT/ENK colocalization are provided.

journal_name

Exp Brain Res

authors

Millhorn DE,Hökfelt T,Verhofstad AA,Terenius L

doi

10.1007/BF00249904

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

536-42

issue

3

eissn

0014-4819

issn

1432-1106

journal_volume

75

pub_type

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