Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO4.

Abstract:

:Knowledge of the relative contributions of different water sources to intracellular fluids and body water is important for many fields of study, ranging from animal physiology to paleoclimate. The intracellular fluid environment of cells is challenging to study due to the difficulties of accessing and sampling the contents of intact cells. Previous studies of multicelled organisms, mostly mammals, have estimated body water composition-including metabolic water produced as a byproduct of metabolism-based on indirect measurements of fluids averaged over the whole organism (e.g., blood) combined with modeling calculations. In microbial cells and aquatic organisms, metabolic water is not generally considered to be a significant component of intracellular water, due to the assumed unimpeded diffusion of water across cell membranes. Here we show that the (18)O/(16)O ratio of PO4 in intracellular biomolecules (e.g., DNA) directly reflects the O isotopic composition of intracellular water and thus may serve as a probe allowing direct sampling of the intracellular environment. We present two independent lines of evidence showing a significant contribution of metabolic water to the intracellular water of three environmentally diverse strains of bacteria. Our results indicate that ∼30-40% of O in PO4 comprising DNA/biomass in early stationary phase cells is derived from metabolic water, which bolsters previous results and also further suggests a constant metabolic water value for cells grown under similar conditions. These results suggest that previous studies assuming identical isotopic compositions for intracellular/extracellular water may need to be reconsidered.

authors

Li H,Yu C,Wang F,Chang SJ,Yao J,Blake RE

doi

10.1073/pnas.1521038113

subject

Has Abstract

pub_date

2016-05-24 00:00:00

pages

5862-7

issue

21

eissn

0027-8424

issn

1091-6490

pii

1521038113

journal_volume

113

pub_type

杂志文章
  • Switching substrate preference of thermophilic xylose isomerase from D-xylose to D-glucose by redesigning the substrate binding pocket.

    abstract::The substrate specificity of thermophilic xylose isomerase from Clostridium thermosulfurogenes was examined by using predictions from the known crystal structure of the Arthrobacter enzyme and site-directed mutagenesis of the thermophile xylA gene. The orientation of glucose as a substrate in the active site of the th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.9.4015

    authors: Meng M,Lee C,Bagdasarian M,Zeikus JG

    更新日期:1991-05-01 00:00:00

  • Proteomic analysis of skeletal organic matrix from the stony coral Stylophora pistillata.

    abstract::It has long been recognized that a suite of proteins exists in coral skeletons that is critical for the oriented precipitation of calcium carbonate crystals, yet these proteins remain poorly characterized. Using liquid chromatography-tandem mass spectrometry analysis of proteins extracted from the cell-free skeleton o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1301419110

    authors: Drake JL,Mass T,Haramaty L,Zelzion E,Bhattacharya D,Falkowski PG

    更新日期:2013-03-05 00:00:00

  • Sterically stabilized liposomes: improvements in pharmacokinetics and antitumor therapeutic efficacy.

    abstract::The results obtained in this study establish that liposome formulations incorporating a synthetic polyethylene glycol-derivatized phospholipid have a pronounced effect on liposome tissue distribution and can produce a large increase in the pharmacological efficacy of encapsulated antitumor drugs. This effect is substa...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.24.11460

    authors: Papahadjopoulos D,Allen TM,Gabizon A,Mayhew E,Matthay K,Huang SK,Lee KD,Woodle MC,Lasic DD,Redemann C

    更新日期:1991-12-15 00:00:00

  • Role of gender, season, and familiarity in discrimination of conspecific song by zebra finches (Taeniopygia guttata).

    abstract::Operant conditioning techniques were used to assess how gender and song familiarity affect song discrimination in adult zebra finches (Taeniopygia guttata). Twenty-five males and females, divided into five cohorts, were trained to discriminate between conspecific songs at different times of year. Males discriminating ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.4.1368

    authors: Cynx J,Nottebohm F

    更新日期:1992-02-15 00:00:00

  • Kinetoplast DNA minicircles: regions of extensive sequence divergence.

    abstract::Previous work has shown that the kinetoplast minicircle DNA of Leishmania species exhibits species-specific sequence divergence and this observation has led to the development of a DNA probe-based diagnostic test for leishmaniasis. In the work reported here, we demonstrate that the minicircle is composed of three type...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.2.565

    authors: Rogers WO,Wirth DF

    更新日期:1987-01-01 00:00:00

  • Mutations that increase the activity of a transcriptional activator in yeast and mammalian cells.

    abstract::Activating region I of GAL4 protein is a stretch of amino acids, positioned adjacent to the DNA-binding region, that activates transcription in yeast and, as we show here, in mammalian cells. Here we describe mutations located throughout a 65-amino acid region that increase the activation function of region I. Most of...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.6.2127

    authors: Gill G,Sadowski I,Ptashne M

    更新日期:1990-03-01 00:00:00

  • Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells.

    abstract::The activation of cyclin-dependent kinases (cdks) has been implicated in apoptosis induced by various stimuli. We find that the Fas-induced activation of cdc2 and cdk2 in Jurkat cells is not dependent on protein synthesis, which is shut down very early during apoptosis before caspase-3 activation. Instead, activation ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.12.6785

    authors: Zhou BB,Li H,Yuan J,Kirschner MW

    更新日期:1998-06-09 00:00:00

  • Functional traits predict relationship between plant abundance dynamic and long-term climate warming.

    abstract::Predicting climate change impact on ecosystem structure and services is one of the most important challenges in ecology. Until now, plant species response to climate change has been described at the level of fixed plant functional types, an approach limited by its inflexibility as there is much interspecific functiona...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1310700110

    authors: Soudzilovskaia NA,Elumeeva TG,Onipchenko VG,Shidakov II,Salpagarova FS,Khubiev AB,Tekeev DK,Cornelissen JH

    更新日期:2013-11-05 00:00:00

  • Archaeological and chemical evidence for early salt production in China.

    abstract::Salt production and trade is thought to be critical to the development of all states and emergent empires. Until now, however, scientific evidence of early salt production has rarely been presented, and no studies of early Chinese salt production have provided unequivocal proof. Here, we report x-ray fluorescence, x-r...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0502985102

    authors: Flad R,Zhu J,Wang C,Chen P,von Falkenhausen L,Sun Z,Li S

    更新日期:2005-08-30 00:00:00

  • Inhibition of the Mg(II).ATP-dependent phosphoprotein phosphatase by the regulatory subunit of cAMP-dependent protein kinase.

    abstract::We report potent inhibition of the Mg(II).ATP-dependent protein phosphatase, Fc.M, by the regulatory subunit dimer of type II cAMP-dependent protein kinase, RII2. The protein kinase catalytic subunit has no effect on phosphatase activity and is unable to substitute for kinase FA in the kinase FA- and Mg(II).ATP-mediat...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.22.7565

    authors: Jurgensen SR,Chock PB,Taylor S,Vandenheede JR,Merlevede W

    更新日期:1985-11-01 00:00:00

  • Existence of nonarithmetic monodromy groups.

    abstract::In an 1885 paper, E. Picard defined a subgroup Tau(Lambda) of PU(2,1) generated by monodromies and depending on parameters Lambda = (lambda(1),lambda(2),lambda(3),lambda(4)), 0 < lambda(i) < 1, < lambda(i) < 3, lambda(i) + lambda(j) >/= 1, 1

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.10.5948

    authors: Mostow GD

    更新日期:1981-10-01 00:00:00

  • Noninvasive detection of cancer-associated genome-wide hypomethylation and copy number aberrations by plasma DNA bisulfite sequencing.

    abstract::We explored the detection of genome-wide hypomethylation in plasma using shotgun massively parallel bisulfite sequencing as a marker for cancer. Tumor-associated copy number aberrations (CNAs) could also be observed from the bisulfite DNA sequencing data. Hypomethylation and CNAs were detected in the plasma DNA of pat...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1313995110

    authors: Chan KC,Jiang P,Chan CW,Sun K,Wong J,Hui EP,Chan SL,Chan WC,Hui DS,Ng SS,Chan HL,Wong CS,Ma BB,Chan AT,Lai PB,Sun H,Chiu RW,Lo YM

    更新日期:2013-11-19 00:00:00

  • The FKBP-associated protein FAP48 is an antiproliferative molecule and a player in T cell activation that increases IL2 synthesis.

    abstract::FAP48 was identified and cloned thanks to its interaction with FK506-binding proteins (FKBPs) such as FKBP52 and FKBP12, which belong to the large family of immunophilins that bind the macrolide immunosuppressant drugs FK506 and rapamycin. We have previously shown that FAP48-FKBP complexes are dissociated by FK506 and...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0438007100

    authors: Krummrei U,Baulieu EE,Chambraud B

    更新日期:2003-03-04 00:00:00

  • MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer.

    abstract::Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate gr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1203339109

    authors: Chaika NV,Gebregiworgis T,Lewallen ME,Purohit V,Radhakrishnan P,Liu X,Zhang B,Mehla K,Brown RB,Caffrey T,Yu F,Johnson KR,Powers R,Hollingsworth MA,Singh PK

    更新日期:2012-08-21 00:00:00

  • Beta 2-microglobulin gene mutations: a study of established colorectal cell lines and fresh tumors.

    abstract::The technique of single-strand conformation polymorphism (SSCP) was used to screen a series of 37 established colorectal cell lines, 22 fresh tumor samples, and 22 normal DNA samples for mutations in the beta 2-microglobulin gene. Exon 1 (including the leader peptide sequence) and exon 2 were screened separately. Six ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.11.4751

    authors: Bicknell DC,Rowan A,Bodmer WF

    更新日期:1994-05-24 00:00:00

  • Molecular and structural architecture of polyQ aggregates in yeast.

    abstract::Huntington's disease is caused by the expansion of a polyglutamine (polyQ) tract in the N-terminal exon of huntingtin (HttEx1), but the cellular mechanisms leading to neurodegeneration remain poorly understood. Here we present in situ structural studies by cryo-electron tomography of an established yeast model system ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1717978115

    authors: Gruber A,Hornburg D,Antonin M,Krahmer N,Collado J,Schaffer M,Zubaite G,Lüchtenborg C,Sachsenheimer T,Brügger B,Mann M,Baumeister W,Hartl FU,Hipp MS,Fernández-Busnadiego R

    更新日期:2018-04-10 00:00:00

  • BCL2-mediated tumorigenicity of a human T-lymphoid cell line: synergy with MYC and inhibition by BCL2 antisense.

    abstract::A gene-transfer approach was used to explore the function of the BCL2 (B-cell lymphoma/leukemia 2) gene in a human T-cell line, Jurkat. Though stable introduction of a BCL2 expression plasmid into Jurkat T cells was by itself insufficient, the combined transfer of BCL2 and MYC genes markedly enhanced the tumorigenicit...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.10.3660

    authors: Reed JC,Cuddy M,Haldar S,Croce C,Nowell P,Makover D,Bradley K

    更新日期:1990-05-01 00:00:00

  • MLL repression domain interacts with histone deacetylases, the polycomb group proteins HPC2 and BMI-1, and the corepressor C-terminal-binding protein.

    abstract::The MLL (mixed-lineage leukemia) gene is involved in many chromosomal translocations associated with acute myeloid and lymphoid leukemia. We previously identified a transcriptional repression domain in MLL, which contains a region with homology to DNA methyltransferase. In chromosomal translocations, the MLL repressio...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1436338100

    authors: Xia ZB,Anderson M,Diaz MO,Zeleznik-Le NJ

    更新日期:2003-07-08 00:00:00

  • Anterior hypothalamic vasopressin regulates pair-bonding and drug-induced aggression in a monogamous rodent.

    abstract::After pair-bonding, male prairie voles (Microtus ochrogaster) display aggression toward novel females but not toward their female partner. Here we show that this selective aggression in pair-bonded male prairie voles is associated with increased release of vasopressin (AVP) in the anterior hypothalamus (AH). Pharmacol...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0908620106

    authors: Gobrogge KL,Liu Y,Young LJ,Wang Z

    更新日期:2009-11-10 00:00:00

  • Chlorophyll and carotenoid binding in a simple red algal light-harvesting complex crosses phylogenetic lines.

    abstract::The membrane proteins of peripheral light-harvesting complexes (LHCs) bind chlorophylls and carotenoids and transfer energy to the reaction centers for photosynthesis. LHCs of chlorophytes, chromophytes, dinophytes, and rhodophytes are similar in that they have three transmembrane regions and several highly conserved ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.031587198

    authors: Grabowski B,Cunningham FX Jr,Gantt E

    更新日期:2001-02-27 00:00:00

  • Gene expression patterns define novel roles for E47 in cell cycle progression, cytokine-mediated signaling, and T lineage development.

    abstract::In maturing T lineage cells, the helix-loop-helix protein E47 has been shown to enforce a critical proliferation and developmental checkpoint commonly referred to as beta selection. To examine how E47 regulates cellular expansion and developmental progression, we have used an E2A-deficient lymphoma cell line and DNA m...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0603728103

    authors: Schwartz R,Engel I,Fallahi-Sichani M,Petrie HT,Murre C

    更新日期:2006-06-27 00:00:00

  • Genome sequence of Halobacterium species NRC-1.

    abstract::We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes. The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.190337797

    authors: Ng WV,Kennedy SP,Mahairas GG,Berquist B,Pan M,Shukla HD,Lasky SR,Baliga NS,Thorsson V,Sbrogna J,Swartzell S,Weir D,Hall J,Dahl TA,Welti R,Goo YA,Leithauser B,Keller K,Cruz R,Danson MJ,Hough DW,Maddocks DG,Jabl

    更新日期:2000-10-24 00:00:00

  • Partial amino acid sequence of porcine 1,25-dihydroxyvitamin D3 receptor isolated by immunoaffinity chromatography.

    abstract::Monoclonal antibodies against the porcine 1,25-dihydroxyvitamin D3 receptor were immobilized on Sepharose CL-4B and used to obtain a highly purified 1,25-dihydroxyvitamin D3 receptor fraction with a 45% recovery of the 1,25-dihydroxyvitamin D3 binding capacity. The porcine receptor was purified to homogeneity by prepa...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.8.2454

    authors: Brown TA,Prahl JM,DeLuca HF

    更新日期:1988-04-01 00:00:00

  • Quantitative imaging of free and total intracellular calcium in cultured cells.

    abstract::Techniques of fluorescence and ion microscopies were combined to study the free [Ca2+] and total Ca in NIH 3T3 fibroblast and L6 rat myoblast cells. Free Ca2+ measurements with the Ca2+ indicator fura-2 and digital imaging reveal an inhomogeneous distribution of free cytoplasmic Ca2+ in both cell lines. Fura-2 also re...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.6.1870

    authors: Chandra S,Gross D,Ling YC,Morrison GH

    更新日期:1989-03-01 00:00:00

  • Flat reversion by okadaic acid of raf and ret-II transformants.

    abstract::Okadaic acid is a non-phorbol 12-myristate 13-acetate (PMA)-type tumor promoter on mouse skin and known to be a potent inhibitor of serine/threonine protein phosphatases. Contrary to expectation from its tumor-promoting activity, okadaic acid was shown to have a potential to revert the phenotypes of cells transformed ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.24.9946

    authors: Sakai R,Ikeda I,Kitani H,Fujiki H,Takaku F,Rapp U,Sugimura T,Nagao M

    更新日期:1989-12-01 00:00:00

  • The heteromeric Nanoarchaeum equitans splicing endonuclease cleaves noncanonical bulge-helix-bulge motifs of joined tRNA halves.

    abstract::Among the tRNA population of the archaeal parasite Nanoarchaeum equitans are five species assembled from separate 5' and 3' tRNA halves and four species derived from tRNA precursors containing introns. In both groups an intervening sequence element must be removed during tRNA maturation. A bulge-helix-bulge (BHB) moti...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0509197102

    authors: Randau L,Calvin K,Hall M,Yuan J,Podar M,Li H,Söll D

    更新日期:2005-12-13 00:00:00

  • A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.

    abstract::A protein-free nucleic acid preparation method for electron microscopy is described. The basic procedure is very similar to the classical protein monolayer spreading techniques. The carrier protein (usually cytochrome c) is replaced by benzyldimethylalkylammonium chloride. Both the hypophase method and the microdiffus...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.1.83

    authors: Vollenweider HJ,Sogo JM,Koller T

    更新日期:1975-01-01 00:00:00

  • Evidence for neural rhythms embedded within binocular rivalry.

    abstract::Evidence for perceptual periodicity emerges from studies showing periodic fluctuations in visual perception and decision making that are accompanied by neural oscillations in brain activity. We have uncovered signs of periodicity in the time course of binocular rivalry, a widely studied form of multistable perception....

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1905174116

    authors: Cha O,Blake R

    更新日期:2019-07-23 00:00:00

  • Inhibition of proteolysis protects hippocampal neurons from ischemia.

    abstract::Intense proteolysis of cytoskeletal proteins occurs in brain within minutes of transient ischemia, possibly because of the activation of calcium-sensitive proteases (calpains). This proteolytic event precedes overt signs of neuronal degeneration, is most pronounced in regions of selective neuronal vulnerability, and c...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.16.7233

    authors: Lee KS,Frank S,Vanderklish P,Arai A,Lynch G

    更新日期:1991-08-15 00:00:00

  • Regulation of outer kinetochore Ndc80 complex-based microtubule attachments by the central kinetochore Mis12/MIND complex.

    abstract::Multiple protein subcomplexes of the kinetochore cooperate as a cohesive molecular unit that forms load-bearing microtubule attachments that drive mitotic chromosome movements. There is intriguing evidence suggesting that central kinetochore components influence kinetochore-microtubule attachment, but the mechanism re...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1513882112

    authors: Kudalkar EM,Scarborough EA,Umbreit NT,Zelter A,Gestaut DR,Riffle M,Johnson RS,MacCoss MJ,Asbury CL,Davis TN

    更新日期:2015-10-13 00:00:00